Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,488 G→A 6.0% E124K (GAA→ACA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,4880G→A 6.0% 22.4 ‑0.6 ‑0.0199E124K (GAA→AAA) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/5):  ref base (89/98):  total (96/103)
Fisher's exact test strand distribution p-value = 5.58e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.45e-01

Only 100 of 216 total aligned reads displayed.

GCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGG  >  NC_014034/1396388‑1396588
                                                                                                    |                                                                                                      
gCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCggg                                                                                                        <  1:3724520/101‑1 (MQ=255)
gCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCggg                                                                                                        >  1:1381022/1‑101 (MQ=255)
 cGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGa                                                                                                       <  1:2428197/101‑1 (MQ=255)
 cGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGa                                                                                                       <  2:430554/101‑1 (MQ=255)
  gTGGATCGAACCCGCGCCCTGCGGGTGATGGCGGCGGGCACCCCGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGaa                                                                                                      <  2:2552390/101‑1 (MQ=255)
  gTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGaa                                                                                                      >  1:3788082/1‑101 (MQ=255)
  gTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGaa                                                                                                      <  2:2820795/101‑1 (MQ=255)
   tcgATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGaaa                                                                                                     <  2:3355306/99‑1 (MQ=255)
   tGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGaaa                                                                                                     >  2:1954707/1‑101 (MQ=255)
    ggATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAAc                                                                                                    >  1:2924278/1‑101 (MQ=255)
        cGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACgcgg                                                                                                >  1:2652948/1‑101 (MQ=255)
         gAAACCGCGCCCTGCGGGGGATGGCGACGGGCCCCACGGCGGACGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTGCGCGGGAAACgcggc                                                                                               >  2:1887577/1‑101 (MQ=38)
              cgcgCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTTCGCGGGAAACGCGGCGGCGa                                                                                          <  2:287159/101‑1 (MQ=255)
              cgcgCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGa                                                                                          <  1:1825534/101‑1 (MQ=255)
              cgcgCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGa                                                                                          >  2:2764052/1‑101 (MQ=255)
                cgcCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGc                                                                                        <  1:1969301/101‑1 (MQ=255)
                cgcCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGc                                                                                        <  1:3449588/101‑1 (MQ=255)
                  cccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAc                                                                                      >  1:1745962/1‑101 (MQ=255)
                     tGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTc                                                                                  >  2:2573938/1‑101 (MQ=255)
                     tGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTc                                                                                  <  2:1402369/101‑1 (MQ=255)
                     tGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTc                                                                                  <  1:4182671/101‑1 (MQ=255)
                          gTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcc                                                                             <  1:3594334/101‑1 (MQ=255)
                            gATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccc                                                                           >  2:961341/1‑101 (MQ=255)
                             aTGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTccccc                                                                          >  1:2523448/1‑101 (MQ=255)
                             aTGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTccccc                                                                          <  2:2461445/101‑1 (MQ=255)
                              tGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccccc                                                                         <  1:1687492/101‑1 (MQ=255)
                              tGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccccc                                                                         <  1:1901757/101‑1 (MQ=255)
                                   aCGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCggg                                                                    <  1:4089090/101‑1 (MQ=255)
                                    cGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCgggg                                                                   >  1:2450056/1‑101 (MQ=255)
                                     gggCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCggggg                                                                  >  1:2016992/1‑101 (MQ=255)
                                     gggCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCggggg                                                                  >  1:2253151/1‑101 (MQ=255)
                                      ggCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGc                                                                 <  1:1633219/101‑1 (MQ=255)
                                      ggCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGc                                                                 <  1:698935/101‑1 (MQ=255)
                                       gCACCCCGGCGGTGGCTCCCCCCGCGGGGCCGGGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCg                                                                <  1:1503990/101‑1 (MQ=39)
                                       gCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCg                                                                >  1:1546318/1‑101 (MQ=255)
                                          ccacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTgg                                                             <  2:595506/101‑1 (MQ=255)
                                          ccacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTgg                                                             <  1:1218357/101‑1 (MQ=255)
                                           cacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                            >  2:3910711/1‑101 (MQ=255)
                                           cacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                            <  1:1188009/101‑1 (MQ=255)
                                            acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                           >  1:1351946/1‑101 (MQ=255)
                                             cggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                          >  2:871641/1‑101 (MQ=255)
                                             cggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  1:2094358/101‑1 (MQ=255)
                                              gtcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         <  1:987948/99‑1 (MQ=255)
                                              gggggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         <  1:100145/98‑1 (MQ=255)
                                              ggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         <  1:3685637/101‑1 (MQ=255)
                                              ggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         >  1:3150008/1‑101 (MQ=255)
                                              ggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAGCg                                                         >  2:3107921/1‑101 (MQ=255)
                                                cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       >  2:2250159/1‑101 (MQ=255)
                                                 cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                      <  1:1405040/100‑1 (MQ=255)
                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                     >  1:3991735/1‑101 (MQ=255)
                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                     >  1:2386422/1‑101 (MQ=255)
                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                    <  1:961432/101‑1 (MQ=255)
                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                                   >  2:3372833/1‑101 (MQ=255)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                                  <  2:1546214/101‑1 (MQ=255)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctg                                                 <  2:777018/101‑5 (MQ=0)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctg                                                 <  1:2315750/101‑5 (MQ=0)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctggc                                               >  1:2155958/1‑95 (MQ=0)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctggc                                               <  2:161006/101‑7 (MQ=0)
                                                        ccccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCttt                                               <  1:109823/101‑1 (MQ=255)
                                                        ccccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCttt                                               >  1:4020715/1‑101 (MQ=255)
                                                         cccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTc                                              <  2:3971446/101‑1 (MQ=255)
                                                          ccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCg                                             <  2:1219158/101‑1 (MQ=255)
                                                           cccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGt                                            <  1:1440744/101‑1 (MQ=255)
                                                           cccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGt                                            <  2:2176291/101‑1 (MQ=255)
                                                            ccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTg                                           >  1:936659/1‑101 (MQ=255)
                                                               cgGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGGAACGCGGCCGCGAGCAC.GTCGCTCCCCCCGCGGGGGGGTGGAACGAAACCGCTTTCGTGGGa                                        >  2:899959/1‑101 (MQ=38)
                                                                gggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGAc                                       >  1:3040025/1‑101 (MQ=255)
                                                                gggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGAc                                       <  1:1953724/101‑1 (MQ=255)
                                                                gggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGAc                                       >  1:3481857/1‑101 (MQ=255)
                                                                 ggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACt                                      <  2:56519/101‑1 (MQ=255)
                                                                    gCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGc                                   <  1:3104114/101‑1 (MQ=255)
                                                                     cGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGcc                                  >  2:1892628/1‑101 (MQ=255)
                                                                      gTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCa                                 >  2:1095347/1‑101 (MQ=255)
                                                                       tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAg                                <  2:3748515/101‑1 (MQ=255)
                                                                       tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAg                                >  2:3669357/1‑101 (MQ=255)
                                                                        ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGTCAgc                               <  2:2938570/101‑1 (MQ=255)
                                                                        ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgc                               <  2:2016854/101‑1 (MQ=255)
                                                                        ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgc                               >  2:3730966/1‑101 (MQ=255)
                                                                         gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgcc                              >  2:3863858/1‑101 (MQ=255)
                                                                         gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgcc                              >  2:127670/1‑101 (MQ=255)
                                                                            cGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGGCAgccgcc                           >  1:587087/1‑101 (MQ=255)
                                                                             gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCCTGGGACTGGCCACccggcc                          >  2:3170444/1‑101 (MQ=255)
                                                                              aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc.g                        >  2:1173472/1‑101 (MQ=255)
                                                                              aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc.g                        >  1:520350/1‑101 (MQ=255)
                                                                               aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAACCTGCTTTCGTGGGACTGGCCAGccgccc.gc                       >  1:280697/1‑101 (MQ=255)
                                                                               aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc.gc                       <  1:2597576/101‑1 (MQ=255)
                                                                                aCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCc.gcg                      >  2:475075/1‑101 (MQ=255)
                                                                                 cGGCCCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.gcgc                     <  2:1745843/101‑1 (MQ=255)
                                                                                    aCCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCaa                  >  2:3500584/1‑101 (MQ=255)
                                                                                    aCCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCaa                  <  2:3131673/101‑1 (MQ=255)
                                                                                      cTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGGAGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGt                >  2:3660477/1‑101 (MQ=255)
                                                                                       tGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTc               <  1:839496/101‑1 (MQ=255)
                                                                                         aaaTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGc             <  2:4062228/101‑1 (MQ=255)
                                                                                          aaTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCt            <  1:475132/101‑1 (MQ=255)
                                                                                           aTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTc           <  1:3911975/101‑1 (MQ=255)
                                                                                           aTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGCCGCTc           <  1:270410/101‑1 (MQ=255)
                                                                                            ttCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCAGCGccgacctcggc           <  1:789723/101‑10 (MQ=37)
                                                                                            ttCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTcc          >  2:363685/1‑101 (MQ=255)
                                                                                                   ggAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTGCCCCcgcg   >  2:3206959/1‑101 (MQ=255)
                                                                                                    gAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCgg  <  1:2861485/101‑1 (MQ=255)
                                                                                                    |                                                                                                      
GCGTGGATCGAAACCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGG  >  NC_014034/1396388‑1396588

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG