Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,500 C→T 6.5% R128* (CGA→TCA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,5000C→T 6.5% 24.9 ‑0.6 ‑1.6184R128* (CGA→TGA) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/5):  ref base (82/90):  total (89/95)
Fisher's exact test strand distribution p-value = 5.58e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.80e-02

Only 100 of 210 total aligned reads displayed.

CCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1396401‑1396599
                                                                                                   |                                                                                                     
 cgcgCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGa                                                                                                     <  1:2254453/101‑1 (MQ=255)
 cgcgCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGa                                                                                                     >  2:2764052/1‑101 (MQ=255)
   cgcCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGc                                                                                                   <  1:3449588/101‑1 (MQ=255)
    ggccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCa                                                                                                  <  1:4102034/99‑1 (MQ=255)
    gcCCTGCGGGTGATGCCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCa                                                                                                  <  1:1884279/101‑1 (MQ=255)
     cccTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAc                                                                                                 >  1:1745962/1‑101 (MQ=255)
        tGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTc                                                                                             <  2:1402369/101‑1 (MQ=255)
           gggTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCt                                                                                          <  1:2391393/101‑1 (MQ=255)
           gggTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCt                                                                                          >  2:2861266/1‑101 (MQ=255)
            ggTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTc                                                                                         >  1:2702747/1‑101 (MQ=255)
             gTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcc                                                                                        >  1:3997758/1‑101 (MQ=255)
              tGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTccc                                                                                       <  1:250219/101‑1 (MQ=255)
               gATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccc                                                                                      >  2:961341/1‑101 (MQ=255)
                aTGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTccccc                                                                                     <  2:2461445/101‑1 (MQ=255)
                aTGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTccccc                                                                                     <  2:3394229/101‑1 (MQ=255)
                 tGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTcccccc                                                                                    <  1:1901757/101‑1 (MQ=255)
                      aCGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCggg                                                                               <  1:1934901/101‑1 (MQ=255)
                       cGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCgggg                                                                              <  2:1197710/101‑1 (MQ=255)
                       cGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCgggg                                                                              >  1:2450056/1‑101 (MQ=255)
                          gCACCCCGGCGGTGGCTCCCCCCGCGGGGCCGGGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCg                                                                           <  1:1503990/101‑1 (MQ=39)
                          gCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCg                                                                           >  1:1546318/1‑101 (MQ=255)
                          gCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCg                                                                           <  1:1410566/101‑1 (MQ=255)
                           caccacGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGt                                                                          <  1:3910407/101‑1 (MQ=255)
                               acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTgggt                                                                      >  2:3176692/1‑99 (MQ=255)
                               acGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                      >  1:1351946/1‑101 (MQ=255)
                                cggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     <  1:1205707/101‑1 (MQ=255)
                                cggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                     >  2:871641/1‑101 (MQ=255)
                                 gtcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                    <  1:987948/99‑1 (MQ=255)
                                 gggggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                    <  1:100145/98‑1 (MQ=255)
                                 ggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                    <  1:2708836/101‑1 (MQ=255)
                                 ggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                    >  2:466607/1‑101 (MQ=255)
                                 ggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                    <  1:3685637/101‑1 (MQ=255)
                                 ggcggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGAGCg                                                                    >  2:3107921/1‑101 (MQ=255)
                                   cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                  >  2:2250159/1‑101 (MQ=255)
                                   cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                  <  1:2174213/101‑1 (MQ=255)
                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                >  1:3593058/1‑101 (MQ=255)
                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                               <  1:961432/101‑1 (MQ=255)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgct                                                             >  2:2645605/1‑98 (MQ=2)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctg                                                            <  2:777018/101‑5 (MQ=0)
                                         cTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctgg                                                           >  2:1551575/1‑96 (MQ=0)
                                          tCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACA..CCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgctggc                                                          <  1:3106840/101‑7 (MQ=0)
                                          cccccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCtt                                                           <  1:67555/100‑1 (MQ=255)
                                           ccccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCttt                                                          >  1:3967028/1‑101 (MQ=255)
                                             ccccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCg                                                        <  2:1219158/101‑1 (MQ=255)
                                              cccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGt                                                       <  2:2176291/101‑1 (MQ=255)
                                              cccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGt                                                       <  1:1440744/101‑1 (MQ=255)
                                              cccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGt                                                       <  1:2011050/101‑1 (MQ=255)
                                               ccGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTg                                                      >  1:936659/1‑101 (MQ=255)
                                                cgcgGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTgg                                                     >  1:1403750/1‑101 (MQ=255)
                                                  cgGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGGAACGCGGCCGCGAGCAC.GTCGCTCCCCCCGCGGGGGGGTGGAACGAAACCGCTTTCGTGGGa                                                   >  2:899959/1‑101 (MQ=38)
                                                  cgGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGa                                                   >  2:487677/1‑101 (MQ=255)
                                                  cgGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGa                                                   <  2:3959987/101‑1 (MQ=255)
                                                   gggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGAc                                                  >  1:3481857/1‑101 (MQ=255)
                                                     gggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTg                                                <  2:1149564/101‑1 (MQ=255)
                                                        cGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGcc                                             >  2:1892628/1‑101 (MQ=255)
                                                         gTGGATCTAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCa                                            <  1:709101/101‑1 (MQ=255)
                                                          tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAg                                           >  2:3669357/1‑101 (MQ=255)
                                                          tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAg                                           <  2:3748515/101‑1 (MQ=255)
                                                           ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGTCAgc                                          <  2:2938570/101‑1 (MQ=255)
                                                           ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgc                                          >  2:3730966/1‑101 (MQ=255)
                                                           ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgc                                          <  2:2016854/101‑1 (MQ=255)
                                                            gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgcc                                         >  2:3863858/1‑101 (MQ=255)
                                                            gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgcc                                         >  2:127670/1‑101 (MQ=255)
                                                            gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAgcc                                         >  1:1966673/1‑101 (MQ=255)
                                                               cGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGGCAgccgcc                                      >  1:587087/1‑101 (MQ=255)
                                                                gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GGCGCTCCCCCCGCGGGGGCGAGGACCGAAACTGCTTTCGTGGGGCTGGCTAGccgccc                                     >  1:3613982/1‑101 (MQ=38)
                                                                 aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc.g                                   >  1:520350/1‑101 (MQ=255)
                                                                  aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc.gc                                  <  2:2567817/101‑1 (MQ=255)
                                                                  aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc.gc                                  <  1:2597576/101‑1 (MQ=255)
                                                                  aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc.gc                                  >  1:3060254/1‑101 (MQ=255)
                                                                  aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGAAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGccgccc.gc                                  <  1:2950112/101‑1 (MQ=255)
                                                                    cGGCCCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.gcgc                                <  2:1745843/101‑1 (MQ=255)
                                                                    cGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.gcgc                                >  2:3236070/1‑101 (MQ=255)
                                                                       aCCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCaa                             <  2:3131673/101‑1 (MQ=255)
                                                                       aCCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCaa                             >  2:3500584/1‑101 (MQ=255)
                                                                         cTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGGAGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGt                           >  2:3660477/1‑101 (MQ=255)
                                                                         cTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGt                           >  1:408501/1‑101 (MQ=255)
                                                                         cTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGt                           >  1:2112474/1‑101 (MQ=255)
                                                                          tGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTc                          <  1:839496/101‑1 (MQ=255)
                                                                            aaaTTCCGCGGGAAACGCGGTGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGc                        >  1:1104376/1‑101 (MQ=255)
                                                                            aaaTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGc                        >  1:2022688/1‑101 (MQ=255)
                                                                             aaTTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCt                       <  1:475132/101‑1 (MQ=255)
                                                                               ttCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCCAGCGccgacctcggc                      <  1:789723/101‑10 (MQ=37)
                                                                               ttCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTcc                     >  2:363685/1‑101 (MQ=255)
                                                                                tCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTccc                    >  1:2366114/1‑101 (MQ=255)
                                                                                   gcgGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTcccccc                 <  1:1799430/101‑1 (MQ=255)
                                                                                       gagaCGCGGCGGCGAGCGA.GTCGCTCCCCCCGGGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCgg             <  2:1886236/98‑1 (MQ=38)
                                                                                       gAAACGCGGCGGCGGGCGC.GTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCTGTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCgg             <  2:3995428/101‑1 (MQ=38)
                                                                                       gAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCgg             <  2:2924059/101‑1 (MQ=255)
                                                                                       gAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCgg             <  1:2861485/101‑1 (MQ=255)
                                                                                        aaaCGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCggg            <  2:3149769/101‑1 (MQ=255)
                                                                                          aCGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCCAGTCGCTCCCCCCGCggggg          >  1:1679214/1‑101 (MQ=255)
                                                                                           cgcgGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGc         <  1:127694/101‑1 (MQ=255)
                                                                                           cgcgGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCCCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGc         >  2:1664402/1‑101 (MQ=255)
                                                                                             cggcggcgAGCAC.GTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGt       <  2:595327/101‑1 (MQ=255)
                                                                                              ggcggcgAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTg      >  2:147744/1‑101 (MQ=255)
                                                                                               gcggcgAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTgg     >  2:3904755/1‑101 (MQ=255)
                                                                                               gcggcgAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTgg     <  1:2562820/101‑1 (MQ=255)
                                                                                               gcggcgAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGGgg     >  2:199098/1‑101 (MQ=255)
                                                                                                 ggcgAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGAt   <  1:3236311/101‑1 (MQ=255)
                                                                                                   |                                                                                                     
CCGCGCCCTGCGGGTGATGGCGACGGGCACCACGGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCAC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTCGTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1396401‑1396599

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG