Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,551 A→G 15.2% T145A (ACT→GGC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,5510A→G15.2% 85.2 ‑2.2 ‑0.9237T145A (ACT→GCT) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (14/22):  ref base (108/93):  total (122/115)
Fisher's exact test strand distribution p-value = 1.07e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.34e-01

Only 100 of 253 total aligned reads displayed.

CGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCG  >  NC_014034/1396451‑1396651
                                                                                                     |                                                                                                     
cgGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGa                                                                                                       <  2:3959987/101‑1 (MQ=255)
 gggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGAc                                                                                                      >  1:3040025/1‑101 (MQ=255)
  ggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACt                                                                                                     <  2:56519/101‑1 (MQ=255)
   gggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTg                                                                                                    <  2:1149564/101‑1 (MQ=255)
     gCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGc                                                                                                  <  1:3104114/101‑1 (MQ=255)
       gTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCa                                                                                                >  2:1095347/1‑101 (MQ=255)
       gTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCa                                                                                                <  2:893189/101‑1 (MQ=255)
        tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAg                                                                                               >  2:3669357/1‑101 (MQ=255)
         ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGTCAgc                                                                                              <  2:2938570/101‑1 (MQ=255)
         ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgc                                                                                              >  2:3730966/1‑101 (MQ=255)
          gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgcc                                                                                             >  2:127670/1‑101 (MQ=255)
             cGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGGCAgccgcc                                                                                          >  1:587087/1‑101 (MQ=255)
              gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.CTGGGACTGGCCACccggcc                                                                                         >  2:3170444/1‑101 (MQ=255)
                aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgccc.gc                                                                                      <  2:2567817/101‑1 (MQ=255)
                 aCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCc.gcg                                                                                     >  1:2879154/1‑101 (MQ=255)
                  cGGACCTGAAATTCCGCGGGAAACTCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.gcgc                                                                                    <  1:2574129/101‑1 (MQ=255)
                  cGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.gcgc                                                                                    >  2:3236070/1‑101 (MQ=255)
                    gACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCa                                                                                  <  1:950825/101‑1 (MQ=255)
                          aaaTTCCGCGGGAAACGCGGTGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGc                                                                            >  1:1104376/1‑101 (MQ=255)
                          aaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGc                                                                            >  1:2022688/1‑101 (MQ=255)
                          aaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGc                                                                            <  2:4062228/101‑1 (MQ=255)
                            aTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTc                                                                          >  1:1764399/1‑101 (MQ=255)
                             ttCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCAGCGccgacctcggc                                                                          <  1:789723/101‑10 (MQ=37)
                             ttCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTcc                                                                         >  2:363685/1‑101 (MQ=255)
                                 gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTcccccc                                                                     <  1:1641167/101‑1 (MQ=255)
                                     gAAACGCGGCGGCGGGCGCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCTGTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCgg                                                                 <  2:3995428/101‑1 (MQ=38)
                                       aaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCgggg                                                               <  1:1376638/101‑1 (MQ=255)
                                        agctggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCggggg                                                              >  2:3140138/5‑101 (MQ=255)
                                         cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGc                                                             <  1:1892748/101‑1 (MQ=255)
                                         cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGc                                                             <  1:127694/101‑1 (MQ=255)
                                         cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCCCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGc                                                             >  2:1664402/1‑101 (MQ=255)
                                           cggcggcgAGCACGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGt                                                           <  2:595327/101‑1 (MQ=255)
                                              cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                        >  2:3887248/1‑101 (MQ=255)
                                              cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                        >  1:1921959/1‑101 (MQ=255)
                                              cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                        <  1:3500837/101‑1 (MQ=255)
                                              cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                        <  2:1408839/101‑1 (MQ=255)
                                               ggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                       <  1:3236311/101‑1 (MQ=255)
                                               ggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGACCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                       >  1:2118572/1‑101 (MQ=255)
                                                gggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGAt                                                       <  2:3074343/93‑1 (MQ=2)
                                                gcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                      <  1:3373078/101‑1 (MQ=255)
                                                 ggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATc                                                      >  1:710791/8‑101 (MQ=2)
                                                  gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                                     <  2:777493/95‑1 (MQ=2)
                                                  gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                                     >  2:1770830/7‑101 (MQ=2)
                                                  gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                                     <  1:1596964/95‑1 (MQ=2)
                                                  gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                                     <  1:63788/95‑1 (MQ=2)
                                                  gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                    >  2:2307687/1‑101 (MQ=255)
                                                  gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                    >  1:3479219/1‑101 (MQ=255)
                                                   acggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGa                                                    <  2:1998846/96‑1 (MQ=2)
                                                   acggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGa                                                    >  2:1872176/6‑101 (MQ=2)
                                                   acggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGa                                                    <  1:776596/96‑1 (MQ=2)
                                                    gCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  <  2:1518126/101‑1 (MQ=255)
                                                    gCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCCCCCCCCGCGGGGGCGTGGGTCGaaa                                                  >  1:2124528/1‑101 (MQ=255)
                                                     cACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                 >  1:2625086/1‑101 (MQ=255)
                                                     cACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                 <  1:3176931/101‑1 (MQ=255)
                                                        gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGagatcggaag                                               >  1:46186/1‑92 (MQ=2)
                                                        gTCGCTCCCACCGCGGGGGCGCGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCCCCC.GCGCCAAGTCGCTCCCCCCCCGTGGGCGTGGATCGACACGac                                              >  2:477429/1‑100 (MQ=38)
                                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                            <  2:936574/101‑1 (MQ=255)
                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                           >  1:1740208/1‑101 (MQ=255)
                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                           <  2:2632148/101‑1 (MQ=255)
                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                           >  2:2374115/1‑101 (MQ=255)
                                                            cTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcg                                          <  1:2815292/101‑1 (MQ=255)
                                                            cTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcg                                          >  2:2198729/1‑101 (MQ=255)
                                                            cTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcg                                          <  1:2764271/101‑1 (MQ=255)
                                                            cTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGCACTGGCCAGCCCCCC.GCGCCAAGTCGCTCCCCCCGCCGGGGCGCCGATCGAAACGATTtcg                                          >  1:973087/1‑101 (MQ=37)
                                                            cTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgcc                                           <  1:361102/101‑7 (MQ=2)
                                                             cccccccGCGGGGGCGGGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                         <  1:1505668/100‑1 (MQ=255)
                                                               cccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtcg                                       >  2:156160/1‑101 (MQ=255)
                                                               cccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                        >  2:3922592/1‑92 (MQ=2)
                                                               cccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                        <  1:2389571/101‑10 (MQ=2)
                                                                 cccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCt                                     <  1:4121689/101‑1 (MQ=255)
                                                                  ccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCtt                                    <  1:509974/101‑1 (MQ=255)
                                                                   cgcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                                   <  2:1021764/101‑1 (MQ=255)
                                                                    gcgGGGGTGTGGATCGAAACTGCTTTC.GTGGGAGTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                  <  1:3665318/101‑1 (MQ=255)
                                                                    gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                  <  2:4207517/101‑1 (MQ=255)
                                                                     cgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacg                                 <  2:3039800/101‑1 (MQ=255)
                                                                      gggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacga                                <  2:3592805/101‑1 (MQ=255)
                                                                      gggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacga                                >  2:3410091/1‑101 (MQ=255)
                                                                       ggtgCGTGGAGCGAAACTGCTGTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                               <  1:900044/98‑1 (MQ=255)
                                                                       ggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                               >  1:1037435/1‑101 (MQ=255)
                                                                       ggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                               >  2:3861487/1‑101 (MQ=255)
                                                                           cGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCt                           >  2:441162/1‑101 (MQ=255)
                                                                             tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                         <  1:2250318/101‑1 (MQ=255)
                                                                             tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                         >  2:2866427/1‑101 (MQ=255)
                                                                             tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                         >  2:2488559/1‑101 (MQ=255)
                                                                              ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                        >  1:11561/1‑101 (MQ=255)
                                                                              ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACGATTTAGCCGCTTACGACGCCTATc                        >  1:3810044/1‑101 (MQ=255)
                                                                               gATCGAAACTGGTTAC.GTGGGACTGGCCGGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt                       <  2:3481605/101‑1 (MQ=255)
                                                                                aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                      >  1:3083165/1‑101 (MQ=255)
                                                                                 tCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGa                     >  2:4135038/1‑101 (MQ=255)
                                                                                   gAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt                   >  1:4056389/1‑101 (MQ=255)
                                                                                    aaaCTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                  >  1:3828199/1‑101 (MQ=255)
                                                                                    aaaCTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                  <  1:1966419/101‑1 (MQ=255)
                                                                                       cTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCgg               <  2:2120533/101‑1 (MQ=255)
                                                                                         gCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGt             >  1:2461570/1‑101 (MQ=255)
                                                                                          cTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTTCGACGCCTATCTGATTTCGGGTc            >  2:1356501/1‑101 (MQ=255)
                                                                                            ttC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGc          >  2:3119607/1‑101 (MQ=255)
                                                                                              c.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc        >  1:432826/1‑101 (MQ=255)
                                                                                                gTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc       >  2:1912821/1‑101 (MQ=255)
                                                                                                gTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc       >  2:3785045/1‑101 (MQ=255)
                                                                                                     aCTGGCCCGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcg  <  2:1679099/101‑1 (MQ=255)
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CGGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCG  >  NC_014034/1396451‑1396651

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG