Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,552 C→G 15.6% T145S (ACT→GGC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,5520C→G15.6% 87.5 ‑2.6 ‑0.0237T145S (ACT→AGT) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (14/23):  ref base (108/92):  total (122/115)
Fisher's exact test strand distribution p-value = 7.62e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.98e-01

Only 100 of 252 total aligned reads displayed.

GGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGC  >  NC_014034/1396452‑1396652
                                                                                                     |                                                                                                    
gggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGAc                                                                                                      >  1:3481857/1‑101 (MQ=255)
 ggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACt                                                                                                     <  2:56519/101‑1 (MQ=255)
      gTGGATCTAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCa                                                                                                <  1:709101/101‑1 (MQ=255)
      gTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCa                                                                                                >  2:3824226/1‑101 (MQ=255)
       tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAg                                                                                               >  2:3669357/1‑101 (MQ=255)
        ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGTCAgc                                                                                              <  2:2938570/101‑1 (MQ=255)
        ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgc                                                                                              <  2:2016854/101‑1 (MQ=255)
        ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgc                                                                                              >  2:3730966/1‑101 (MQ=255)
        ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgc                                                                                              >  1:2849742/1‑101 (MQ=255)
          aTCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgccg                                                                                            >  1:781544/1‑101 (MQ=255)
             gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.CTGGGACTGGCCACccggcc                                                                                         >  2:3170444/1‑101 (MQ=255)
              aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgcccg                                                                                        >  2:1173472/1‑101 (MQ=255)
              aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgcccg                                                                                        >  1:520350/1‑101 (MQ=255)
               aaCGGACCTGGAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCAAAACTGCTTTC.GTGGGACGGGCCAGccgccccc                                                                                       >  1:3654518/1‑99 (MQ=255)
               aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCTGCTTTC.GTGGGACTGGCCAGccgcccgc                                                                                       >  1:280697/1‑101 (MQ=255)
               aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgcccgc                                                                                       <  1:2597576/101‑1 (MQ=255)
               aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGAACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgcccgc                                                                                       <  1:2950112/101‑1 (MQ=255)
                 cGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCgcgc                                                                                     >  2:3236070/1‑101 (MQ=255)
                  ggACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGcc                                                                                    >  2:93132/1‑101 (MQ=255)
                      cTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGt                                                                                >  1:408501/1‑101 (MQ=255)
                       tGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTc                                                                               <  1:839496/101‑1 (MQ=255)
                         aaaTTCCGCGGGAAACGCGGTGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGc                                                                             >  1:1104376/1‑101 (MQ=255)
                         aaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGc                                                                             >  1:2022688/1‑101 (MQ=255)
                         aaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGc                                                                             <  2:4062228/101‑1 (MQ=255)
                          aaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCt                                                                            <  1:475132/101‑1 (MQ=255)
                           aTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTc                                                                           >  1:1764399/1‑101 (MQ=255)
                           aTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTc                                                                           <  1:3911975/101‑1 (MQ=255)
                           aTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGCCGCTc                                                                           <  1:270410/101‑1 (MQ=255)
                            ttCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcc                                                                          >  2:363685/1‑101 (MQ=255)
                             tCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTccc                                                                         >  1:2366114/1‑101 (MQ=255)
                                gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccccc                                                                      <  1:1641167/101‑1 (MQ=255)
                                    gagaCGCGGCGGCGAGCGAGTCGCTCCCCCCGGGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgg                                                                  <  2:1886236/98‑1 (MQ=38)
                                    gAAACGCGGCGGCGGGCGCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCTGTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgg                                                                  <  2:3995428/101‑1 (MQ=38)
                                      aaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgggg                                                                <  1:1376638/101‑1 (MQ=255)
                                       agctggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCggggg                                                               >  2:3140138/5‑101 (MQ=255)
                                        cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGc                                                              <  1:1892748/101‑1 (MQ=255)
                                        cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGc                                                              <  1:127694/101‑1 (MQ=255)
                                            gcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                          <  2:2702541/101‑1 (MQ=255)
                                            gcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                          <  1:593468/101‑1 (MQ=255)
                                            gcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGGgg                                                          >  2:199098/1‑101 (MQ=255)
                                             cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                         >  1:1921959/1‑101 (MQ=255)
                                             cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                         >  2:3887248/1‑101 (MQ=255)
                                              ggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGACCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                        >  1:2118572/1‑101 (MQ=255)
                                              gcctcgCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCTGCTTTC.GTGGGACTCGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                        <  2:433268/96‑1 (MQ=255)
                                               gggacggggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGAt                                                        <  1:3070347/93‑1 (MQ=2)
                                               gggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGAt                                                        <  2:3074343/93‑1 (MQ=2)
                                                ggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATc                                                       <  2:1531513/94‑1 (MQ=2)
                                                 gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                                      <  1:1596964/95‑1 (MQ=2)
                                                 gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                                      <  2:777493/95‑1 (MQ=2)
                                                 gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                     >  2:2307687/1‑101 (MQ=255)
                                                  gcggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                     <  2:2557425/96‑1 (MQ=2)
                                                  aGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                    >  2:1671555/1‑101 (MQ=255)
                                                   gCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCCCCCCCCGCGGGGGCGTGGGTCGaaa                                                   >  1:2124528/1‑101 (MQ=255)
                                                    ggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGaaa                                                   <  2:12812/98‑1 (MQ=2)
                                                      agTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAAct                                                 <  1:3098174/100‑2 (MQ=2)
                                                      agTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGGGGATCGAAAct                                                 >  2:1708598/2‑100 (MQ=2)
                                                       gTCGCTCCCACCGCGGGGGCGCGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCCCCCGCGCCAAGTCGCTCCCCCCCCGTGGGCGTGGATCGACACGac                                               >  2:477429/1‑100 (MQ=38)
                                                        tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAtt                                              >  1:3511283/1‑101 (MQ=255)
                                                         cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                              <  1:4122007/101‑5 (MQ=2)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                            >  1:2542809/1‑101 (MQ=255)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                            <  2:2632148/101‑1 (MQ=255)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                            >  2:2374115/1‑101 (MQ=255)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                            >  1:1740208/1‑101 (MQ=255)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                            <  2:3966768/101‑1 (MQ=255)
                                                            tCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                          >  2:609393/1‑101 (MQ=255)
                                                            cccccccGCGGGGGCGGGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                          <  1:1505668/100‑1 (MQ=255)
                                                             ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                         <  1:93156/101‑1 (MQ=255)
                                                             ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                         >  2:4121428/1‑101 (MQ=255)
                                                              cccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtcg                                        <  1:745205/101‑1 (MQ=255)
                                                              cccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                         >  2:3029435/1‑92 (MQ=2)
                                                               ccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTAGc                                       >  2:3921989/1‑101 (MQ=255)
                                                               ccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggtt                                        <  1:381193/101‑11 (MQ=2)
                                                                 ccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCtt                                     >  2:1607721/1‑101 (MQ=255)
                                                                  cgggggggCGTGGATCGAAACTGCTTTC.GTGCGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                                    <  1:993292/98‑1 (MQ=255)
                                                                  cgcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                                    >  1:964675/1‑101 (MQ=255)
                                                                   gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                   <  2:4207517/101‑1 (MQ=255)
                                                                   gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTACCCCCGCGGGGGCGAGGATAGAAAAAAATTCGTCGCTTac                                   >  1:445185/1‑101 (MQ=37)
                                                                     gggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacga                                 <  2:3592805/101‑1 (MQ=255)
                                                                     gggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacga                                 >  2:3410091/1‑101 (MQ=255)
                                                                      ggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                                >  1:1037435/1‑101 (MQ=255)
                                                                      ggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                                <  1:3669610/101‑1 (MQ=255)
                                                                         gCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGcc                             <  1:3410336/101‑1 (MQ=255)
                                                                           gTGGATCGAAACTGCTTTC.GTGGGACTGGCCCGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                           <  1:3207198/101‑1 (MQ=255)
                                                                            tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                          <  1:2250318/101‑1 (MQ=255)
                                                                            tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                          >  1:3142320/1‑101 (MQ=255)
                                                                            tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                          >  2:1063163/1‑101 (MQ=255)
                                                                            tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                          >  2:2488559/1‑101 (MQ=255)
                                                                             ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                         >  2:2229406/1‑101 (MQ=255)
                                                                             ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                         >  1:11561/1‑101 (MQ=255)
                                                                              gATCGAAACTGGTTAC.GTGGGACTGGCCGGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt                        <  2:3481605/101‑1 (MQ=255)
                                                                               aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                       <  2:1104282/101‑1 (MQ=255)
                                                                                   aaaCTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                   <  1:1966419/101‑1 (MQ=255)
                                                                                     aCTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCg                 <  2:1418481/101‑1 (MQ=255)
                                                                                       tGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCggg               >  1:3304975/1‑101 (MQ=255)
                                                                                        gCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGt              >  1:2461570/1‑101 (MQ=255)
                                                                                           ttC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGc           >  2:3119607/1‑101 (MQ=255)
                                                                                            tC.GGGGGACTGGCCGGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCt          <  2:2197882/101‑1 (MQ=255)
                                                                                               gTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc        >  2:3785045/1‑101 (MQ=255)
                                                                                                tGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTccc       <  2:1403651/101‑1 (MQ=255)
                                                                                                tGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTccc       >  2:1816309/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
GGGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGC  >  NC_014034/1396452‑1396652

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG