Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,553 T→C 15.1% T145T (ACT→GGC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,5530T→C15.1% 78.8 ‑2.2 ‑0.0237T145T (ACT→ACC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (14/22):  ref base (108/92):  total (123/114)
Fisher's exact test strand distribution p-value = 1.06e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.75e-01

Only 100 of 251 total aligned reads displayed.

GGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCG  >  NC_014034/1396453‑1396653
                                                                                                     |                                                                                                     
ggggCGTGGATCGAAACGGACCTGAAATTCCTCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACt                                                                                                       <  2:2361109/101‑1 (MQ=255)
ggggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACt                                                                                                       <  2:56519/101‑1 (MQ=255)
 gggCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTg                                                                                                      <  2:1149564/101‑1 (MQ=255)
   gCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGc                                                                                                    <  1:3104114/101‑1 (MQ=255)
     gTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCa                                                                                                  >  2:1095347/1‑101 (MQ=255)
       ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgc                                                                                                <  2:3727252/101‑1 (MQ=255)
       ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgc                                                                                                >  1:2849742/1‑101 (MQ=255)
        gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgcc                                                                                               >  2:127670/1‑101 (MQ=255)
        gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgcc                                                                                               >  1:1966673/1‑101 (MQ=255)
           cGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGGCAgccgcc                                                                                            >  1:587087/1‑101 (MQ=255)
            gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGGCGCTCCCCCCGCGGGGGCGAGGACCGAAACTGCTTTC.GTGGGGCTGGCTAGccgccc                                                                                           >  1:3613982/1‑101 (MQ=38)
              aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCTGCTTTC.GTGGGACTGGCCAGccgccc.gc                                                                                        >  1:280697/1‑101 (MQ=255)
              aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgccc.gc                                                                                        <  1:2597576/101‑1 (MQ=255)
              aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGAACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgccc.gc                                                                                        <  1:2950112/101‑1 (MQ=255)
                cGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.gcgc                                                                                      >  2:3733573/1‑101 (MQ=255)
                 ggACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGcc                                                                                     >  2:93132/1‑101 (MQ=255)
                  gACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCa                                                                                    <  1:950825/101‑1 (MQ=255)
                   aCCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCaa                                                                                   >  2:3500584/1‑101 (MQ=255)
                   aCCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCaa                                                                                   <  2:3131673/101‑1 (MQ=255)
                     cTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGGAGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGt                                                                                 >  2:3660477/1‑101 (MQ=255)
                     cTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGt                                                                                 >  2:2519203/1‑101 (MQ=255)
                        aaaTTCCGCGGGAAACGCGGTGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGc                                                                              >  1:1104376/1‑101 (MQ=255)
                        aaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGc                                                                              >  1:2022688/1‑101 (MQ=255)
                        aaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGc                                                                              <  2:4062228/101‑1 (MQ=255)
                          aTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTc                                                                            >  1:1764399/1‑101 (MQ=255)
                          aTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGCCGCTc                                                                            <  1:270410/101‑1 (MQ=255)
                           ttCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCAGCGccgacctcggc                                                                            <  1:789723/101‑10 (MQ=37)
                            tCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTccc                                                                          >  1:2366114/1‑101 (MQ=255)
                               gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTcccccc                                                                       <  1:1641167/101‑1 (MQ=255)
                                cgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCcg                                                                      <  2:2439207/101‑1 (MQ=255)
                                  ggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTGCCCCcgcg                                                                    >  2:3206959/1‑101 (MQ=255)
                                   gAAACGCGGCGGCGGGCGCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCTGTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCgg                                                                   <  2:3995428/101‑1 (MQ=38)
                                   gAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCgg                                                                   <  2:2924059/101‑1 (MQ=255)
                                    aaaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCggg                                                                  <  2:3149769/101‑1 (MQ=255)
                                     aaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCgggg                                                                 <  1:1376638/101‑1 (MQ=255)
                                       cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGc                                                               <  1:127694/101‑1 (MQ=255)
                                       cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGc                                                               <  1:1892748/101‑1 (MQ=255)
                                       cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCCCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGc                                                               >  2:1664402/1‑101 (MQ=255)
                                            cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                          <  1:3500837/101‑1 (MQ=255)
                                            cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                          >  2:3887248/1‑101 (MQ=255)
                                            cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                          <  2:1408839/101‑1 (MQ=255)
                                            cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                          >  1:1921959/1‑101 (MQ=255)
                                             ggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         <  1:3236311/101‑1 (MQ=255)
                                             ggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGACCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         >  1:2118572/1‑101 (MQ=255)
                                             gcctcgCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCTGCTTTC.GTGGGACTCGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         <  2:433268/96‑1 (MQ=255)
                                              gggacggggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGAt                                                         <  1:3070347/93‑1 (MQ=2)
                                               ggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATc                                                        >  1:710791/8‑101 (MQ=2)
                                                gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                                       <  2:777493/95‑1 (MQ=2)
                                                gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                                       >  2:1770830/7‑101 (MQ=2)
                                                gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                                       <  1:1596964/95‑1 (MQ=2)
                                                gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                                       <  1:63788/95‑1 (MQ=2)
                                                gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                      >  1:3479219/1‑101 (MQ=255)
                                                 acggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGa                                                      <  2:1998846/96‑1 (MQ=2)
                                                 acggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGa                                                      >  2:1872176/6‑101 (MQ=2)
                                                 acggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGa                                                      <  1:776596/96‑1 (MQ=2)
                                                  gCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    <  2:1518126/101‑1 (MQ=255)
                                                  gCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCCCCCCCCGCGGGGGCGTGGGTCGaaa                                                    >  1:2124528/1‑101 (MQ=255)
                                                     cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGTCT.CCGGGCAGTCGCCTCCCCCGCCGGGGCGCGGTTCGAAAAGa                                                 >  2:3791587/1‑101 (MQ=18)
                                                      ggcGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                <  2:2652742/99‑1 (MQ=255)
                                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGagatcggaag                                                 >  1:46186/1‑92 (MQ=2)
                                                      gTCGCTCCCACCGCGGGGGCGCGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCCCCC.GCGCCAAGTCGCTCCCCCCCCGTGGGCGTGGATCGACACGac                                                >  2:477429/1‑100 (MQ=38)
                                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAtt                                               <  2:3252071/101‑1 (MQ=255)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATAGatcggaaga                                              >  2:3107064/1‑93 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                             >  1:1740208/1‑101 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                             <  2:3966768/101‑1 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                             >  1:2542809/1‑101 (MQ=255)
                                                          cTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcg                                            >  2:2198729/1‑101 (MQ=255)
                                                          cTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGCACTGGCCAGCCCCCC.GCGCCAAGTCGCTCCCCCCGCCGGGGCGCCGATCGAAACGATTtcg                                            >  1:973087/1‑101 (MQ=37)
                                                           tCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                           >  2:609393/1‑101 (MQ=255)
                                                           cccccccGCGGGGGCGGGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                           <  1:1505668/100‑1 (MQ=255)
                                                            ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                          >  2:4121428/1‑101 (MQ=255)
                                                             cccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtcg                                         >  2:156160/1‑101 (MQ=255)
                                                              ccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTAGc                                        >  2:3921989/1‑101 (MQ=255)
                                                               cccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCt                                       <  1:4121689/101‑1 (MQ=255)
                                                                ccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCtt                                      <  1:509974/101‑1 (MQ=255)
                                                                 cgcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                                     <  2:1021764/101‑1 (MQ=255)
                                                                  gcgGGGGTGTGGATCGAAACTGCTTTC.GTGGGAGTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                    <  1:3665318/101‑1 (MQ=255)
                                                                  gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                    <  2:4207517/101‑1 (MQ=255)
                                                                     ggtgCGTGGAGCGAAACTGCTGTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                                 <  1:900044/98‑1 (MQ=255)
                                                                     ggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                                 >  1:1037435/1‑101 (MQ=255)
                                                                         cGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCt                             >  2:3434577/1‑101 (MQ=255)
                                                                           tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                           <  1:2250318/101‑1 (MQ=255)
                                                                           tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                           >  1:3142320/1‑101 (MQ=255)
                                                                            ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                          >  1:11561/1‑101 (MQ=255)
                                                                            ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACGATTTAGCCGCTTACGACGCCTATc                          >  1:3810044/1‑101 (MQ=255)
                                                                             gATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGATTTCGTCCCCTACGACGCCTCTCt                         >  2:3605997/1‑101 (MQ=38)
                                                                                cGAAACTGCTTTC.GTGGGACTGGCCAGCAGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAt                      >  2:3056537/1‑101 (MQ=255)
                                                                                 gAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt                     >  1:4056389/1‑101 (MQ=255)
                                                                                  aaaCTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                    >  1:3828199/1‑101 (MQ=255)
                                                                                     cTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCgg                 <  2:2120533/101‑1 (MQ=255)
                                                                                      tGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCggg                >  1:3304975/1‑101 (MQ=255)
                                                                                       gCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGt               >  1:2461570/1‑101 (MQ=255)
                                                                                        cTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTTCGACGCCTATCTGATTTCGGGTc              >  2:1356501/1‑101 (MQ=255)
                                                                                          ttC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGc            >  2:2605145/1‑101 (MQ=255)
                                                                                            c.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc          >  1:432826/1‑101 (MQ=255)
                                                                                              gTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc         >  2:1912821/1‑101 (MQ=255)
                                                                                                 ggACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTccccc      <  2:29753/101‑1 (MQ=255)
                                                                                                   aCTGGCCCGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcg    <  2:1679099/101‑1 (MQ=255)
                                                                                                    cTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgc   >  2:2293873/1‑101 (MQ=255)
                                                                                                     tGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgcg  >  2:3830573/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
GGGGCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCG  >  NC_014034/1396453‑1396653

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG