Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,556 C→G 15.1% G146G (GGC→GGG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,5560C→G15.1% 87.9 ‑2.3 ‑0.0240G146G (GGC→GGG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (14/22):  ref base (112/91):  total (126/114)
Fisher's exact test strand distribution p-value = 1.02e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.47e-01

Only 100 of 256 total aligned reads displayed.

GCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGG  >  NC_014034/1396456‑1396656
                                                                                                     |                                                                                                    
gCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGc                                                                                                      <  1:3104114/101‑1 (MQ=255)
  gTGGATCTAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCa                                                                                                    <  1:709101/101‑1 (MQ=255)
   tGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAg                                                                                                   >  2:3669357/1‑101 (MQ=255)
    ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGTCAgc                                                                                                  <  2:2938570/101‑1 (MQ=255)
    ggATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgc                                                                                                  >  1:2849742/1‑101 (MQ=255)
     gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgcc                                                                                                 >  2:127670/1‑101 (MQ=255)
     gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgcc                                                                                                 >  2:3863858/1‑101 (MQ=255)
     gATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgcc                                                                                                 >  1:1966673/1‑101 (MQ=255)
      aTCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAgccg                                                                                                >  1:781544/1‑101 (MQ=255)
         gAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.CTGGGACTGGCCACccggcc                                                                                             >  2:3170444/1‑101 (MQ=255)
           aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCTGCTTTC.GTGGGACTGGCCAGccgcccgc                                                                                           >  1:280697/1‑101 (MQ=255)
           aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGAACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgcccgc                                                                                           <  1:2950112/101‑1 (MQ=255)
             cGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCgcgc                                                                                         >  2:3236070/1‑101 (MQ=255)
             cGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCgcgc                                                                                         >  2:3733573/1‑101 (MQ=255)
              ggACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGcc                                                                                        >  2:93132/1‑101 (MQ=255)
                  cTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGt                                                                                    >  2:2519203/1‑101 (MQ=255)
                   tGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTc                                                                                   <  1:839496/101‑1 (MQ=255)
                     aaaTTCCGCGGGAAACGCGGTGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGc                                                                                 >  1:1104376/1‑101 (MQ=255)
                      aaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCt                                                                                <  1:475132/101‑1 (MQ=255)
                       aTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTc                                                                               <  1:3911975/101‑1 (MQ=255)
                        ttCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcc                                                                              >  2:363685/1‑101 (MQ=255)
                            gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccccc                                                                          <  1:1799430/101‑1 (MQ=255)
                            gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTcccccc                                                                          <  1:1641167/101‑1 (MQ=255)
                             cgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCcg                                                                         <  2:2439207/101‑1 (MQ=255)
                                gAAACGCGGCGGCGGGCGCGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTGCTGTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgg                                                                      <  2:3995428/101‑1 (MQ=38)
                                  aaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCgggg                                                                    <  1:1376638/101‑1 (MQ=255)
                                   agctggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCggggg                                                                   >  2:3140138/5‑101 (MQ=255)
                                    cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGc                                                                  <  1:1892748/101‑1 (MQ=255)
                                    cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGc                                                                  <  1:127694/101‑1 (MQ=255)
                                       ggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTg                                                               >  2:147744/1‑101 (MQ=255)
                                        gcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                              <  1:2562820/101‑1 (MQ=255)
                                        gcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                              <  1:593468/101‑1 (MQ=255)
                                        gcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                              <  2:2702541/101‑1 (MQ=255)
                                         cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                             <  2:1408839/101‑1 (MQ=255)
                                         cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                             <  1:3500837/101‑1 (MQ=255)
                                          ggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGACCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                            >  1:2118572/1‑101 (MQ=255)
                                          gcctcgCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCTGCTTTC.GTGGGACTCGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                            <  2:433268/96‑1 (MQ=255)
                                           gggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGAt                                                            <  2:3074343/93‑1 (MQ=2)
                                             gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                                          <  1:1596964/95‑1 (MQ=2)
                                             gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                                          >  2:1770830/7‑101 (MQ=2)
                                             gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                         >  2:2307687/1‑101 (MQ=255)
                                              gcggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                         <  2:2557425/96‑1 (MQ=2)
                                              acggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGa                                                         <  2:1998846/96‑1 (MQ=2)
                                              acggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGa                                                         >  2:1872176/6‑101 (MQ=2)
                                               gCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                       <  2:4044722/101‑1 (MQ=255)
                                                cACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                      >  1:2625086/1‑101 (MQ=255)
                                                cACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                      <  1:3176931/101‑1 (MQ=255)
                                                   gTCGCTCCCACCGCGGGGGCGCGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCCCCCGCGCCAAGTCGCTCCCCCCCCGTGGGCGTGGATCGACACGac                                                   >  2:477429/1‑100 (MQ=38)
                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAttt                                                 <  2:131230/101‑1 (MQ=255)
                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                                  >  2:566769/1‑97 (MQ=2)
                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                                  <  1:4122007/101‑5 (MQ=2)
                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                                <  2:2632148/101‑1 (MQ=255)
                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                                >  1:1740208/1‑101 (MQ=255)
                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgc                                                 >  2:164189/1‑96 (MQ=2)
                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcg                                               >  2:2198729/1‑101 (MQ=255)
                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgcc                                                <  1:361102/101‑7 (MQ=2)
                                                        cccccccGCGGGGGCGGGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                              <  1:1505668/100‑1 (MQ=255)
                                                         ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                             >  2:4121428/1‑101 (MQ=255)
                                                         ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                             <  1:93156/101‑1 (MQ=255)
                                                          cccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtcg                                            >  2:3949492/1‑101 (MQ=255)
                                                          cccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtcg                                            >  2:156160/1‑101 (MQ=255)
                                                          cccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtcg                                            <  1:745205/101‑1 (MQ=255)
                                                          cccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                             >  2:3029435/1‑92 (MQ=2)
                                                          cccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                             <  1:2389571/101‑10 (MQ=2)
                                                           ccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTAGc                                           >  2:3921989/1‑101 (MQ=255)
                                                           ccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCAACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggtt                                            <  1:381193/101‑11 (MQ=2)
                                                             ccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCtt                                         <  1:509974/101‑1 (MQ=255)
                                                              cgggggggCGTGGATCGAAACTGCTTTC.GTGCGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                                        <  1:993292/98‑1 (MQ=255)
                                                              cgcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                                        >  1:964675/1‑101 (MQ=255)
                                                               gcgGGGGTGTGGATCGAAACTGCTTTC.GTGGGAGTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                       <  1:3665318/101‑1 (MQ=255)
                                                               gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                       <  2:4207517/101‑1 (MQ=255)
                                                                 gggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacga                                     <  2:3592805/101‑1 (MQ=255)
                                                                 gggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacga                                     >  2:3410091/1‑101 (MQ=255)
                                                                  ggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                                    >  1:1037435/1‑101 (MQ=255)
                                                                     gCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGcc                                 <  1:3410336/101‑1 (MQ=255)
                                                                        tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                              <  1:1954833/101‑1 (MQ=255)
                                                                        tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                              <  1:2250318/101‑1 (MQ=255)
                                                                        tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                              >  2:1063163/1‑101 (MQ=255)
                                                                         ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                             >  1:11561/1‑101 (MQ=255)
                                                                          gATCGAAACTGGTTAC.GTGGGACTGGCCGGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt                            <  2:3481605/101‑1 (MQ=255)
                                                                           aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                           >  1:3083165/1‑101 (MQ=255)
                                                                           aTCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTg                           <  2:1104282/101‑1 (MQ=255)
                                                                             cGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAt                         >  1:2053316/1‑101 (MQ=255)
                                                                              gAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt                        >  1:4056389/1‑101 (MQ=255)
                                                                                  cTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCgg                    <  2:2120533/101‑1 (MQ=255)
                                                                                   tGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCggg                   >  1:3304975/1‑101 (MQ=255)
                                                                                       ttC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGc               >  2:3119607/1‑101 (MQ=255)
                                                                                         c.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTc             >  1:432826/1‑101 (MQ=255)
                                                                                           gTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc            >  2:3785045/1‑101 (MQ=255)
                                                                                           gTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc            >  2:1912821/1‑101 (MQ=255)
                                                                                             gggACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcccc          >  2:648892/1‑101 (MQ=255)
                                                                                                gcTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcg       <  1:2488743/100‑1 (MQ=255)
                                                                                                aCTGGCCCGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcg       <  2:1679099/101‑1 (MQ=255)
                                                                                                aCTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcg       >  1:2151634/1‑101 (MQ=255)
                                                                                                  tGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgcg     >  2:3830573/1‑101 (MQ=255)
                                                                                                    gCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCggg   >  1:3209246/1‑101 (MQ=255)
                                                                                                    gCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCggg   <  2:781466/101‑1 (MQ=255)
                                                                                                    gCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCggg   <  2:2252992/101‑1 (MQ=255)
                                                                                                    gCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGAACGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCggg   >  1:2734862/1‑101 (MQ=255)
                                                                                                     ccAGCCGCCCGCGCCAAGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGATCCCCCCGCgggg  <  1:3805148/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
GCGTGGATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCGCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGG  >  NC_014034/1396456‑1396656

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG