Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,561 C→G 16.1% P148R (CCG→CGG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,5610C→G16.1% 86.2 ‑1.9 ‑0.2224P148R (CCG→CGG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (14/22):  ref base (98/89):  total (113/111)
Fisher's exact test strand distribution p-value = 1.49e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.91e-01

Only 100 of 249 total aligned reads displayed.

GATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1396461‑1396660
                                                                                                     |                                                                                                    
     aaaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgccc.g                                                                                                >  1:520350/1‑101 (MQ=255)
      aaCGGACCTGGAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCAAAACTGCTTTC.GTGGGACGGGCCAGccgccccc                                                                                                >  1:3654518/1‑99 (MQ=255)
      aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgccc.gc                                                                                               >  1:3060254/1‑101 (MQ=255)
      aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgccc.gc                                                                                               <  2:2567817/101‑1 (MQ=255)
      aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGAACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgccc.gc                                                                                               <  1:2950112/101‑1 (MQ=255)
       aCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCc.gcg                                                                                              >  2:475075/1‑101 (MQ=255)
         ggACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGcc                                                                                            >  2:93132/1‑101 (MQ=255)
          gACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCa                                                                                           <  1:950825/101‑1 (MQ=255)
           aCCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCaa                                                                                          <  2:3131673/101‑1 (MQ=255)
             cTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGt                                                                                        >  1:2112474/1‑101 (MQ=255)
             cTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGt                                                                                        >  1:408501/1‑101 (MQ=255)
                aaaTTCCGCGGGAAACGCGGTGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGc                                                                                     >  1:1104376/1‑101 (MQ=255)
                aaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGc                                                                                     >  1:2022688/1‑101 (MQ=255)
                 aaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCt                                                                                    <  1:475132/101‑1 (MQ=255)
                  aTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTc                                                                                   >  1:1764399/1‑101 (MQ=255)
                  aTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTc                                                                                   <  1:3911975/101‑1 (MQ=255)
                   ttCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCAGCGccgacctcggc                                                                                   <  1:789723/101‑10 (MQ=37)
                       gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTcccccc                                                                              <  1:1641167/101‑1 (MQ=255)
                        cgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCCCTccccccc                                                                             >  2:3151363/1‑100 (MQ=255)
                         gggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCcgc                                                                            >  2:3115237/1‑101 (MQ=255)
                          ggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTGCCCCcgcg                                                                           >  2:3206959/1‑101 (MQ=255)
                           gagaCGCGGCGGCGAGCGAGTCGCTCCCCCCGGGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCgg                                                                          <  2:1886236/98‑1 (MQ=38)
                              aCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCCAGTCGCTCCCCCCGCggggg                                                                       >  1:1679214/1‑101 (MQ=255)
                               cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGc                                                                      >  1:758223/1‑101 (MQ=255)
                               cgcgGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCCCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGc                                                                      >  2:1664402/1‑101 (MQ=255)
                                   gcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                                  <  1:2562820/101‑1 (MQ=255)
                                   gcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                                  <  1:593468/101‑1 (MQ=255)
                                   gcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGGgg                                                                  >  2:199098/1‑101 (MQ=255)
                                    ttgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTgg                                                                  >  1:964861/11‑101 (MQ=2)
                                    cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                 >  1:1921959/1‑101 (MQ=255)
                                    cggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                 >  2:3887248/1‑101 (MQ=255)
                                     tgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGa                                                                 <  1:3481420/92‑1 (MQ=2)
                                     gcctcgCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCTGCTTTC.GTGGGACTCGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                <  2:433268/96‑1 (MQ=255)
                                       ggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATc                                                               >  1:710791/8‑101 (MQ=2)
                                        gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                                              >  2:1770830/7‑101 (MQ=2)
                                        gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                             >  2:2307687/1‑101 (MQ=255)
                                          gCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                           <  2:4044722/101‑1 (MQ=255)
                                          gCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                           <  2:1518126/101‑1 (MQ=255)
                                          gCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCCCCCCCCGCGGGGGCGTGGGTCGaaa                                                           >  1:2124528/1‑101 (MQ=255)
                                           ggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGaaa                                                           <  2:12812/98‑1 (MQ=2)
                                           cACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                          >  1:2625086/1‑101 (MQ=255)
                                            gcGTCGCGCCCCCCGCGTGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                         <  1:1887697/100‑1 (MQ=255)
                                             agTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAAct                                                         <  1:3098174/100‑2 (MQ=2)
                                             agTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGGGGATCGAAAct                                                         >  2:1708598/2‑100 (MQ=2)
                                              ggcGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                       <  2:2652742/99‑1 (MQ=255)
                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAt                                                       <  2:4192719/101‑1 (MQ=255)
                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActg                                                        <  1:2323237/101‑3 (MQ=2)
                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                                      <  1:4122007/101‑5 (MQ=2)
                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtg                                                      >  2:566769/1‑97 (MQ=2)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                                    <  2:3966768/101‑1 (MQ=255)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                                    <  1:152770/101‑1 (MQ=255)
                                                  cTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcg                                                   >  2:2198729/1‑101 (MQ=255)
                                                  cTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcg                                                   <  1:2815292/101‑1 (MQ=255)
                                                  cTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgcc                                                    <  1:361102/101‑7 (MQ=2)
                                                   tCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                                  >  2:609393/1‑101 (MQ=255)
                                                   cccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                                  <  2:3257949/100‑1 (MQ=255)
                                                   cccccccGCGGGGGCGGGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                                  <  1:1505668/100‑1 (MQ=255)
                                                    ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                                 >  2:4121428/1‑101 (MQ=255)
                                                    ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                                 >  2:1128107/1‑101 (MQ=255)
                                                    ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                                  <  1:1143593/101‑9 (MQ=2)
                                                    ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                                  >  1:1183267/1‑93 (MQ=2)
                                                     cccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                                 <  1:2389571/101‑10 (MQ=2)
                                                     cccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                                 >  2:3029435/1‑92 (MQ=2)
                                                     cccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                                 >  2:3922592/1‑92 (MQ=2)
                                                      ccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggtt                                                <  1:3733122/101‑11 (MQ=2)
                                                        ccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCtt                                             >  2:1607721/1‑101 (MQ=255)
                                                         cgggggggCGTGGATCGAAACTGCTTTC.GTGCGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                                            <  1:993292/98‑1 (MQ=255)
                                                          gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTACCCCCGCGGGGGCGAGGATAGAAAAAAATTCGTCGCTTac                                           >  1:445185/1‑101 (MQ=37)
                                                             ggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                                        >  1:1037435/1‑101 (MQ=255)
                                                             ggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                                        >  2:3861487/1‑101 (MQ=255)
                                                                gCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGcc                                     <  1:3410336/101‑1 (MQ=255)
                                                                 cGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCt                                    >  2:3434577/1‑101 (MQ=255)
                                                                  gTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                                   >  2:3663005/1‑101 (MQ=255)
                                                                   tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                  >  1:3142320/1‑101 (MQ=255)
                                                                   tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                  >  2:2866427/1‑101 (MQ=255)
                                                                   tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                  >  2:1063163/1‑101 (MQ=255)
                                                                    ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACGATTTAGCCGCTTACGACGCCTATc                                 >  1:3810044/1‑101 (MQ=255)
                                                                     gATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACGATTTCGTCCCCTACGACGCCTCTCt                                >  2:3605997/1‑101 (MQ=38)
                                                                        cGAAACTGCTTTC.GTGGGACTGGCCAGCAGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAt                             >  2:3056537/1‑101 (MQ=255)
                                                                         gAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt                            <  2:2386246/101‑1 (MQ=255)
                                                                         gAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt                            <  1:2164356/101‑1 (MQ=255)
                                                                          aaaCTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                           <  1:1966419/101‑1 (MQ=255)
                                                                          aaaCTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAttt                           >  1:3828199/1‑101 (MQ=255)
                                                                            aCTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCg                         <  2:1418481/101‑1 (MQ=255)
                                                                             cTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCgg                        <  2:2120533/101‑1 (MQ=255)
                                                                               gCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGt                      <  1:871722/101‑1 (MQ=255)
                                                                                cTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTTCGACGCCTATCTGATTTCGGGTc                     >  2:1356501/1‑101 (MQ=255)
                                                                                  ttC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGc                   >  1:3270673/1‑101 (MQ=255)
                                                                                      gTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc                >  2:3785045/1‑101 (MQ=255)
                                                                                      gTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc                >  1:1368723/1‑101 (MQ=255)
                                                                                       tGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTccc               <  2:3530219/101‑1 (MQ=255)
                                                                                           gcTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcg           <  1:2488743/100‑1 (MQ=255)
                                                                                              ggCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCgg        >  2:2478909/1‑101 (MQ=255)
                                                                                               gCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCggg       <  2:2252992/101‑1 (MQ=255)
                                                                                               gCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCggg       <  2:781466/101‑1 (MQ=255)
                                                                                               gCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCggg       >  1:3209246/1‑101 (MQ=255)
                                                                                               gCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGAACGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCggg       >  1:2734862/1‑101 (MQ=255)
                                                                                                ccAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCgggg      <  2:2542618/101‑1 (MQ=255)
                                                                                                  aGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGc    >  2:399668/1‑101 (MQ=255)
                                                                                                   gccgccC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCg   >  1:2803709/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
GATCGAAACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1396461‑1396660

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG