Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,567 C→A 16.2% A150E (GCG→AAC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,5670C→A16.2% 87.0 ‑2.0 ‑0.2216A150E (GCG→GAG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (13/22):  ref base (94/87):  total (107/109)
Fisher's exact test strand distribution p-value = 1.40e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.68e-01

Only 100 of 252 total aligned reads displayed.

AACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1396467‑1396667
                                                                                                      |                                                                                                    
aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCTGCTTTC.GTGGGACTGGCCAGccgccc.gc                                                                                                      >  1:280697/1‑101 (MQ=255)
aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgccc.gc                                                                                                      <  1:2597576/101‑1 (MQ=255)
aaCGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGAACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGccgccc.gc                                                                                                      <  1:2950112/101‑1 (MQ=255)
  cGGCCCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.gcgc                                                                                                    <  2:1745843/101‑1 (MQ=255)
   ggACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGcc                                                                                                   >  2:93132/1‑101 (MQ=255)
    gACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCa                                                                                                  <  1:950825/101‑1 (MQ=255)
     aCCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCaa                                                                                                 >  2:3500584/1‑101 (MQ=255)
       cTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGt                                                                                               >  1:408501/1‑101 (MQ=255)
       cTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGt                                                                                               >  1:2112474/1‑101 (MQ=255)
          aaaTTCCGCGGGAAACGCGGTGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGc                                                                                            >  1:1104376/1‑101 (MQ=255)
          aaaTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGc                                                                                            <  2:4062228/101‑1 (MQ=255)
            aTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTc                                                                                          >  1:1764399/1‑101 (MQ=255)
            aTTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGCCGCTc                                                                                          <  1:270410/101‑1 (MQ=255)
             ttCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCCAGCGccgacctcggc                                                                                          <  1:789723/101‑10 (MQ=37)
             ttCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTcc                                                                                         >  2:363685/1‑101 (MQ=255)
                 gcgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTcccccc                                                                                     <  1:1641167/101‑1 (MQ=255)
                  cgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCcg                                                                                    <  2:2439207/101‑1 (MQ=255)
                  cgGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCCCTccccccc                                                                                    >  2:3151363/1‑100 (MQ=255)
                   gggAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCcgc                                                                                   >  2:3115237/1‑101 (MQ=255)
                       aaCGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCgggg                                                                               <  1:1376638/101‑1 (MQ=255)
                        agctggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCggggg                                                                              >  2:3140138/5‑101 (MQ=255)
                            ggcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTg                                                                          >  2:147744/1‑101 (MQ=255)
                             gcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                                         <  1:2562820/101‑1 (MQ=255)
                             gcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                                         >  2:3904755/1‑101 (MQ=255)
                             gcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                                         <  1:593468/101‑1 (MQ=255)
                             gcggcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTgg                                                                         <  2:2702541/101‑1 (MQ=255)
                              ttgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTgg                                                                         >  1:964861/11‑101 (MQ=2)
                               tgggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGa                                                                        <  1:3481420/92‑1 (MQ=2)
                               gcctcgCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAACCTGCTTTC.GTGGGACTCGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       <  2:433268/96‑1 (MQ=255)
                                gggacggggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGAt                                                                       <  1:3070347/93‑1 (MQ=2)
                                gggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGAt                                                                       <  2:3074343/93‑1 (MQ=2)
                                gcgAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      <  1:3373078/101‑1 (MQ=255)
                                 ggacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATc                                                                      <  2:1531513/94‑1 (MQ=2)
                                  gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                                                     <  2:777493/95‑1 (MQ=2)
                                  gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                                                     <  1:1596964/95‑1 (MQ=2)
                                  gacggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCg                                                                     <  1:63788/95‑1 (MQ=2)
                                  gAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                    >  1:4044983/1‑101 (MQ=255)
                                   gcggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                    <  2:2557425/96‑1 (MQ=2)
                                   acggagTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGa                                                                    <  2:1998846/96‑1 (MQ=2)
                                   aGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                   >  2:1671555/1‑101 (MQ=255)
                                      gcGTCGCGCCCCCCGCGTGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACg                                                                <  1:1887697/100‑1 (MQ=255)
                                       cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGTCT.CCGGGCAGTCGCCTCCCCCGCCGGGGCGCGGTTCGAAAAGa                                                               >  2:3791587/1‑101 (MQ=18)
                                       agTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAAct                                                                <  1:3098174/100‑2 (MQ=2)
                                        gTCGCTCCCACCGCGGGGGCGCGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCCCCC.GCGCCAAGTCGCTCCCCCCCCGTGGGCGTGGATCGACACGac                                                              >  2:477429/1‑100 (MQ=38)
                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAtt                                                             >  1:3511283/1‑101 (MQ=255)
                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGAtt                                                             <  2:3252071/101‑1 (MQ=255)
                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtc                                                           >  1:1740208/1‑101 (MQ=255)
                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgc                                                            >  1:4226149/1‑96 (MQ=2)
                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgc                                                            >  2:164189/1‑96 (MQ=2)
                                             tCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                                         >  2:609393/1‑101 (MQ=255)
                                             cccccccGCGGGGGCGGGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgt                                                         <  1:1505668/100‑1 (MQ=255)
                                              ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                                        >  2:4121428/1‑101 (MQ=255)
                                              ccccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtc                                                        >  2:1128107/1‑101 (MQ=255)
                                              ccccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccgg                                                         >  1:1183267/1‑93 (MQ=2)
                                               cccccGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTtcgtcg                                                       <  1:745205/101‑1 (MQ=255)
                                               cccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                                        >  2:3029435/1‑92 (MQ=2)
                                               cccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                                        >  2:3922592/1‑92 (MQ=2)
                                               cccccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCC.AACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggt                                                        <  1:2389571/101‑10 (MQ=2)
                                                   cgggggggCGTGGATCGAAACTGCTTTC.GTGCGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTa                                                   <  1:993292/98‑1 (MQ=255)
                                                    gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTac                                                  <  2:4207517/101‑1 (MQ=255)
                                                    gcgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTACCCCCGCGGGGGCGAGGATAGAAAAAAATTCGTCGCTTac                                                  >  1:445185/1‑101 (MQ=37)
                                                     cgGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacg                                                 <  2:3991475/101‑1 (MQ=255)
                                                      gggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacga                                                <  2:3592805/101‑1 (MQ=255)
                                                      gggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacga                                                >  2:3410091/1‑101 (MQ=255)
                                                       ggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                                               >  1:1037435/1‑101 (MQ=255)
                                                       ggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                                               <  1:3669610/101‑1 (MQ=255)
                                                       ggggCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTacgac                                               >  2:3861487/1‑101 (MQ=255)
                                                            gTGGATCGAAACTGCTTTC.GTGGGACTGGCCCGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                                          <  1:3207198/101‑1 (MQ=255)
                                                            gTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTa                                          >  2:3663005/1‑101 (MQ=255)
                                                             tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                         >  2:2866427/1‑101 (MQ=255)
                                                             tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                         <  1:1954833/101‑1 (MQ=255)
                                                             tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                         >  2:2488559/1‑101 (MQ=255)
                                                             tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                         <  1:487734/101‑1 (MQ=255)
                                                             tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                         <  1:2250318/101‑1 (MQ=255)
                                                             tGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTAt                                         >  2:1063163/1‑101 (MQ=255)
                                                              ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                                        >  1:11561/1‑101 (MQ=255)
                                                              ggATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATc                                        >  2:2229406/1‑101 (MQ=255)
                                                               gATTGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCt                                       <  1:2123366/101‑1 (MQ=255)
                                                                   gAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGAtt                                   <  1:2164356/101‑1 (MQ=255)
                                                                      aCTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCg                                <  1:1897999/101‑1 (MQ=255)
                                                                         gCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGt                             >  1:2461570/1‑101 (MQ=255)
                                                                         gCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGt                             <  1:871722/101‑1 (MQ=255)
                                                                          cTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTTCGACGCCTATCTGATTTCGGGTc                            >  2:1356501/1‑101 (MQ=255)
                                                                            ttC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGc                          >  2:2605145/1‑101 (MQ=255)
                                                                            ttC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGc                          >  1:3270673/1‑101 (MQ=255)
                                                                             tC.GGGGGACTGGCCGGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCt                         <  2:2197882/101‑1 (MQ=255)
                                                                                gTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcc                       >  2:1912821/1‑101 (MQ=255)
                                                                                 tGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTccc                      <  2:1403651/101‑1 (MQ=255)
                                                                                 tGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTccc                      >  2:1816309/1‑101 (MQ=255)
                                                                                  gggACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTcccc                     >  1:2764017/1‑101 (MQ=255)
                                                                                      cTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgc                 >  2:2293873/1‑101 (MQ=255)
                                                                                       tGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCcgcg                >  1:1982678/1‑101 (MQ=255)
                                                                                          ccAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCgggg             <  1:3824483/101‑1 (MQ=255)
                                                                                          ccAGCCGCCC.GCGCCAAGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGATCCCCCCGCgggg             <  1:3805148/101‑1 (MQ=255)
                                                                                            aGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGc           >  2:399668/1‑101 (MQ=255)
                                                                                            aGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGc           >  2:2340562/1‑101 (MQ=255)
                                                                                              ccgccc.gcGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGt         <  1:3864117/101‑1 (MQ=255)
                                                                                                gccc.gcGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGCCGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTgg       >  1:2862100/1‑101 (MQ=255)
                                                                                                  cc.gcGCCAAGTCGCTCCCCGCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGAt     <  2:520293/101‑1 (MQ=255)
                                                                                                   c.gcgCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCGCCCCCCGCGGGGGCGTGGATc    <  1:3151602/101‑1 (MQ=255)
                                                                                                      |                                                                                                    
AACGGACCTGAAATTCCGCGGGAAACGCGGCGGCGAGCACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCTTTC.GTGGGACTGGCCAGCCGCCC.GCGCCAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGATTTCGTCGCTTACGACGCCTATCTGATTTCGGGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1396467‑1396667

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG