Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,805 T→C 14.0% R229R (CGT→GGC RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,8050T→C14.0% 73.0 ‑0.2 ‑0.2248R229R (CGT→CGCRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (17/18):  ref base (108/105):  total (125/123)
Fisher's exact test strand distribution p-value = 8.57e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.49e-01

Only 100 of 257 total aligned reads displayed.

GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTC  >  NC_014034/1396706‑1396905
                                                                                                   |                                                                                                        
        ccccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGgat...gat                                                                                              <  2:3060029/101‑1 (MQ=255)
         cccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGgatagat                                                                                                >  2:557589/1‑97 (MQ=255)
         cccGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATc                                                                                             <  1:2982180/101‑1 (MQ=255)
           cgcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCAt                                                                                           <  2:1736238/101‑1 (MQ=255)
           cgcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCAt                                                                                           <  2:2763798/101‑1 (MQ=255)
            gcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATg                                                                                          <  1:1117219/101‑1 (MQ=255)
            gcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATg                                                                                          <  2:4103688/101‑1 (MQ=255)
              gggggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCa                                                                                        >  1:952417/1‑101 (MQ=255)
              gggggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCa                                                                                        <  1:2801173/101‑1 (MQ=255)
               ggggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAg                                                                                       <  1:648969/101‑1 (MQ=255)
                 ggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAgcg                                                                                     <  1:363736/101‑1 (MQ=255)
                  gCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAgcgc                                                                                    <  2:2878935/101‑1 (MQ=255)
                    gTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGACTCGCTCCCCCCGCGGGGGCGTGGAGCGAAACCGTGTGGAT...GATCAGGCAGCGCgg                                                                                  <  2:4024003/101‑1 (MQ=255)
                     tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGa                                                                                 >  1:2372301/1‑101 (MQ=255)
                      ggATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGAc                                                                                >  1:2751005/1‑101 (MQ=255)
                       gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACg                                                                               <  1:1333801/101‑1 (MQ=255)
                       gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACg                                                                               <  1:2519387/101‑1 (MQ=255)
                       gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACg                                                                               <  1:3960984/101‑1 (MQ=255)
                        aTCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACgg                                                                              <  2:1751382/101‑1 (MQ=255)
                         tCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGc                                                                             >  1:785885/1‑101 (MQ=255)
                         tCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGc                                                                             <  1:1571517/101‑1 (MQ=255)
                          cGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCt                                                                            >  2:3634436/1‑101 (MQ=255)
                           gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCtt                                                                           <  1:3001409/101‑1 (MQ=255)
                             aaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.gg                                                                        >  1:1821513/1‑101 (MQ=255)
                               cccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGt                                                                      <  1:4003001/101‑1 (MQ=255)
                               cccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGt                                                                      <  2:466183/101‑1 (MQ=255)
                                ccTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTc                                                                     <  1:3691177/101‑1 (MQ=255)
                                 cTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCg                                                                    >  1:4042498/1‑101 (MQ=255)
                                  tGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGCCGGCTT.GGGTCGc                                                                   >  1:1761680/1‑101 (MQ=255)
                                  tGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGc                                                                   >  1:827461/1‑101 (MQ=255)
                                   gTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCt                                                                  <  1:3229958/101‑1 (MQ=255)
                                   gTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCt                                                                  <  2:1357997/101‑1 (MQ=255)
                                        tCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTccccc                                                             >  1:559461/1‑101 (MQ=255)
                                        tCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTccccc                                                             >  2:2547163/1‑101 (MQ=255)
                                          atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCcg                                                           >  1:206690/1‑101 (MQ=255)
                                          atatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCcg                                                           <  1:4221290/101‑1 (MQ=255)
                                           tatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCcgc                                                          >  2:699845/1‑101 (MQ=255)
                                            cttccgatctgATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCcgcg                                                         <  2:3509699/91‑1 (MQ=255)
                                            atGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCcgcg                                                         >  2:3528455/1‑101 (MQ=255)
                                             tgctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCgg                                                        <  2:3223832/101‑1 (MQ=255)
                                              gctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCggg                                                       >  1:3391895/1‑101 (MQ=255)
                                              gctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCggg                                                       >  1:436154/1‑101 (MQ=255)
                                              gctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGCCGCTCCCCCCGCggg                                                       >  2:1495035/1‑101 (MQ=255)
                                               ctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCgggg                                                      <  2:920898/101‑1 (MQ=255)
                                                 gcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCgggggg                                                    >  1:856779/1‑100 (MQ=255)
                                                 gcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGc                                                    <  2:1566451/101‑1 (MQ=255)
                                                   cACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.TGGTCGCTCCCCCCGCGGGGGCgg                                                  >  2:3422229/1‑100 (MQ=255)
                                                   cACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGt                                                  <  2:1634622/101‑1 (MQ=255)
                                                    aCGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTg                                                 <  1:471253/101‑1 (MQ=255)
                                                    aCGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTg                                                 >  2:3934492/1‑101 (MQ=255)
                                                     cGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTgg                                                >  2:1071239/1‑101 (MQ=255)
                                                     cGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGGgg                                                >  1:2260516/1‑101 (MQ=255)
                                                      gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGGGTGGa                                               >  1:1058055/1‑101 (MQ=255)
                                                      gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGa                                               >  1:2621407/1‑101 (MQ=255)
                                                      gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGa                                               >  2:1959849/1‑101 (MQ=255)
                                                      gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGa                                               <  2:22418/101‑1 (MQ=255)
                                                      gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATAATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGGGTGGa                                               >  1:3315933/1‑101 (MQ=255)
                                                          catcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATc                                             >  1:4200071/8‑101 (MQ=2)
                                                           ccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          <  1:1071333/101‑1 (MQ=255)
                                                           atcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                            >  1:1670004/7‑101 (MQ=2)
                                                             ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAc                                        >  1:1293777/1‑101 (MQ=255)
                                                             cgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          <  1:3474012/97‑1 (MQ=2)
                                                             cgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                          <  2:2383602/97‑1 (MQ=2)
                                                              tGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                       <  2:4229183/101‑1 (MQ=255)
                                                              tGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                       >  1:2437361/1‑101 (MQ=255)
                                                              gccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                         >  1:3178015/4‑101 (MQ=2)
                                                              gccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                         >  2:143458/4‑101 (MQ=2)
                                                               ggggcccTCCCCCCGGGGGGGCGTGGATCGAAACCGGGTGGAT...GATCATGCAGCGCGGACGGCTTGGGGCCCCCCCCCCCGCGGGGGCGTGGATCGAAAcc                                       <  2:3752885/95‑1 (MQ=25)
                                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatg                                     >  1:1428498/1‑98 (MQ=2)
                                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGCGGATCCAAAcatg                                     >  2:3700748/1‑98 (MQ=2)
                                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTc                                   <  1:1936127/101‑1 (MQ=255)
                                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTc                                   >  1:2824401/1‑101 (MQ=255)
                                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTc                                   >  1:1847200/1‑101 (MQ=255)
                                                                    gCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggc                                   >  2:1011069/1‑96 (MQ=2)
                                                                     cTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAAcatggct                                  <  1:888322/101‑7 (MQ=2)
                                                                      tCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                               <  1:3576140/101‑1 (MQ=255)
                                                                            cgcgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGa                         <  1:1871525/101‑1 (MQ=255)
                                                                             gcgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAc                        <  2:2153071/101‑1 (MQ=255)
                                                                              cgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAcc                       <  1:3528708/101‑1 (MQ=255)
                                                                               gggggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                      <  2:2937060/101‑1 (MQ=255)
                                                                               gggggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCGGCCCCGGGATAGAccc                      >  2:1525183/1‑100 (MQ=38)
                                                                                ggggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgc                     >  2:1817277/1‑101 (MQ=255)
                                                                                ggggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGGCCAGAGATAGACcgc                     >  2:756717/1‑101 (MQ=255)
                                                                                  ggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgcgc                   <  2:2002214/101‑1 (MQ=255)
                                                                                     gTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGa                >  1:38317/1‑101 (MQ=255)
                                                                                        gATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcct             <  1:529519/101‑1 (MQ=255)
                                                                                        gATCGAAACCGTGTGAAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcct             >  1:709995/1‑101 (MQ=255)
                                                                                         aTCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctc            >  2:3594016/1‑101 (MQ=255)
                                                                                           cGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct          <  2:577833/101‑1 (MQ=255)
                                                                                           cGAAACCGCGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct          >  2:120715/1‑101 (MQ=255)
                                                                                            gAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc         >  2:1865115/1‑101 (MQ=255)
                                                                                            gAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc         >  2:1423221/1‑101 (MQ=255)
                                                                                             aaaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa        >  2:3280728/1‑101 (MQ=255)
                                                                                             aaaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa        <  2:2694565/101‑1 (MQ=255)
                                                                                             aaaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa        >  2:4140656/1‑101 (MQ=255)
                                                                                              aaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg       <  1:1901648/101‑1 (MQ=255)
                                                                                              aaCCGTGTGGAT...GATCATGCAGCGCGGACGGCGT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg       <  2:2097041/101‑1 (MQ=255)
                                                                                               aCCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTAGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGa      <  2:383395/101‑1 (MQ=255)
                                                                                               aCCGTGTGGAG...TATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGa      <  2:3353810/101‑1 (MQ=255)
                                                                                                  ctgtgGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGt   <  2:3985650/100‑1 (MQ=255)
                                                                                                   |                                                                                                        
GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTC  >  NC_014034/1396706‑1396905

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG