Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,816 A→T 13.4% H233L (CAT→CTG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,8160A→T13.4% 98.4 ‑0.3 ‑0.2253H233L (CAT→CTT) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (16/18):  ref base (113/106):  total (129/124)
Fisher's exact test strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.81e-01

Only 100 of 259 total aligned reads displayed.

CCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCC  >  NC_014034/1396716‑1396914
                                                                                                       |                                                                                                   
ccGCGGGGGCGTGGATCGAATCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCa                                                                                                     <  2:1968310/101‑1 (MQ=255)
 cgcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCAt                                                                                                    <  2:1736238/101‑1 (MQ=255)
  gcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATTGAAACCGTGTGGAT...GATCATg                                                                                                   <  2:2901205/101‑1 (MQ=255)
  gcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATg                                                                                                   <  1:1117219/101‑1 (MQ=255)
   cgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCAtag                                                                                                  >  2:845036/1‑99 (MQ=255)
   cgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGc                                                                                                  >  2:1483391/1‑101 (MQ=255)
    gggggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCa                                                                                                 <  1:3830830/101‑1 (MQ=255)
     ggggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAg                                                                                                <  1:648969/101‑1 (MQ=255)
      gggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAgc                                                                                               <  2:4127155/101‑1 (MQ=255)
      gggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAgc                                                                                               <  1:2879316/101‑1 (MQ=255)
          gTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGACTCGCTCCCCCCGCGGGGGCGTGGAGCGAAACCGTGTGGAT...GATCAGGCAGCGCgg                                                                                           <  2:4024003/101‑1 (MQ=255)
           tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGa                                                                                          >  1:3467136/1‑101 (MQ=255)
           tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGa                                                                                          >  1:408097/1‑101 (MQ=255)
           tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGa                                                                                          >  2:295056/1‑101 (MQ=255)
            ggATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCggcc                                                                                         >  2:755081/1‑99 (MQ=255)
             gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACg                                                                                        <  1:1333801/101‑1 (MQ=255)
             gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACg                                                                                        >  1:96274/1‑101 (MQ=255)
             gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACg                                                                                        >  1:2427950/1‑101 (MQ=255)
              aTCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACgg                                                                                       <  2:1751382/101‑1 (MQ=255)
               tCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGc                                                                                      <  1:81719/101‑1 (MQ=255)
               tCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGc                                                                                      <  1:1571517/101‑1 (MQ=255)
                 gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCtt                                                                                    >  1:3985910/1‑101 (MQ=255)
                 gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCtt                                                                                    <  1:3001409/101‑1 (MQ=255)
                   aaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.gg                                                                                 <  1:1741206/101‑1 (MQ=255)
                   aaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.gg                                                                                 >  1:1821513/1‑101 (MQ=255)
                    aCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.ggg                                                                                >  2:3730648/1‑101 (MQ=255)
                      cctgtatcccATAGGCTGCCACGATGCCCTCGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTc                                                                              <  2:2051277/92‑1 (MQ=37)
                       cTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCg                                                                             <  1:890686/101‑1 (MQ=255)
                        tGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGCCGGCTT.GGGTCGc                                                                            >  1:1761680/1‑101 (MQ=255)
                        tGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGc                                                                            >  1:827461/1‑101 (MQ=255)
                        tGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGc                                                                            <  2:3654265/101‑1 (MQ=255)
                         gTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCt                                                                           <  1:3229958/101‑1 (MQ=255)
                          tCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTc                                                                          >  1:2002340/1‑101 (MQ=255)
                           cccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTcc                                                                         <  1:2332316/101‑1 (MQ=255)
                             cTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTcccc                                                                       <  2:1515025/101‑1 (MQ=255)
                              tCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTccccc                                                                      >  2:2547163/1‑101 (MQ=255)
                                 tatGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCcgc                                                                   >  2:2248677/1‑101 (MQ=255)
                                  cttccgatctgATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCcgcg                                                                  <  2:3509699/91‑1 (MQ=255)
                                  atGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCcgcg                                                                  >  2:2725756/1‑101 (MQ=255)
                                    gctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCggg                                                                >  1:2734794/1‑101 (MQ=255)
                                     ctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCgggg                                                               <  2:920898/101‑1 (MQ=255)
                                         cACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGt                                                           <  2:1634622/101‑1 (MQ=255)
                                          aCGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTg                                                          <  1:471253/101‑1 (MQ=255)
                                          aCGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTg                                                          >  2:3934492/1‑101 (MQ=255)
                                          aCGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTg                                                          <  1:3678953/101‑1 (MQ=255)
                                           cGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTgg                                                         >  2:1071239/1‑101 (MQ=255)
                                            gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCcgcgagatcggaag                                                        >  1:3509952/1‑91 (MQ=255)
                                            gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGGGTGGa                                                        >  1:1058055/1‑101 (MQ=255)
                                              cacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGa                                                        >  1:3339473/10‑101 (MQ=0)
                                               gCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGCTCCAAACCGCCTGGGT...GATTATGCACCCCGGGCGGCGT.GGTCGGCTGCCCCCGCcggggggcgggagg                                                     >  2:3295838/1‑92 (MQ=12)
                                                 ccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                   <  1:1071333/101‑1 (MQ=255)
                                                 ccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                   <  1:1401152/101‑1 (MQ=255)
                                                   ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATGGaacc                                                 >  2:4200913/1‑99 (MQ=255)
                                                   ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAc                                                 >  1:1293777/1‑101 (MQ=255)
                                                    gccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  >  1:3178015/4‑101 (MQ=2)
                                                     ggggcccTCCCCCCGGGGGGGCGTGGATCGAAACCGGGTGGAT...GATCATGCAGCGCGGACGGCTTGGGGCCCCCCCCCCCGCGGGGGCGTGGATCGAAAcc                                                <  2:3752885/95‑1 (MQ=25)
                                                     gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                               <  2:1952626/101‑1 (MQ=255)
                                                     ccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                 <  2:651385/99‑1 (MQ=2)
                                                       gccGCTCCCCCCGCGGGGGCGTGGAGCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                               <  1:2107128/99‑3 (MQ=2)
                                                       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                               <  1:2467971/101‑3 (MQ=2)
                                                       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                               <  2:2561632/101‑3 (MQ=2)
                                                       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGt                                             >  1:2772771/1‑101 (MQ=255)
                                                        tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatg                                              <  2:3549510/101‑4 (MQ=2)
                                                        tCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTc                                            >  1:1847200/1‑101 (MQ=255)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                          >  1:746254/1‑101 (MQ=255)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggct                                           <  1:2607577/101‑7 (MQ=2)
                                                            tCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                                        >  2:425219/1‑101 (MQ=255)
                                                             ccccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctat                                         >  1:566797/1‑93 (MQ=2)
                                                              cccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAgaga                                      <  1:3934752/101‑1 (MQ=255)
                                                               ccccGCGGGGGGGGGGATCGAAACCGTGTGGAT...GGTCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGAt                                     <  1:1912947/101‑1 (MQ=255)
                                                                 ccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAg                                   <  2:3997319/101‑1 (MQ=255)
                                                                  cgcgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGa                                  <  1:1871525/101‑1 (MQ=255)
                                                                    cgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAcc                                <  1:3528708/101‑1 (MQ=255)
                                                                     gggggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                               >  1:3282384/1‑101 (MQ=255)
                                                                      ggggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgc                              >  2:1817277/1‑101 (MQ=255)
                                                                      ggggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgc                              <  2:1549369/101‑1 (MQ=255)
                                                                          cGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCgg                          >  2:56064/1‑101 (MQ=255)
                                                                          cGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCgg                          >  1:4021350/1‑101 (MQ=255)
                                                                           gTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGa                         >  1:8620/1‑101 (MQ=255)
                                                                            tGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCgggc                        >  2:3883074/1‑99 (MQ=255)
                                                                            tGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                        <  1:4212544/101‑1 (MQ=255)
                                                                             ggATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcc                       >  1:281535/1‑101 (MQ=255)
                                                                              gATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcct                      <  2:3241711/101‑1 (MQ=255)
                                                                              gATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcct                      <  1:529519/101‑1 (MQ=255)
                                                                              gATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcct                      >  1:3593859/1‑101 (MQ=255)
                                                                               aTCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctc                     >  2:3594016/1‑101 (MQ=255)
                                                                               aTCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctc                     >  2:1386094/1‑101 (MQ=255)
                                                                                 cGAAACCGCGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct                   >  2:120715/1‑101 (MQ=255)
                                                                                  gAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                  >  2:1865115/1‑101 (MQ=255)
                                                                                  gAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                  >  2:1423221/1‑101 (MQ=255)
                                                                                   aaaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                 >  2:3431729/1‑101 (MQ=255)
                                                                                   aaaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                 <  2:2180702/101‑1 (MQ=255)
                                                                                   aaaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                 <  1:425276/101‑1 (MQ=255)
                                                                                    aaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg                <  2:408046/101‑1 (MQ=255)
                                                                                    aaCCGTGTGGAT...GATCATGCAGCGCGGACGGCGT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg                <  2:2097041/101‑1 (MQ=255)
                                                                                     aCCGTGTGGAG...TATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGa               <  2:3353810/101‑1 (MQ=255)
                                                                                          gtgGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCg          >  2:2400244/1‑101 (MQ=255)
                                                                                               t...gatCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccc     <  2:2772552/101‑1 (MQ=255)
                                                                                                    atCATGCAGCGCGGACGGCTT.GGGACGCTCCCCCGGCGGGGGCGTGGATCAAAAACGGTCCAGAGATAGACCGCGCGGACCTCCGCAGACGTCGCTccccc   >  2:2824867/1‑101 (MQ=255)
                                                                                                     tCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcccccc  >  1:3892199/1‑101 (MQ=255)
                                                                                                       |                                                                                                   
CCGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCC  >  NC_014034/1396716‑1396914

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG