Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,817 T→G 14.0% H233Q (CAT→CTG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,8170T→G14.0% 94.0 ‑0.7 ‑0.1249H233Q (CAT→CAG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (16/19):  ref base (112/102):  total (128/121)
Fisher's exact test strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.80e-01

Only 100 of 259 total aligned reads displayed.

CGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGC  >  NC_014034/1396717‑1396916
                                                                                                       |                                                                                                    
cgcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCAt                                                                                                      <  2:1736238/101‑1 (MQ=255)
 gcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATTGAAACCGTGTGGAT...GATCATg                                                                                                     <  2:2901205/101‑1 (MQ=255)
 gcgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATg                                                                                                     <  1:1117219/101‑1 (MQ=255)
  cgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCAtag                                                                                                    >  2:845036/1‑99 (MQ=255)
  cgGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGc                                                                                                    >  2:1483391/1‑101 (MQ=255)
   gggggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCa                                                                                                   <  1:3830830/101‑1 (MQ=255)
    ggggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAg                                                                                                  <  1:648969/101‑1 (MQ=255)
     gggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAgc                                                                                                 <  2:4127155/101‑1 (MQ=255)
     gggCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAgc                                                                                                 <  1:2879316/101‑1 (MQ=255)
         gTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGACTCGCTCCCCCCGCGGGGGCGTGGAGCGAAACCGTGTGGAT...GATCAGGCAGCGCgg                                                                                             <  2:4024003/101‑1 (MQ=255)
          tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGa                                                                                            >  2:295056/1‑101 (MQ=255)
          tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGa                                                                                            >  1:3467136/1‑101 (MQ=255)
          tGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGa                                                                                            >  1:408097/1‑101 (MQ=255)
           ggATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCggcc                                                                                           >  2:755081/1‑99 (MQ=255)
            gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACg                                                                                          >  1:96274/1‑101 (MQ=255)
            gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACg                                                                                          <  1:1333801/101‑1 (MQ=255)
            gATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACg                                                                                          >  1:2427950/1‑101 (MQ=255)
             aTCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACgg                                                                                         <  2:1751382/101‑1 (MQ=255)
              tCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGc                                                                                        <  1:81719/101‑1 (MQ=255)
              tCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGc                                                                                        <  1:1571517/101‑1 (MQ=255)
                gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCtt                                                                                      <  1:3001409/101‑1 (MQ=255)
                gAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCtt                                                                                      >  1:3985910/1‑101 (MQ=255)
                 aaaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.g                                                                                    <  2:2226143/101‑1 (MQ=255)
                  aaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.gg                                                                                   >  1:1821513/1‑101 (MQ=255)
                  aaCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.gg                                                                                   <  1:1741206/101‑1 (MQ=255)
                   aCCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.ggg                                                                                  >  2:3730648/1‑101 (MQ=255)
                      cTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCg                                                                               <  1:890686/101‑1 (MQ=255)
                       tGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGCCGGCTT.GGGTCGc                                                                              >  1:1761680/1‑101 (MQ=255)
                       tGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGc                                                                              <  2:3654265/101‑1 (MQ=255)
                       tGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGc                                                                              >  1:827461/1‑101 (MQ=255)
                        gTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCt                                                                             <  1:3229958/101‑1 (MQ=255)
                         tCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTc                                                                            >  1:2002340/1‑101 (MQ=255)
                          cccTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTcc                                                                           <  1:2332316/101‑1 (MQ=255)
                            cTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTcccc                                                                         <  2:1515025/101‑1 (MQ=255)
                                 cttccgatctgATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCcgcg                                                                    <  2:3509699/91‑1 (MQ=255)
                                   gctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCggg                                                                  >  1:2734794/1‑101 (MQ=255)
                                    ctgcCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCgggg                                                                 <  2:920898/101‑1 (MQ=255)
                                        cACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGt                                                             <  2:1634622/101‑1 (MQ=255)
                                         aCGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTg                                                            <  1:471253/101‑1 (MQ=255)
                                         aCGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTg                                                            >  2:3934492/1‑101 (MQ=255)
                                         aCGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTg                                                            <  1:3678953/101‑1 (MQ=255)
                                          cGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTgg                                                           <  2:2124383/101‑1 (MQ=255)
                                          cGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTgg                                                           >  2:1071239/1‑101 (MQ=255)
                                           gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCcgcgagatcggaag                                                          >  1:3509952/1‑91 (MQ=255)
                                           gATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGGGTGGa                                                          >  1:1058055/1‑101 (MQ=255)
                                             cacatcgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGa                                                          >  1:3339473/10‑101 (MQ=0)
                                              gCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGCTCCAAACCGCCTGGGT...GATTATGCACCCCGGGCGGCGT.GGTCGGCTGCCCCCGCcggggggcgggagg                                                       >  2:3295838/1‑92 (MQ=12)
                                                ccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     <  1:1071333/101‑1 (MQ=255)
                                                ccTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     <  1:1401152/101‑1 (MQ=255)
                                                  ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATGGaacc                                                   >  2:4200913/1‑99 (MQ=255)
                                                  ttGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGGGGATCGAAAc                                                   >  1:1293777/1‑101 (MQ=255)
                                                  cgccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     <  2:2383602/97‑1 (MQ=2)
                                                   gccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    >  1:3178015/4‑101 (MQ=2)
                                                    ggggcccTCCCCCCGGGGGGGCGTGGATCGAAACCGGGTGGAT...GATCATGCAGCGCGGACGGCTTGGGGCCCCCCCCCCCGCGGGGGCGTGGATCGAAAcc                                                  <  2:3752885/95‑1 (MQ=25)
                                                    gAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCg                                                 <  2:1952626/101‑1 (MQ=255)
                                                    ccgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                   <  2:651385/99‑1 (MQ=2)
                                                      gccGCTCCCCCCGCGGGGGCGTGGAGCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                                 <  1:2107128/99‑3 (MQ=2)
                                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcat                                                 <  1:2467971/101‑3 (MQ=2)
                                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGt                                               >  1:2772771/1‑101 (MQ=255)
                                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatg                                                <  2:3549510/101‑4 (MQ=2)
                                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatg                                                >  2:2553466/1‑98 (MQ=2)
                                                       tCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTc                                              >  1:1847200/1‑101 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCa                                            >  1:746254/1‑101 (MQ=255)
                                                          cTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggct                                             <  1:2607577/101‑7 (MQ=2)
                                                           tCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCaga                                          >  2:425219/1‑101 (MQ=255)
                                                            ccccccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGG.TT.GCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcatggctat                                           >  1:566797/1‑93 (MQ=2)
                                                             cccccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAgaga                                        <  1:3934752/101‑1 (MQ=255)
                                                              ccccGCGGGGGGGGGGATCGAAACCGTGTGGAT...GGTCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGAt                                       <  1:1912947/101‑1 (MQ=255)
                                                                ccGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAg                                     <  2:3997319/101‑1 (MQ=255)
                                                                 cgcgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGa                                    <  1:1871525/101‑1 (MQ=255)
                                                                   cgGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGAcc                                  <  1:3528708/101‑1 (MQ=255)
                                                                    gggggCGTGGCTCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                                 <  2:2092230/101‑1 (MQ=255)
                                                                    gggggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcg                                 >  1:3282384/1‑101 (MQ=255)
                                                                     ggggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgc                                >  2:1817277/1‑101 (MQ=255)
                                                                     ggggCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACcgc                                <  2:1549369/101‑1 (MQ=255)
                                                                         cGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCgg                            >  2:56064/1‑101 (MQ=255)
                                                                         cGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCgg                            >  1:4021350/1‑101 (MQ=255)
                                                                          gTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGa                           >  1:8620/1‑101 (MQ=255)
                                                                           tGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCgggc                          >  2:3883074/1‑99 (MQ=255)
                                                                           tGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAc                          <  1:4212544/101‑1 (MQ=255)
                                                                            ggATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcc                         >  1:281535/1‑101 (MQ=255)
                                                                             gATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcct                        <  2:3241711/101‑1 (MQ=255)
                                                                             gATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcct                        <  1:529519/101‑1 (MQ=255)
                                                                             gATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcct                        >  1:3593859/1‑101 (MQ=255)
                                                                              aTCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctc                       >  2:3594016/1‑101 (MQ=255)
                                                                              aTCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctc                       >  2:1386094/1‑101 (MQ=255)
                                                                                cGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct                     >  2:245945/1‑101 (MQ=255)
                                                                                cGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct                     >  2:2791497/1‑101 (MQ=255)
                                                                                cGAAACCGCGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGAcctcct                     >  2:120715/1‑101 (MQ=255)
                                                                                 gAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                    >  2:1865115/1‑101 (MQ=255)
                                                                                 gAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACctcctc                    >  2:1423221/1‑101 (MQ=255)
                                                                                  aaaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                   <  1:425276/101‑1 (MQ=255)
                                                                                  aaaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCa                   >  2:3431729/1‑101 (MQ=255)
                                                                                   aaCCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAg                  <  2:408046/101‑1 (MQ=255)
                                                                                    aCCGTGTGGAG...TATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGa                 <  2:3353810/101‑1 (MQ=255)
                                                                                         gtgGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCg            >  2:1993609/1‑101 (MQ=255)
                                                                                                   atCATGCAGCGCGGGCGGCTT.GGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccccc     <  2:2734656/101‑1 (MQ=255)
                                                                                                   atCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTccccc     <  2:2621215/101‑1 (MQ=255)
                                                                                                   atCATGCAGCGCGGACGGCTT.GGGACGCTCCCCCGGCGGGGGCGTGGATCAAAAACGGTCCAGAGATAGACCGCGCGGACCTCCGCAGACGTCGCTccccc     >  2:2824867/1‑101 (MQ=255)
                                                                                                    tCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTcccccc    >  1:3892199/1‑101 (MQ=255)
                                                                                                       |                                                                                                    
CGCGGGGGCGTGGATCGAAACCCTGTCCCTCATATGCTGCCACGATGCCCTTGAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGTGTGGAT...GATCATGCAGCGCGGACGGCTT.GGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGC  >  NC_014034/1396717‑1396916

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG