Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,937 C→A 15.9% S273R (AGC→AGA RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9370C→A15.9% 104.9 ‑0.1 ‑0.1288S273R (AGC→AGARCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/22):  ref base (104/112):  total (139/149)
Fisher's exact test strand distribution p-value = 8.66e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.18e-01

Only 100 of 314 total aligned reads displayed.

TCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCG  >  NC_014034/1396837‑1397037
                                                                                                     |                                                                                                              
  gccccccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCt                                                                                                              <  1:2818221/98‑1 (MQ=255)
   cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.g                                                                                                            <  2:1071502/101‑1 (MQ=255)
    tCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gc                                                                                                           >  2:1145627/1‑101 (MQ=255)
    gccccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gc                                                                                                           <  2:3615435/100‑1 (MQ=255)
     ccccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gcg                                                                                                          <  1:304367/101‑1 (MQ=255)
          cgcgGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.Gccgtcttgagt                                                                                                      <  1:3357597/101‑10 (MQ=25)
              ggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.t                                                                                              <  1:1864599/101‑1 (MQ=255)
               gggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.Tc                                                                                             <  1:3894115/101‑1 (MQ=255)
                  cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGTTCCTCCTCAGCCGTCGCTCCCCCCACGGAGGCGTCGACAGAAACA.GCCT.GCGCG.GC.CGCA.TCGGc                                                                                          >  2:1391225/1‑101 (MQ=255)
                   gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCt                                                                                         >  1:3098189/1‑101 (MQ=255)
                    tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCt.t                                                                                       >  2:1947318/1‑101 (MQ=255)
                    tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCt.t                                                                                       >  2:639601/1‑101 (MQ=255)
                         cGAAACCGGTCCAGAGATAGACCGCGCGGACGTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGc.c                                                                                >  1:3749821/1‑101 (MQ=255)
                           aaaCCGGTCCAGAGATAGACCGCGCGTACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTg                                                                              <  1:2547354/101‑1 (MQ=255)
                            aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..c                                                                           <  2:2637928/101‑1 (MQ=255)
                            aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..c                                                                           <  2:2734588/101‑1 (MQ=255)
                                  tCCAGAGATAGACAGCGCGGACCTCCTCAGACGAGGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTc                                                                    <  1:3634689/101‑1 (MQ=255)
                                  gccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTc                                                                    <  1:981186/100‑1 (MQ=255)
                                    cAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GGCGc                                                                  >  2:4038710/1‑101 (MQ=255)
                                     agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCt                                                                 >  2:3899840/1‑101 (MQ=255)
                                      gagaTAGACCGCTCGGACCTCCTCAGACGCCGCTCCCCCTGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGG.CTG..CGTG.GTCGCTc                                                                >  2:4178209/1‑101 (MQ=38)
                                         aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTcccc                                                             <  2:3502560/101‑1 (MQ=255)
                                         aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GCCGCTcccc                                                             >  2:3472049/1‑101 (MQ=255)
                                          tAGCCCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTccccc                                                            <  2:824837/101‑1 (MQ=255)
                                          tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTccccc                                                            >  1:2162195/1‑101 (MQ=255)
                                          tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTccccc                                                            >  2:4131773/1‑101 (MQ=255)
                                             aCCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCcgc                                                         <  1:4038971/101‑1 (MQ=255)
                                               cgcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCgg                                                       <  2:701552/101‑1 (MQ=255)
                                               cgcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGCG.GTCGCTCCCCCCGCgg                                                       >  2:2032607/1‑101 (MQ=255)
                                                gcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCggg                                                      >  2:2183098/1‑101 (MQ=255)
                                                  gcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCggggg                                                    >  2:1222752/1‑101 (MQ=255)
                                                   cgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                   >  2:1772284/1‑101 (MQ=255)
                                                   cgGACCTCCTCAGACGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                   <  2:1882480/101‑1 (MQ=255)
                                                    ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                  >  2:950168/1‑101 (MQ=255)
                                                       cctcctCAGACGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                               <  1:1145721/101‑1 (MQ=255)
                                                        tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                               <  1:58498/92‑1 (MQ=0)
                                                        ctcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                              <  1:2127599/101‑1 (MQ=255)
                                                        attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                              <  1:290828/91‑1 (MQ=0)
                                                        attggccccggTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                              <  1:1786308/91‑1 (MQ=0)
                                                         ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                             <  1:777828/92‑1 (MQ=2)
                                                         tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                             >  1:1018959/1‑101 (MQ=255)
                                                          tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  1:875586/93‑1 (MQ=2)
                                                          gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                             <  2:1243074/94‑1 (MQ=2)
                                                          ccccgcccGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTgg                                               <  1:268214/94‑1 (MQ=0)
                                                           ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  2:3232107/94‑1 (MQ=2)
                                                           ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  2:1612696/94‑1 (MQ=2)
                                                           cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGaag                                            >  2:184614/7‑99 (MQ=2)
                                                           cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  1:1343515/95‑1 (MQ=2)
                                                           caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  2:2465886/94‑1 (MQ=9)
                                                            aacactgTCGCTCCCCCCGCGGGGGGGTGGATCGAAAC..GCGG.GCCCGCGC.CGCG.CCGGATCTT.TGT.CGG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAttg                                           >  1:4135029/7‑99 (MQ=2)
                                                            aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                           <  2:3375370/95‑1 (MQ=9)
                                                             gttgcGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                          <  1:4090654/97‑1 (MQ=2)
                                                             cgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                            <  2:764498/97‑1 (MQ=2)
                                                             cagccgtgccTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         <  2:3282143/92‑1 (MQ=255)
                                                             cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         <  2:3867013/101‑1 (MQ=255)
                                                             cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                         >  2:1314058/1‑101 (MQ=255)
                                                              aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                        >  2:1681531/1‑101 (MQ=255)
                                                                gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                       <  2:2561397/100‑1 (MQ=2)
                                                                 tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                      >  1:1676223/2‑100 (MQ=9)
                                                                 tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                      <  2:3726950/100‑2 (MQ=9)
                                                                 tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                      <  2:2848824/100‑2 (MQ=9)
                                                                 tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                      <  2:2383205/100‑2 (MQ=9)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccg                                    >  2:437598/1‑98 (MQ=2)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActaca                                   <  1:2235987/101‑5 (MQ=2)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActaca                                   <  1:7030/101‑5 (MQ=2)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActaca                                   >  1:500958/1‑97 (MQ=2)
                                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcg                                    <  1:3387187/101‑4 (MQ=9)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                     <  2:96623/101‑2 (MQ=2)
                                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                   >  1:1551124/1‑97 (MQ=9)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaaaccc                                    >  2:3421640/1‑97 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.CGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCCGGGGCGTGGATccgaacccccc                                  >  2:1566110/1‑91 (MQ=2)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACATCGGc                                 >  2:3566385/1‑101 (MQ=255)
                                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                 >  1:3937694/1‑95 (MQ=2)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GGGCG.GCATGGATTCATCA.AC.CGC.CAT..CGAGCGTCCCTCCCCCCGCGGGGGCGTGGGACGaaaaacca                                   >  2:2610838/1‑96 (MQ=2)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                 <  2:3087523/101‑7 (MQ=2)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                 >  1:466959/1‑95 (MQ=2)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                 <  2:3338866/101‑7 (MQ=2)
                                                                      cTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcc                                 >  1:1390635/1‑95 (MQ=9)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                               >  1:287457/1‑101 (MQ=255)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                               >  1:1083388/1‑93 (MQ=2)
                                                                       tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                >  2:505930/1‑94 (MQ=9)
                                                                       cccccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                               <  2:1102975/100‑9 (MQ=2)
                                                                        ccccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                 <  1:1693580/101‑3 (MQ=2)
                                                                        ccccccGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacg                               >  1:658081/1‑93 (MQ=0)
                                                                        ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                               <  1:1972157/101‑9 (MQ=9)
                                                                         cccccGCGGGGGCGTGGATCGAAACA.CCCG.GCGCC.GC.AGCG.AGTGCG.TCTTGA.ACA..GGAT.GTCGCTCCCCCCGCAGGGGCGTGGATCGAAActatgctggc                              >  1:3551874/1‑92 (MQ=2)
                                                                           cccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgcc                              <  1:422083/101‑6 (MQ=0)
                                                                             cgcgGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                            <  1:1934933/101‑8 (MQ=0)
                                                                             cgcgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACATCAGCTCGTCGtt                         <  1:2400420/101‑1 (MQ=255)
                                                                             cgcgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                         <  2:2427774/101‑1 (MQ=255)
                                                                               cgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                       >  1:3257945/1‑101 (MQ=255)
                                                                               cgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                       <  2:2891677/101‑1 (MQ=255)
                                                                                gggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                      >  1:391132/1‑101 (MQ=255)
                                                                                  gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                    <  2:3165396/101‑1 (MQ=255)
                                                                                    gCGTGGATCGAACCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTGGCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                  <  1:4178471/101‑1 (MQ=255)
                                                                                    gCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                  <  2:2961625/101‑1 (MQ=255)
                                                                                      gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                >  1:1051132/1‑101 (MQ=255)
                                                                                                aCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc      <  1:3699062/101‑1 (MQ=255)
                                                                                                    gCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc   >  1:1747365/1‑101 (MQ=255)
                                                                                                    gCCG.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc   <  1:1275808/101‑1 (MQ=255)
                                                                                                     |                                                                                                              
TCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCG  >  NC_014034/1396837‑1397037

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG