Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,940 G→T 10.7% L274L (CTG→TGT RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9400G→T10.7% 69.8 0.0 ‑1.1277L274L (CTG→CTTRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (15/15):  ref base (121/126):  total (136/141)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.83e-02

Only 100 of 311 total aligned reads displayed.

CTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTC  >  NC_014034/1396840‑1397040
                                                                                                      |                                                                                                            
cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.g                                                                                                              <  2:1071502/101‑1 (MQ=255)
cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.g                                                                                                              <  2:3310943/101‑1 (MQ=255)
cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.g                                                                                                              >  2:3450607/1‑101 (MQ=255)
   cccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gcgc                                                                                                           <  1:3959271/101‑1 (MQ=255)
      ccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAGA.GCCT.GCGCG.Gc                                                                                                       >  1:3274240/1‑101 (MQ=255)
       cgcgGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.Gc.c                                                                                                     <  1:699922/101‑1 (MQ=255)
           ggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.t                                                                                                <  1:1864599/101‑1 (MQ=255)
            gggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.Tc                                                                                               <  1:3894115/101‑1 (MQ=255)
            gggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.Tc                                                                                               >  2:363464/1‑101 (MQ=255)
                gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCt                                                                                           >  2:3125390/1‑101 (MQ=255)
                 tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCt.t                                                                                         >  2:1947318/1‑101 (MQ=255)
                 tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCt.t                                                                                         >  1:3165635/1‑101 (MQ=255)
                      cGAAACCGGTCCAGAGATAGACCGCGCGGACGTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGc.c                                                                                   >  1:3749821/1‑101 (MQ=255)
                      cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGc.c                                                                                   <  2:2861881/101‑1 (MQ=255)
                       gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                  <  2:746177/101‑1 (MQ=255)
                         aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..c                                                                              <  2:2637928/101‑1 (MQ=255)
                         aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..c                                                                              <  2:2734588/101‑1 (MQ=255)
                            cGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTg                                                                           >  2:1126893/1‑101 (MQ=255)
                             ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CCG..CGTg.g                                                                         >  1:2891896/1‑101 (MQ=255)
                               gccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTc                                                                       <  1:981186/100‑1 (MQ=255)
                                 cAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGc                                                                     >  2:417198/1‑101 (MQ=255)
                                 cAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GGCGc                                                                     >  2:4038710/1‑101 (MQ=255)
                                  agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCt                                                                    >  2:3899840/1‑101 (MQ=255)
                                     gaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccc                                                                 <  1:2725962/101‑1 (MQ=255)
                                      aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GGCGCTcccc                                                                >  2:3204558/1‑101 (MQ=255)
                                        aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                              <  1:169125/101‑1 (MQ=255)
                                        aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                              <  1:2825086/101‑1 (MQ=255)
                                            cgcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGCG.GTCGCTCCCCCCGCgg                                                          >  2:2032607/1‑101 (MQ=255)
                                                cgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                      >  2:1772284/1‑101 (MQ=255)
                                                cgGACCTCCTCAGACGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                      <  2:1882480/101‑1 (MQ=255)
                                                 ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                     >  2:950168/1‑101 (MQ=255)
                                                  gccctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGt                                                    <  1:1601367/99‑1 (MQ=255)
                                                   aCCTCCTCCGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTg                                                   <  2:432769/101‑1 (MQ=255)
                                                    cctcctCAGACGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                  <  1:1145721/101‑1 (MQ=255)
                                                     tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                  <  2:2948618/92‑1 (MQ=0)
                                                     attggccccggTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                 <  1:1786308/91‑1 (MQ=0)
                                                      ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                <  1:777828/92‑1 (MQ=2)
                                                      tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                >  2:1578004/1‑101 (MQ=255)
                                                      tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                >  1:1018959/1‑101 (MQ=255)
                                                       tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                               <  1:875586/93‑1 (MQ=2)
                                                       gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                <  2:1243074/94‑1 (MQ=2)
                                                        cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGaag                                               >  2:184614/7‑99 (MQ=2)
                                                        cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                               <  1:1343515/95‑1 (MQ=2)
                                                        caactctgTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                               <  1:2149614/94‑1 (MQ=9)
                                                        caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                               <  2:2465886/94‑1 (MQ=9)
                                                         aacactgTCGCTCCCCCCGCGGGGGGGTGGATCGAAAC..GCGG.GCCCGCGC.CGCG.CCGGATCTTTGT.CGG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAttg                                              >  1:4135029/7‑99 (MQ=2)
                                                         aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                              <  2:3375370/95‑1 (MQ=9)
                                                          gttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                             <  1:2234409/97‑1 (MQ=2)
                                                          gttgcGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                             <  1:4090654/97‑1 (MQ=2)
                                                          ccccggTCGCTCACCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                            >  1:2289869/6‑101 (MQ=2)
                                                          cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                            <  2:3867013/101‑1 (MQ=255)
                                                          cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                            >  2:1314058/1‑101 (MQ=255)
                                                          cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                            >  1:3035506/1‑101 (MQ=255)
                                                          acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                             >  2:3598272/6‑101 (MQ=9)
                                                           aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                           >  2:1681531/1‑101 (MQ=255)
                                                             gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                          <  2:2561397/100‑1 (MQ=2)
                                                             cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                         <  2:594928/99‑2 (MQ=2)
                                                              tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                         <  2:2383205/100‑2 (MQ=9)
                                                              tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                         <  2:3726950/100‑2 (MQ=9)
                                                              ggTCGCTCCCCCCGCGGCGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                        >  1:549536/2‑99 (MQ=2)
                                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                      <  1:363515/101‑1 (MQ=255)
                                                                tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActaca                                      >  1:500958/1‑97 (MQ=2)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                        <  2:96623/101‑2 (MQ=2)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                        >  2:1426685/1‑100 (MQ=2)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCACCCCGCGGGGGCGCGGATCGAAACac                                        >  2:3513049/1‑100 (MQ=2)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgc                                      >  2:3472945/1‑97 (MQ=2)
                                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                      >  1:1551124/1‑97 (MQ=9)
                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                    >  1:3714607/1‑101 (MQ=255)
                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                    >  1:3937694/1‑95 (MQ=2)
                                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCAGCGGGGGCGTGGATCGAAActacacc                                    >  1:3552116/1‑95 (MQ=2)
                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GGGCG.GCATGGATTCATCA.ACCGC.CAT..CGAGCGTCCCTCCCCCCGCGGGGGCGTGGGACGaaaaacca                                      >  2:2610838/1‑96 (MQ=2)
                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                    <  2:3338866/101‑7 (MQ=2)
                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                    >  1:466959/1‑95 (MQ=2)
                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccg                                   <  2:1634861/101‑8 (MQ=2)
                                                                   cTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcc                                    >  1:1390635/1‑95 (MQ=9)
                                                                    tCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                  >  1:1083388/1‑93 (MQ=2)
                                                                    tCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                  <  2:658004/101‑9 (MQ=2)
                                                                    tCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                  <  1:2095289/101‑9 (MQ=2)
                                                                     ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                  <  1:1972157/101‑9 (MQ=9)
                                                                      cccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatg                                   <  2:1183173/101‑4 (MQ=2)
                                                                      cccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                 <  2:798709/101‑10 (MQ=9)
                                                                       ccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGCGGATCGAAActcggcctcga                                >  2:471760/1‑91 (MQ=2)
                                                                        cccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgcc                                 <  1:422083/101‑6 (MQ=0)
                                                                        cccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                              <  1:351593/101‑1 (MQ=255)
                                                                          cgggggggCGTGGATCGAAACG.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                            <  1:3097644/98‑1 (MQ=255)
                                                                          cgcgGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                               <  1:1934933/101‑8 (MQ=0)
                                                                          cgcgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                            <  2:2427774/101‑1 (MQ=255)
                                                                            cgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                          >  1:2586634/1‑101 (MQ=255)
                                                                             gggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                         >  1:391132/1‑101 (MQ=255)
                                                                             gggggCGAGGATCGAAACA.GCCT.GCGCG.GC.CGAA.TCGGCT.TCCGA.CTG..GGGG.GTCGCCCCCCGCGTGGTGGGGTGGCTCCTAAAATCAGCtcgctgctggt                         >  2:2217085/1‑93 (MQ=255)
                                                                               gggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                       >  2:3068961/1‑101 (MQ=255)
                                                                                 gCGTGGATCGAACCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTGGCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                     <  1:4178471/101‑1 (MQ=255)
                                                                                   gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCTCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                   >  2:1088904/1‑101 (MQ=255)
                                                                                   gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                   >  1:1051132/1‑101 (MQ=255)
                                                                                    tGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                  <  1:3361384/101‑1 (MQ=255)
                                                                                     ggATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                 >  1:641595/1‑101 (MQ=255)
                                                                                                 gCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc      >  1:1747365/1‑101 (MQ=255)
                                                                                                 gCCG.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc      <  1:1275808/101‑1 (MQ=255)
                                                                                                   cT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGc    >  2:2782294/1‑101 (MQ=255)
                                                                                                    t.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt   <  2:3257704/101‑1 (MQ=255)
                                                                                                      |                                                                                                            
CTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTC  >  NC_014034/1396840‑1397040

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG