Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,941 C→A 16.9% R275S (CGC→AGC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9410C→A16.9% 104.3 ‑0.3 ‑0.0276R275S (CGC→AGC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/22):  ref base (102/103):  total (136/140)
Fisher's exact test strand distribution p-value = 7.34e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.94e-01

Only 100 of 307 total aligned reads displayed.

TCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTC  >  NC_014034/1396841‑1397040
                                                                                                      |                                                                                                            
tCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gc                                                                                                              >  2:1145627/1‑101 (MQ=255)
      cgcgGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.Gc.c                                                                                                      <  1:4140918/101‑1 (MQ=255)
          ggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.t                                                                                                 >  1:3057096/1‑101 (MQ=255)
            ggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCg                                                                                               <  1:1826250/101‑1 (MQ=255)
               gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCt                                                                                            >  1:4043265/1‑101 (MQ=255)
                      gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.Ct                                                                                  <  2:746177/101‑1 (MQ=255)
                       aaaCCGGTCCAGAGATAGACCGCGCGTACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTg                                                                                 <  1:2547354/101‑1 (MQ=255)
                        aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..c                                                                              >  1:589947/1‑101 (MQ=255)
                             gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.Gt                                                                        >  1:691337/1‑101 (MQ=255)
                              tCCAGAGATAGACAGCGCGGACCTCCTCAGACGAGGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTc                                                                       <  1:3634689/101‑1 (MQ=255)
                                cAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGc                                                                     >  2:417198/1‑101 (MQ=255)
                                     aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GGCGCTcccc                                                                >  2:3944976/1‑101 (MQ=255)
                                       aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTcccccc                                                              <  2:4043004/101‑1 (MQ=255)
                                       aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTcccccc                                                              <  1:1970019/101‑1 (MQ=255)
                                       aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTcccccc                                                              <  1:169125/101‑1 (MQ=255)
                                        gACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCcg                                                             <  2:1761561/101‑1 (MQ=255)
                                        gACCCCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCcg                                                             <  1:756794/101‑1 (MQ=255)
                                                ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                     >  1:4180781/1‑101 (MQ=255)
                                                ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                     >  2:950168/1‑101 (MQ=255)
                                                 gccctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGt                                                    <  1:1601367/99‑1 (MQ=255)
                                                 gccccccccAGACGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCG.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGt                                                    <  1:1784856/93‑1 (MQ=37)
                                                  aCCTCCTCCGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTg                                                   <  2:432769/101‑1 (MQ=255)
                                                   ctcgcggttgcGTCGCTCCCCCCGCTGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTg                                                   <  2:1854079/91‑1 (MQ=0)
                                                   cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                  >  1:2823536/1‑101 (MQ=255)
                                                   cctcctCAGACGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                  <  1:1145721/101‑1 (MQ=255)
                                                    ggtcaacactgTCGCTCCACCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                                  <  1:836338/91‑1 (MQ=9)
                                                    attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                 <  1:290828/91‑1 (MQ=0)
                                                     ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                >  2:3202670/10‑101 (MQ=2)
                                                     ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                >  1:4226500/10‑101 (MQ=2)
                                                     tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                >  1:1018959/1‑101 (MQ=255)
                                                      ccccgcccGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTgg                                                  <  1:268214/94‑1 (MQ=0)
                                                       cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                               <  1:1343515/95‑1 (MQ=2)
                                                       cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                               >  2:2627926/7‑101 (MQ=2)
                                                       caactctgTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                               <  1:2149614/94‑1 (MQ=9)
                                                        tcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                             >  1:3723826/1‑101 (MQ=255)
                                                        ccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                <  2:1557810/96‑1 (MQ=2)
                                                        aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                              <  2:3375370/95‑1 (MQ=9)
                                                         gttgcggcGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                             >  1:1921921/8‑101 (MQ=2)
                                                         gttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                             <  1:2234409/97‑1 (MQ=2)
                                                         ccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                            <  2:2439711/96‑1 (MQ=2)
                                                         ccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                            <  2:2243330/96‑1 (MQ=2)
                                                         ccccggTCGCTCACCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                            >  1:2289869/6‑101 (MQ=2)
                                                         cagccgtgccTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                            <  2:3282143/92‑1 (MQ=255)
                                                         acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                             >  2:3598272/6‑101 (MQ=9)
                                                          gaccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                           >  2:1579462/4‑101 (MQ=255)
                                                            cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                         >  2:2687360/3‑100 (MQ=2)
                                                            cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                         <  2:2016190/99‑2 (MQ=2)
                                                            cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                         <  2:594928/99‑2 (MQ=2)
                                                            cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAct                                         >  2:1225835/3‑100 (MQ=2)
                                                             ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                        >  2:2609413/2‑99 (MQ=2)
                                                             ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                        >  1:1722476/2‑99 (MQ=2)
                                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                       <  2:3711751/101‑1 (MQ=255)
                                                               tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcg                                       >  1:991203/1‑98 (MQ=9)
                                                               tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcg                                       <  1:3387187/101‑4 (MQ=9)
                                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                        <  2:96623/101‑2 (MQ=2)
                                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCACCCCGCGGGGGCGCGGATCGAAACac                                        >  2:3513049/1‑100 (MQ=2)
                                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgc                                      >  2:3472945/1‑97 (MQ=2)
                                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacac                                     >  1:3934293/1‑96 (MQ=2)
                                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                      >  1:1551124/1‑97 (MQ=9)
                                                                cGCCCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgc                                      <  2:2850897/101‑5 (MQ=2)
                                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaaaccc                                       >  2:3421640/1‑97 (MQ=2)
                                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                     >  2:3732868/1‑96 (MQ=2)
                                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAcccgcc                                     >  2:2928340/1‑96 (MQ=2)
                                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                    >  2:1309375/1‑95 (MQ=2)
                                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                     >  2:3038203/1‑96 (MQ=9)
                                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                     >  1:3905791/1‑96 (MQ=9)
                                                                  cTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                    <  2:3338866/101‑7 (MQ=2)
                                                                  cTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcc                                    >  1:1390635/1‑95 (MQ=9)
                                                                  cTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTC.GCCCGCGC.CGCG.CCCGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAActcggcc                                    >  1:3827762/1‑95 (MQ=2)
                                                                   tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                  >  2:2547856/1‑101 (MQ=255)
                                                                   tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                  >  1:287457/1‑101 (MQ=255)
                                                                   tCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                  <  1:2095289/101‑9 (MQ=2)
                                                                   tCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                  <  2:658004/101‑9 (MQ=2)
                                                                   tCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                  >  1:1083388/1‑93 (MQ=2)
                                                                    nccncnGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctn                                  <  1:894724/100‑9 (MQ=9)
                                                                     cccccGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAcccgccacgt                                 >  2:1664950/1‑92 (MQ=2)
                                                                     cccccGCGGGGGCGTGGATCGAAACA.CCCG.GCGCC.GC.AGCG.AGTGCG.TCTTGA.ACA..GGAT.GTCGCTCCCCCCGCAGGGGCGTGGATCGAAActatgctggc                                 >  1:3551874/1‑92 (MQ=2)
                                                                     cccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                 <  2:798709/101‑10 (MQ=9)
                                                                     cccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                 <  2:1390535/101‑10 (MQ=9)
                                                                     cccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                 >  1:3950620/1‑92 (MQ=9)
                                                                      ccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGCGGATCGAAActcggcctcga                                >  2:471760/1‑91 (MQ=2)
                                                                       cccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGAGCtcgtcg                              >  1:4113510/1‑101 (MQ=255)
                                                                           cgGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                          >  1:3257945/1‑101 (MQ=255)
                                                                            gggggCGGGGAACGAAACA.GCCG.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                         <  2:3097959/101‑1 (MQ=255)
                                                                                gCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                     <  2:1797123/101‑1 (MQ=255)
                                                                                  gTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCg                   >  1:1051132/1‑101 (MQ=255)
                                                                                     gATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc                <  2:2067419/101‑1 (MQ=255)
                                                                                      aTCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg               <  2:1077494/101‑1 (MQ=255)
                                                                                      aTCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg               <  2:3891940/101‑1 (MQ=255)
                                                                                        cGAAGCA.GCCG.GCGCG.GC.CGCA.TCTGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGAt             <  2:2401862/101‑1 (MQ=255)
                                                                                         gAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCTCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTCGTCAGCCGGACGATg            >  1:476185/1‑101 (MQ=255)
                                                                                            aCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc         <  1:3699062/101‑1 (MQ=255)
                                                                                             cA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGGCAGCCGGACGATGTCCg        >  1:525258/1‑101 (MQ=255)
                                                                                                gCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc      <  2:3251375/101‑1 (MQ=255)
                                                                                                gCCT.GCGCG.GC.CGCA.TCGCCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc      <  2:1934989/101‑1 (MQ=255)
                                                                                                gCCG.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc      <  1:1275808/101‑1 (MQ=255)
                                                                                                  cT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGc    >  2:2782294/1‑101 (MQ=255)
                                                                                                   t.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt   <  1:3125621/101‑1 (MQ=255)
                                                                                                   t.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt   <  2:1108690/101‑1 (MQ=255)
                                                                                                     gcgcG.GC.CGCA.GCGGCG.TC.CGC.CTG..CGTG.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTc  <  1:3296079/101‑1 (MQ=255)
                                                                                                      |                                                                                                            
TCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTC  >  NC_014034/1396841‑1397040

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG