Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,947 C→G 17.5% R277G (CGC→GGT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9470C→G17.5% 100.4 ‑0.1 ‑0.9283R277G (CGC→GGC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/22):  ref base (94/101):  total (135/148)
Fisher's exact test strand distribution p-value = 8.65e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.13e-01

Only 100 of 305 total aligned reads displayed.

CGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGC  >  NC_014034/1396847‑1397047
                                                                                                          |                                                                                                          
cgcgGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.Gc.c                                                                                                            <  1:4140918/101‑1 (MQ=255)
   gggggCGTGGATCGAACCCGGTCCAGACATAGACCGCGCGTCCCTCCTCATCCGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCa                                                                                                         <  1:4201175/101‑1 (MQ=37)
    ggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.t                                                                                                       <  1:1993735/101‑1 (MQ=255)
     gggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.Tc                                                                                                      <  2:1657081/101‑1 (MQ=255)
        cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGc                                                                                                   >  1:1340677/1‑101 (MQ=255)
        cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGc                                                                                                   <  1:1495139/101‑1 (MQ=255)
         gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCt                                                                                                  >  1:3098189/1‑101 (MQ=255)
          tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGTATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCt.t                                                                                                <  2:3478967/101‑1 (MQ=255)
             aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.Tccc                                                                                             >  1:1108784/1‑100 (MQ=255)
               cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGc.c                                                                                          <  2:4030411/101‑1 (MQ=255)
                gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                         <  1:1369072/101‑1 (MQ=255)
                gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                         <  2:746177/101‑1 (MQ=255)
                  acccGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..c                                                                                     <  2:3899835/99‑1 (MQ=255)
                  aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..c                                                                                     >  1:589947/1‑101 (MQ=255)
                      ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CCG..CGTg.g                                                                                >  1:2891896/1‑101 (MQ=255)
                       gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.Gt                                                                               >  1:691337/1‑101 (MQ=255)
                                tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                                      >  1:2162195/1‑101 (MQ=255)
                                tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                                      >  1:1797242/1‑101 (MQ=255)
                                tAGACCGCGCGGACCTCCTCAGACGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                                      <  2:2766031/101‑1 (MQ=255)
                                 aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                                     <  1:2825086/101‑1 (MQ=255)
                                  gACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCcg                                                                    <  2:1761561/101‑1 (MQ=255)
                                  gACCCCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCcg                                                                    <  1:756794/101‑1 (MQ=255)
                                     cgcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCgg                                                                 <  1:1909163/101‑1 (MQ=255)
                                          ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                            >  2:950168/1‑101 (MQ=255)
                                          ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                            <  1:1080464/101‑1 (MQ=255)
                                             ctcgcggttgcGTCGCTCCCCCCGCTGGGGCGTGGATCGAAACATGG.CTAT.GGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTg                                                          <  2:1854079/91‑1 (MQ=0)
                                              ggtcaacactgTCGCTCCACCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                                         <  1:836338/91‑1 (MQ=9)
                                              ctcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                        <  1:2127599/101‑1 (MQ=255)
                                               tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                       >  1:1018959/1‑101 (MQ=255)
                                                tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.ACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                      <  1:2628118/93‑1 (MQ=2)
                                                tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.ACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                      <  2:2393398/93‑1 (MQ=2)
                                                tcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                       >  2:3599946/9‑101 (MQ=9)
                                                gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                       <  2:1243074/94‑1 (MQ=2)
                                                 ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.ACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                     <  2:3232107/94‑1 (MQ=2)
                                                 cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                      <  2:3268080/95‑1 (MQ=2)
                                                  tcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                    >  1:3723826/1‑101 (MQ=255)
                                                  ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                     >  2:412144/6‑101 (MQ=2)
                                                  ccgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.G.CGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                       <  2:1557810/96‑1 (MQ=2)
                                                  aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                     <  2:3375370/95‑1 (MQ=9)
                                                   cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                   >  2:1314058/1‑101 (MQ=255)
                                                   cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                   >  1:3035506/1‑101 (MQ=255)
                                                    gaccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  >  2:1579462/4‑101 (MQ=255)
                                                    cccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.ACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  <  2:2648408/97‑1 (MQ=2)
                                                     tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                  >  2:2429961/3‑101 (MQ=2)
                                                      cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.ACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                <  2:2016190/99‑2 (MQ=2)
                                                      cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.ACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                <  2:594928/99‑2 (MQ=2)
                                                      aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                >  2:3712400/1‑101 (MQ=255)
                                                       tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                <  2:2848824/100‑2 (MQ=9)
                                                       tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                >  1:1676223/2‑100 (MQ=9)
                                                        gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.ACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActac                                              <  2:2528041/101‑4 (MQ=2)
                                                        gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                              <  1:3594269/101‑1 (MQ=255)
                                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccg                                              >  2:437598/1‑98 (MQ=2)
                                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.ACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActaca                                             <  1:2235987/101‑5 (MQ=2)
                                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcg                                              >  1:991203/1‑98 (MQ=9)
                                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcg                                              <  1:3387187/101‑4 (MQ=9)
                                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.G.CGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                               <  2:96623/101‑2 (MQ=2)
                                                          cGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.ACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacac                                            >  1:3934293/1‑96 (MQ=2)
                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.G.CGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaaaccc                                              >  2:3421640/1‑97 (MQ=2)
                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.ACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                           >  2:4172414/1‑95 (MQ=2)
                                                           gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                            >  1:3905791/1‑96 (MQ=9)
                                                            cTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                           <  2:3338866/101‑7 (MQ=2)
                                                            cTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                           <  2:3087523/101‑7 (MQ=2)
                                                            cTCCCCCCGCGGGGGCGTGGATCGAAACA.CCAAT.GCCGAA.GC.CAAG.GCCGCA.GCAGC.GGC..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAACGAAACaaaaa                                            >  1:1778079/1‑97 (MQ=2)
                                                            cTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcc                                           >  2:501510/1‑95 (MQ=9)
                                                            cTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TC.G.CCCGCGC.CGCG.CCCGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAActcggcc                                           >  1:3827762/1‑95 (MQ=2)
                                                             tCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                         >  1:1045238/1‑101 (MQ=255)
                                                             tCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                         >  1:1948322/1‑101 (MQ=255)
                                                             tCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.ACC...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                         <  2:658004/101‑9 (MQ=2)
                                                             tCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                          >  1:3196182/1‑94 (MQ=9)
                                                             cccccccGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                          <  1:1246585/100‑8 (MQ=9)
                                                              nccncnGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctn                                         <  1:894724/100‑9 (MQ=9)
                                                              ccccccGCGGGGGCGTGGATCGAAACT.GC.GT.G.CGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                           >  1:1720171/1‑99 (MQ=2)
                                                              ccccccGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                         >  1:4230925/1‑93 (MQ=9)
                                                              ccccccGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                         <  1:1551679/101‑9 (MQ=9)
                                                               cccccGCGGGGGCGTGGATCGAAACT.GC.GT.G.CGCG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatg                                          <  2:1183173/101‑4 (MQ=2)
                                                               cccccGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                        <  2:1390535/101‑10 (MQ=9)
                                                               cccccGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                        <  2:798709/101‑10 (MQ=9)
                                                               cccccGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                        >  1:3950620/1‑92 (MQ=9)
                                                                ccccGCGGGGGCGTGGATCGAAACATGG.CTAT.GGCG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacgtc                                       >  1:49656/1‑91 (MQ=2)
                                                                 cccGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGAGCtcgtcg                                     >  1:4113510/1‑101 (MQ=255)
                                                                 cccGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                                     >  1:3518724/1‑101 (MQ=255)
                                                                   cgcgGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACATCAGCTCGTCGtt                                   <  1:2400420/101‑1 (MQ=255)
                                                                     cgGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                                 <  2:2891677/101‑1 (MQ=255)
                                                                      gggggCGAGGATCGAAACA.GC.CT.G.CGCG.GC.CGAA.TCGGCT.TCCGA.CTG..GGGG.GTCGCCCCCCGCGTGGTGGGGTGGCTCCTAAAATCAGCtcgctgctggt                                >  2:2217085/1‑93 (MQ=255)
                                                                        gggCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                              <  2:3165396/101‑1 (MQ=255)
                                                                          gCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                            <  2:2961625/101‑1 (MQ=255)
                                                                              ggATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                        >  2:2612140/1‑101 (MQ=255)
                                                                               gATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc                       <  2:2067419/101‑1 (MQ=255)
                                                                                aTCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg                      <  2:1077494/101‑1 (MQ=255)
                                                                                aTCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg                      <  2:2586443/101‑1 (MQ=255)
                                                                                      aCA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc                <  1:3699062/101‑1 (MQ=255)
                                                                                       cA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGGCGATGTCCg               >  2:381142/1‑101 (MQ=255)
                                                                                       cA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTACg               <  2:3518471/101‑1 (MQ=255)
                                                                                       cA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGGCAGCCGGACGATGTCCg               >  1:525258/1‑101 (MQ=255)
                                                                                          gC.CT.G.CGCG.GC.CGCA.TCGCCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc             <  2:1934989/101‑1 (MQ=255)
                                                                                              t.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt          <  2:1108690/101‑1 (MQ=255)
                                                                                                g.cgcG.GC.CGCA.GCGGCG.TCCGC.CTG..CGTG.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTc         <  1:3296079/101‑1 (MQ=255)
                                                                                                     g.gC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccccc     >  2:1825463/1‑101 (MQ=255)
                                                                                                        c.cGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcg   >  1:2729697/1‑101 (MQ=255)
                                                                                                        c.cGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCCGACGATGTCCGTCGCTcccccc  >  1:4079993/1‑100 (MQ=255)
                                                                                                          |                                                                                                          
CGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGC  >  NC_014034/1396847‑1397047

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG