Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,949 C→T 10.3% R277R (CGC→GGT RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9490C→T10.3% 60.8 0.0 ‑0.0287R277R (CGC→CGTRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (14/15):  ref base (118/123):  total (139/148)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.90e-01

Only 100 of 307 total aligned reads displayed.

GGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGG  >  NC_014034/1396850‑1397049
                                                                                                        |                                                                                                           
gggggCGTGGATCGAACCCGGTCCAGACATAGACCGCGCGTCCCTCCTCATCCGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCa                                                                                                            <  1:4201175/101‑1 (MQ=37)
  gggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.Tc                                                                                                         <  2:1657081/101‑1 (MQ=255)
   ggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCg                                                                                                        <  1:1826250/101‑1 (MQ=255)
     cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGc                                                                                                      >  1:1340677/1‑101 (MQ=255)
      gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCt                                                                                                     >  1:3098189/1‑101 (MQ=255)
          aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.Tc.cc                                                                                               >  1:1108784/1‑100 (MQ=255)
           tCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GGCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGc                                                                                              >  2:3372974/1‑101 (MQ=255)
            cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGc.c                                                                                            <  2:4030411/101‑1 (MQ=255)
             gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.Ct                                                                                           <  2:746177/101‑1 (MQ=255)
             gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.Ct                                                                                           <  1:1369072/101‑1 (MQ=255)
             gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.Ct                                                                                           <  2:1847080/101‑1 (MQ=255)
               acccGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..c                                                                                       <  2:3899835/99‑1 (MQ=255)
               aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..c                                                                                       <  2:2637928/101‑1 (MQ=255)
                   ggTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CCG..CGTg.g                                                                                  >  1:2891896/1‑101 (MQ=255)
                     gccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTc                                                                                <  1:981186/100‑1 (MQ=255)
                             tAGACCGCGCGGACCTCCTCAGACGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTccccc                                                                        <  2:2766031/101‑1 (MQ=255)
                              aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTcccccc                                                                       <  1:2825086/101‑1 (MQ=255)
                              aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTcccccc                                                                       <  1:169125/101‑1 (MQ=255)
                               gACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCcg                                                                      <  2:1761561/101‑1 (MQ=255)
                                   gcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCggg                                                                  >  2:2183098/1‑101 (MQ=255)
                                       ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                              >  2:950168/1‑101 (MQ=255)
                                       ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                              >  2:3419439/1‑101 (MQ=255)
                                       ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                              <  1:1080464/101‑1 (MQ=255)
                                        gccccccccAGACGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCG.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGt                                                             <  1:1784856/93‑1 (MQ=37)
                                            ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                         <  1:777828/92‑1 (MQ=2)
                                            tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                         >  1:1018959/1‑101 (MQ=255)
                                            tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                         >  2:1578004/1‑101 (MQ=255)
                                             tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  1:875586/93‑1 (MQ=2)
                                             tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  1:2628118/93‑1 (MQ=2)
                                              ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                       <  2:3232107/94‑1 (MQ=2)
                                              cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  2:3268080/95‑1 (MQ=2)
                                              caactctgTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  1:2149614/94‑1 (MQ=9)
                                              caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  2:2465886/94‑1 (MQ=9)
                                               tcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                      >  1:3723826/1‑101 (MQ=255)
                                               ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                       >  2:412144/6‑101 (MQ=2)
                                               ggttgcGTCGCTCCACCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                       <  2:1072222/96‑1 (MQ=2)
                                               aagactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                       >  1:2420254/7‑101 (MQ=9)
                                                gttgcggcGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                      >  1:1921921/8‑101 (MQ=2)
                                                ccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     >  2:1141088/6‑101 (MQ=2)
                                                cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     >  1:3035506/1‑101 (MQ=255)
                                                acactggcGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCCC.GC.CGCG.CCAGATCTT.TGT.CTG..GGCG.GGGGCTCCCCCCGCGGGGGCGTGCATCGa                                                      <  2:1965481/94‑1 (MQ=2)
                                                acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                      >  2:3598272/6‑101 (MQ=9)
                                                   gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                   <  2:2561397/100‑1 (MQ=2)
                                                   cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                  <  2:594928/99‑2 (MQ=2)
                                                   cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAct                                                  >  2:1225835/3‑100 (MQ=2)
                                                   aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                  >  2:3712400/1‑101 (MQ=255)
                                                    tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                  <  2:2848824/100‑2 (MQ=9)
                                                    tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                  <  2:2383205/100‑2 (MQ=9)
                                                    ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                 >  1:1722476/2‑99 (MQ=2)
                                                    ggTCGCTCCCCCCGCGGCGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                 >  1:549536/2‑99 (MQ=2)
                                                    cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                 >  1:3451065/1‑101 (MQ=255)
                                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                   >  2:1369533/1‑101 (MQ=2)
                                                     gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                <  1:3594269/101‑1 (MQ=255)
                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccg                                                >  2:437598/1‑98 (MQ=2)
                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGCGGATCGAAACATCa                                               >  2:1906603/1‑101 (MQ=255)
                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActaca                                               <  1:7030/101‑5 (MQ=2)
                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActaca                                               >  1:500958/1‑97 (MQ=2)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                 <  2:96623/101‑2 (MQ=2)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                 >  2:1426685/1‑100 (MQ=2)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCACCCCGCGGGGGCGCGGATCGAAACac                                                 >  2:3513049/1‑100 (MQ=2)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgc                                               >  2:3472945/1‑97 (MQ=2)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacac                                              >  1:3934293/1‑96 (MQ=2)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                               >  1:1551124/1‑97 (MQ=9)
                                                       cGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAg                                              <  2:3315692/101‑1 (MQ=255)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                             <  1:417249/101‑1 (MQ=255)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                             >  2:4172414/1‑95 (MQ=2)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                             >  2:1309375/1‑95 (MQ=2)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                              >  1:3905791/1‑96 (MQ=9)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GGG.CG.GCATGGATTCATCA.AC.CGC.CAT..CGAGCGTCCCTCCCCCCGCGGGGGCGTGGGACGaaaaacca                                               >  2:2610838/1‑96 (MQ=2)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                             <  2:3087523/101‑7 (MQ=2)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcc                                             >  2:501510/1‑95 (MQ=9)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTC.GCC.CGCGC.CGCG.CCCGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAActcggcc                                             >  1:3827762/1‑95 (MQ=2)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                           >  1:1045238/1‑101 (MQ=255)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                           <  2:658004/101‑9 (MQ=2)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                           <  1:2095289/101‑9 (MQ=2)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                            >  1:3196182/1‑94 (MQ=9)
                                                          cccccccGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                           <  2:1102975/100‑9 (MQ=2)
                                                          cccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                            <  1:1246585/100‑8 (MQ=9)
                                                           ccccccGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                          <  1:2272527/101‑10 (MQ=2)
                                                           ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                           >  1:4230925/1‑93 (MQ=9)
                                                           ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                           <  1:1972157/101‑9 (MQ=9)
                                                            cccccGCGGGGGCGTGGATCGAAACA.CCCG.GCG.CC.GC.AGCG.AGTGCG.TCTTGA.ACA..GGAT.GTCGCTCCCCCCGCAGGGGCGTGGATCGAAActatgctggc                                          >  1:3551874/1‑92 (MQ=2)
                                                            cccccGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                          <  2:798709/101‑10 (MQ=9)
                                                            cccccGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                          >  1:3950620/1‑92 (MQ=9)
                                                              cccGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGAGCtcgtcg                                       >  1:4113510/1‑101 (MQ=255)
                                                                cgcgGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                                     <  2:2427774/101‑1 (MQ=255)
                                                                 gcgGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGTCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTg                                    <  2:206652/101‑1 (MQ=255)
                                                                  cgGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                                   <  2:2891677/101‑1 (MQ=255)
                                                                     gggCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                                <  2:3165396/101‑1 (MQ=255)
                                                                       gCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                              <  2:2961625/101‑1 (MQ=255)
                                                                       gCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                              <  2:1797123/101‑1 (MQ=255)
                                                                           ggATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                          >  1:641595/1‑101 (MQ=255)
                                                                                   aCA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc                  <  1:3699062/101‑1 (MQ=255)
                                                                                    cA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGGCGATGTCCg                 >  2:381142/1‑101 (MQ=255)
                                                                                            gcg.cG.GC.CGCA.GCGGCG.TC.CGC.CTG..CGTG.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTc           <  1:3296079/101‑1 (MQ=255)
                                                                                                   gC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccccc      <  1:1410445/101‑1 (MQ=255)
                                                                                                    c.cGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcg     >  1:2729697/1‑101 (MQ=255)
                                                                                                    c.cGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCCGACGATGTCCGTCGCTccccccc     >  1:4079993/1‑100 (MQ=255)
                                                                                                       gCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg   >  1:1586632/1‑101 (MQ=255)
                                                                                                        cA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCgg  <  1:2183243/101‑1 (MQ=255)
                                                                                                        |                                                                                                           
GGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGG  >  NC_014034/1396850‑1397049

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG