Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,953 G→A 16.3% G279S (GGC→ATA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9530G→A16.3% 123.1 ‑0.5 ‑0.6283G279S (GGC→AGC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (21/25):  ref base (117/118):  total (139/144)
Fisher's exact test strand distribution p-value = 6.32e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.44e-01

Only 100 of 305 total aligned reads displayed.

GGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1396853‑1397053
                                                                                                          |                                                                                                         
ggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCg                                                                                                           <  1:1826250/101‑1 (MQ=255)
  cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGc                                                                                                         >  1:1340677/1‑101 (MQ=255)
   gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCt                                                                                                        >  2:3125390/1‑101 (MQ=255)
   gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCt                                                                                                        >  1:4043265/1‑101 (MQ=255)
   gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCt                                                                                                        >  1:3098189/1‑101 (MQ=255)
   gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCt                                                                                                        >  2:1784737/1‑101 (MQ=255)
    tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCt.t                                                                                                      >  2:639601/1‑101 (MQ=255)
       aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.Tccc                                                                                                   >  1:1108784/1‑100 (MQ=255)
         cGAAACCGGTCCAGAGATAGACCGCGCGGACGTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGc.c                                                                                                >  1:3749821/1‑101 (MQ=255)
         cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGc.c                                                                                                <  2:2861881/101‑1 (MQ=255)
          gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                               <  1:1369072/101‑1 (MQ=255)
          gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                               <  2:1847080/101‑1 (MQ=255)
            aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..c                                                                                           <  2:2637928/101‑1 (MQ=255)
            aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..c                                                                                           <  2:2734588/101‑1 (MQ=255)
                  gccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTc                                                                                    <  1:981186/100‑1 (MQ=255)
                    cAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GGCGc                                                                                  >  2:4038710/1‑101 (MQ=255)
                     agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCt                                                                                 >  2:3899840/1‑101 (MQ=255)
                      gagaTAGACCGCTCGGACCTCCTCAGACGCCGCTCCCCCTGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGG.CTG..CGTG.GTCGCTc                                                                                >  2:4178209/1‑101 (MQ=38)
                        gaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccc                                                                              <  1:2725962/101‑1 (MQ=255)
                         aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccc                                                                             <  2:3502560/101‑1 (MQ=255)
                         aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GGCGCTcccc                                                                             >  2:3204558/1‑101 (MQ=255)
                         aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GCCGCTcccc                                                                             >  2:3472049/1‑101 (MQ=255)
                          tAGCCCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                                            <  2:824837/101‑1 (MQ=255)
                          tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                                            >  2:4131773/1‑101 (MQ=255)
                          tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                                            >  1:2162195/1‑101 (MQ=255)
                           aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                                           <  1:169125/101‑1 (MQ=255)
                               cgcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCgg                                                                       <  2:701552/101‑1 (MQ=255)
                                gcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCggg                                                                      >  2:2183098/1‑101 (MQ=255)
                                    ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                                  <  1:1080464/101‑1 (MQ=255)
                                     gccccccccAGACGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCG.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGt                                                                 <  1:1784856/93‑1 (MQ=37)
                                       cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                               >  1:2823536/1‑101 (MQ=255)
                                        tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                               <  2:2948618/92‑1 (MQ=0)
                                        ctcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                              <  1:2127599/101‑1 (MQ=255)
                                        ctcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                              <  2:281490/101‑1 (MQ=255)
                                        attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                              <  1:290828/91‑1 (MQ=0)
                                         ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                             <  1:777828/92‑1 (MQ=2)
                                         tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                             >  2:1578004/1‑101 (MQ=255)
                                         tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                             >  1:1018959/1‑101 (MQ=255)
                                          tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                            <  1:875586/93‑1 (MQ=2)
                                           ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                           <  2:1612696/94‑1 (MQ=2)
                                           caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                            <  2:2465886/94‑1 (MQ=9)
                                            ggttgcGTCGCTCCACCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                           <  2:1072222/96‑1 (MQ=2)
                                            aacactgTCGCTCCCCCCGCGGGGGGGTGGATCGAAAC..GCGG.GCC.CGCGC.CGCG.CCGGATCTTTGT.CGG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAttg                                                           >  1:4135029/7‑99 (MQ=2)
                                             gttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                          <  1:2234409/97‑1 (MQ=2)
                                             gttgcGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                          <  1:4090654/97‑1 (MQ=2)
                                             cgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                            <  2:764498/97‑1 (MQ=2)
                                             ccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                         >  2:1141088/6‑101 (MQ=2)
                                             ccccggTCGCTCACCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                         >  1:2289869/6‑101 (MQ=2)
                                             cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                         <  2:3867013/101‑1 (MQ=255)
                                             acactggcGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCCC.GC.CGCG.CCAGATCTTTGT.CTG..GGCG.GGGGCTCCCCCCGCGGGGGCGTGCATCGa                                                          <  2:1965481/94‑1 (MQ=2)
                                              aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                        >  2:1681531/1‑101 (MQ=255)
                                                gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                       <  2:2561397/100‑1 (MQ=2)
                                                gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                       <  1:3900838/100‑1 (MQ=2)
                                                cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAct                                                      >  2:1225835/3‑100 (MQ=2)
                                                 tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                      <  2:2383205/100‑2 (MQ=9)
                                                 tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                      <  2:3726950/100‑2 (MQ=9)
                                                 ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                     >  1:1722476/2‑99 (MQ=2)
                                                 ggTCGCTCCCCCCGCGGCGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                     >  1:549536/2‑99 (MQ=2)
                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                       >  2:1369533/1‑101 (MQ=2)
                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                    <  2:3711751/101‑1 (MQ=255)
                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                   <  1:363515/101‑1 (MQ=255)
                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGCGGATCGAAACATCa                                                   >  2:1906603/1‑101 (MQ=255)
                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActaca                                                   <  1:7030/101‑5 (MQ=2)
                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActaca                                                   >  1:500958/1‑97 (MQ=2)
                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcg                                                    <  1:3387187/101‑4 (MQ=9)
                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                     <  2:444527/101‑2 (MQ=2)
                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                     <  2:3356722/101‑2 (MQ=2)
                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                     <  2:96623/101‑2 (MQ=2)
                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                     >  2:1426685/1‑100 (MQ=2)
                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcgg                                                   >  1:1551124/1‑97 (MQ=9)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                                 >  1:3714607/1‑101 (MQ=255)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACATCGGc                                                 >  2:3566385/1‑101 (MQ=255)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                                 >  2:1309375/1‑95 (MQ=2)
                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GGG.CG.GCATGGATTCATCA.ACCGC.CAT..CGAGCGTCCCTCCCCCCGCGGGGGCGTGGGACGaaaaacca                                                   >  2:2610838/1‑96 (MQ=2)
                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                                 >  1:466959/1‑95 (MQ=2)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                               >  1:1045238/1‑101 (MQ=255)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                               >  1:287457/1‑101 (MQ=255)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                >  2:505930/1‑94 (MQ=9)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                >  1:3920103/1‑94 (MQ=9)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                >  1:3196182/1‑94 (MQ=9)
                                                       cccccccGCGGGGGCGTGGATCGAAACA..ACT.GAC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                               <  2:1102975/100‑9 (MQ=2)
                                                       cccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                <  1:1246585/100‑8 (MQ=9)
                                                        ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                               <  1:1972157/101‑9 (MQ=9)
                                                         cccccGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgt                                             <  2:3391650/101‑1 (MQ=255)
                                                           cccGCGGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgcc                                              <  1:422083/101‑6 (MQ=0)
                                                           cccGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                                           >  1:3518724/1‑101 (MQ=255)
                                                             cgcgGGGGCGTGGATCGAAACT.GCGT.GCG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                                            <  1:1934933/101‑8 (MQ=0)
                                                             cgcgGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                                         <  2:2427774/101‑1 (MQ=255)
                                                              gcgGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGTCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTg                                        <  2:206652/101‑1 (MQ=255)
                                                               cgGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                                       >  1:2586634/1‑101 (MQ=255)
                                                                  gggCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                                    >  2:3068961/1‑101 (MQ=255)
                                                                        ggATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                              >  1:641595/1‑101 (MQ=255)
                                                                         gATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc                             <  2:3314361/101‑1 (MQ=255)
                                                                                 cA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg                     <  1:3945236/101‑1 (MQ=255)
                                                                                       t.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt                <  2:3257704/101‑1 (MQ=255)
                                                                                         gcg.cG.GC.CGCA.GCGGCG.TCCGC.CTG..CGTG.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTc               <  1:3296079/101‑1 (MQ=255)
                                                                                                gC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTcccccc          <  1:1410445/101‑1 (MQ=255)
                                                                                                    gCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGGAACATAAGCTCGTCGTTGGTCAGCAGGACGATGTCCGTCGCTCCCCCcgcg       >  1:3555133/1‑101 (MQ=255)
                                                                                                    gCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCcgcg       >  1:1586632/1‑101 (MQ=255)
                                                                                                          ggCT.TCCGC.GTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGc  <  2:952332/101‑1 (MQ=255)
                                                                                                          |                                                                                                         
GGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1396853‑1397053

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG