Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,954 G→T 6.3% G279V (GGC→ATA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9540G→T 6.3% 32.6 ‑0.8 ‑0.4283G279V (GGC→GTC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/11):  ref base (132/133):  total (139/144)
Fisher's exact test strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.05e-01

Only 100 of 305 total aligned reads displayed.

CGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCG  >  NC_014034/1396855‑1397054
                                                                                                           |                                                                                                         
cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGc                                                                                                          >  1:1340677/1‑101 (MQ=255)
 gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCt                                                                                                         >  1:3098189/1‑101 (MQ=255)
 gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCt                                                                                                         >  2:1784737/1‑101 (MQ=255)
 gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCt                                                                                                         >  2:3125390/1‑101 (MQ=255)
 gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCt                                                                                                         >  1:4043265/1‑101 (MQ=255)
  tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCt.t                                                                                                       >  2:639601/1‑101 (MQ=255)
     aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.Tccc                                                                                                    >  1:1108784/1‑100 (MQ=255)
       cGAAACCGGTCCAGAGATAGACCGCGCGGACGTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGc.c                                                                                                 >  1:3749821/1‑101 (MQ=255)
       cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGc.c                                                                                                 <  2:2861881/101‑1 (MQ=255)
        gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.Ct                                                                                                <  2:1847080/101‑1 (MQ=255)
          aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..c                                                                                            <  2:2637928/101‑1 (MQ=255)
          aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..c                                                                                            <  2:2734588/101‑1 (MQ=255)
                gccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTc                                                                                     <  1:981186/100‑1 (MQ=255)
                  cAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GGCGc                                                                                   >  2:4038710/1‑101 (MQ=255)
                   agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCt                                                                                  >  2:3899840/1‑101 (MQ=255)
                    gagaTAGACCGCTCGGACCTCCTCAGACGCCGCTCCCCCTGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGG.CTG..CGTG.GTCGCTc                                                                                 >  2:4178209/1‑101 (MQ=38)
                      gaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccc                                                                               <  1:2725962/101‑1 (MQ=255)
                       aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTcccc                                                                              <  2:3502560/101‑1 (MQ=255)
                       aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GGCGCTcccc                                                                              >  2:3204558/1‑101 (MQ=255)
                       aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GCCGCTcccc                                                                              >  2:3472049/1‑101 (MQ=255)
                        tAGCCCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                                             <  2:824837/101‑1 (MQ=255)
                        tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                                             >  1:2162195/1‑101 (MQ=255)
                        tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                                             >  2:4131773/1‑101 (MQ=255)
                        tAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTccccc                                                                             >  1:1797242/1‑101 (MQ=255)
                             cgcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCgg                                                                        <  2:701552/101‑1 (MQ=255)
                              gcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCggg                                                                       >  2:2183098/1‑101 (MQ=255)
                                  ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                                   <  1:1080464/101‑1 (MQ=255)
                                     cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                >  1:2823536/1‑101 (MQ=255)
                                      tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                <  2:2948618/92‑1 (MQ=0)
                                      ctcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                               <  2:281490/101‑1 (MQ=255)
                                      ctcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                               <  1:2127599/101‑1 (MQ=255)
                                      attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.AC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                               <  1:290828/91‑1 (MQ=0)
                                       ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.AC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                              <  1:777828/92‑1 (MQ=2)
                                       tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                              >  2:1578004/1‑101 (MQ=255)
                                       tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                              >  1:1018959/1‑101 (MQ=255)
                                        tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.AC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                             <  1:875586/93‑1 (MQ=2)
                                         ggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.AC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                            <  2:1612696/94‑1 (MQ=2)
                                         caacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                             <  2:2465886/94‑1 (MQ=9)
                                          ggttgcGTCGCTCCACCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                            <  2:1072222/96‑1 (MQ=2)
                                          aacactgTCGCTCCCCCCGCGGGGGGGTGGATCGAAAC..GC.GG.G.CC.CGCGC.CGCG.CCGGATCTTTGT.CGG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAttg                                                            >  1:4135029/7‑99 (MQ=2)
                                           gttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                           <  1:2234409/97‑1 (MQ=2)
                                           gttgcGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                           <  1:4090654/97‑1 (MQ=2)
                                           cgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.G.CG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                             <  2:764498/97‑1 (MQ=2)
                                           ccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.AC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                          >  2:1141088/6‑101 (MQ=2)
                                           ccccggTCGCTCACCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.AC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                          >  1:2289869/6‑101 (MQ=2)
                                           cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                          <  2:3867013/101‑1 (MQ=255)
                                           acactggcGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCCC.GC.CGCG.CCAGATCTTTGT.CTG..GGCG.GGGGCTCCCCCCGCGGGGGCGTGCATCGa                                                           <  2:1965481/94‑1 (MQ=2)
                                            aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                         >  2:1681531/1‑101 (MQ=255)
                                              gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                        <  2:2561397/100‑1 (MQ=2)
                                              gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                        <  1:3900838/100‑1 (MQ=2)
                                              cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.AC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAct                                                       >  2:1225835/3‑100 (MQ=2)
                                               tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                       <  2:2383205/100‑2 (MQ=9)
                                               tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                       >  1:1676223/2‑100 (MQ=9)
                                               tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                       <  2:3726950/100‑2 (MQ=9)
                                               ggTCGCTCCCCCCGCGGCGGCGTGGATCGAAACA..A.CT.G.AC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                      >  1:549536/2‑99 (MQ=2)
                                               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                      >  1:1358714/1‑101 (MQ=255)
                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.G.CG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                        >  2:1369533/1‑101 (MQ=2)
                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                     <  2:3711751/101‑1 (MQ=255)
                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                                    <  1:363515/101‑1 (MQ=255)
                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGCGGATCGAAACATCa                                                    >  2:1906603/1‑101 (MQ=255)
                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.AC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActaca                                                    <  1:7030/101‑5 (MQ=2)
                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.AC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActaca                                                    >  1:500958/1‑97 (MQ=2)
                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcg                                                     <  1:3387187/101‑4 (MQ=9)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.G.CG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                      <  2:444527/101‑2 (MQ=2)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.G.CG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                      <  2:96623/101‑2 (MQ=2)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.G.CG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                      <  2:3356722/101‑2 (MQ=2)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.G.CG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                      >  2:1426685/1‑100 (MQ=2)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGc                                                  >  1:3714607/1‑101 (MQ=255)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACATCGGc                                                  >  2:3566385/1‑101 (MQ=255)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.AC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                                  >  2:1309375/1‑95 (MQ=2)
                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.G.GG.CG.GCATGGATTCATCA.ACCGC.CAT..CGAGCGTCCCTCCCCCCGCGGGGGCGTGGGACGaaaaacca                                                    >  2:2610838/1‑96 (MQ=2)
                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GG.CG.GC.AGTG.TCAGGT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcca                                                  >  1:466959/1‑95 (MQ=2)
                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACA.CCAAT.GCCG.AA.GC.CAAG.GCCGCA.GCAGC.GGC..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAACGAAACaaaaa                                                   >  1:1778079/1‑97 (MQ=2)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                >  1:287457/1‑101 (MQ=255)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                >  1:1045238/1‑101 (MQ=255)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                 >  2:505930/1‑94 (MQ=9)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                 >  1:3920103/1‑94 (MQ=9)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                 >  1:3196182/1‑94 (MQ=9)
                                                     cccccccGCGGGGGCGTGGATCGAAACA..A.CT.G.AC.C...C.GGCA.ACGGCC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                                <  2:1102975/100‑9 (MQ=2)
                                                     cccccccGCGGGGGCGTGGATCGAAAC..GC.TG.G.CG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                 <  1:1246585/100‑8 (MQ=9)
                                                      ccccccGCGGGGGCGTGGATCGAAACT.GC.GT.G.CG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                                  >  1:1720171/1‑99 (MQ=2)
                                                      ccccccGCGGGGGCGTGGATCGAAAC..GC.TG.G.CG.CGCGC.CGCG.CCGGATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctc                                                <  1:1972157/101‑9 (MQ=9)
                                                       cccccGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgt                                              <  2:3391650/101‑1 (MQ=255)
                                                         cccGCGGGGGCGTGGATCGAAACT.GC.GT.G.CG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgcc                                               <  1:422083/101‑6 (MQ=0)
                                                         cccGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                                            <  1:1522105/101‑1 (MQ=255)
                                                         cccGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                                            >  1:3518724/1‑101 (MQ=255)
                                                           cgcgGGGGCGTGGATCGAAACT.GC.GT.G.CG.CG.CCATGAATTCATCG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccga                                             <  1:1934933/101‑8 (MQ=0)
                                                           cgcgGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                                          <  2:2427774/101‑1 (MQ=255)
                                                            gcgGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGTCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTg                                         <  2:206652/101‑1 (MQ=255)
                                                             cgGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                                        >  1:2586634/1‑101 (MQ=255)
                                                                gggCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCa                                     >  2:3068961/1‑101 (MQ=255)
                                                                 ggCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAg                                    <  1:1579571/101‑1 (MQ=255)
                                                                      ggATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa                               >  1:641595/1‑101 (MQ=255)
                                                                       gATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc                              <  2:3314361/101‑1 (MQ=255)
                                                                               cA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg                      <  1:3945236/101‑1 (MQ=255)
                                                                                      t.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt                 <  2:3257704/101‑1 (MQ=255)
                                                                                        g.cg.cG.GC.CGCA.GCGGCG.TCCGC.CTG..CGTG.GTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTc                <  1:3296079/101‑1 (MQ=255)
                                                                                                    gCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGGAACATAAGCTCGTCGTTGGTCAGCAGGACGATGTCCGTCGCTCCCCCcgcg        >  1:3555133/1‑101 (MQ=255)
                                                                                                     cA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCgg       >  1:139992/1‑101 (MQ=255)
                                                                                                          ggCT.TCCGC.GTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGc   <  2:952332/101‑1 (MQ=255)
                                                                                                           |                                                                                                         
CGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CG.CG.GC.CGCA.TCGGCT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCG  >  NC_014034/1396855‑1397054

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG