Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,955 C→A 12.6% G279G (GGC→ATA RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9550C→A12.6% 68.1 ‑0.3 ‑0.0283G279G (GGC→GGARCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (17/15):  ref base (109/113):  total (140/143)
Fisher's exact test strand distribution p-value = 7.09e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.95e-01

Only 100 of 308 total aligned reads displayed.

CGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1396855‑1397055
                                                                                                           |                                                                                                          
cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGTTCCTCCTCAGCCGTCGCTCCCCCCACGGAGGCGTCGACAGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGc                                                                                                            >  2:1391225/1‑101 (MQ=255)
cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGc                                                                                                            >  1:1340677/1‑101 (MQ=255)
 gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCt                                                                                                           >  2:3125390/1‑101 (MQ=255)
  tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGTATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCt.t                                                                                                         <  2:3478967/101‑1 (MQ=255)
  tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCt.t                                                                                                         >  2:639601/1‑101 (MQ=255)
     aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.Tc.cc                                                                                                     >  1:1108784/1‑100 (MQ=255)
       cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGc.c                                                                                                  <  2:4030411/101‑1 (MQ=255)
             cGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTg                                                                                          >  2:1126893/1‑101 (MQ=255)
                      gaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTccc                                                                                <  1:2725962/101‑1 (MQ=255)
                       aTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GGCGCTcccc                                                                               >  2:3204558/1‑101 (MQ=255)
                        tAGCCCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTccccc                                                                              <  2:824837/101‑1 (MQ=255)
                          gACCCCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCcg                                                                            <  1:756794/101‑1 (MQ=255)
                             cgcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCgg                                                                         <  2:701552/101‑1 (MQ=255)
                             cgcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCgg                                                                         <  1:1909163/101‑1 (MQ=255)
                                gcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCggggg                                                                      >  2:1222752/1‑101 (MQ=255)
                                 cgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                                     >  2:1772284/1‑101 (MQ=255)
                                  ggACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCg                                                                    <  1:1080464/101‑1 (MQ=255)
                                    aCCTCCTCCGCCGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTg                                                                  <  2:432769/101‑1 (MQ=255)
                                     ctcgcggttgcGTCGCTCCCCCCGCTGGGGCGTGGATCGAAACATGG.CTAT.GGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTg                                                                  <  2:1854079/91‑1 (MQ=0)
                                     cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                 >  1:2823536/1‑101 (MQ=255)
                                      tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                                                 <  1:58498/92‑1 (MQ=0)
                                      ggtcaacactgTCGCTCCACCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                 <  1:836338/91‑1 (MQ=9)
                                      attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.ACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                >  1:2052845/11‑101 (MQ=0)
                                      attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.ACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                <  1:290828/91‑1 (MQ=0)
                                       ttggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.ACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                               >  1:4226500/10‑101 (MQ=2)
                                       tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                               >  1:1018959/1‑101 (MQ=255)
                                        acccgcccGTCGCTCCCCCCGCGGGGGCGTAGATCGAAACT.GC.GT.G.CGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTgg                                                                 >  1:3769715/8‑101 (MQ=0)
                                         cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                              >  2:2627926/7‑101 (MQ=2)
                                          ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             <  1:521938/96‑1 (MQ=2)
                                          ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             >  2:412144/6‑101 (MQ=2)
                                          aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             <  2:3375370/95‑1 (MQ=9)
                                           cgcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.G.CGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                              <  2:764498/97‑1 (MQ=2)
                                           ccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.ACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           <  2:2243330/96‑1 (MQ=2)
                                           ccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.ACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           <  2:2439711/96‑1 (MQ=2)
                                           acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                            >  2:3598272/6‑101 (MQ=9)
                                              cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.ACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                        >  1:2148916/3‑100 (MQ=2)
                                              cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.ACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                        <  2:2016190/99‑2 (MQ=2)
                                              cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.ACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                        >  2:2687360/3‑100 (MQ=2)
                                              aCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                        >  2:3712400/1‑101 (MQ=255)
                                               tgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                        >  1:1676223/2‑100 (MQ=9)
                                               ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.ACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                                       >  2:2609413/2‑99 (MQ=2)
                                               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                       >  1:1358714/1‑101 (MQ=255)
                                               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                       >  1:3451065/1‑101 (MQ=255)
                                                gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                      <  1:3594269/101‑1 (MQ=255)
                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.ACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActaca                                                     <  1:2235987/101‑5 (MQ=2)
                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcg                                                      >  1:991203/1‑98 (MQ=9)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.G.CGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                                       <  2:96623/101‑2 (MQ=2)
                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.ACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacac                                                    >  1:3934293/1‑96 (MQ=2)
                                                  cGCCCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgc                                                     <  2:2850897/101‑5 (MQ=2)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GC.GT.G.CGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaaaccc                                                      >  2:3421640/1‑97 (MQ=2)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GGCG.GC.CGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCCGGGGCGTGGATccgaacccccc                                                    >  2:1566110/1‑91 (MQ=2)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                                    >  2:3732868/1‑96 (MQ=2)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACATGG.CTAT.GGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAcccgcc                                                    >  2:2928340/1‑96 (MQ=2)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACA..A.CT.G.ACC...C.GGCA.ACGGCC.TA.TGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                                   >  2:4172414/1‑95 (MQ=2)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                                    >  1:3905791/1‑96 (MQ=9)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                                    >  2:3038203/1‑96 (MQ=9)
                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACA.CCAAT.GCCGAA.GC.CAAG.GCCGCA.GC.AGC.GGC..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAACGAAACaaaaa                                                    >  1:1778079/1‑97 (MQ=2)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                 >  1:1045238/1‑101 (MQ=255)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                 >  1:1948322/1‑101 (MQ=255)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                 >  2:2547856/1‑101 (MQ=255)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                 >  1:287457/1‑101 (MQ=255)
                                                     cccccccGCGGGGGCGTGGATCGAAACA.GC.CT.G.CACG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                 <  1:2167685/100‑1 (MQ=255)
                                                     cccccccGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                  <  1:1246585/100‑8 (MQ=9)
                                                      nccncnGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctn                                                 <  1:894724/100‑9 (MQ=9)
                                                      ccccccGCGGGGGCGTGGATCGAAACT.GC.GT.G.CGCG.CCATGAATTCATCG.AG.CGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                                   >  1:1720171/1‑99 (MQ=2)
                                                      ccccccGCGGGGGCGTGGATCGAAACATGG.CTAT.GGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacg                                                 >  1:658081/1‑93 (MQ=0)
                                                       cccccGCGGGGGCGTGGATCGAAACATGG.CTAT.GGCG.GC.AGTG.TCAGGT.T..TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAcccgccacgt                                                >  2:1664950/1‑92 (MQ=2)
                                                       cccccGCGGGGGCGTGGATCGAAACA.CC.CG.G.CGCC.GC.AGCG.AGTGCG.TCTTGA.ACA..GGAT.GTCGCTCCCCCCGCAGGGGCGTGGATCGAAActatgctggc                                                >  1:3551874/1‑92 (MQ=2)
                                                       cccccGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcctcg                                                >  1:3950620/1‑92 (MQ=9)
                                                        ccccGCGGGGGCGTGGATCGAAAC..GC.TG.G.CGCGCGC.CGCG.CCGGATCTT.TGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGCGGATCGAAActcggcctcga                                               >  2:471760/1‑91 (MQ=2)
                                                         cccGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgtcg                                             <  1:1522105/101‑1 (MQ=255)
                                                           cgcgGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGCTCGAAACATCAGCTCGTCGtt                                           <  1:2400420/101‑1 (MQ=255)
                                                             cgGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                                         >  1:3257945/1‑101 (MQ=255)
                                                             cgGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTgg                                         >  1:2586634/1‑101 (MQ=255)
                                                              gggggCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                                        >  2:1269658/1‑101 (MQ=255)
                                                                 ggCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAg                                     <  1:1579571/101‑1 (MQ=255)
                                                                  gCGTGGATCGAACCA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTGGCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGc                                    <  1:4178471/101‑1 (MQ=255)
                                                                     tGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCgg                                 <  1:3361384/101‑1 (MQ=255)
                                                                       gATCGAAACC.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc                               <  1:4132035/101‑1 (MQ=255)
                                                                       gATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGAc                               <  2:3314361/101‑1 (MQ=255)
                                                                        aTCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg                              <  2:3891940/101‑1 (MQ=255)
                                                                        aTCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACg                              <  2:1077494/101‑1 (MQ=255)
                                                                          cGAAGCA.GC.CG.G.CGCG.GC.CGCA.TCTGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGAt                            <  2:2401862/101‑1 (MQ=255)
                                                                           gAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCTCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTCGTCAGCCGGACGATg                           >  1:476185/1‑101 (MQ=255)
                                                                               cA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTACg                       <  2:3518471/101‑1 (MQ=255)
                                                                                  gC.CT.G.CGCG.GC.CGCA.TCGCCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc                     <  2:1934989/101‑1 (MQ=255)
                                                                                     cT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGGTGTCCGTCGc                   >  1:3720226/1‑101 (MQ=255)
                                                                                     cT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGc                   >  2:2782294/1‑101 (MQ=255)
                                                                                      t.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt                  <  2:3257704/101‑1 (MQ=255)
                                                                                      t.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCt                  <  1:3125621/101‑1 (MQ=255)
                                                                                           gcg.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccc               <  2:1358614/101‑1 (MQ=255)
                                                                                           gcg.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccc               <  2:2162050/101‑1 (MQ=255)
                                                                                             g.gC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTccccc             >  2:1825463/1‑101 (MQ=255)
                                                                                                c.cGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCCGACGATGTCCGTCGCTccccccc           >  1:4079993/1‑100 (MQ=255)
                                                                                                    cA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCgg        >  1:139992/1‑101 (MQ=255)
                                                                                                     a.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCggg       >  2:1591196/1‑101 (MQ=255)
                                                                                                     a.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGCCAGCCGGACGATGTCCGTCCCTCCCCCCGCggg       >  1:3644290/1‑101 (MQ=255)
                                                                                                        cGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCCCCCCCCGCggggg     >  2:334141/1‑101 (MQ=255)
                                                                                                         ggCT.TC.CGC.GTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGc    <  2:952332/101‑1 (MQ=255)
                                                                                                           cT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCgg  >  2:544704/1‑100 (MQ=255)
                                                                                                           |                                                                                                          
CGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GC.CT.G.CGCG.GC.CGCA.TCGGCT.TC.CGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTCGCTCCCCCCGCGGGGGCGT  >  NC_014034/1396855‑1397055

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG