Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,165 C→G 5.5% R349R (CGC→CGG RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,1650C→G 5.5% 16.1 ‑1.1 ‑0.3180R349R (CGC→CGGRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/3):  ref base (88/82):  total (95/85)
Fisher's exact test strand distribution p-value = 3.38e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.12e-01

Only 100 of 201 total aligned reads displayed.

CTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA  >  NC_014034/1397065‑1397264
                                                                                                     |                                                                                                    
  gCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCg                                                                                                    <  1:1068490/101‑1 (MQ=255)
  gCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCg                                                                                                    >  1:1511323/1‑101 (MQ=255)
   ccGTCTTGAGTGATGGGGTGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGt                                                                                                   <  2:1814515/101‑1 (MQ=255)
    cGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTc                                                                                                  <  2:3083901/101‑1 (MQ=255)
    cGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTc                                                                                                  >  2:294507/1‑101 (MQ=255)
    cGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTc                                                                                                  >  2:352422/1‑101 (MQ=255)
     gTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCg                                                                                                 <  1:1772403/101‑1 (MQ=255)
     gTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCg                                                                                                 >  2:3890132/1‑101 (MQ=255)
        ttGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTc                                                                                              >  1:1742699/1‑101 (MQ=255)
        ttGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTc                                                                                              <  2:2823317/101‑1 (MQ=255)
         tGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.CGGCGCCGTCGCTcc                                                                                             >  1:3695848/1‑101 (MQ=255)
          gAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccc                                                                                            >  1:811832/1‑101 (MQ=255)
            gTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTccccc                                                                                          <  2:1419843/101‑1 (MQ=255)
             tGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccccc                                                                                         <  2:139968/101‑1 (MQ=255)
              gagggggCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcg                                                                                        <  2:2972493/98‑1 (MQ=255)
               gtGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTcccatagt                                                                                       <  2:525201/100‑6 (MQ=255)
                tGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCcgcg                                                                                      >  1:3148669/1‑101 (MQ=255)
                     cgcgAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACGTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGc                                                                                 <  1:2198165/101‑1 (MQ=255)
                         tgCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCCCCGCTCGCCCCCCATGTCTTCGACC.CTGCGCCGTCGCTCCCCCCCCGGGGCCCTgg                                                                             >  1:3420533/2‑101 (MQ=25)
                          gcgcaTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                            <  1:3483184/98‑1 (MQ=255)
                          gCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                            >  2:4141500/1‑101 (MQ=255)
                            acaTGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                          <  2:3604061/101‑1 (MQ=255)
                               tgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                       >  2:4102260/5‑101 (MQ=2)
                               tgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                       >  1:3903968/5‑101 (MQ=2)
                               tgcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                       >  1:3066766/5‑101 (MQ=2)
                               tGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                       >  2:2457535/1‑101 (MQ=255)
                                gcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                      <  1:1162229/98‑1 (MQ=2)
                                gcggtcgCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAAGCCGCCGACAAACG.CGGCAACCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                      >  1:2305169/4‑101 (MQ=2)
                                 tcgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTTGACC.GTGCGCCGACGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                     >  1:259411/1‑101 (MQ=255)
                                   gtcgCCCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgt                                                                   <  1:1991921/101‑1 (MQ=255)
                                     cgCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACgtgt                                                                 <  1:950253/101‑1 (MQ=255)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCt                                                               >  1:1315907/1‑101 (MQ=255)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCt                                                               <  1:2008743/101‑1 (MQ=255)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCt                                                               <  1:2969353/101‑1 (MQ=255)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTg                                                              >  1:1364363/1‑101 (MQ=255)
                                         ccccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCTGc                                                             >  2:110511/1‑101 (MQ=255)
                                          cccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGcc                                                            >  1:642185/1‑101 (MQ=255)
                                           ccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa                                                           <  2:1769045/101‑1 (MQ=255)
                                           ccccGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa                                                           <  1:2232481/101‑1 (MQ=255)
                                               gcgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cgac                                                      <  1:89785/101‑1 (MQ=255)
                                                cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cgacg                                                     >  1:3178997/1‑101 (MQ=255)
                                                cgGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCa.cgacg                                                     >  2:1606451/1‑101 (MQ=255)
                                                 gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGc                                                    >  1:568157/1‑101 (MQ=255)
                                                 gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGc                                                    >  2:509789/1‑101 (MQ=255)
                                                 gggggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGc                                                    >  2:3780070/1‑101 (MQ=255)
                                                 gggggCGTGGATCGAAACGTCCAACTCGCGTCACATGTCTTCGCCC.GCGCGCCGTCGCTCCCCCCGCGGGGACGTGGATCGAAACGTGTCTCTCA.CGATGc                                                    >  2:727303/1‑101 (MQ=25)
                                                  ggggGGTGGACGGAAAGGTCCGGCTCGCG.CACATTTCTTCGACC.GTGGCCCCTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGccc                                                  <  2:2349457/101‑1 (MQ=21)
                                                   gggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGccc                                                  <  1:3890391/101‑1 (MQ=255)
                                                   gggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGccc                                                  <  2:2319986/101‑1 (MQ=255)
                                                   gggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGccc                                                  >  1:1650930/1‑101 (MQ=255)
                                                   gggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGccc                                                  <  2:1747246/101‑1 (MQ=255)
                                                    ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCt                                                 <  2:3578564/101‑1 (MQ=255)
                                                    ggCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCt                                                 <  2:272666/101‑1 (MQ=255)
                                                      cGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGc                                               <  2:642108/101‑1 (MQ=255)
                                                       gTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGACA.CGAGGCCCTGCa                                              >  1:3654853/1‑101 (MQ=255)
                                                         ggATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGACACGTGTCTGCCA.CGACGCCCTGCAcc                                            >  2:586595/1‑101 (MQ=255)
                                                         ggATCGAAACGTCCAGCTCGCCCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCAcc                                            <  1:586659/101‑1 (MQ=255)
                                                           aTCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAg                                          <  2:3035281/101‑1 (MQ=255)
                                                             cGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGAt                                        >  1:3264612/1‑101 (MQ=255)
                                                              gAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATc                                       <  1:4208288/101‑1 (MQ=255)
                                                               aaaCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATcc                                      >  1:841627/1‑101 (MQ=255)
                                                               aaaCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATcc                                      <  2:43336/101‑1 (MQ=255)
                                                                aaCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCcg                                     <  2:1525554/101‑1 (MQ=255)
                                                                 aCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCcgc                                    <  1:1213022/101‑1 (MQ=255)
                                                                 aCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCcgc                                    >  1:3921396/1‑101 (MQ=255)
                                                                 aCGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CCACGCCCTGCACCAGATCcgc                                    <  2:259366/101‑1 (MQ=255)
                                                                  cGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCcgcg                                   >  1:2091556/1‑101 (MQ=255)
                                                                  cGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCcgcg                                   <  1:352473/101‑1 (MQ=255)
                                                                  cGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCcgcg                                   >  2:387832/1‑101 (MQ=255)
                                                                   gTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCgcgc                                  >  2:867784/1‑101 (MQ=255)
                                                                    tCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCGTCTGCCA.CGACGCCCTGCACCAGATCCGCGcc                                 >  1:3626133/1‑101 (MQ=255)
                                                                    gccAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGcc                                 <  2:1907883/100‑1 (MQ=255)
                                                                     ccAGCTCGCGCCACATGTCTTCGACC.GTTCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCg                                <  2:367840/101‑1 (MQ=255)
                                                                     ccAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCg                                >  2:3313588/1‑101 (MQ=255)
                                                                      cAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGt                               >  1:1607523/1‑101 (MQ=255)
                                                                       aGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTc                              <  1:3578384/101‑1 (MQ=255)
                                                                       aGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTc                              <  1:3357130/101‑1 (MQ=255)
                                                                         cTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGc                            <  1:2782513/101‑1 (MQ=255)
                                                                          tCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCt                           >  1:881533/1‑101 (MQ=255)
                                                                           cgcgCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTc                          <  1:297699/101‑1 (MQ=255)
                                                                             cgcCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTccc                        <  2:822904/101‑1 (MQ=255)
                                                                             cgcCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTccc                        >  2:3646698/1‑101 (MQ=255)
                                                                                cacaTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTcccccc                     >  2:645756/1‑101 (MQ=255)
                                                                                cacaTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTcccccc                     >  1:2768632/1‑101 (MQ=255)
                                                                                 acaTGTCCTCGTCC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCcg                    <  2:3719972/101‑1 (MQ=255)
                                                                                  caTGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCcgc                   >  1:3535047/1‑101 (MQ=255)
                                                                                     gTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggg                <  1:1474182/101‑1 (MQ=255)
                                                                                     gTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCggg                >  1:2912488/1‑101 (MQ=255)
                                                                                        ttCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGc             >  1:1392241/1‑101 (MQ=255)
                                                                                          cgccc.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGt           <  2:2207408/98‑1 (MQ=255)
                                                                                           gACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTg          <  2:3448630/101‑1 (MQ=255)
                                                                                              c.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt       >  1:1009997/1‑101 (MQ=255)
                                                                                                gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc      >  2:2547404/1‑101 (MQ=255)
                                                                                                  gcggCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCAACAAATATGTGGTCAACACTGTCGCTCCCCCCGCGGGGGCGTGGATCg     <  1:1891777/101‑1 (MQ=2)
                                                                                                  gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa    <  2:1359786/101‑1 (MQ=255)
                                                                                                   cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa   <  1:509846/101‑1 (MQ=255)
                                                                                                   cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa   <  2:1667430/101‑1 (MQ=255)
                                                                                                    gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa  >  2:487236/1‑101 (MQ=255)
                                                                                                    gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa  >  1:2140038/1‑101 (MQ=255)
                                                                                                    gcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa  <  2:2093395/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
CTGCCGTCTTGAGTGATGGGGCGCGAGCACATGTCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTCCAGCTCGCGCCACATGTCTTCGACC.GTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA  >  NC_014034/1397065‑1397264

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG