Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,497 C→T 10.7% T460I (ACC→ATC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,4970C→T10.7% 57.7 ‑0.6 0.0233T460I (ACC→ATC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (15/10):  ref base (107/101):  total (122/111)
Fisher's exact test strand distribution p-value = 5.26e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 266 total aligned reads displayed.

AACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA  >  NC_014034/1397397‑1397597
                                                                                                       |                                                                                                    
  ctctTCAATGGATATGGCAAAACCGTCCGCNACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCg                                                                                                    <  1:694252/101‑1 (MQ=255)
    ctgcAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCCCCGGt                                                                                                  <  1:1550163/98‑1 (MQ=255)
    ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGt                                                                                                  <  2:209077/101‑1 (MQ=255)
    ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGt                                                                                                  <  2:294673/101‑1 (MQ=255)
    ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGt                                                                                                  >  2:3891085/1‑101 (MQ=255)
     ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCT.CCATC.ACCCCGCAA.CCCCCCTCCCCCACCggcc                                                                                                 >  2:4074710/1‑99 (MQ=37)
     ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTc                                                                                                 <  2:1878763/101‑1 (MQ=255)
     ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTc                                                                                                 >  1:2361299/1‑101 (MQ=255)
        aaTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGcc                                                                                              >  2:1865724/1‑100 (MQ=255)
        aaTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCt                                                                                              >  1:3084181/1‑101 (MQ=255)
        aaTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCt                                                                                              >  2:4223808/1‑101 (MQ=255)
         aTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTc                                                                                             <  2:2675641/101‑1 (MQ=255)
         aTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTc                                                                                             >  1:1906737/1‑101 (MQ=255)
         aTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTc                                                                                             >  2:235653/1‑101 (MQ=255)
           ggATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTccc                                                                                           >  1:1205422/1‑101 (MQ=255)
             atatGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTccccc                                                                                         >  2:2571211/1‑101 (MQ=255)
                   cAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCggg                                                                                   >  1:54751/1‑101 (MQ=255)
                   cAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCggg                                                                                   >  1:2175900/1‑101 (MQ=255)
                     aaaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCggggg                                                                                 >  2:3866428/1‑101 (MQ=255)
                      aaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.GCCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGc                                                                                <  2:1677890/101‑1 (MQ=255)
                      aaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGc                                                                                <  1:487293/101‑1 (MQ=255)
                        ccGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGt                                                                              <  2:3817265/101‑1 (MQ=255)
                        ccGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGt                                                                              <  1:3269981/101‑1 (MQ=255)
                         cGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTg                                                                             >  2:2755617/1‑101 (MQ=255)
                          gTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTgg                                                                            >  2:795664/1‑101 (MQ=255)
                           tCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                           <  1:1251273/101‑1 (MQ=255)
                            ccGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                          >  1:2808785/1‑101 (MQ=255)
                              ataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGac                                                                          >  1:2099487/8‑100 (MQ=0)
                              ataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                          >  2:2191593/8‑101 (MQ=2)
                                 ccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                       <  2:2522027/97‑1 (MQ=2)
                                 aCATGTCGCTCCCCCCGCGGGGGCGAGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                     >  2:129997/1‑101 (MQ=255)
                                 aCATGTCGCGGCCCCCGCGGGCGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                     <  2:551662/101‑1 (MQ=255)
                                  cATGTCGCTCCCCTCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaac                                                                    <  1:3635841/101‑1 (MQ=255)
                                  cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaac                                                                    >  1:1381705/1‑101 (MQ=255)
                                  cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaac                                                                    <  1:1263074/101‑1 (MQ=255)
                                    tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaacaa                                                                  <  1:3906208/101‑1 (MQ=255)
                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAcc                                                                >  2:2887539/1‑101 (MQ=255)
                                       tgCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggt                                                                 <  1:3372010/100‑4 (MQ=2)
                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAaacggg                                                                 >  2:1539298/1‑98 (MQ=2)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCg                                                              >  1:1471036/1‑101 (MQ=255)
                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCg                                                              >  1:4236477/1‑101 (MQ=255)
                                          tCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAaacaaagatcg                                                            >  2:3747394/1‑95 (MQ=255)
                                          tCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGac                                                            >  2:2429433/1‑100 (MQ=255)
                                          tCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGAt                                                            <  1:2270457/101‑1 (MQ=255)
                                           ccccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATc                                                           <  2:1034924/101‑1 (MQ=255)
                                           ccccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATc                                                           >  1:3783229/1‑101 (MQ=255)
                                             ccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAaacggtgtccag                                                           >  1:856603/1‑92 (MQ=2)
                                             ccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCAt                                                         <  1:3327595/101‑1 (MQ=255)
                                              cccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATg                                                        >  1:4196833/1‑101 (MQ=255)
                                                cgcgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGc                                                      <  1:1073676/101‑1 (MQ=255)
                                                cgcgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCCCCCCCCGCGCAGGCGTGGCTCGAAACAACCCGATCATCGc                                                      >  1:1817886/1‑101 (MQ=38)
                                                  cgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                                                    >  1:2907940/1‑101 (MQ=255)
                                                   gggggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCg                                                   <  2:1205328/101‑1 (MQ=255)
                                                      ggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCt                                                >  1:1976380/1‑101 (MQ=255)
                                                      ggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCt                                                >  1:2060724/1‑101 (MQ=255)
                                                      ggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCt                                                >  1:2507805/1‑101 (MQ=255)
                                                      ggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCt                                                >  2:1822768/1‑101 (MQ=255)
                                                       gCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTc                                               <  1:3866687/101‑1 (MQ=255)
                                                        ggtcgATCGAAACCCCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCa                                              <  1:4159506/97‑1 (MQ=255)
                                                         gTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAg                                             <  2:1642257/101‑1 (MQ=255)
                                                           ggATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCTTCAgcg                                           >  1:1040197/1‑101 (MQ=255)
                                                            gATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcgc                                          >  2:3449092/1‑101 (MQ=255)
                                                             aTCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgcc                                         >  1:2675790/1‑101 (MQ=255)
                                                             aTCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgcc                                         <  2:3525675/101‑1 (MQ=255)
                                                             aTCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgcc                                         <  2:3228810/101‑1 (MQ=255)
                                                             aTCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGACGCTCCCCCCGCGGGGGCTTGGATCGAAACAACCCGATCAAGGCCCGCCTCAGcccc                                         >  1:981536/1‑101 (MQ=38)
                                                               cGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgccgc                                       >  1:3492469/1‑101 (MQ=255)
                                                                 aaaCACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCgcgc                                     >  1:1274786/1‑101 (MQ=255)
                                                                  aaCACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCt                                    >  2:2104698/1‑101 (MQ=255)
                                                                   acgccG.TCATC.ACCACGCGA.CCGCCCTGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGCATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTg                                   <  2:691288/98‑1 (MQ=38)
                                                                   acacCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCgcgccg                                   <  2:2060586/101‑3 (MQ=255)
                                                                          tcatc.aCCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCg                             >  1:2413300/1‑101 (MQ=255)
                                                                          tcatc.aCCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCg                             >  1:1848153/1‑101 (MQ=255)
                                                                           catc.aCCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGc                            >  2:1131811/1‑101 (MQ=255)
                                                                           catc.aCCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGc                            >  2:755892/1‑101 (MQ=255)
                                                                            atc.aCCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCt                           >  1:2630008/1‑101 (MQ=255)
                                                                              c.accacGCAC.CCGCCCTGCCCCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcc                         <  1:1604357/101‑1 (MQ=255)
                                                                              c.accacGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcc                         >  1:3683751/1‑101 (MQ=255)
                                                                                accacGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTccc                        <  2:3492217/101‑1 (MQ=255)
                                                                                   ccGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcccccc                     <  1:795742/100‑1 (MQ=255)
                                                                                   acGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcccccc                     <  2:2629816/101‑1 (MQ=255)
                                                                                    cGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcg                    >  1:615269/1‑101 (MQ=255)
                                                                                     gCAA.CCGCCCTGCCCCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgc                   <  2:1249491/101‑1 (MQ=255)
                                                                                        a.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCggg                >  1:932946/1‑101 (MQ=255)
                                                                                          ccGCCCTGCACCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCgggg               <  1:2755836/101‑1 (MQ=255)
                                                                                            gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGc             <  2:931474/101‑1 (MQ=255)
                                                                                             cccTGAACCTCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCggggggc            <  1:4043334/101‑3 (MQ=255)
                                                                                              ccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGt           <  1:3769773/101‑1 (MQ=255)
                                                                                               cTGCACCACCGGACGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTAACGTCGCTCCCCCCGCGGGGGCGTg          <  1:1666461/101‑1 (MQ=255)
                                                                                                tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTgg         >  2:315244/1‑101 (MQ=255)
                                                                                                 gCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAGCAACCCGATCGTGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGa        <  2:2381406/101‑1 (MQ=255)
                                                                                                 gCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGa        <  1:2713397/101‑1 (MQ=255)
                                                                                                  ccccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGAt       <  1:2577986/99‑1 (MQ=255)
                                                                                                  caccccCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGAt       <  2:3343188/101‑1 (MQ=255)
                                                                                                  caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGAt       <  2:3126426/101‑1 (MQ=255)
                                                                                                  caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGAt       >  2:3070015/1‑101 (MQ=255)
                                                                                                  caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGAt       >  1:3549829/1‑101 (MQ=255)
                                                                                                  caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGAt       <  1:3449344/101‑1 (MQ=255)
                                                                                                   accaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATc      <  2:1378326/101‑1 (MQ=255)
                                                                                                      accGGTCGCTACCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa   >  2:4520/1‑101 (MQ=255)
                                                                                                       |                                                                                                    
AACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAA  >  NC_014034/1397397‑1397597

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG