Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,547 C→G 7.1% R477G (CGC→GGG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5470C→G 7.1% 40.7 ‑0.2 ‑0.1296R477G (CGC→GGC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (11/10):  ref base (132/143):  total (143/153)
Fisher's exact test strand distribution p-value = 8.22e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.79e-01

Only 100 of 311 total aligned reads displayed.

CGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCG  >  NC_014034/1397447‑1397647
                                                                                                       |                                                                                                       
 gggggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..Cg                                                                                                        <  2:1205328/101‑1 (MQ=255)
 gggggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..Cg                                                                                                        <  1:3783969/101‑1 (MQ=255)
    ggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCt                                                                                                     >  1:2507805/1‑101 (MQ=255)
    ggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCt                                                                                                     >  1:2060724/1‑101 (MQ=255)
     gCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTc                                                                                                    <  1:3367452/101‑1 (MQ=255)
     gCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTc                                                                                                    <  1:3866687/101‑1 (MQ=255)
     gCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTc                                                                                                    <  1:3287726/101‑1 (MQ=255)
      cGTGGATCTACACGCCGTCATCCCCACGCAACCGCCCTGCACCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.a                                                                                                  <  2:3528384/101‑1 (MQ=37)
       gTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.Ag                                                                                                 >  1:931559/1‑101 (MQ=255)
       gTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.Ag                                                                                                 >  2:2628134/1‑101 (MQ=255)
        tGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.Agc                                                                                                <  1:163463/101‑1 (MQ=255)
         ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.Agcg                                                                                               >  2:410366/1‑101 (MQ=255)
           aTCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGACGCTCCCCCCGCGGGGGCTTGGATCGAAACAACCCGATCAAGGC.C..CGCCTC.AGcccc                                                                                             >  1:981536/1‑101 (MQ=38)
             cGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGcgccgc                                                                                           >  1:3492469/1‑101 (MQ=255)
              gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCcgcg                                                                                          >  2:2488461/1‑101 (MQ=255)
               aaaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCgcgc                                                                                         >  1:1274786/1‑101 (MQ=255)
                aaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCCCGCt                                                                                        >  1:1936403/1‑101 (MQ=255)
                       tcatcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCg                                                                               >  1:1802766/1‑101 (MQ=255)
                        catcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGc                                                                              >  2:1131811/1‑101 (MQ=255)
                         atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCt                                                                             >  1:2630008/1‑101 (MQ=255)
                           caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTcc                                                                           >  1:3683751/1‑101 (MQ=255)
                            accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTccc                                                                          <  2:3492217/101‑1 (MQ=255)
                               ccGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTcccccc                                                                       <  1:795742/100‑1 (MQ=255)
                               acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCATGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTcccccc                                                                       >  2:2159784/1‑101 (MQ=255)
                                cGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcg                                                                      <  2:3666935/101‑1 (MQ=255)
                                 gCAACCGCCCTGCCCCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                     <  2:1249491/101‑1 (MQ=255)
                                 gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                     >  1:651409/1‑101 (MQ=255)
                                 gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                     >  1:1251627/1‑101 (MQ=255)
                                  cAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcgcg                                                                    >  2:72946/1‑101 (MQ=255)
                                   aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgg                                                                   >  1:2678478/1‑101 (MQ=255)
                                     ccGCCCTGCACCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgggg                                                                 <  1:2755836/101‑1 (MQ=255)
                                      cGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCggggg                                                                <  2:1906617/101‑1 (MQ=255)
                                       gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                               <  2:3083951/101‑1 (MQ=255)
                                       gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                               <  2:931474/101‑1 (MQ=255)
                                       cgccccgcccatcGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                               <  2:1848033/93‑1 (MQ=255)
                                        cccTGAACCTCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCggggggc                                                              <  1:4043334/101‑3 (MQ=255)
                                          cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                            >  1:2704066/1‑101 (MQ=255)
                                           tGCACCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                           <  1:2986720/101‑1 (MQ=255)
                                           tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                           <  1:1546347/101‑1 (MQ=255)
                                           ggaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                          <  1:3924726/91‑1 (MQ=2)
                                            gCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAGCAACCCGATCGTGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                          <  2:2381406/101‑1 (MQ=255)
                                            gCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                          >  1:1201113/1‑101 (MQ=255)
                                             ccccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         <  2:387181/99‑1 (MQ=255)
                                             caccccCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         <  2:3343188/101‑1 (MQ=255)
                                             caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         <  1:3449344/101‑1 (MQ=255)
                                             caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                         <  2:2886389/101‑1 (MQ=255)
                                              cgcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAAT.CG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                        <  1:2405255/95‑1 (MQ=0)
                                               ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                       <  2:2089056/101‑1 (MQ=255)
                                                 gagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                      >  1:4134223/5‑101 (MQ=2)
                                                 gagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                      >  1:1988767/5‑101 (MQ=2)
                                                 ctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     >  2:1804106/5‑101 (MQ=2)
                                                 accGGTCGCTACCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                     >  2:4520/1‑101 (MQ=255)
                                                  ccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                    >  2:789212/1‑101 (MQ=255)
                                                   cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                   <  1:878050/101‑1 (MQ=255)
                                                    cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                   >  2:245646/2‑101 (MQ=2)
                                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                 >  1:101328/1‑101 (MQ=2)
                                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                 >  1:1460937/1‑101 (MQ=255)
                                                     gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                 <  2:54736/101‑1 (MQ=255)
                                                      tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGGGGGGATCGaaaagggg                                               >  2:515388/1‑96 (MQ=2)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGGGGATCGCAACaaa                                                >  1:2883715/1‑99 (MQ=0)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                               <  2:2243825/101‑1 (MQ=255)
                                                       cGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                               >  1:2120637/1‑101 (MQ=255)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                             <  2:643889/101‑7 (MQ=2)
                                                        gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                              <  2:1381605/101‑1 (MQ=255)
                                                         cTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                              <  2:1720056/101‑5 (MQ=2)
                                                          tCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgcg                                            >  2:3228697/1‑101 (MQ=255)
                                                            cccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                          <  2:3743034/101‑7 (MQ=2)
                                                             ccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                         <  2:2120492/101‑1 (MQ=255)
                                                             ccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                         <  2:1426057/101‑1 (MQ=255)
                                                              cccGCGGGGGCCTGGATCGAAACACCCCGAACCT.GC.C..CGCGGCGAACACCGATAC.CG.CGTCGCGCCCCCCGCGGGGGCGTGGAGCGGCACATcgatgctaa                                        >  2:2843749/1‑93 (MQ=2)
                                                                   gggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCgg                                   >  1:2183689/1‑100 (MQ=255)
                                                                   gggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                   >  2:1093779/1‑101 (MQ=255)
                                                                   gggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGACAGGCGt                                   >  2:4226721/1‑101 (MQ=255)
                                                                    ggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                  >  1:104270/1‑101 (MQ=255)
                                                                    ggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                  <  2:3004201/101‑1 (MQ=255)
                                                                     gggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                                 <  2:204088/101‑1 (MQ=255)
                                                                     gggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                                 <  1:4224070/101‑1 (MQ=255)
                                                                      ggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                                >  1:1912902/1‑101 (MQ=255)
                                                                        cGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                              <  1:1677677/101‑1 (MQ=255)
                                                                        cGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                              <  1:1398921/101‑1 (MQ=255)
                                                                         gTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                             <  2:3142684/101‑1 (MQ=255)
                                                                          tGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                            >  2:2905887/1‑101 (MQ=255)
                                                                          tGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                            >  2:348309/1‑101 (MQ=255)
                                                                          tGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCCTGAcccgc                            >  1:3966656/1‑101 (MQ=255)
                                                                            gATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                          >  1:3570482/1‑101 (MQ=255)
                                                                                gAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                      >  1:3105978/1‑101 (MQ=255)
                                                                                   acaacCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt                   <  2:3782972/101‑1 (MQ=255)
                                                                                   acaacCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt                   >  1:556832/1‑101 (MQ=255)
                                                                                    caacCCTATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc                  <  2:2353533/101‑1 (MQ=255)
                                                                                    caacCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc                  >  1:1484331/1‑101 (MQ=255)
                                                                                     aacCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                 >  1:2311402/1‑101 (MQ=255)
                                                                                         cGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCg             >  1:3219472/1‑101 (MQ=255)
                                                                                            tCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTc          <  2:905627/101‑1 (MQ=255)
                                                                                            tCATGCC.C..CGCCGC.AGCGCCGCGCT.GA.CCTNGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTc          <  1:185883/101‑1 (MQ=255)
                                                                                             cATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGCGGCCCGCCCCGCCCATCCGGCGCCcc         >  2:2757778/1‑101 (MQ=37)
                                                                                              aTGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc        >  1:740073/1‑101 (MQ=255)
                                                                                               tGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccc       <  2:1997584/101‑1 (MQ=255)
                                                                                                ggC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccccc      >  2:1602769/1‑101 (MQ=255)
                                                                                                ggC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCCCCCCCCGGGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccccc      <  2:2691099/101‑1 (MQ=255)
                                                                                                 gC.C..CGCCTC.GGCGCCGCGCT.CA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTcccccc     <  1:3891344/101‑1 (MQ=255)
                                                                                                       |                                                                                                       
CGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCG  >  NC_014034/1397447‑1397647

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG