Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,549 C→G 9.0% R477R (CGC→GGG RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5490C→G 9.0% 51.4 ‑0.9 ‑0.0289R477R (CGC→CGGRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (10/15):  ref base (126/123):  total (139/150)
Fisher's exact test strand distribution p-value = 4.02e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.82e-01

Only 100 of 304 total aligned reads displayed.

GGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGG  >  NC_014034/1397449‑1397649
                                                                                                       |                                                                                                       
  ggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCt                                                                                                       >  1:2060724/1‑101 (MQ=255)
   gCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTc                                                                                                      <  1:3866687/101‑1 (MQ=255)
   gCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTc                                                                                                      <  1:3367452/101‑1 (MQ=255)
   gCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTc                                                                                                      <  1:3287726/101‑1 (MQ=255)
    cGTGGATCTACACGCCGTCATCCCCACGCAACCGCCCTGCACCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.a                                                                                                    <  2:3528384/101‑1 (MQ=37)
    cGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.a                                                                                                    <  1:2654887/101‑1 (MQ=255)
    cGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.a                                                                                                    <  1:2429609/101‑1 (MQ=255)
     gTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.Ag                                                                                                   <  2:1642257/101‑1 (MQ=255)
     gTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.Ag                                                                                                   >  1:931559/1‑101 (MQ=255)
     gTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.Ag                                                                                                   >  2:2628134/1‑101 (MQ=255)
         aTCGAAACACCGTCATCCCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGcgcc                                                                                               <  2:1234211/101‑1 (MQ=255)
         aTCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGcgcc                                                                                               <  2:3228810/101‑1 (MQ=255)
           cGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGcgccgc                                                                                             >  1:3492469/1‑101 (MQ=255)
            gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCcgcg                                                                                            >  2:1097438/1‑101 (MQ=255)
            gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCcgcg                                                                                            >  2:2488461/1‑101 (MQ=255)
            gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCcgcg                                                                                            >  1:1474346/1‑101 (MQ=255)
              aaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCCCGCt                                                                                          >  1:1936403/1‑101 (MQ=255)
               acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCgcgccg                                                                                         <  2:2060586/101‑3 (MQ=255)
                   cGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGt                                                                                   >  2:185845/1‑101 (MQ=255)
                     tcatcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCg                                                                                 >  1:1802766/1‑101 (MQ=255)
                       atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCt                                                                               >  1:2381582/1‑101 (MQ=255)
                         caccacGCACCCGCCCTGCCCCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTcc                                                                             <  1:1604357/101‑1 (MQ=255)
                         caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTcc                                                                             >  1:3683751/1‑101 (MQ=255)
                          accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTccc                                                                            <  2:3492217/101‑1 (MQ=255)
                            cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTccccc                                                                          <  1:3851955/101‑1 (MQ=255)
                            cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTccccc                                                                          >  1:3004426/1‑101 (MQ=255)
                             ccGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTcccccc                                                                         <  1:795742/100‑1 (MQ=255)
                             acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCATGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTcccccc                                                                         >  2:2159784/1‑101 (MQ=255)
                              cgcgcccGCCCTGCACCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcg                                                                        <  2:1353752/96‑1 (MQ=255)
                              cGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcg                                                                        <  2:3666935/101‑1 (MQ=255)
                               gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                       >  1:651409/1‑101 (MQ=255)
                               gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                       >  1:1251627/1‑101 (MQ=255)
                                cAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcgcg                                                                      >  2:72946/1‑101 (MQ=255)
                                 aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgg                                                                     >  2:2926004/1‑101 (MQ=255)
                                   ccGCCCTGCACCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgggg                                                                   <  1:2755836/101‑1 (MQ=255)
                                     gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                 <  2:931474/101‑1 (MQ=255)
                                      cccTGAACCTCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCggggggc                                                                <  1:4043334/101‑3 (MQ=255)
                                         tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                             >  2:315244/1‑101 (MQ=255)
                                         ggaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                            <  1:3924726/91‑1 (MQ=2)
                                          gCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAGCAACCCGATCGTGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                            <  2:2381406/101‑1 (MQ=255)
                                          gCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                            >  1:1201113/1‑101 (MQ=255)
                                           ccccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                           <  1:2577986/99‑1 (MQ=255)
                                           caccccCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                           <  2:3343188/101‑1 (MQ=255)
                                           caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                           <  1:3449344/101‑1 (MQ=255)
                                           caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                           >  2:3070015/1‑101 (MQ=255)
                                           caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                           <  2:3126426/101‑1 (MQ=255)
                                            accaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                          >  2:1157820/1‑101 (MQ=255)
                                             tggagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATc                                                          <  1:983391/95‑1 (MQ=0)
                                             ccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                         <  2:2089056/101‑1 (MQ=255)
                                               gagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                        >  1:4134223/5‑101 (MQ=2)
                                               gagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                        >  1:1988767/5‑101 (MQ=2)
                                               acgggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCCCCCCCCGCGGGGGCGTGGATCGaa                                                       <  1:130021/98‑1 (MQ=255)
                                               accGGTCGCTACCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                       >  2:4520/1‑101 (MQ=255)
                                                ccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                      >  2:789212/1‑101 (MQ=255)
                                                 cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                     <  1:878050/101‑1 (MQ=255)
                                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAAT.CG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                    >  1:1497694/1‑100 (MQ=2)
                                                  cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                     >  2:245646/2‑101 (MQ=2)
                                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAAT.CG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcct                                                   >  2:3900579/1‑99 (MQ=2)
                                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                   >  1:101328/1‑101 (MQ=2)
                                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                   <  2:54736/101‑1 (MQ=255)
                                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGGGGGGATCGaaaagggg                                                 >  2:515388/1‑96 (MQ=2)
                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGGGGATCGCAACaaa                                                  >  1:2883715/1‑99 (MQ=0)
                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                                 <  2:1936277/101‑1 (MQ=255)
                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                                 <  2:2243825/101‑1 (MQ=255)
                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                                 >  1:2120637/1‑101 (MQ=255)
                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                               <  2:643889/101‑7 (MQ=2)
                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgat                                               <  2:1734567/101‑4 (MQ=2)
                                                        cccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                              <  2:1819103/100‑5 (MQ=2)
                                                         ccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatga                                             >  2:4146519/1‑96 (MQ=2)
                                                         ccccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCgg                                             >  2:1398822/1‑101 (MQ=255)
                                                          cccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                            <  2:3743034/101‑7 (MQ=2)
                                                           ccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                           <  2:2120492/101‑1 (MQ=255)
                                                            cccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGGAAAATCCGCGGATc                                          >  1:2691305/1‑101 (MQ=255)
                                                            cccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                          <  2:2754870/101‑1 (MQ=255)
                                                            cccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                          <  2:2891594/101‑1 (MQ=255)
                                                            cccGCGGGGGCCTGGATCGAAACACCCCGAACCT.GC.C..CGCGGCGAACACCGATAC.CG.CGTCGCGCCCCCCGCGGGGGCGTGGAGCGGCACATcgatgctaa                                          >  2:2843749/1‑93 (MQ=2)
                                                                cgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                      >  1:1426203/1‑101 (MQ=255)
                                                                 gggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCgg                                     >  1:2183689/1‑100 (MQ=255)
                                                                 gggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGACAGGCGt                                     >  2:4226721/1‑101 (MQ=255)
                                                                  ggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                    >  1:104270/1‑101 (MQ=255)
                                                                   gggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                                   <  1:4224070/101‑1 (MQ=255)
                                                                    ggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                                  >  1:1634701/1‑101 (MQ=255)
                                                                    ggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                                  >  1:1912902/1‑101 (MQ=255)
                                                                      cGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                <  1:1677677/101‑1 (MQ=255)
                                                                       gTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                               <  2:2678272/101‑1 (MQ=255)
                                                                        tGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                              >  2:348309/1‑101 (MQ=255)
                                                                        tGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                              >  2:203229/1‑101 (MQ=255)
                                                                        tGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCCTGAcccgc                              >  1:3966656/1‑101 (MQ=255)
                                                                          gATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                            >  1:3570482/1‑101 (MQ=255)
                                                                            tCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                          >  1:2253154/1‑101 (MQ=255)
                                                                              gAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                        >  1:3105978/1‑101 (MQ=255)
                                                                                accaacCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCa                      <  2:1474244/99‑1 (MQ=255)
                                                                                 acaacCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt                     <  2:3782972/101‑1 (MQ=255)
                                                                                 acaacCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt                     >  1:556832/1‑101 (MQ=255)
                                                                                  caacCCTATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc                    <  2:2353533/101‑1 (MQ=255)
                                                                                       cGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCg               >  1:3219472/1‑101 (MQ=255)
                                                                                          tCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTc            <  2:905627/101‑1 (MQ=255)
                                                                                          tCATGCC.C..CGCCGC.AGCGCCGCGCT.GA.CCTNGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTc            <  1:185883/101‑1 (MQ=255)
                                                                                            aTGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc          >  1:740073/1‑101 (MQ=255)
                                                                                               gC.C..CGCCTC.GGCGCCGCGCT.CA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTcccccc       <  1:3891344/101‑1 (MQ=255)
                                                                                                       |                                                                                                       
GGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGG  >  NC_014034/1397449‑1397649

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG