Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,552 C→G 9.8% L478L (CTC→GTG RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5520C→G 9.8% 52.9 ‑1.2 ‑0.0283L478L (CTC→CTGRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (10/15):  ref base (122/115):  total (138/145)
Fisher's exact test strand distribution p-value = 3.00e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.66e-01

Only 100 of 301 total aligned reads displayed.

GCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGG  >  NC_014034/1397452‑1397650
                                                                                                       |                                                                                                     
gCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTc                                                                                                       <  1:3866687/101‑1 (MQ=255)
 cGTGGATCTACACGCCGTCATCCCCACGCAACCGCCCTGCACCGCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.a                                                                                                     <  2:3528384/101‑1 (MQ=37)
 cGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.a                                                                                                     <  1:2654887/101‑1 (MQ=255)
  gTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.Ag                                                                                                    >  1:931559/1‑101 (MQ=255)
      aTCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGcgcc                                                                                                <  2:3228810/101‑1 (MQ=255)
      aTCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGcgcc                                                                                                <  2:210081/101‑1 (MQ=255)
      aTCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGACGCTCCCCCCGCGGGGGCTTGGATCGAAACAACCCGATCAAGGC.C..CGCCTC.AGcccc                                                                                                >  1:981536/1‑101 (MQ=38)
        cGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGcgccgc                                                                                              >  1:3492469/1‑101 (MQ=255)
         gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCcgcg                                                                                             >  1:1474346/1‑101 (MQ=255)
           aaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCCCGCt                                                                                           >  1:1936403/1‑101 (MQ=255)
                  tcatcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCg                                                                                  >  1:1802766/1‑101 (MQ=255)
                  tcatcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCg                                                                                  >  1:2413300/1‑101 (MQ=255)
                   catcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGc                                                                                 >  2:1131811/1‑101 (MQ=255)
                    atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGcc                                                                                >  1:2243984/1‑100 (MQ=255)
                      caccacGCACCCGCCCTGCCCCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTcc                                                                              <  1:1604357/101‑1 (MQ=255)
                      caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTcc                                                                              >  1:3683751/1‑101 (MQ=255)
                       accacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTccc                                                                             <  2:3492217/101‑1 (MQ=255)
                         cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTccccc                                                                           <  1:3851955/101‑1 (MQ=255)
                          ccGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTcccccc                                                                          <  1:795742/100‑1 (MQ=255)
                          acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTcccccc                                                                          <  2:2629816/101‑1 (MQ=255)
                           cGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcg                                                                         <  2:3666935/101‑1 (MQ=255)
                            gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                        >  1:1251627/1‑101 (MQ=255)
                            gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                        >  1:651409/1‑101 (MQ=255)
                             cAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCcgcg                                                                       >  2:72946/1‑101 (MQ=255)
                              aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgg                                                                      >  2:2926004/1‑101 (MQ=255)
                              aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgg                                                                      <  1:2104836/101‑1 (MQ=255)
                                  gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                  <  2:931474/101‑1 (MQ=255)
                                   cccTGAACCTCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCggggggc                                                                 <  1:4043334/101‑3 (MQ=255)
                                     cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                               <  2:2413124/101‑1 (MQ=255)
                                      tGCACCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                              <  1:2986720/101‑1 (MQ=255)
                                      tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                              <  2:1256787/101‑1 (MQ=255)
                                      tGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                              >  2:315244/1‑101 (MQ=255)
                                      ggaacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                             <  1:3924726/91‑1 (MQ=2)
                                       gCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                             >  1:1201113/1‑101 (MQ=255)
                                       gCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                             <  1:2713397/101‑1 (MQ=255)
                                        ccccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                            <  1:2577986/99‑1 (MQ=255)
                                        caccccCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                            <  2:3343188/101‑1 (MQ=255)
                                        caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                            <  2:3126426/101‑1 (MQ=255)
                                        caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                            >  2:3070015/1‑101 (MQ=255)
                                        caccacCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                            <  1:3449344/101‑1 (MQ=255)
                                          cgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          <  2:2039215/95‑1 (MQ=2)
                                            gagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                         >  1:4134223/5‑101 (MQ=2)
                                            gagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                         <  2:2499170/97‑1 (MQ=2)
                                            acgggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCCCCCCCCGCGGGGGCGTGGATCGaa                                                        <  1:130021/98‑1 (MQ=255)
                                            accGGTCGCTACCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                        >  2:4520/1‑101 (MQ=255)
                                             ccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                       >  2:789212/1‑101 (MQ=255)
                                             agcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                        >  2:187783/4‑101 (MQ=2)
                                              cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                      <  1:878050/101‑1 (MQ=255)
                                               ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAAT.CG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                     >  1:1497694/1‑100 (MQ=2)
                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAAT.CG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcct                                                    >  2:3900579/1‑99 (MQ=2)
                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                    >  1:101328/1‑101 (MQ=2)
                                                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                    <  2:54736/101‑1 (MQ=255)
                                                 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGGGGGGATCGaaaagggg                                                  >  2:515388/1‑96 (MQ=2)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTT.CACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                                <  2:643889/101‑7 (MQ=2)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                 <  2:1381605/101‑1 (MQ=255)
                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                 >  1:3280168/1‑101 (MQ=255)
                                                    cTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                                 <  2:1213464/101‑5 (MQ=2)
                                                     tCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                                >  1:3198954/1‑96 (MQ=0)
                                                     cccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                               <  2:1819103/100‑5 (MQ=2)
                                                      ccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatga                                              >  1:2880808/1‑96 (MQ=2)
                                                      ccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatga                                              >  2:4146519/1‑96 (MQ=2)
                                                      ccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGC.AGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                               <  1:3341469/101‑7 (MQ=0)
                                                       cccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.G.AACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                             <  2:3743034/101‑7 (MQ=2)
                                                        ccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAt                                            <  2:1426057/101‑1 (MQ=255)
                                                         cccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGGAAAATCCGCGGATc                                           >  1:2691305/1‑101 (MQ=255)
                                                         cccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                           <  2:2891594/101‑1 (MQ=255)
                                                         cccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                           <  2:2127763/101‑1 (MQ=255)
                                                         cccGCGGGGGCCTGGATCGAAACACCCCGAACCT.GC.C..CGCGGCGAACACCGATAC.CG.CGTCGCGCCCCCCGCGGGGGCGTGGAGCGGCACATcgatgctaa                                           >  2:2843749/1‑93 (MQ=2)
                                                             cgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                       >  1:1426203/1‑101 (MQ=255)
                                                             cgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                       >  1:1997710/1‑101 (MQ=255)
                                                              gggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCgg                                      >  2:1651620/1‑100 (MQ=255)
                                                              gggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                      >  2:1093779/1‑101 (MQ=255)
                                                              gggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGACAGGCGt                                      >  2:4226721/1‑101 (MQ=255)
                                                               ggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                     >  1:104270/1‑101 (MQ=255)
                                                                gggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                                    <  1:4224070/101‑1 (MQ=255)
                                                                 ggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                                   <  2:1976254/101‑1 (MQ=255)
                                                                 ggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                                   >  1:1912902/1‑101 (MQ=255)
                                                                 ggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                                   >  1:1634701/1‑101 (MQ=255)
                                                                   cGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                 <  1:1677677/101‑1 (MQ=255)
                                                                     tGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                               >  2:348309/1‑101 (MQ=255)
                                                                     tGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                               >  2:2247673/1‑101 (MQ=255)
                                                                     tGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCCTGAcccgc                               >  1:3966656/1‑101 (MQ=255)
                                                                       gATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                             >  1:3570482/1‑101 (MQ=255)
                                                                              acaacCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt                      <  2:2183544/101‑1 (MQ=255)
                                                                              acaacCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt                      >  1:556832/1‑101 (MQ=255)
                                                                              acaacCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCAt                      <  2:3782972/101‑1 (MQ=255)
                                                                               caacCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc                     <  2:2470071/101‑1 (MQ=255)
                                                                                aacCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                    >  1:2311402/1‑101 (MQ=255)
                                                                                aacCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                    >  2:2073882/1‑101 (MQ=255)
                                                                                     gATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGc               >  2:1528414/1‑101 (MQ=255)
                                                                                      aTCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt              <  2:2361123/101‑1 (MQ=255)
                                                                                       tCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTc             <  2:905627/101‑1 (MQ=255)
                                                                                       tCATGCC.C..CGCCGC.AGCGCCGCGCT.GA.CCTNGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTc             <  1:185883/101‑1 (MQ=255)
                                                                                        cATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGCGGCCCGCCCCGCCCATCCGGCGCCcc            >  2:2757778/1‑101 (MQ=37)
                                                                                         aTGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc           >  1:740073/1‑101 (MQ=255)
                                                                                           ggC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCCCCCCCCGGGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccccc         <  2:2691099/101‑1 (MQ=255)
                                                                                            gC.C..CGCCTC.GGCGCCGCGCT.CA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTcccccc        <  1:3891344/101‑1 (MQ=255)
                                                                                                  cGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcgcg     >  1:3095136/1‑101 (MQ=255)
                                                                                                     cTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgggg  >  2:174729/1‑101 (MQ=255)
                                                                                                     cTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgggg  >  1:2159585/1‑101 (MQ=255)
                                                                                                       |                                                                                                     
GCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTC.AGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGG  >  NC_014034/1397452‑1397650

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG