Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,871 G→A 2.0% intergenic (+175/+1723) RCAP_rcc01307 → / ← RCAP_rcc01309 hypothetical protein/IS66 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,8710G→A 2.0% 3.1 ‑1.0 ‑0.7200intergenic (+175/+1723)RCAP_rcc01307/RCAP_rcc01309hypothetical protein/IS66 family transposase
Reads supporting (aligned to +/- strand):  new base (1/3):  ref base (99/96):  total (100/100)
Fisher's exact test strand distribution p-value = 3.69e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.88e-01

Only 100 of 214 total aligned reads displayed.

TCCCCCCGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAA  >  NC_014034/1397771‑1397970
                                                                                                     |                                                                                                   
  cccccGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCa                                                                                                   >  1:2830496/1‑101 (MQ=255)
      cgcgGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTc                                                                                               <  2:750811/101‑1 (MQ=255)
       gcgGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCa                                                                                              >  2:1916056/1‑101 (MQ=255)
       gcgGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCa                                                                                              <  1:2784579/101‑1 (MQ=255)
       gcgGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCa                                                                                              <  1:1479359/101‑1 (MQ=255)
       gcgGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCa                                                                                              <  2:3528254/101‑1 (MQ=255)
        cgGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAg                                                                                             >  2:2641209/1‑101 (MQ=255)
        cgGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAg                                                                                             >  1:3337613/1‑101 (MQ=255)
              cGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCcccgc                                                                                       >  2:4221672/1‑101 (MQ=255)
                tGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCGCCTCAGCcccgccc                                                                                     <  1:2502718/101‑1 (MQ=255)
                tGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCcccgccc                                                                                     <  1:1497647/101‑1 (MQ=255)
                 ggATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCcccgcccg                                                                                    >  1:3252541/1‑101 (MQ=255)
                  gATCGAAACTGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCccgcccgc                                                                                   <  2:2099349/101‑1 (MQ=255)
                   aTCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCt                                                                                  <  2:173490/101‑1 (MQ=255)
                    tCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTg                                                                                 >  2:3760835/1‑101 (MQ=255)
                     cGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGt                                                                                >  2:1029827/1‑101 (MQ=255)
                        aaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGtctc                                                                             >  2:4040391/1‑101 (MQ=255)
                          cGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCAc                                                                           <  1:2057282/101‑1 (MQ=255)
                            gtgtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCa                                                                         >  1:2451852/1‑101 (MQ=255)
                             tgtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCaa                                                                        >  2:250261/1‑101 (MQ=255)
                               tCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACa                                                                      >  2:2128879/1‑101 (MQ=255)
                                ccAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAg                                                                     <  2:3053289/101‑1 (MQ=255)
                                ccAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAg                                                                     >  1:2646569/1‑101 (MQ=255)
                                 cAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGt                                                                    >  1:2544426/1‑101 (MQ=255)
                                 cAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGt                                                                    <  2:3621600/101‑1 (MQ=255)
                                 cAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGt                                                                    <  1:1718361/101‑1 (MQ=255)
                                  aGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTc                                                                   >  1:3568843/1‑101 (MQ=255)
                                  aGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTc                                                                   >  1:1022223/1‑101 (MQ=255)
                                   gCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCg                                                                  <  2:1493588/101‑1 (MQ=255)
                                    cGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGc                                                                 <  2:26196/101‑1 (MQ=255)
                                     gTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCt                                                                <  1:2256378/101‑1 (MQ=255)
                                     gTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCt                                                                <  2:1650976/101‑1 (MQ=255)
                                      tCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTc                                                               >  1:3165278/1‑101 (MQ=255)
                                      tCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTc                                                               <  2:3348905/101‑1 (MQ=255)
                                      tCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTc                                                               >  1:1679613/1‑101 (MQ=255)
                                       cacaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcc                                                              >  2:1112100/1‑101 (MQ=255)
                                        ccaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccc                                                             <  1:1167841/100‑1 (MQ=255)
                                         caTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccc                                                            >  2:3893308/1‑101 (MQ=255)
                                         caTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccc                                                            <  2:2769096/101‑1 (MQ=255)
                                         caTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccc                                                            <  2:248999/101‑1 (MQ=255)
                                          aTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccccc                                                           >  1:3854482/1‑101 (MQ=255)
                                           tGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccccc                                                          <  1:1192517/101‑1 (MQ=255)
                                           tGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccccc                                                          <  1:2162703/101‑1 (MQ=255)
                                             cAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcgc                                                        <  2:4111383/101‑1 (MQ=255)
                                              aGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcgcg                                                       <  2:2239848/101‑1 (MQ=255)
                                                  gcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCggggg                                                   >  2:3950283/1‑101 (MQ=255)
                                                  gcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCggggg                                                   <  2:773503/101‑1 (MQ=255)
                                                   cgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGc                                                  <  2:1011216/101‑1 (MQ=255)
                                                      cGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTg                                               <  2:1272347/101‑1 (MQ=255)
                                                       gCATCGCCATGTCGCACCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTgg                                              <  2:3697820/101‑1 (MQ=255)
                                                        ccatcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGCCAAGGCCGCAGCAGCGGCTGCGGTCGCTCCCCCCGCGGGGGCGTgg                                              <  1:1844115/92‑1 (MQ=0)
                                                         aTCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGAt                                            >  2:2337361/1‑101 (MQ=255)
                                                           tcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGCCAAGGCCGCAGCAGCGGCTGCGGTCGCTCCCCCCGCGGGGGCGGGGATc                                           >  2:3269641/7‑101 (MQ=0)
                                                            gCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                         >  2:269164/1‑101 (MQ=255)
                                                            gCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                         <  2:1584742/101‑1 (MQ=255)
                                                             ccATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        >  2:161301/1‑101 (MQ=255)
                                                              cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       >  1:3231832/1‑101 (MQ=255)
                                                              cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       <  1:1083539/101‑1 (MQ=255)
                                                                tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACATTCGCTCCCCCCCCGTCGGCTTGGATTGGAAct                                     >  1:680690/1‑101 (MQ=25)
                                                                tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                     >  2:546195/1‑100 (MQ=255)
                                                                 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                    >  1:1324551/1‑101 (MQ=255)
                                                                  tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActct                                   >  1:4098713/1‑101 (MQ=255)
                                                                   cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTa                                  <  1:1872/101‑1 (MQ=255)
                                                                   cGCCCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTa                                  <  1:810132/101‑1 (MQ=255)
                                                                     ccccccccGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATg                                <  2:310320/99‑1 (MQ=255)
                                                                     cTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGTCACCTCAGCCCCGCCCGCTGTCTCACCAACGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATg                                <  1:2843968/101‑1 (MQ=255)
                                                                      tCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgg                               >  1:1775371/1‑101 (MQ=255)
                                                                       ccccccGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATggg                              >  1:3984936/1‑101 (MQ=255)
                                                                         ccccGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggag                            <  2:4018042/101‑1 (MQ=255)
                                                                         ccccGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggag                            <  2:418740/101‑1 (MQ=255)
                                                                               gggggCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTCTATgggaggctagg                      >  1:874820/1‑101 (MQ=255)
                                                                                ggggCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggg                     <  1:1789445/101‑1 (MQ=255)
                                                                                ggggCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggg                     <  1:3429612/101‑1 (MQ=255)
                                                                                 gggCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggga                    <  2:3165039/101‑1 (MQ=255)
                                                                                  ggCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggag                   <  1:2953589/101‑1 (MQ=255)
                                                                                  ggCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggag                   <  1:1601814/101‑1 (MQ=255)
                                                                                   gCGTGGATCGAAGC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggagg                  <  2:638287/101‑1 (MQ=255)
                                                                                   gCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGATCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggagg                  <  2:695259/101‑1 (MQ=255)
                                                                                     gTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggct                <  1:2914328/101‑1 (MQ=255)
                                                                                      tGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggcta               >  1:341406/1‑101 (MQ=255)
                                                                                        gATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACg             >  1:446694/1‑101 (MQ=255)
                                                                                        gATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACg             <  2:3337368/101‑1 (MQ=255)
                                                                                        gATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACg             >  1:1267256/1‑101 (MQ=255)
                                                                                        gATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACg             >  2:3822134/1‑101 (MQ=255)
                                                                                        gATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACg             <  1:580225/101‑1 (MQ=255)
                                                                                        gATCAAACC.CCAGCTCCTCAGCCCCGCCCGCTGTCCCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACg             <  2:4033444/101‑1 (MQ=37)
                                                                                         aTCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGc            >  2:509876/1‑101 (MQ=255)
                                                                                           cGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAc          >  1:478974/1‑101 (MQ=255)
                                                                                           cGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAc          >  1:3305400/1‑101 (MQ=255)
                                                                                            gAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAcc         >  2:3877245/1‑101 (MQ=255)
                                                                                            gAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAcc         >  2:2015136/1‑101 (MQ=255)
                                                                                             aaaC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCt        >  1:2099490/1‑101 (MQ=255)
                                                                                             aaaC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCt        <  1:4201563/101‑1 (MQ=255)
                                                                                             aaaC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCt        >  2:809820/1‑101 (MQ=255)
                                                                                              aaC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCtt       <  2:1324451/101‑1 (MQ=255)
                                                                                               aC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTc      >  2:3372455/1‑101 (MQ=255)
                                                                                                  ccGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaa    <  2:2897231/101‑1 (MQ=255)
                                                                                                   cGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaa   <  1:3660533/101‑1 (MQ=255)
                                                                                                    ggCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaaa  <  2:1226032/101‑1 (MQ=255)
                                                                                                    ggCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaaa  <  1:2316782/101‑1 (MQ=255)
                                                                                                     |                                                                                                   
TCCCCCCGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAA  >  NC_014034/1397771‑1397970

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG