Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,890 T→A 2.1% intergenic (+194/+1704) RCAP_rcc01307 → / ← RCAP_rcc01309 hypothetical protein/IS66 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,8900T→A 2.1% 3.0 ‑0.5 ‑0.1191intergenic (+194/+1704)RCAP_rcc01307/RCAP_rcc01309hypothetical protein/IS66 family transposase
Reads supporting (aligned to +/- strand):  new base (1/3):  ref base (89/98):  total (90/101)
Fisher's exact test strand distribution p-value = 6.24e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.88e-01

Only 100 of 207 total aligned reads displayed.

ATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1397790‑1397990
                                                                                                     |                                                                                                    
aTCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCt                                                                                                      <  2:173490/101‑1 (MQ=255)
  cGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGt                                                                                                    <  1:295165/101‑1 (MQ=255)
   gAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGtc                                                                                                   >  2:3884056/1‑101 (MQ=255)
    aaaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGtct                                                                                                  <  1:2765484/101‑1 (MQ=255)
     aaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGtctc                                                                                                 >  2:4040391/1‑101 (MQ=255)
     aaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCCCCCCCCGCGGGGGCGTGGATCGAACC.CCGGCACCTCAGCCCCGCCCGCTGtctc                                                                                                 <  1:1029918/101‑1 (MQ=255)
        ggTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCAcc                                                                                              >  1:384789/1‑101 (MQ=255)
         gtgtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCa                                                                                             >  1:2451852/1‑101 (MQ=255)
          tgtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCaa                                                                                            >  1:2842768/1‑101 (MQ=255)
           gtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAAc                                                                                           >  1:2897450/1‑101 (MQ=255)
            tCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACa                                                                                          >  2:2128879/1‑101 (MQ=255)
             ccAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAg                                                                                         <  2:658790/101‑1 (MQ=255)
             ccAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAg                                                                                         <  2:3053289/101‑1 (MQ=255)
              cAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGt                                                                                        <  1:1718361/101‑1 (MQ=255)
              cAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGt                                                                                        <  1:3884315/101‑1 (MQ=255)
               aGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTc                                                                                       <  2:3922422/101‑1 (MQ=255)
               aGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCAAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTc                                                                                       <  2:736749/101‑1 (MQ=255)
                gCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCg                                                                                      <  2:1493588/101‑1 (MQ=255)
                 cGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGc                                                                                     >  2:3226105/1‑101 (MQ=255)
                   tCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTc                                                                                   >  1:1679613/1‑101 (MQ=255)
                   tCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTc                                                                                   <  2:3348905/101‑1 (MQ=255)
                   tCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCAGCCCGCTGTCTCACCAACAGTCGCTc                                                                                   >  1:3549986/1‑101 (MQ=255)
                     ccaTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccc                                                                                 <  1:1167841/100‑1 (MQ=255)
                      caTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccc                                                                                <  2:248999/101‑1 (MQ=255)
                        tGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccccc                                                                              <  1:1192517/101‑1 (MQ=255)
                         gCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcg                                                                             >  1:1351767/1‑101 (MQ=255)
                          cAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcgc                                                                            <  2:4111383/101‑1 (MQ=255)
                           aGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcgcg                                                                           <  2:2239848/101‑1 (MQ=255)
                               gcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCggggg                                                                       >  2:3950283/1‑101 (MQ=255)
                               gcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCggggg                                                                       <  2:773503/101‑1 (MQ=255)
                                cgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGc                                                                      <  2:1011216/101‑1 (MQ=255)
                                   cGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTg                                                                   <  2:1272347/101‑1 (MQ=255)
                                    gCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTgg                                                                  <  2:3856271/101‑1 (MQ=255)
                                    gCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTgg                                                                  <  2:3299870/101‑1 (MQ=255)
                                     ccatcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGCCAAGGCCGCAGCAGCGGCTGCGGTCGCTCCCCCCGCGGGGGCGTgg                                                                  <  1:1844115/92‑1 (MQ=0)
                                     cATCGCCATGTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                 <  2:2544235/101‑1 (MQ=255)
                                      aTCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                >  2:2337361/1‑101 (MQ=255)
                                       tCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                               >  2:2018321/1‑101 (MQ=255)
                                        tcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGCCAAGGCCGCAGCAGCGGCTGCGGTCGCTCCCCCCGCGGGGGGGTGGATc                                                               >  1:702669/7‑101 (MQ=0)
                                        cGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                              >  2:457184/1‑101 (MQ=255)
                                         gCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                             <  2:1584742/101‑1 (MQ=255)
                                         gCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                             >  2:269164/1‑101 (MQ=255)
                                          ccATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                            <  2:3730108/101‑1 (MQ=255)
                                          ccATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                            >  2:161301/1‑101 (MQ=255)
                                            aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAACTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGGATGGATCGAAAc                                                          >  2:633275/1‑101 (MQ=255)
                                             tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                         >  2:546195/1‑100 (MQ=255)
                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                                        >  1:1324551/1‑101 (MQ=255)
                                               tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActct                                                       >  1:4098713/1‑101 (MQ=255)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTAt                                                     <  2:4022047/101‑1 (MQ=255)
                                                  ccccccccGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATg                                                    <  2:310320/99‑1 (MQ=255)
                                                  cTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATg                                                    <  1:1138179/101‑1 (MQ=255)
                                                  cTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATg                                                    >  1:2375949/1‑101 (MQ=255)
                                                   cccccccGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCCCCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgg                                                   <  1:2531232/100‑1 (MQ=255)
                                                      ccccGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggag                                                <  2:418740/101‑1 (MQ=255)
                                                      ccccGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggag                                                >  1:3182061/1‑101 (MQ=255)
                                                        ccGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggc                                              <  1:222946/101‑1 (MQ=255)
                                                         cgcgGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggct                                             <  1:2046257/101‑1 (MQ=255)
                                                           cgGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctag                                           <  2:3779565/101‑1 (MQ=255)
                                                            gggggCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagg                                          <  1:1745539/101‑1 (MQ=255)
                                                            gggggCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACTCTATgggaggctagg                                          >  1:874820/1‑101 (MQ=255)
                                                             ggggCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggg                                         <  1:1789445/101‑1 (MQ=255)
                                                              gggCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggga                                        <  2:3165039/101‑1 (MQ=255)
                                                                gCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGNGGATCGAAACTCTATgggaggctagggagg                                      >  1:454049/1‑101 (MQ=255)
                                                                  gTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggct                                    >  2:1679204/1‑101 (MQ=255)
                                                                  gTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggct                                    >  1:3124924/1‑101 (MQ=255)
                                                                   tGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggcta                                   >  1:341406/1‑101 (MQ=255)
                                                                     gATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACg                                 <  2:3337368/101‑1 (MQ=255)
                                                                       tCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCa                               <  2:2780609/101‑1 (MQ=255)
                                                                        cGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAc                              >  1:3305400/1‑101 (MQ=255)
                                                                         gAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCCcc                             >  2:191828/1‑101 (MQ=255)
                                                                         gAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAcc                             >  2:2626769/1‑101 (MQ=255)
                                                                         aaccc.ccGGC.CCTCCGCCCCCGCCGCTGTCTCCCCAACAGTCGGCCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCt                            <  2:108055/97‑1 (MQ=25)
                                                                          aaaC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCt                            <  1:1600305/101‑1 (MQ=255)
                                                                          aaaC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCt                            >  2:809820/1‑101 (MQ=255)
                                                                          aaaC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCt                            <  1:4201563/101‑1 (MQ=255)
                                                                           aaC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCtt                           >  1:3248937/1‑101 (MQ=255)
                                                                           aaC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCtt                           <  2:1324451/101‑1 (MQ=255)
                                                                            aC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTc                          <  1:227382/101‑1 (MQ=255)
                                                                            aC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTc                          >  2:3372455/1‑101 (MQ=255)
                                                                             c.ccGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCa                         <  2:3984676/101‑1 (MQ=255)
                                                                               ccGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaa                        >  1:2629174/1‑101 (MQ=255)
                                                                               ccGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaa                        >  1:1981734/1‑101 (MQ=255)
                                                                                cGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaa                       <  1:3660533/101‑1 (MQ=255)
                                                                                 ggCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaaa                      <  2:1226032/101‑1 (MQ=255)
                                                                                   cACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGt                    <  2:2842549/101‑1 (MQ=255)
                                                                                     ccTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCg                  <  2:3421903/101‑1 (MQ=255)
                                                                                        cAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTc               <  1:589473/101‑1 (MQ=255)
                                                                                        cAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTc               >  1:1019602/1‑101 (MQ=255)
                                                                                          gCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGAATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGccacc             >  2:3662864/1‑97 (MQ=255)
                                                                                           gcccgcccgCTGTCCCCTCAACAGTCGCGTCCCCCGCGGGGGCGTGAATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTcccc            <  2:11328/100‑1 (MQ=255)
                                                                                            cccgcccgCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTccccc           <  2:1510200/101‑1 (MQ=255)
                                                                                            cccgcccgCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTccccc           >  1:3582635/1‑101 (MQ=255)
                                                                                            cccgcccgCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTcccac           >  1:2060677/1‑99 (MQ=255)
                                                                                             ccgcccgcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGNGGCTACGCACCTTCAAAAGTCGCTccccac          >  1:441183/1‑99 (MQ=255)
                                                                                              cgcccgcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCcg         <  1:655594/101‑1 (MQ=255)
                                                                                              cgcccgcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCcg         <  1:2129024/101‑1 (MQ=255)
                                                                                               gcccgcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCcgc        >  1:1238582/1‑101 (MQ=255)
                                                                                                 ccgcTGTCTCACCAACAGTCGCTCCCCCCGNGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCgg      <  1:1306738/101‑1 (MQ=255)
                                                                                                     tGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGGAACTCTATGGGAGGCTAGGGAGGCTACGTACCTTCAAAAGTCGCACCCCCCGCGGGGGc  >  2:1667717/1‑101 (MQ=255)
                                                                                                     tGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGc  <  1:809898/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
ATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1397790‑1397990

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG