Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,412 C→G 12.8% intergenic (‑191/‑767) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,4120C→G12.8% 69.7 ‑1.4 ‑0.0245intergenic (‑191/‑767)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (17/14):  ref base (92/119):  total (110/135)
Fisher's exact test strand distribution p-value = 2.53e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.58e-01

Only 100 of 254 total aligned reads displayed.

AAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1434312‑1434510
                                                                                                       |                                                                                                    
 aaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCt                                                                                                     <  1:1072334/101‑1 (MQ=255)
 aaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCt                                                                                                     >  2:2193152/1‑101 (MQ=255)
  aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCtt                                                                                                    <  2:3027554/101‑1 (MQ=255)
  aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAAC..GTCCCCGCTCTGCTTCACG.GCTGCGTCAACCtt                                                                                                    <  1:1183836/101‑1 (MQ=255)
   cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..g                                                                                                 <  2:84287/101‑1 (MQ=255)
        tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCt                                                                                            <  1:1020382/101‑1 (MQ=255)
        tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCt                                                                                            <  2:2482548/101‑1 (MQ=255)
         gctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTc                                                                                           >  2:11699/1‑101 (MQ=255)
          ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAGAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcc                                                                                          >  2:3553652/1‑101 (MQ=255)
          ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcc                                                                                          <  2:3425182/101‑1 (MQ=255)
          ctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcc                                                                                          <  2:2379890/101‑1 (MQ=255)
            ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcccc                                                                                        <  2:3511012/101‑1 (MQ=255)
            ttGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcccc                                                                                        <  1:1459767/101‑1 (MQ=255)
              gggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTcccccc                                                                                      <  2:633792/101‑1 (MQ=255)
               ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTccccccc                                                                                     >  1:1339425/1‑100 (MQ=255)
                   gAACCCCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCggg                                                                                 <  2:3111987/101‑1 (MQ=255)
                   gAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCggg                                                                                 >  2:1353979/1‑101 (MQ=255)
                    aaCACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCgggg                                                                                >  2:964452/1‑101 (MQ=255)
                      cacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGc                                                                              <  2:3249262/101‑1 (MQ=255)
                       acCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCg                                                                             <  1:4221537/101‑1 (MQ=255)
                       acCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCg                                                                             >  1:231090/1‑101 (MQ=255)
                        ccTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGt                                                                            <  2:2065677/101‑1 (MQ=255)
                            aTCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                        >  1:2363117/1‑101 (MQ=255)
                             tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       <  1:3705856/101‑1 (MQ=255)
                             tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       <  2:3930046/101‑1 (MQ=255)
                             tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       <  2:2740771/101‑1 (MQ=255)
                             tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       >  2:2355316/1‑101 (MQ=255)
                             cgggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTgg                                                                          <  1:1363053/92‑1 (MQ=2)
                              ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  2:3854930/101‑1 (MQ=255)
                                tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    <  2:4172298/101‑1 (MQ=255)
                                gctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       <  1:4103861/95‑1 (MQ=2)
                                 ttatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  2:512343/96‑1 (MQ=2)
                                 gctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   <  1:3572215/101‑1 (MQ=255)
                                 gctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCAGCGGCATCCTT..TTCGCTCCCCCCGCGGGGGGGTGGATCGaaa                                                                   >  2:1683385/1‑101 (MQ=25)
                                   tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                                 <  1:322593/101‑1 (MQ=255)
                                   tgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAaca                                                                 >  2:44880/1‑101 (MQ=255)
                                   atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    >  1:3139733/4‑101 (MQ=2)
                                    gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                                >  1:594455/1‑101 (MQ=255)
                                    gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                                >  1:2545627/1‑101 (MQ=255)
                                     ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACg                                                               <  2:387163/101‑1 (MQ=255)
                                     cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                  <  2:292905/100‑1 (MQ=2)
                                         gcgccccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctg                                                              <  1:648318/98‑5 (MQ=2)
                                          cTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgc                                                             >  2:2861610/1‑96 (MQ=2)
                                          cTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTg                                                          >  1:2442518/1‑101 (MQ=255)
                                              ccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgac                                                         >  1:3279536/1‑92 (MQ=2)
                                              ccccGCGGGGGCGTGGATCGAAAC..TTGTCTGACGCGCTCATCA.GC.ACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCggcaaggac                                                       <  1:3692200/101‑8 (MQ=37)
                                              ccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATg                                                      >  1:2435734/1‑101 (MQ=255)
                                               cccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgaca                                                        <  1:3223744/101‑11 (MQ=0)
                                               cccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctgaca                                                        <  1:3862991/101‑11 (MQ=0)
                                               cccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATgg                                                     <  1:4223063/101‑1 (MQ=255)
                                               cccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATgg                                                     <  1:3793530/101‑1 (MQ=255)
                                                ccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                                                    <  1:491141/101‑1 (MQ=255)
                                                ccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                                                    >  2:639453/1‑101 (MQ=255)
                                                 cgcgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcg                                                   <  2:3983981/101‑1 (MQ=255)
                                                  gcgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcgc                                                  >  2:333193/1‑101 (MQ=255)
                                                   cgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                                 <  1:2156382/101‑1 (MQ=255)
                                                   cgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                                 <  1:1266052/101‑1 (MQ=255)
                                                    gggggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACTGATTGCATGGCGCCg                                                <  2:3704748/101‑1 (MQ=255)
                                                    gggggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                                                >  1:1937812/1‑101 (MQ=255)
                                                      gggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                              >  2:3634195/1‑101 (MQ=255)
                                                        gCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAggg                                            <  2:2012616/101‑1 (MQ=255)
                                                         cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGa                                           <  1:291094/101‑1 (MQ=255)
                                                         cGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GGCGCTCCCCCCGCGGGGGGGTGGATCGAAACACGGATGGCATGGCGCNGANGGa                                           >  1:710392/1‑101 (MQ=255)
                                                          gTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGCGGGAc                                          >  2:2639732/1‑101 (MQ=255)
                                                           tGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACa                                         <  1:2057354/101‑1 (MQ=255)
                                                             gATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAgg                                       >  1:533225/1‑101 (MQ=255)
                                                                 gAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                   <  2:2116587/101‑1 (MQ=255)
                                                                  aaaC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg                                  <  1:174148/101‑1 (MQ=255)
                                                                  aaaC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg                                  >  1:851907/1‑101 (MQ=255)
                                                                   aaC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                                 <  1:2782214/101‑1 (MQ=255)
                                                                   aaC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                                 <  2:1646461/101‑1 (MQ=255)
                                                                   aaC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                                 >  2:1476012/1‑101 (MQ=255)
                                                                    aC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCa                                <  1:3008264/101‑1 (MQ=255)
                                                                    aC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCa                                <  1:4234840/101‑1 (MQ=255)
                                                                    aC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCACGGGACAGGCTTTGCa                                >  2:517457/1‑101 (MQ=255)
                                                                     c..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAg                               <  2:3570259/101‑1 (MQ=255)
                                                                     c..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAg                               <  2:4096162/101‑1 (MQ=255)
                                                                        gTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt                              >  1:3063356/1‑101 (MQ=255)
                                                                        gTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGt                              >  1:927974/1‑101 (MQ=255)
                                                                         tctcCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGGGCCGAGGGACAGGCTTTGCAGTc                             >  1:470080/1‑101 (MQ=255)
                                                                          ctcCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCg                            >  1:339138/1‑101 (MQ=255)
                                                                             cGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTc                         <  1:2942963/101‑1 (MQ=255)
                                                                               ctctGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCCTGGCGCCGAGGGACAGGCTTTGCAGTCGCTccc                       >  1:3644826/1‑101 (MQ=255)
                                                                               ctctGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccc                       >  2:1854485/1‑101 (MQ=255)
                                                                                 ctGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccccc                     >  1:1154336/1‑101 (MQ=255)
                                                                                 ctGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccccc                     >  1:3915951/1‑101 (MQ=255)
                                                                                  tGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc                    <  1:944676/101‑1 (MQ=255)
                                                                                   gCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcg                   <  1:2738112/101‑1 (MQ=255)
                                                                                     ttCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcgcg                 >  2:829229/1‑101 (MQ=255)
                                                                                        ccG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCgggg              <  2:1197310/100‑1 (MQ=255)
                                                                                         cG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCggggg             >  2:3079360/1‑101 (MQ=255)
                                                                                          g.gCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGc            >  1:2099178/1‑101 (MQ=40)
                                                                                              tGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCTTg         <  1:2414160/101‑1 (MQ=38)
                                                                                               gCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTgg        <  2:3821093/101‑1 (MQ=37)
                                                                                                   cAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGGTCg    >  2:3904619/1‑101 (MQ=17)
                                                                                                   cAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCg    <  1:2639938/101‑1 (MQ=32)
                                                                                                    aaCCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa   >  2:175288/1‑101 (MQ=31)
                                                                                                    aaCCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa   >  2:1939007/1‑101 (MQ=31)
                                                                                                     aCCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa  <  2:2442334/101‑1 (MQ=11)
                                                                                                     aCCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaa  >  1:2693241/1‑101 (MQ=11)
                                                                                                       |                                                                                                    
AAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTT..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1434312‑1434510

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG