Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,607,933 G→C 100% intergenic (+203/‑144) RCAP_rcc01484 → / → murD hypothetical protein/UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,607,9330G→C100.0% 442.4 120intergenic (+203/‑144)RCAP_rcc01484/murDhypothetical protein/UDP‑N‑acetylmuramoylalanine‑‑D‑glutamate ligase
Reads supporting (aligned to +/- strand):  new base (40/79):  ref base (1/0):  total (41/79)

Only 100 of 149 total aligned reads displayed.

CGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGGAATGCGGGCGAGACCGGGCCGGACCGGCGCCTGTCTTCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCTTGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTGGGCCG  >  NC_014034/1607833‑1608031
                                                                                                    |                                                                                                  
 gccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGcc                                                                                                <  1:2752341/101‑1 (MQ=255)
 gccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGcc                                                                                                >  2:2426582/1‑101 (MQ=255)
 gccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGcc                                                                                                <  1:4041346/101‑1 (MQ=255)
  ccgcGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCt                                                                                               <  2:487605/98‑1 (MQ=255)
  ccACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGccc                                                                                               >  2:828854/1‑100 (MQ=255)
   cACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTg                                                                                              <  1:693357/101‑1 (MQ=255)
      gCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGG.ATGCGGGC.AGACCGGGCC.GACCGCCGCCCGTCt                                                                                           >  1:3420295/1‑101 (MQ=255)
      ccccGCATTGTGGCCGCCGCCCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCt                                                                                           <  2:3117382/100‑1 (MQ=255)
       cccGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCCGCCC.GGGAATGCGGG.GCGACCGGGCCGCACC.CCGCCTGTccc                                                                                          >  1:332308/1‑99 (MQ=255)
        ccGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtcc                                                                                         <  1:1988701/101‑3 (MQ=255)
         cggaTTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTCtccg                                                                                        <  1:4188868/98‑4 (MQ=255)
           cATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGAc                                                                                     >  1:489814/1‑101 (MQ=255)
           cATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCCGTCTCccac                                                                                      >  1:1636439/1‑99 (MQ=255)
            aTTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCCGTC.TCCGAcc                                                                                    >  1:403991/1‑101 (MQ=255)
              tgtgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCtt                                                                                  <  1:3999433/101‑1 (MQ=255)
               gtgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCttt                                                                                 <  2:484224/101‑1 (MQ=255)
               gtgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCCGTC.TCCGACCctt                                                                                 >  1:2975301/1‑101 (MQ=255)
                tgGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCtttt                                                                                >  2:959604/1‑101 (MQ=255)
                gggCCTCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCtttt                                                                                <  1:2318164/100‑1 (MQ=255)
                   ccgccgCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTccc                                                                             >  2:2506167/1‑101 (MQ=255)
                   cccccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTccc                                                                             <  2:1164093/98‑1 (MQ=255)
                    cgccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCa                                                                            <  2:2583727/101‑1 (MQ=255)
                    cctcatatcccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCCCCGCCTGTC.TCCGACCTTTTCCCa                                                                            >  2:3817041/9‑101 (MQ=255)
                     gccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAg                                                                           <  2:2975079/101‑1 (MQ=255)
                     gccgcGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAg                                                                           >  2:2840292/1‑101 (MQ=255)
                       cgcgCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCCGCg                                                                         >  1:1081449/1‑101 (MQ=255)
                         cgcCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGa                                                                       <  2:621701/101‑1 (MQ=255)
                         cgcCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGa                                                                       >  2:3875482/1‑101 (MQ=255)
                         cgcCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGa                                                                       <  1:1950785/101‑1 (MQ=255)
                         ccccccAGACCGCGCCCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGa                                                                       <  2:4013413/99‑1 (MQ=255)
                          cccccAGAACGCGCCCCCCGGCCCCTCAAGCATGGCGGCGGCCC.GGGAATGCGGGC.AGCCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGAt                                                                      <  2:327178/100‑1 (MQ=255)
                           ccccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATc                                                                     <  2:3459715/101‑1 (MQ=255)
                            cccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATcc                                                                    <  2:3739886/101‑1 (MQ=255)
                             ccggAACGCGCGCCCCGGCCCCTCAAGGATGGCGCCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATccc                                                                   <  2:1024050/98‑1 (MQ=255)
                             ccAGAACGCGCGCCCGGGCCCCTCAAGGATGGGGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATccc                                                                   <  2:3272908/101‑1 (MQ=255)
                             ccAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATccc                                                                   <  2:953159/101‑1 (MQ=255)
                              cAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcg                                                                  <  2:2291607/101‑1 (MQ=255)
                              cAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcg                                                                  <  1:219598/101‑1 (MQ=255)
                              cAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcg                                                                  <  2:2629758/101‑1 (MQ=255)
                              cAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcg                                                                  <  1:1100367/101‑1 (MQ=255)
                              cAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcg                                                                  <  1:2500642/101‑1 (MQ=255)
                               aGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgc                                                                 >  1:3936332/1‑101 (MQ=255)
                               aGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCcgc                                                                 >  1:1937717/1‑101 (MQ=255)
                                 aaCGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGa                                                               <  2:2390349/101‑1 (MQ=255)
                                 aaCGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGa                                                               <  2:1286632/101‑1 (MQ=255)
                                  ccgcgcgCCCCGCCCCCTCAAGGATGTCGGCGGCCC.GGGAATGCGGGC.AGTCCGGG.CGGCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGAc                                                              <  2:1246101/100‑1 (MQ=255)
                                  aCGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGAc                                                              <  1:1555887/101‑1 (MQ=255)
                                   cgcgcgCCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACg                                                             <  2:4191696/101‑1 (MQ=255)
                                    gcgcgcCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACgg                                                            >  1:472881/1‑101 (MQ=255)
                                        gcCCCGGCCCCTCAAGGATGGCGGCGGCCCCGGG.ATGCGGGC.AGACCGGGCC.GACCGGCGCCTGTC.TCCGACCTTTTCCCCGCGGATCCCGCGAGggggag                                                        >  2:1036270/1‑99 (MQ=255)
                                        gcCCCGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTg                                                        >  1:2466276/1‑101 (MQ=255)
                                         ccccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGGCGGGGGGc                                                       >  2:3454209/1‑101 (MQ=255)
                                         ccccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGc                                                       <  2:2170301/101‑1 (MQ=255)
                                          cccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCg                                                      >  1:1634393/1‑101 (MQ=255)
                                           ctggCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGCCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGa                                                     <  1:3629263/99‑1 (MQ=255)
                                           ccGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGa                                                     >  1:3874256/1‑101 (MQ=255)
                                            cGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGAc                                                    >  2:3562983/1‑101 (MQ=255)
                                            cGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGAc                                                    <  1:2949613/101‑1 (MQ=255)
                                            cGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCgcc                                                    >  2:2562143/1‑99 (MQ=255)
                                            cGGCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCGAc                                                    >  1:3837853/1‑101 (MQ=255)
                                             ggCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACg                                                   <  2:1870770/101‑1 (MQ=255)
                                             ggCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGGGCggag                                                   >  2:3629010/1‑98 (MQ=255)
                                              gCCCCTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCCCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGc                                                  >  1:3394647/1‑101 (MQ=255)
                                                cccTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCt                                                <  1:1288737/101‑1 (MQ=255)
                                                 ccTCAAGGATGCCGGCGGCCC.GGGAATGCGGGC.AGACCGGG.CGGGCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtg                                               <  1:3231825/101‑2 (MQ=255)
                                                  cTCAAGGATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgc                                              <  2:1767726/101‑3 (MQ=255)
                                                   tCAAGGATGGCGGCGGCCC.GGGAATGCGGGCG.GACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCtgcg                                             <  1:157541/101‑4 (MQ=255)
                                                       ggAGGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGgcgc                                        <  1:80805/101‑1 (MQ=255)
                                                        gATGGCGGCGGCCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGcgcc                                       <  1:3984084/101‑1 (MQ=255)
                                                           ggcggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCgccgcc                                    >  1:1267746/1‑101 (MQ=255)
                                                            gcggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGccc                                   <  1:2224571/101‑1 (MQ=255)
                                                             gggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCg                                  <  1:333599/100‑1 (MQ=255)
                                                             gggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCg                                  <  2:1963808/100‑1 (MQ=255)
                                                              ggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGa                                 >  1:3775511/1‑101 (MQ=255)
                                                              ggcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGa                                 <  1:655941/101‑1 (MQ=255)
                                                               gcggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGaa                                >  2:3707763/1‑101 (MQ=255)
                                                                 ggcCC.GGGAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGACAg                              >  2:3003848/1‑101 (MQ=255)
                                                                     ccgggAAGGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAgggg                           <  2:3936072/99‑1 (MQ=255)
                                                                      cgggAATGCGGGC.AGCCCGGGCC.CACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAggggg                          <  1:418410/100‑1 (MQ=255)
                                                                      cgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAGagggg                          >  2:3977831/2‑101 (MQ=255)
                                                                      cgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAggggg                          <  2:489757/100‑1 (MQ=255)
                                                                      cgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAggggg                          >  1:549348/2‑101 (MQ=255)
                                                                      cgggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAgcggg                          >  2:1307088/2‑101 (MQ=255)
                                                                       gggAATGCGGGC.AGCCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAgggggg                         <  2:752124/101‑1 (MQ=255)
                                                                       gggAATGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGACTGCGACGCC.TGCGGCGCCGCCCGAAAgggggg                         >  1:3646819/1‑101 (MQ=255)
                                                                           aTGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTgc                     <  1:1004141/101‑1 (MQ=255)
                                                                           aTGCGGGC.AGACCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTgc                     >  2:2653209/1‑101 (MQ=255)
                                                                               cgggcagaCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTgcgcg                  <  2:1976636/96‑1 (MQ=255)
                                                                                   cagcccGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGcccc              <  2:3216877/97‑1 (MQ=255)
                                                                                   cagaCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGcccc              <  1:2134308/100‑1 (MQ=255)
                                                                                    agaCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGccccc             <  2:3873997/101‑1 (MQ=255)
                                                                                      aCCGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGccccccc           >  2:2354490/1‑100 (MQ=255)
                                                                                       ccGGGCC.GCCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCctc          <  2:679940/101‑1 (MQ=255)
                                                                                        cGGGCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCctct         <  2:3786267/101‑1 (MQ=255)
                                                                                         gggCC.GACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCCCtt        >  1:2334439/1‑101 (MQ=255)
                                                                                           ggccgACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGCGCGACGCC.TGCGGCGCCGCCCGCAGCAGCGGCAGCGCGccccccccccc       >  2:1467115/5‑96 (MQ=255)
                                                                                               gcccGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTGGTcc   <  2:3995708/99‑1 (MQ=38)
                                                                                               gcccGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTGGTcc   <  1:1655572/99‑1 (MQ=38)
                                                                                               gACCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTGGTcc   >  2:2903541/1‑101 (MQ=38)
                                                                                                aCCGCCGCCTGTC.TCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCC.TGCGGCGCCGCCCGAAAGGGGGGCGGCGCCACCCCCCCTTggttcg  >  1:1866039/1‑97 (MQ=255)
                                                                                                    |                                                                                                  
CGCCACGCCCGCATTGTGGCCGCCGCGCCCCAGAACGCGCGCCCCGGCCCCTCAAGGATGGCGGCGGCCCGGGGAATGCGGGCGAGACCGGGCCGGACCGGCGCCTGTCTTCCGACCTTTTCCCAGCGGATCCCGCGACGGGGTGCGACGCCTTGCGGCGCCGCCCGAAAGGGGGGCTGCGCGCCCCCCTCTTGGGCCG  >  NC_014034/1607833‑1608031

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG