Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,612,426 C→A 3.2% intergenic (+104/‑257) purN → / → rnd2 phosphoribosylglycinamide formyltransferase/ribonuclease D

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,612,4260C→A 3.2% 4.8 ‑3.8 ‑0.4153intergenic (+104/‑257)purN/rnd2phosphoribosylglycinamide formyltransferase/ribonuclease D
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (68/80):  total (73/80)
Fisher's exact test strand distribution p-value = 2.30e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.90e-01

Only 100 of 180 total aligned reads displayed.

CGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCGGGGAAAGG  >  NC_014034/1612326‑1612525
                                                                                                     |                                                                                                    
cGATCCGCTGATCTGACCGAGGCCTTCGCCGAGGTCACGCGACACATTGCCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCcgccccgccc                                                                                                      <  1:3592094/101‑1 (MQ=255)
 gATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCa                                                                                                     >  1:638667/1‑101 (MQ=255)
  acccGCTGATCGGACCGGGCCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCAt                                                                                                    <  2:45843/99‑1 (MQ=255)
   tCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATg                                                                                                   >  2:3118042/1‑101 (MQ=255)
    ccGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATgg                                                                                                  >  1:1342368/1‑101 (MQ=255)
      gCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATggcg                                                                                                >  1:2792029/1‑101 (MQ=255)
       cTGATCTGCCCGGGCCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATggcgg                                                                                               <  1:892415/101‑1 (MQ=255)
        tGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATggcggg                                                                                              >  1:4222839/1‑101 (MQ=255)
         gATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCCCCCGCCCCGCCCATcgcgggg                                                                                             >  1:946290/1‑100 (MQ=255)
           tcggcgcGAGGCCCGCGCCGAGCTCCCGCGACACATGGGCCCCGGGACCGCGGGGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGgcgc                                                                                           <  1:1278985/95‑1 (MQ=14)
           tCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATggcggggcc                                                                                           >  2:1358669/1‑98 (MQ=255)
            cTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCa                                                                                          <  2:599074/101‑1 (MQ=255)
             tGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGTCC.TGCCCCCGCCCCGCCCACggcggggggag                                                                                         >  2:494189/1‑96 (MQ=17)
             tGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGCac                                                                                         >  1:4130365/1‑100 (MQ=255)
               aCCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATggcgggggggttt                                                                                       >  2:1792687/1‑94 (MQ=255)
               aCCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATggcggggggcctt                                                                                       >  1:2252578/1‑94 (MQ=255)
                 cGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCCTTccc                                                                                     >  2:34883/1‑99 (MQ=255)
                   aGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGGATTCgcg.c                                                                                  >  2:3671566/1‑101 (MQ=255)
                    ggCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCCCTCGcg.cc                                                                                 >  1:4075187/1‑101 (MQ=255)
                    ggCCAGCGCCGAGGTCCCGCGACACATGGGCCCCGTGCCCGCCGAGGGCCCAGAGCCCCTCGCCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGcg.cc                                                                                 <  1:4159823/101‑1 (MQ=17)
                     gCCATCGCCGAGGTCACGCGACACTTGGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGcg.ccg                                                                                <  2:2842893/101‑1 (MQ=255)
                      ccATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCAGTCGcc.ccgc                                                                               >  2:4123234/1‑101 (MQ=255)
                       caccGCGGAGGTCCCGCGACACATTGGCCCCGTGACCGCCGGGGGCCCAGGGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCg.ccgcc                                                                              <  1:3931526/98‑1 (MQ=17)
                       cATCGCCGAGGCCCCGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCg.ccgcc                                                                              <  2:3344702/101‑1 (MQ=255)
                       atctcccGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCg.ccgcc                                                                              <  2:2490929/96‑1 (MQ=255)
                          cGCCGGGGTCACGCGACACATTGGCCCCGTGCCCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCggg                                                                           <  1:2175728/101‑1 (MQ=255)
                           gCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGTGCGTTTGCGCCCGCCggg                                                                           >  2:981814/1‑101 (MQ=17)
                            ccGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCCATCCCg.cccccccggc                                                                         >  2:2650930/1‑96 (MQ=17)
                               gggccgcgcgACACATTGGCCCCGTGCCCGCGGGGGCCCCGGAGGCCCCCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCggg                                                                      <  1:3908499/95‑1 (MQ=17)
                                  ccccgcgCCACATTGGCCCCGGGCCCGCCGAGGGCCCGGAGCCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACg                                                                   <  1:1131099/98‑1 (MQ=17)
                                   cACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGGCTTCCCG.CCGCCGGGGGggggggc                                                                  >  1:1578646/1‑97 (MQ=17)
                                   cACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCCGGGGggggggc                                                                  >  2:850555/1‑97 (MQ=255)
                                    aCGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCCTTCGCG.CCCCCCGGGGggggggcc                                                                 >  1:1310750/1‑96 (MQ=17)
                                     cgcgCCCCATTGGCCCCGTCACCGCGGAGGGCCAAGAGCCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAg                                                                <  1:995581/101‑1 (MQ=17)
                                     cgcgACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAg                                                                <  1:1365474/101‑1 (MQ=255)
                                       cgACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGCATTCGCG.CCGCCGGGGGGGGGCGCAGGc                                                              >  1:1746024/1‑101 (MQ=255)
                                       cgACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCCTTCGCG.CCGCCCGGGCGGGGGGCAGGc                                                              >  1:3887733/1‑101 (MQ=17)
                                       cgACACATTGCCCCCGTGCCCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGc                                                              <  2:2791810/101‑1 (MQ=255)
                                       caacacATGGGCCCCGTGCCCCCCGAGGGCCCGGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGc                                                              <  2:1927544/99‑1 (MQ=17)
                                         acacATTGGCCCCGTGCCCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCt                                                            <  1:3729844/101‑1 (MQ=255)
                                          cacaTTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCCTTCGCG.CCCCCCGGGCGGGGCGCGgggccc                                                           >  1:73398/1‑97 (MQ=14)
                                           acaTTGCCACCGCGACCGCAGAGGGCCCAGAGGCCCTCGGCC.TGCCCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTcc                                                          <  2:3810791/101‑1 (MQ=14)
                                            cttcgccccgGTGACCGCGGGGGCCCCAGAGCCCCTCCGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTccc                                                         <  1:3612197/95‑1 (MQ=14)
                                            caTTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTAGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGGATTCACG.CCCCCGGCGCGGGGCGCAGGcagccc                                                         >  2:1967727/1‑96 (MQ=14)
                                              ttGGCCCCGGGCCCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCcgc                                                       <  2:2599868/101‑1 (MQ=255)
                                                gcccccGTGCCCGCGGAGGGCCCAGAGGCCCTCGCCT.TGCCCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCcgcgc                                                     <  1:2470706/99‑1 (MQ=17)
                                                 gCCCCGTCCCCGCCGAGGGCCCAGGGCCCCTCGGCC.GGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCcgcgcg                                                    <  1:2331821/101‑1 (MQ=17)
                                                    ccGTGCCCGCCGAGGGCCCAGAGGCCCTCGCCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCtt                                                 <  1:3449785/101‑1 (MQ=255)
                                                    ccGTGCCCCCCGAGGCCCCAGAGCCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCtt                                                 <  2:4203554/101‑1 (MQ=18)
                                                     tgTGACCGCCGAGGGCCCAAAGGCACTCGGCC.TGCGCCCGCCCCGCCATCGGCGGGGGGCGCCGCG.CCGCCCGCGGGGGGGGCAGGCCCCCcgtttggctg                                                >  2:3970896/2‑93 (MQ=2)
                                                      gggcccgccgAGGGCCCGGGGGCCCTCGGCC.GGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTgg                                               <  1:3500583/97‑1 (MQ=17)
                                                      gTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCCTTCCCG.CCCCCGGGGCGGGGCGCCGGGCCCCcgcgccccttg                                               >  2:2073508/1‑95 (MQ=14)
                                                        ggccggcgAGGGCCCAGGGGCCCGCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTgggg                                             <  1:2012/99‑1 (MQ=255)
                                                        gACCGCCGGGGGCCCAGAGCCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTgggg                                             <  2:2375524/101‑1 (MQ=255)
                                                        gACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCCGGGGGGGGCGCAGGGCTCCCCCGCGCCTtggg                                             >  2:2900530/1‑101 (MQ=14)
                                                         cccgcggAGGGCCAGGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGa                                            <  2:632915/100‑1 (MQ=255)
                                                           cgccgAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGCCTTCGCG.CCGCCGGGGGGGGGCGCAGGGCCCCCCCGCGCCTGGGGGgg                                          >  2:1582202/1‑101 (MQ=14)
                                                            gccgAGGGCCCAGAGGCCCGCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGc                                         <  2:736958/101‑1 (MQ=255)
                                                            gccgAGGCCCCAGGGCCCCGCGGCC.TGCACCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGAAGGCCTCCCGCGCGCTTGGGGAGGc                                         <  2:127945/101‑1 (MQ=18)
                                                              cgAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCCCCTTCGGG.CCGCCGGGGCCGGGGGCGGGCCTCCCCCGCGCCTTGGGAggggg                                       >  2:4036119/1‑98 (MQ=14)
                                                               gggggCCCAAAGGCCCTCGGCC.TGCGCCCGCCCCGCCATCGGCGGGCGGCTTCGCG.CCCCCCGGGGGGGGGGCGGGGCGCCCTCGGGCTTtgggggagggg                                      >  1:4019022/3‑94 (MQ=2)
                                                               gAGGCCCCGGGGCCCCCCGCCC.TGCCCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGAt                                      <  2:3635079/101‑1 (MQ=18)
                                                                ggggCCCGGGGCCCCCGGGCC.GGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATc                                     <  1:752075/100‑1 (MQ=18)
                                                                aGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCCGGGCGGGGCGCCGGCCCCCCCCCCCCCTGGGGGgggggtc                                     >  1:3391937/1‑96 (MQ=11)
                                                                 gggCCCAAAGGCCCTCGGCC.TGCGCCCGCCCCGCCATCGGCGGGGGGAATCGCGCCCCCCGGGGCGGG.GGGAGGCCCCCCGCGTGGTTtgggggacggtat                                    >  1:3071430/1‑92 (MQ=2)
                                                                  ggCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGCCTTCGCG.CCGCCGGGGCGGGGCGCCGGCCCCCCCCGCGCCTGGGGGGGGGGTCtt                                   >  1:3246595/1‑101 (MQ=14)
                                                                  gccccAGGGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCtt                                   <  2:3252794/99‑1 (MQ=255)
                                                                  gccccAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCtt                                   <  2:2945820/99‑1 (MQ=255)
                                                                   gCCCGGAGGCCCGGCGCG.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTg                                  <  1:2593223/101‑1 (MQ=21)
                                                                    cccAGAGCCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGt                                 <  1:211293/101‑1 (MQ=255)
                                                                         gggCCCTCCGCCTTGGGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCAcc                             <  2:4153337/100‑1 (MQ=21)
                                                                         gagcctccGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCg                            <  2:1030032/94‑1 (MQ=255)
                                                                         agGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGCATTCGCG.CCGCCGGGGCGGGGCGCCGGCCTCCCCCGCGCTTGGGGGGGGGGTCTTGTCACCg                            >  1:1748381/1‑101 (MQ=17)
                                                                         agGCCCACCGCC.GGCCCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGCGCGGGACTCAGGCCTCCCGCGCGCTTGGGGAGGTGATCTTGTCACCg                            <  1:2073646/101‑1 (MQ=21)
                                                                          ggCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAAGCCTCCCCCGCGCCTGGGGGGGGGGTCTTGTCAcccg                           >  1:1482487/1‑99 (MQ=17)
                                                                            cccTCGGCC.TGCCCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCt                         <  2:3710248/101‑1 (MQ=255)
                                                                             ccccGGCC.TGCCCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCtt                        <  2:3186771/98‑1 (MQ=255)
                                                                             ccTCGCCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCtt                        <  1:1640468/101‑1 (MQ=255)
                                                                              cgcGGCC.GGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTg                       <  1:1585573/99‑1 (MQ=255)
                                                                               gcGGCC.GGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTgg                      <  2:3899725/100‑1 (MQ=255)
                                                                                cGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCCGGGGGGGACGCAGGCCTCCCCCGCGCTTGGGGAGGCGAGCTTGTCCCCGGCtttgg                     >  2:1188807/1‑98 (MQ=18)
                                                                                 ggCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGGCGCAGGCCTCCCGCGCGCTTTGGGGGGCGGTCTTGTCACCGGCtttggc                    >  1:3372310/1‑97 (MQ=18)
                                                                                  gCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGGGGGACGCAGGCCTCCCGCGCGCTTGGGGGGGCGATCTTGTCACCGGCTTTGTcg                   >  1:1271274/1‑101 (MQ=255)
                                                                                  gCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTcg                   <  1:1389270/101‑1 (MQ=255)
                                                                                  gCC.GGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTcg                   <  2:1142126/101‑1 (MQ=255)
                                                                                   gcttgtgcccgcccCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGTTcg                   <  2:922023/95‑1 (MQ=255)
                                                                                      tGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGGGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGGTCTTGTCACCCGCtttggtgcgg                >  2:169447/1‑93 (MQ=18)
                                                                                        cgcccgccCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGAcg              <  1:325612/101‑1 (MQ=255)
                                                                                         gcccgcccCGCCCATGGCGGGCGCATTCACG.CCCCCGGGGCGGGGCGACGGGCTCCCGGGCGCCTGGGGAGGGGGTCTTGTCACCGGCATTGTcgggtgcg             >  2:2072586/1‑95 (MQ=12)
                                                                                          cccgccccgcCCATGGCGGGGGCATTCGCG.CCGCCGGGGCGGGGCGCAGGGCTCCCGCGCGCTTGGGGAGGCGATCTTGTCCCCGGCTTTGTTGCGGcggg            >  2:3678581/1‑101 (MQ=18)
                                                                                          cccgccccgcCCATGGCGGGCGCATTCGCG.CCGCCGGGGGGGGGCGCAGGCCTCCCCCGCGCTTGGGGGGGGGGTCTTGTCCCCGGCTTTGGCGCGGcggg            >  2:3575229/1‑101 (MQ=17)
                                                                                           ccgccccgccCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGAcgggc           <  2:1745905/101‑1 (MQ=255)
                                                                                            cgccccgcccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGAcgggcg          <  1:3178797/101‑1 (MQ=255)
                                                                                               cccgcccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCgggg       <  2:2238880/101‑1 (MQ=255)
                                                                                                ccgcccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCGGGGa      <  2:1460733/101‑1 (MQ=255)
                                                                                                ccgcccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCGGGGa      <  2:729349/101‑1 (MQ=255)
                                                                                                ccgcccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGGCGCGCAGGGGggggg      >  2:3852951/1‑100 (MQ=25)
                                                                                                 cgcccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCGGGGaa     <  2:3604678/101‑1 (MQ=255)
                                                                                                   cccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCGGGGAAAg   <  1:2317189/101‑1 (MQ=255)
                                                                                                    ccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCCTGGGGGGGCGATCTTGTCACCGGCTTGGGTGCGGCCGGGGGGGGAAgg  >  2:2551825/1‑101 (MQ=25)
                                                                                                     |                                                                                                    
CGATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCGGGGAAAGG  >  NC_014034/1612326‑1612525

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG