Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,612,427 A→T 3.1% intergenic (+105/‑256) purN → / → rnd2 phosphoribosylglycinamide formyltransferase/ribonuclease D

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,612,4270A→T 3.1% 6.7 ‑4.4 ‑0.1160intergenic (+105/‑256)purN/rnd2phosphoribosylglycinamide formyltransferase/ribonuclease D
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (63/92):  total (68/92)
Fisher's exact test strand distribution p-value = 1.27e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.12e-01

Only 100 of 179 total aligned reads displayed.

GATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCGGGGAAAGG  >  NC_014034/1612327‑1612525
                                                                                                     |                                                                                                   
 acccGCTGATCGGACCGGGCCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCAt                                                                                                    <  2:45843/99‑1 (MQ=255)
  tCCGCTGATCTGCCCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATg                                                                                                   <  2:1859333/101‑1 (MQ=255)
  tCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATg                                                                                                   >  2:3118042/1‑101 (MQ=255)
   ccGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATgg                                                                                                  >  1:1342368/1‑101 (MQ=255)
   ccGCTGACCTGACCGAGGCCACCGCCGGGGTCCCGCGACACATCGGCCCCGTGACCGCCGAGGGCCCAGAGCCCCTCGGCC.TGCGCCCGCCCCGCCCATgg                                                                                                  <  1:402836/101‑1 (MQ=18)
     gCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATggcg                                                                                                >  1:2792029/1‑101 (MQ=255)
      cTGATCTGCCCGGGCCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATggcgg                                                                                               <  1:892415/101‑1 (MQ=255)
          tcggcgcGAGGCCCGCGCCGAGCTCCCGCGACACATGGGCCCCGGGACCGCGGGGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGgcgc                                                                                           <  1:1278985/95‑1 (MQ=14)
           cTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCa                                                                                          <  2:599074/101‑1 (MQ=255)
            tGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGTCC.TGCCCCCGCCCCGCCCACggcggggggag                                                                                         >  2:494189/1‑96 (MQ=17)
            tGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGCac                                                                                         >  1:4130365/1‑100 (MQ=255)
            tGACCGAGGCCATCGCCGAGGACACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGCAt                                                                                         >  1:2405727/1‑101 (MQ=255)
              aCCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATggcggggggcctt                                                                                       >  1:2252578/1‑94 (MQ=255)
                 gAGCCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTcgcg                                                                                    <  2:2123189/101‑1 (MQ=255)
                  aGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGGATTCgcg.c                                                                                  >  2:3671566/1‑101 (MQ=255)
                  aGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TACGCCCGCCCCGCCCATGGCGGgggcgctttcgc                                                                                   >  1:3604931/1‑93 (MQ=17)
                   ggCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGCCCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGcg.cc                                                                                 <  1:3928273/101‑1 (MQ=255)
                   ggCCAGCGCCGAGGTCCCGCGACACATGGGCCCCGTGCCCGCCGAGGGCCCAGAGCCCCTCGCCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGcg.cc                                                                                 <  1:4159823/101‑1 (MQ=17)
                    gCCATCGCCGAGGTCACGCGACACTTGGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGcg.ccg                                                                                <  2:2842893/101‑1 (MQ=255)
                     ccATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGCATGTGcg.cccc                                                                               >  1:160558/1‑99 (MQ=255)
                     ccATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCAGTCGcc.ccgc                                                                               >  2:4123234/1‑101 (MQ=255)
                      cATCGCCGAGGCCCCGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCg.ccgcc                                                                              <  2:3344702/101‑1 (MQ=255)
                      atctcccGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCg.ccgcc                                                                              <  2:2490929/96‑1 (MQ=255)
                         cGCCGGGGTCACGCGACACATTGGCCCCGTGCCCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCggg                                                                           <  1:2175728/101‑1 (MQ=255)
                          gCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGTGCGTTTGCGCCCGCCggg                                                                           >  2:981814/1‑101 (MQ=17)
                           ccGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCCATCCCg.cccccccggc                                                                         >  2:2650930/1‑96 (MQ=17)
                                 ccccgcgCCACATTGGCCCCGGGCCCGCCGAGGGCCCGGAGCCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACg                                                                   <  1:1131099/98‑1 (MQ=17)
                                  cACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGGCTTCCCG.CCGCCGGGGGggggggc                                                                  >  1:1578646/1‑97 (MQ=17)
                                  cACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCCGGGGggggggc                                                                  >  2:850555/1‑97 (MQ=255)
                                   aCGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCCTTCGCG.CCCCCCGGGGggggggcc                                                                 >  1:1310750/1‑96 (MQ=17)
                                      cgACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGCATTCGCG.CCGCCGGGGGGGGGCGCAGGc                                                              >  1:1746024/1‑101 (MQ=255)
                                      cgACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCCTTCGCG.CCGCCCGGGCGGGGGGCAGGc                                                              >  1:3887733/1‑101 (MQ=17)
                                      cgACACATTGCCCCCGTGCCCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGc                                                              <  2:2791810/101‑1 (MQ=255)
                                      caacacATGGGCCCCGTGCCCCCCGAGGGCCCGGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGc                                                              <  2:1927544/99‑1 (MQ=17)
                                       gACACATTGGCCCCGTGACCGCCGAGGGCCTCTGGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCCGGGCGGGGCGCCgggc                                                             >  2:1348293/1‑99 (MQ=14)
                                         cacaTTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCCTTCGCG.CCCCCCGGGCGGGGCGCGgggccc                                                           >  1:73398/1‑97 (MQ=14)
                                          acaTTGCCACCGCGACCGCAGAGGGCCCAGAGGCCCTCGGCC.TGCCCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTcc                                                          <  2:3810791/101‑1 (MQ=14)
                                           caTTGGCCCCGTGCCCGCCGAGGGCCCAGAGCCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTccc                                                         <  2:3522752/101‑1 (MQ=255)
                                           caTTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCCTTCGCG.CCGCCGGGGGGGGGGGCAGGCCCccc                                                         >  1:34323/1‑101 (MQ=14)
                                           caTTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTAGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGGATTCACG.CCCCCGGCGCGGGGCGCAGGcagccc                                                         >  2:1967727/1‑96 (MQ=14)
                                                gCCCCGTCCCCGCCGAGGGCCCAGGGCCCCTCGGCC.GGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCcgcgcg                                                    <  1:2331821/101‑1 (MQ=17)
                                                   ccGTGCCCGCCGGGGGCCCGGGGGCCCTCGGCC.GGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCtt                                                 <  1:95538/101‑1 (MQ=18)
                                                   ccGTGCCCCCCGAGGCCCCAGAGCCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCtt                                                 <  2:4203554/101‑1 (MQ=18)
                                                    tgTGACCGCCGAGGGCCCAAAGGCACTCGGCC.TGCGCCCGCCCCGCCATCGGCGGGGGGCGCCGCG.CCGCCCGCGGGGGGGGCAGGCCCCCcgtttggctg                                                >  2:3970896/2‑93 (MQ=2)
                                                    cGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGCATTCGCG.CCCCCCGGGCGGGGGCCAGGCCCCCcccgcccctg                                                >  1:3681279/1‑96 (MQ=11)
                                                       ggccggcgAGGGCCCAGGGGCCCGCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTgggg                                             <  1:2012/99‑1 (MQ=255)
                                                       gACCGCCGGGGGCCCAGAGCCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTgggg                                             <  2:2375524/101‑1 (MQ=255)
                                                        cccgcggAGGGCCAGGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGa                                            <  2:632915/100‑1 (MQ=255)
                                                          cgcggggggCCCAGGGGCCCTCGGCC.GGCCCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAgg                                          <  1:3216299/95‑1 (MQ=18)
                                                          cgccgAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGCCTTCGCG.CCGCCGGGGGGGGGCGCAGGGCCCCCCCGCGCCTGGGGGgg                                          >  2:1582202/1‑101 (MQ=14)
                                                           gccgAGGGCCCGGGGGCCCTCGGCC.GGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGc                                         <  2:2646132/101‑1 (MQ=255)
                                                           gccgAGGGCCCAGAGGCCCGCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGc                                         <  2:736958/101‑1 (MQ=255)
                                                             cgAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCCCCTTCGGG.CCGCCGGGGCCGGGGGCGGGCCTCCCCCGCGCCTTGGGAggggg                                       >  2:4036119/1‑98 (MQ=14)
                                                              gAGGCCCCGGGGCCCCCCGCCC.TGCCCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGAt                                      <  2:3635079/101‑1 (MQ=18)
                                                               ggggCCCGGGGCCCCCGGGCC.GGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATc                                     <  1:752075/100‑1 (MQ=18)
                                                               aGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCCTTTGCG.CCCCCCGGGGGGGGCGGGGGGCCCCCCCCCGCCTgggggtggggcc                                     >  1:668895/1‑93 (MQ=2)
                                                               aGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCCGGGCGGGGCGCCGGCCCCCCCCCCCCCTGGGGGgggggtc                                     >  1:3391937/1‑96 (MQ=11)
                                                                gggCCCAAAGGCCCTCGGCC.TGCGCCCGCCCCGCCATCGGCGGGGGGAATCGCGCCCCCCGGGGCGGG.GGGAGGCCCCCCGCGTGGTTtgggggacggtat                                    >  1:3071430/1‑92 (MQ=2)
                                                                gggCCCAAAGGCCCTCGGCC.TGCGCCCGCCCCGCCATCGGCGGGCGCATTCCGC.CCGCCCGGGCGGGGGGCGGGGCCCCCGCCGGCTTggggggacggggt                                    >  2:1378165/1‑92 (MQ=2)
                                                                 gccccAGGGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCtt                                   <  2:3252794/99‑1 (MQ=255)
                                                                  gCCCGGAGGCCCGGCGCG.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTg                                  <  1:2593223/101‑1 (MQ=21)
                                                                      ggggccccTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCAc                              <  2:2931852/96‑1 (MQ=255)
                                                                        gggCCCTCCGCCTTGGGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCAcc                             <  2:4153337/100‑1 (MQ=21)
                                                                        gagcctccGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCg                            <  2:1030032/94‑1 (MQ=255)
                                                                        agGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGCATTCGCG.CCGCCGGGGCGGGGCGCCGGCCTCCCCCGCGCTTGGGGGGGGGGTCTTGTCACCg                            >  1:1748381/1‑101 (MQ=17)
                                                                        agGCCCACCGCC.GGCCCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGCGCGGGACTCAGGCCTCCCGCGCGCTTGGGGAGGTGATCTTGTCACCg                            <  1:2073646/101‑1 (MQ=21)
                                                                         ggCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCCATAGCG.CCCCCGGGGAGGGGAGCAGGGCTCCNCCGCGCCTGGGGGGGGGGGCTTGTCACCgg                           >  1:382629/1‑101 (MQ=9)
                                                                         ggCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAAGCCTCCCCCGCGCCTGGGGGGGGGGTCTTGTCAcccg                           >  1:1482487/1‑99 (MQ=17)
                                                                           cccTCGGCC.TGCCCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCt                         <  2:3710248/101‑1 (MQ=255)
                                                                           cccCCGGCC.GGCCCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCt                         <  1:3118273/101‑1 (MQ=255)
                                                                            ccccGGCC.TGCCCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCtt                        <  2:3186771/98‑1 (MQ=255)
                                                                             cgcGGCC.GGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTg                       <  1:1585573/99‑1 (MQ=255)
                                                                              gcGGCC.GGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTgg                      <  2:3899725/100‑1 (MQ=255)
                                                                               cGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCCGGGGGGGACGCAGGCCTCCCCCGCGCTTGGGGAGGCGAGCTTGTCCCCGGCtttgg                     >  2:1188807/1‑98 (MQ=18)
                                                                                ggCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGGCGCAGGCCTCCCGCGCGCTTTGGGGGGCGGTCTTGTCACCGGCtttggc                    >  1:3372310/1‑97 (MQ=18)
                                                                                 gCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGGGGGACGCAGGCCTCCCGCGCGCTTGGGGGGGCGATCTTGTCACCGGCTTTGTcg                   >  1:1271274/1‑101 (MQ=255)
                                                                                 gCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTcg                   <  1:1389270/101‑1 (MQ=255)
                                                                                 gCC.GGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTcg                   <  2:1142126/101‑1 (MQ=255)
                                                                                  gcttgtgcccgcccCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGTTcg                   <  2:922023/95‑1 (MQ=255)
                                                                                   c.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGGTCTTGTCACCGGCTTTGTcgcg                 >  2:3064962/1‑101 (MQ=255)
                                                                                   c.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTcgcg                 <  1:1358769/101‑1 (MQ=255)
                                                                                   c.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTcgcg                 <  1:2081612/101‑1 (MQ=255)
                                                                                     tGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGGGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGGTCTTGTCACCCGCtttggtgcgg                >  2:169447/1‑93 (MQ=18)
                                                                                       cgcccgccCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGAcg              <  1:325612/101‑1 (MQ=255)
                                                                                        gcccgcccCGCCCATGGCGGGCGCATTCACG.CCCCCGGGGCGGGGCGACGGGCTCCCGGGCGCCTGGGGAGGGGGTCTTGTCACCGGCATTGTcgggtgcg             >  2:2072586/1‑95 (MQ=12)
                                                                                         cccgccccgcCCATGGCGGGGGCATTCGCG.CCGCCGGGGCGGGGCGCAGGGCTCCCGCGCGCTTGGGGAGGCGATCTTGTCCCCGGCTTTGTTGCGGcggg            >  2:3678581/1‑101 (MQ=18)
                                                                                         cccgccccgcCCATGGCGGGCGCATTCGCG.CCGCCGGGGGGGGGCGCAGGCCTCCCCCGCGCTTGGGGGGGGGGTCTTGTCCCCGGCTTTGGCGCGGcggg            >  2:3575229/1‑101 (MQ=17)
                                                                                         cccgccccgcCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGAcggg            <  2:10133/101‑1 (MQ=255)
                                                                                           cgccccgcccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGAcgggcg          <  1:626841/101‑1 (MQ=255)
                                                                                              cccgcccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCgggg       <  2:2238880/101‑1 (MQ=255)
                                                                                               ccgcccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCGGGGa      <  2:729349/101‑1 (MQ=255)
                                                                                               ccgcccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCGGGGa      <  2:1460733/101‑1 (MQ=255)
                                                                                               ccgcccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGGCGCGCAGGGGggggg      >  2:3852951/1‑100 (MQ=25)
                                                                                                cgcccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCGGGGaa     <  2:3604678/101‑1 (MQ=255)
                                                                                                cgcccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCGGGGaa     <  2:1747447/101‑1 (MQ=255)
                                                                                                 gcccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCGGGGaaa    <  1:4123495/101‑1 (MQ=255)
                                                                                                 gcccATGCCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCCGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCGGGGaaa    <  1:3452523/101‑1 (MQ=255)
                                                                                                  cccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCGGGGAAAg   <  1:4014289/101‑1 (MQ=255)
                                                                                                  cccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCGGGGAAAg   <  1:2317189/101‑1 (MQ=255)
                                                                                                   ccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCCTGGGGGGGCGATCTTGTCACCGGCTTGGGTGCGGCCGGGGGGGGAAgg  >  2:2551825/1‑101 (MQ=25)
                                                                                                     |                                                                                                   
GATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCGACGGGCGGGGAAAGG  >  NC_014034/1612327‑1612525

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG