Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,612,428 T→C 3.2% intergenic (+106/‑255) purN → / → rnd2 phosphoribosylglycinamide formyltransferase/ribonuclease D

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,612,4280T→C 3.2% 4.8 ‑4.4 ‑1.7158intergenic (+106/‑255)purN/rnd2phosphoribosylglycinamide formyltransferase/ribonuclease D
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (62/91):  total (67/91)
Fisher's exact test strand distribution p-value = 1.25e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.82e-02

Only 100 of 179 total aligned reads displayed.

ATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCG.ACGGGCGGGGAAAGGCGA  >  NC_014034/1612328‑1612528
                                                                                                     |                                                                                                      
acccGCTGATCGGACCGGGCCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCAt                                                                                                        <  2:45843/99‑1 (MQ=255)
 tCCGCTGATCTGCCCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATg                                                                                                       <  2:1859333/101‑1 (MQ=255)
  ccGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATgg                                                                                                      >  1:1342368/1‑101 (MQ=255)
    gCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATggcg                                                                                                    >  1:2792029/1‑101 (MQ=255)
     cTGATCTGCCCGGGCCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATggcgg                                                                                                   <  1:892415/101‑1 (MQ=255)
      tGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATggcggg                                                                                                  >  1:4222839/1‑101 (MQ=255)
         tcggcgcGAGGCCCGCGCCGAGCTCCCGCGACACATGGGCCCCGGGACCGCGGGGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGgcgc                                                                                               <  1:1278985/95‑1 (MQ=14)
          cTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCa                                                                                              <  2:599074/101‑1 (MQ=255)
           tGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGTCC.TGCCCCCGCCCCGCCCACggcggggggag                                                                                             >  2:494189/1‑96 (MQ=17)
           tGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGgcgcgt                                                                                             >  1:3341656/1‑99 (MQ=255)
           tGACCGAGGCCATCGCCGAGGACACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGCAt                                                                                             >  1:2405727/1‑101 (MQ=255)
             aCCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATggcggggggcgtt                                                                                           >  2:325567/1‑94 (MQ=255)
             aCCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATggcggggggcctt                                                                                           >  1:2252578/1‑94 (MQ=255)
               cGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGCACTcgc                                                                                         >  1:4153599/1‑101 (MQ=255)
               cGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCCTTccc                                                                                         >  2:34883/1‑99 (MQ=255)
                gAGCCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTcgcg                                                                                        <  2:2123189/101‑1 (MQ=255)
                 aGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGGATTCgcg.c                                                                                      >  2:3671566/1‑101 (MQ=255)
                  ggCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCCCTCGcg.cc                                                                                     >  1:4075187/1‑101 (MQ=255)
                    ccATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGCATGTGcg.cccc                                                                                   >  1:160558/1‑99 (MQ=255)
                    ccATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCAGTCGcc.ccgc                                                                                   >  2:4123234/1‑101 (MQ=255)
                     caccGCGGAGGTCCCGCGACACATTGGCCCCGTGACCGCCGGGGGCCCAGGGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCg.ccgcc                                                                                  <  1:3931526/98‑1 (MQ=17)
                      cccGCCGAGGTCCCGCGACACATGGGCCCCGTGCCCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.ccgccg                                                                                 <  2:3127083/99‑1 (MQ=255)
                        cGCCGGGGTCACGCGACACATTGGCCCCGTGCCCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCggg                                                                               <  1:2175728/101‑1 (MQ=255)
                         gCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGTGCGTTTGCGCCCGCCggg                                                                               >  2:981814/1‑101 (MQ=17)
                             gggccgcgcgACACATTGGCCCCGTGCCCGCGGGGGCCCCGGAGGCCCCCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCggg                                                                          <  1:3908499/95‑1 (MQ=17)
                                ccccgcgCCACATTGGCCCCGGGCCCGCCGAGGGCCCGGAGCCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACg                                                                       <  1:1131099/98‑1 (MQ=17)
                                 cACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGGCTTCCCG.CCGCCGGGGGggggggc                                                                      >  1:1578646/1‑97 (MQ=17)
                                 cACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCCGGGGggggggc                                                                      >  2:850555/1‑97 (MQ=255)
                                  aCGCGACACATTGGCCCCGTGCCCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCa                                                                     <  2:2813280/101‑1 (MQ=255)
                                  aCGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCCTTCGCG.CCCCCCGGGGggggggcc                                                                     >  1:1310750/1‑96 (MQ=17)
                                     cgACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGCATTCGCG.CCGCCGGGGGGGGGCGCAGGc                                                                  >  1:1746024/1‑101 (MQ=255)
                                     caacacATGGGCCCCGTGCCCCCCGAGGGCCCGGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGc                                                                  <  2:1927544/99‑1 (MQ=17)
                                      gACACATTGGCCCCGTGACCGCCGAGGGCCTCTGGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCCGGGCGGGGCGCCgggc                                                                 >  2:1348293/1‑99 (MQ=14)
                                       acacATTGGCCCCGTGCCCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCt                                                                <  1:3729844/101‑1 (MQ=255)
                                        cacaTTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCCTTCGCG.CCCCCCGGGCGGGGCGCGgggccc                                                               >  1:73398/1‑97 (MQ=14)
                                         acaTTGCCACCGCGACCGCAGAGGGCCCAGAGGCCCTCGGCC.TGCCCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTcc                                                              <  2:3810791/101‑1 (MQ=14)
                                          cttcgccccgGTGACCGCGGGGGCCCCAGAGCCCCTCCGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTccc                                                             <  1:3612197/95‑1 (MQ=14)
                                          caTTGGCCCCGTGCCCGCCGAGGGCCCAGAGCCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTccc                                                             <  2:3522752/101‑1 (MQ=255)
                                          caTTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATAGCGGGGGCCGGCGCG.TCGCCGCGGGGGGAGGCGAGCCGccc                                                             >  2:2274476/1‑101 (MQ=14)
                                          caTTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTAGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGGATTCACG.CCCCCGGCGCGGGGCGCAGGcagccc                                                             >  2:1967727/1‑96 (MQ=14)
                                            ttGGCCCCGGGCCCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCcgc                                                           <  2:2599868/101‑1 (MQ=255)
                                               gCCCCGTCCCCGCCGAGGGCCCAGGGCCCCTCGGCC.GGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCcgcgcg                                                        <  1:2331821/101‑1 (MQ=17)
                                                  cccggACCACGGAGGCCCCAGAGTCCCTGGGCT.TTCCCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCtt                                                     <  2:2761333/97‑1 (MQ=17)
                                                  ccGTGCCCGCCGGGGGCCCGGGGGCCCTCGGCC.GGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCtt                                                     <  1:95538/101‑1 (MQ=18)
                                                  ccGTGCCCGCCGAGGGCCCAGAGGCCCTCGCCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCtt                                                     <  1:3449785/101‑1 (MQ=255)
                                                  ccGTGCCCCCCGAGGCCCCAGAGCCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCtt                                                     <  2:4203554/101‑1 (MQ=18)
                                                   tgTGACCGCCGAGGGCCCAAAGGCACTCGGCC.TGCGCCCGCCCCGCCATCGGCGGGGGGCGCCGCG.CCGCCCGCGGGGGGGGCAGGCCCCCcgtttggctg                                                    >  2:3970896/2‑93 (MQ=2)
                                                    gggcccgccgAGGGCCCGGGGGCCCTCGGCC.GGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTgg                                                   <  1:3500583/97‑1 (MQ=17)
                                                    gTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCCGGGGGGGGCGCAGGCCTCCCCCGCGCCTgg                                                   >  2:3194554/1‑101 (MQ=14)
                                                      ggccggcgAGGGCCCAGGGGCCCGCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTgggg                                                 <  1:2012/99‑1 (MQ=255)
                                                      gACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCCGGGGGGGGCGCAGGGCTCCCCCGCGCCTtggg                                                 >  2:2900530/1‑101 (MQ=14)
                                                       cccgcggAGGGCCAGGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGa                                                <  2:632915/100‑1 (MQ=255)
                                                         cgccgAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGCCTTCGCG.CCGCCGGGGGGGGGCGCAGGGCCCCCCCGCGCCTGGGGGgg                                              >  2:1582202/1‑101 (MQ=14)
                                                          gccgAGGGCCCGGGGGCCCTCGGCC.GGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGc                                             <  2:2646132/101‑1 (MQ=255)
                                                          gccgAGGGCCCAGAGGCCCGCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGc                                             <  2:736958/101‑1 (MQ=255)
                                                            cgAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCCCCTTCGGG.CCGCCGGGGCCGGGGGCGGGCCTCCCCCGCGCCTTGGGAggggg                                           >  2:4036119/1‑98 (MQ=14)
                                                             gggggCCCAAAGGCCCTCGGCC.TGCGCCCGCCCCGCCATCGGCGGGCGGCTTCGCG.CCCCCCGGGGGGGGGGCGGGGCGCCCTCGGGCTTtgggggagggg                                          >  1:4019022/3‑94 (MQ=2)
                                                             gAGGCCCCGGGGCCCCCCGCCC.TGCCCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGAt                                          <  2:3635079/101‑1 (MQ=18)
                                                              ggggCCCGGGGCCCCCGGGCC.GGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATc                                         <  1:752075/100‑1 (MQ=18)
                                                              aGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCCTTTGCG.CCCCCCGGGGGGGGCGGGGGGCCCCCCCCCGCCTgggggtggggcc                                         >  1:668895/1‑93 (MQ=2)
                                                              aGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCCGGGCGGGGCGCCGGCCCCCCCCCCCCCTGGGGGgggggtc                                         >  1:3391937/1‑96 (MQ=11)
                                                               gggCCCAAAGGCCCTCGGCC.TGCGCCCGCCCCGCCATCGGCGGGGGGAATCGCGCCCCCCGGGGCGGG.GGGAGGCCCCCCGCGTGGTTtgggggacggtat                                        >  1:3071430/1‑92 (MQ=2)
                                                               gggCCCAAAGGCCCTCGGCC.TGCGCCCGCCCCGCCATCGGCGGGCGCATTCCGC.CCGCCCGGGCGGGGGGCGGGGCCCCCGCCGGCTTggggggacggggt                                        >  2:1378165/1‑92 (MQ=2)
                                                                ggCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGCCTTCGCG.CCGCCGGGGCGGGGCGCCGGCCCCCCCCGCGCCTGGGGGGGGGGTCtt                                       >  1:3246595/1‑101 (MQ=14)
                                                                ggCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGGCGCAGGCCTCCCCCCCGCTTTGGGGGGGGGTCtt                                       >  2:2412089/1‑101 (MQ=14)
                                                                gccccAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCtt                                       <  2:2945820/99‑1 (MQ=255)
                                                                 gCCCGGAGGCCCGGCGCG.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTg                                      <  1:2593223/101‑1 (MQ=21)
                                                                 gCCCGGAGGCCCCCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTg                                      <  1:3372755/101‑1 (MQ=255)
                                                                       gggCCCTCCGCCTTGGGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCAcc                                 <  2:4153337/100‑1 (MQ=21)
                                                                       gagcctccGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCg                                <  2:1030032/94‑1 (MQ=255)
                                                                       agGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGGGCATTCGCG.CCGCCGGGGCGGGGCGCCGGCCTCCCCCGCGCTTGGGGGGGGGGTCTTGTCACCg                                >  1:1748381/1‑101 (MQ=17)
                                                                       agGCCCACCGCC.GGCCCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGCGCGGGACTCAGGCCTCCCGCGCGCTTGGGGAGGTGATCTTGTCACCg                                <  1:2073646/101‑1 (MQ=21)
                                                                        ggCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCCATAGCG.CCCCCGGGGAGGGGAGCAGGGCTCCNCCGCGCCTGGGGGGGGGGGCTTGTCACCgg                               >  1:382629/1‑101 (MQ=9)
                                                                        ggCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAAGCCTCCCCCGCGCCTGGGGGGGGGGTCTTGTCAcccg                               >  1:1482487/1‑99 (MQ=17)
                                                                          cccTCGGCC.TGCCCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCt                             <  2:3710248/101‑1 (MQ=255)
                                                                            cgcGGCC.GGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTg                           <  1:1585573/99‑1 (MQ=255)
                                                                             gcGGCC.GGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTgg                          <  2:3899725/100‑1 (MQ=255)
                                                                              cGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCCGGGGGGGACGCAGGCCTCCCCCGCGCTTGGGGAGGCGAGCTTGTCCCCGGCtttgg                         >  2:1188807/1‑98 (MQ=18)
                                                                                gCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGGGGGACGCAGGCCTCCCGCGCGCTTGGGGGGGCGATCTTGTCACCGGCTTTGTcg                       >  1:1271274/1‑101 (MQ=255)
                                                                                gCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTcg                       <  2:3586704/101‑1 (MQ=255)
                                                                                gCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTcg                       <  1:1389270/101‑1 (MQ=255)
                                                                                gCC.GGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTcg                       <  2:1142126/101‑1 (MQ=255)
                                                                                 gcttgtgcccgcccCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGTTcg                       <  2:922023/95‑1 (MQ=255)
                                                                                  c.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGGTCTTGTCACCGGCTTTGTcgcg                     >  2:3064962/1‑101 (MQ=255)
                                                                                  c.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTcgcg                     <  1:1358769/101‑1 (MQ=255)
                                                                                  c.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTcgcg                     <  1:2081612/101‑1 (MQ=255)
                                                                                    tGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGGGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGGTCTTGTCACCCGCtttggtgcgg                    >  2:169447/1‑93 (MQ=18)
                                                                                       gcccgcccCGCCCATGGCGGGCGCATTCACG.CCCCCGGGGCGGGGCGACGGGCTCCCGGGCGCCTGGGGAGGGGGTCTTGTCACCGGCATTGTcgggtgcg                 >  2:2072586/1‑95 (MQ=12)
                                                                                        cccgccccgcCCATGGCGGGGGCATTCGCG.CCGCCGGGGCGGGGCGCAGGGCTCCCGCGCGCTTGGGGAGGCGATCTTGTCCCCGGCTTTGTTGCG.Gcggg               >  2:3678581/1‑101 (MQ=18)
                                                                                        cccgccccgcCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCG.Acggg               <  2:10133/101‑1 (MQ=255)
                                                                                          cgccccgcccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCG.Acgggcg             <  1:3178797/101‑1 (MQ=255)
                                                                                              ccgcccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCG.ACGGGCGGGGa         <  2:1460733/101‑1 (MQ=255)
                                                                                              ccgcccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCG.ACGGGCGGGGa         <  2:729349/101‑1 (MQ=255)
                                                                                              ccgcccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGGCGCG.CAGGGGggggg         >  2:3852951/1‑100 (MQ=25)
                                                                                               cgcccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCG.ACGGGCGGGGaa        <  2:1747447/101‑1 (MQ=255)
                                                                                                gcccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCG.ACGGGCGGGGaaa       <  1:4123495/101‑1 (MQ=255)
                                                                                                 cccATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCG.ACGGGCGGGGAAAg      <  1:2317189/101‑1 (MQ=255)
                                                                                                   cATGGCGGGCGCATTTGCG.CCGCCGGGGGGGGGCGCAGGCCACCCGCCCGCTTGGGGGGGGGATCTTGTCACCGGCTTTGGTGCGCACGGGGgggggaggg     >  2:2545377/1‑96 (MQ=16)
                                                                                                     tGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCG.ACGGGCGGGGAAAGGCGa  <  2:2180560/101‑1 (MQ=255)
                                                                                                     tGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGGCGCG.ACGGGGGGGGGAAGGGGa  >  1:3103001/1‑101 (MQ=37)
                                                                                                     |                                                                                                      
ATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCC.TGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGCG.ACGGGCGGGGAAAGGCGA  >  NC_014034/1612328‑1612528

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG