Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,613,975 T→C 3.8% intergenic (+138/+89) rnd2 → / ← uvrD ribonuclease D/DNA helicase II

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,613,9750T→C 3.8% 2.1 ‑0.6 ‑0.0106intergenic (+138/+89)rnd2/uvrDribonuclease D/DNA helicase II
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (30/72):  total (30/76)
Fisher's exact test strand distribution p-value = 5.75e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.64e-01

Only 100 of 132 total aligned reads displayed.

GCATTTCCCCCTGCCCGCCGAGGGCCCAAA.GG.CCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCA  >  NC_014034/1613877‑1614074
                                                                                                      |                                                                                                       
gcttcccccccCGCCCGCCGAGGCCCAAAA.GC.CCCTCGCCCGGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTttg                                                                                                       <  2:3610209/95‑1 (MQ=17)
  atcccccccTGCCCGCGGAGGCCCCAAA.GG.CCCTGGGCCTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTttg.tt                                                                                                    <  1:2772937/97‑1 (MQ=17)
   ttccccccCGCCGGGCGGGGGCCAAAA.GA.CCCGGGGCGTGCGGCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTttg.ttg                                                                                                   <  1:1126384/98‑1 (MQ=14)
         ccTGCCCGCCGAGGGCCCAAA.GG.CCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGGGGCGTCGCG.CCGCCCGGGGGGGGGGCCGGGCCTTTT.TTGGGCtg                                                                                              >  1:4130850/1‑100 (MQ=2)
            ccccGCCGGGCGCCCAAA.GC.CCCCGGCCCTGCCCCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTgggg                                                                                          <  1:3279009/100‑1 (MQ=14)
             cccGCCGGGGGCCAAAG.GG.CCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.a                                                                                        <  1:1157078/101‑1 (MQ=255)
                gccgGGGCCCCAAG.GG.CCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAAc                                                                                     <  1:3532177/101‑1 (MQ=255)
                 ccgGGGGCCAAAG.GC.CCCGCGGCCTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACg                                                                                    <  2:3923175/101‑1 (MQ=17)
                 ccgAGGGCCCAAA.GG.CCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGGGGGGTCGCGCCCC.CCGGGGGGGGGGGGGGGCCCTTG.TTG.GCTTggggggaag                                                                                     >  1:2658442/1‑96 (MQ=2)
                 ccgAGGGCCCAAA.GG.CCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGGGGCGTCGCG.CCGCCCGGGGGGGGGGGCGGGCCTTT..TTGGGCTTtggggggag                                                                                     >  1:1780573/1‑96 (MQ=2)
                     gggCCCAAG.GG.CCCCGGGCCTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCt                                                                                <  1:3376818/101‑1 (MQ=255)
                     gggCCCAAA.GGCCCCTCGCCCGGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTc                                                                                 <  2:2899423/101‑1 (MQ=17)
                     gggCCAAAA.GG.CCCTGGGCCTGCCCCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCt                                                                                <  1:2024605/101‑1 (MQ=255)
                      gccccAAA.GG.CCCCCGCCCTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCtt                                                                               <  2:3197684/99‑1 (MQ=255)
                       gCCCAAAGGG.CCCTGGGCCTGCCCCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCtt                                                                               <  2:3614989/101‑1 (MQ=17)
                       gCCCAAA.GG.CCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGGGCACTCGCC.CCG.CCCGGGGGGGGGGGCGGCCCTTG.TTTCGCTCGGGG.GGACgggcgtt                                                                              >  2:578258/1‑96 (MQ=2)
                          cAAA.GG.CCCTCGCCCTGCCCCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCcg                                                                           <  2:1170347/101‑1 (MQ=255)
                            aa.GG.CCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGCGGCTTCGCG.CCGCCCGGGGGGGGGGCCGGGCCTTTT.TTG.GCCTGGGG.GAAAGGGCTTGCctcg                                                                         >  2:1256094/1‑101 (MQ=2)
                             aaggccccCCGGCCTCCCCCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCcgc                                                                          <  2:1660556/96‑1 (MQ=17)
                             aagcccccCCGCCCGGCCCCCGCCCCGCCATCGGCGGGCGCAGTCGCG.CCG.CCGGGGCGGAGCGCAGGCCTCTG.TTGTGCTTGGGGCAGGCGGTCGTGCcg                                                                           <  2:1078438/96‑1 (MQ=17)
                                   ccccgcccggcgcCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGt                                                                    <  2:25545/92‑1 (MQ=255)
                                      cgcccTGCCCCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGc                                                                 <  1:3150677/98‑1 (MQ=255)
                                       ggCCTGCGCCCGCCCCGCCATCGGCGGGTGGGTTCGCG.CGG.CCGGGGCGGGGGGCCGGGCGTTGTTTTCGTTTTGGG..GAGGGTCTTGTCGCGGGTGTTgtg                                                                >  1:2715634/1‑99 (MQ=2)
                                       ggCCTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCg                                                                <  1:194023/101‑1 (MQ=255)
                                       ggCCTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCg                                                                <  1:3475220/101‑1 (MQ=255)
                                         ccggcccccgccccgcCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.At                                                             <  1:1283370/95‑1 (MQ=255)
                                         ccTGCGCCCGCCCCGCCATCGGCGGGGGGATTCGCG.CCG.CCCGGGGGGGGGGCAGGGCCTTT.TTTCGCTTGGGG.GGAAGGGCTTGCCGCGGCCGGTGCggg                                                              >  1:2644888/1‑99 (MQ=9)
                                         ccTGCGCCCGCCCCGCCATCGGCGGGGGCCCTCGCG.CCC.CCCCGGGGGGGCGCGGGCCCTTT.TTTTGCTTTGGG.GGAGGGGGTTGCCGCGGCGGtggtgcg                                                              >  1:881233/1‑95 (MQ=2)
                                           tGCGCCCTCCCCGCCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.gc                                                          <  2:3404137/101‑1 (MQ=255)
                                             cccccgccccgcCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.gcgg                                                        <  1:2524393/99‑1 (MQ=255)
                                               cccgccccgcCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.gcgggc                                                      <  2:1153094/101‑1 (MQ=255)
                                                 cgccccgccATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.gcgggcgg                                                    <  2:4131134/101‑1 (MQ=255)
                                                   ccccgccATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCgggg                                                  <  1:1701793/101‑1 (MQ=255)
                                                         cATGGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGc                                            <  1:2872640/101‑1 (MQ=255)
                                                             ggcgggcgCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGGAGTg                                        >  2:1477158/1‑101 (MQ=255)
                                                             ggcgggcgCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTg                                        <  2:136521/101‑1 (MQ=255)
                                                              gcgggcgCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGc                                       <  1:3236022/101‑1 (MQ=255)
                                                               cgggcgCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGcc                                      >  2:1148540/1‑101 (MQ=255)
                                                                gggcgCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGccc                                     <  1:3937747/101‑1 (MQ=255)
                                                                 ggcgCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCt                                    >  2:3918595/1‑101 (MQ=255)
                                                                  gcgcATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCtt                                   <  2:2277145/101‑1 (MQ=255)
                                                                    gcATTGGCG.CCG.CCGGGGCGGGACGCAGGTCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTcc                                 <  2:1483956/101‑1 (MQ=255)
                                                                    gcATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTcc                                 >  1:2378943/1‑101 (MQ=255)
                                                                    gcATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTcc                                 >  2:2142513/1‑101 (MQ=255)
                                                                    gcATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTcc                                 >  2:3021192/1‑101 (MQ=255)
                                                                    gcATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTcc                                 <  1:3606783/101‑1 (MQ=255)
                                                                    gcATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTcc                                 <  1:365695/101‑1 (MQ=255)
                                                                     cATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCt                                <  2:1953881/101‑1 (MQ=255)
                                                                     cATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCt                                <  1:495670/101‑1 (MQ=255)
                                                                     cATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCt                                <  2:4072670/101‑1 (MQ=255)
                                                                     cATTCGAG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGCGG.AAACGGTCTTCCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCt                                <  2:495044/101‑1 (MQ=255)
                                                                      aTTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAGGGCAGTGCCCTTCCTg                               <  2:1067604/101‑1 (MQ=255)
                                                                      aTTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTg                               >  2:636214/1‑101 (MQ=255)
                                                                      aTTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTg                               <  2:536324/101‑1 (MQ=255)
                                                                      aTTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTg                               <  1:3435426/101‑1 (MQ=255)
                                                                      aTTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTg                               <  1:148577/101‑1 (MQ=255)
                                                                      aTTCGCG.CCG.CCGGGGCGGGACGCAGGCATTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTg                               <  2:1226955/101‑1 (MQ=255)
                                                                        tCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGAt                             <  1:3727770/101‑1 (MQ=255)
                                                                         cgcg.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATc                            <  2:422964/101‑1 (MQ=255)
                                                                         cgcg.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATc                            <  1:3918855/101‑1 (MQ=255)
                                                                          gcgcggcgggggggGGGGACGAAGGCCTCTG.TCGTGCTTGGGG.AAACGGTCTTGGCACAGGTGATGCGCCA.GCGGGCGGGGAAAGGCAGTGCCTTTCCCGa                              <  2:2936998/91‑1 (MQ=21)
                                                                          gcg.cCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATcc                           >  1:924237/1‑101 (MQ=255)
                                                                           cccgcggggggggCGGGCCAAAGGCCTCTG.TCGTGCTTGGGG.AAACGGTCTTGGCACAGGTGATGCG.CCAGCGGGCGGGGAAAGGCAGTGCCTTTCCCGAt                             <  2:2467004/92‑1 (MQ=25)
                                                                            g.ccg.ccGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGc                         >  1:1870179/1‑101 (MQ=255)
                                                                             cgcggggggggCGGGCCAAAGGCCTCTG.TCGTGCTTGGGG.AAACGGTCTTGGCACAGGTGATGCGCCA.GCGGGCGGGGAAAGGCAGTGCCTTTCCCGATcc                           <  2:3571034/94‑1 (MQ=25)
                                                                               cg.ccgGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGccc                       >  2:3031439/1‑101 (MQ=255)
                                                                               cg.ccgGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGccc                       >  1:2551912/1‑101 (MQ=255)
                                                                               cg.ccgGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGccc                       <  1:2362563/101‑1 (MQ=255)
                                                                               cg.ccgGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGccc                       <  2:2715428/101‑1 (MQ=255)
                                                                                g.ccgGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCg                      >  1:3870495/1‑101 (MQ=255)
                                                                                g.ccgGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCg                      <  1:2878381/101‑1 (MQ=255)
                                                                                cggcgGGGCGGGACGCAGGGCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGccc                       <  1:3401838/98‑1 (MQ=255)
                                                                                  ccgGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGa                     >  1:876499/1‑101 (MQ=255)
                                                                                    ggggCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAAc                   <  2:598086/101‑1 (MQ=255)
                                                                                    ggggCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAAc                   >  2:3285994/1‑101 (MQ=255)
                                                                                     gggCGGGACGCAGGTCTTTG.TTGCGCTGGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAAcc                  <  2:4235511/101‑1 (MQ=255)
                                                                                     gggCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAAcc                  <  2:1780454/101‑1 (MQ=255)
                                                                                      ggCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCg                 >  1:1682719/1‑101 (MQ=255)
                                                                                        cGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCggg               <  1:1250866/101‑1 (MQ=255)
                                                                                        cGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCggg               >  2:3539298/1‑101 (MQ=255)
                                                                                         gggACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCgggg              >  2:4213828/1‑101 (MQ=255)
                                                                                          ggACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGc             <  2:762325/101‑1 (MQ=255)
                                                                                             cGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCa          <  1:809052/101‑1 (MQ=255)
                                                                                             cGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCa          <  2:3323981/101‑1 (MQ=255)
                                                                                             cGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGAG.AT.GCGGGCGGGGTAAGGCAGTGTCCCTCCTGATTCGCCCTAACCTGGGCTCa          >  2:705465/1‑101 (MQ=21)
                                                                                              gccggCGTTTG.TTGCGCTTGAGA.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCGGCCCGAACCGGGGCTCAg         <  2:3573578/98‑1 (MQ=18)
                                                                                              gCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAg         >  1:2884441/1‑101 (MQ=255)
                                                                                              gCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAg         >  2:3374061/1‑101 (MQ=255)
                                                                                               cAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGa        >  2:1481005/1‑101 (MQ=255)
                                                                                               cAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACAGGGGCTCAGa        >  2:3592363/1‑101 (MQ=255)
                                                                                                aGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGaa       >  1:569917/1‑101 (MQ=255)
                                                                                                aGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGaa       >  1:2196991/1‑101 (MQ=255)
                                                                                                aGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGaa       >  2:1732131/1‑101 (MQ=255)
                                                                                                 ggCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAAc      >  1:2921339/1‑101 (MQ=255)
                                                                                                  gCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACg     <  1:3567847/101‑1 (MQ=255)
                                                                                                   ccTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGACCCGGGGCTCAGAACgg    >  1:1909566/1‑101 (MQ=255)
                                                                                                   ccTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACgg    <  2:3918938/101‑1 (MQ=255)
                                                                                                   ccTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAACGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACgg    <  2:2163169/101‑1 (MQ=255)
                                                                                                    cTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGc   <  2:1168388/101‑1 (MQ=255)
                                                                                                     tttG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCa  >  1:1500723/1‑101 (MQ=255)
                                                                                                      |                                                                                                       
GCATTTCCCCCTGCCCGCCGAGGGCCCAAA.GG.CCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCG.CCGGGGCGGGACGCAGGCCTTTG.TTGCGCTTGGGG.AAACGGTCTTGCCGCGGCTGTTGCG.AT.GCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCA  >  NC_014034/1613877‑1614074

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG