Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,644,761 C→T 100% S78F (TCC→TTC)  nuoA → NADH‑quinone oxidoreductase subunit A

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,644,7610C→T100.0% 817.1 206S78F (TCC→TTC) nuoANADH‑quinone oxidoreductase subunit A
Reads supporting (aligned to +/- strand):  new base (109/97):  ref base (0/0):  total (109/97)

Only 100 of 212 total aligned reads displayed.

AAAGGTGTCCGCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGAATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTCCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCCTCTCGGTGCTG  >  NC_014034/1644663‑1644858
                                                                                                  |                                                                                                 
aaaGGTGTCCGCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCg                                                                                                 <  1:1712108/101‑1 (MQ=255)
aaaGGTGTCCGCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCg                                                                                                 <  2:1201563/101‑1 (MQ=255)
 aaGGTGTCCGCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGa                                                                                                >  1:2497416/1‑101 (MQ=255)
  ccctgcccGCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGAt                                                                                               >  2:3200953/7‑101 (MQ=255)
  aGGTGTCCGCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGAt                                                                                               >  1:3519878/1‑101 (MQ=255)
  aGGTGTCCGCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGAt                                                                                               >  1:2454880/1‑101 (MQ=255)
     tgtCCGCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTg                                                                                            <  2:2569235/101‑1 (MQ=255)
       tCCGCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGa                                                                                          >  1:712134/1‑101 (MQ=255)
       tCCGCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGa                                                                                          >  2:4071069/1‑101 (MQ=255)
        ccGCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGaa                                                                                         >  1:1189154/1‑101 (MQ=255)
        ccGCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGaa                                                                                         >  1:1268493/1‑101 (MQ=255)
         cGCCTATGAATGCGGCTTCAACGCCTTTGACGCCGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAg                                                                                        >  1:3376152/1‑101 (MQ=255)
          gCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTGATCATCTTCGATCTGGAAGt                                                                                       <  2:414233/101‑1 (MQ=255)
          gCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGt                                                                                       <  2:1517059/101‑1 (MQ=255)
           ccTATTAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTg                                                                                      <  2:3470008/101‑1 (MQ=255)
           ccTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTg                                                                                      <  2:1648077/101‑1 (MQ=255)
           ccTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTg                                                                                      >  2:3325868/1‑101 (MQ=255)
            cTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTgg                                                                                     >  2:117020/1‑101 (MQ=255)
              aTGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCg                                                                                   >  2:3720452/1‑101 (MQ=255)
              aTGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCg                                                                                   >  1:156729/1‑101 (MQ=255)
               tGAATGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGt                                                                                  >  1:308035/1‑101 (MQ=255)
                 aaTGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTc                                                                                >  1:1901297/1‑101 (MQ=255)
                 aaTGCGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTc                                                                                <  2:1715138/101‑1 (MQ=255)
                     cGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGt                                                                            <  2:2354514/101‑1 (MQ=255)
                     cGGCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGt                                                                            <  2:2109543/101‑1 (MQ=255)
                      ggCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGtt                                                                           >  1:2583066/1‑101 (MQ=255)
                      ggCTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGtt                                                                           >  1:1126227/1‑101 (MQ=255)
                        cTTCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTcc                                                                         <  1:2960770/101‑1 (MQ=255)
                          tCAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTcccc                                                                       <  1:658951/101‑1 (MQ=255)
                           cAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCt                                                                      >  1:3151973/1‑101 (MQ=255)
                           cAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCt                                                                      >  1:1669314/1‑101 (MQ=255)
                           cAACGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCt                                                                      <  1:1507451/101‑1 (MQ=255)
                            aaCGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGTTCTGGAAGTGGCGTTCCTGTTCCCCTg                                                                     >  1:3443403/1‑101 (MQ=255)
                             aCGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTgg                                                                    >  2:364461/1‑101 (MQ=255)
                              cGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGNGTTCCTGTTCNNNNnng                                                                   >  2:941832/1‑101 (MQ=255)
                              cGCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTggg                                                                   <  2:3532094/101‑1 (MQ=255)
                               gCCTTTGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGc                                                                  <  1:517489/101‑1 (MQ=255)
                                  tttGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGGCGATCCTGTTCATCATCTTCGCTGTGGAAGTGGCGTTCCTGTTCCCCTGGGCggg                                                               >  1:2047844/1‑100 (MQ=255)
                                    tGACGACGCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTgg                                                             <  2:444783/101‑1 (MQ=255)
                                        gacgCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCtt                                                         <  1:988892/101‑1 (MQ=255)
                                        gacgCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTCGGCGGTGGCCtt                                                         <  1:2299953/101‑1 (MQ=255)
                                         acgCGCGGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCttt                                                        <  1:2865027/101‑1 (MQ=255)
                                          cgcgcgGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTg                                                       <  1:2968442/101‑1 (MQ=255)
                                            cgcgGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGc                                                     <  1:1047226/101‑1 (MQ=255)
                                             gcgGATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCg                                                    <  2:712058/101‑1 (MQ=255)
                                               ggaTGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGAc                                                  >  1:824506/3‑101 (MQ=255)
                                                 aTGAGGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACAt                                                >  2:3578268/1‑101 (MQ=255)
                                                 aTGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACAt                                                >  1:2755241/1‑101 (MQ=255)
                                                   gAAGTTCGACGGACGGTTTTATCGGGTGTCGATCCTGTTCAGCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGt                                              <  2:3815982/101‑1 (MQ=255)
                                                     aGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCg                                            >  2:3179491/1‑101 (MQ=255)
                                                     aGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCg                                            <  2:3108730/101‑1 (MQ=255)
                                                     aGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCg                                            >  2:1756023/1‑101 (MQ=255)
                                                     aGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGGGGCCTTTGGCGACATGTCg                                            >  2:3847052/1‑101 (MQ=255)
                                                       ttCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGAt                                          <  1:3841873/101‑1 (MQ=255)
                                                        tCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATg                                         <  1:1061076/101‑1 (MQ=255)
                                                        tCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATg                                         <  1:3283292/101‑1 (MQ=255)
                                                          gACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGAc                                       <  2:4032968/101‑1 (MQ=255)
                                                          gACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGAc                                       >  2:182167/1‑101 (MQ=255)
                                                               gcgcTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCt                                  <  2:389404/101‑1 (MQ=255)
                                                               gcgcTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCt                                  <  1:3031454/101‑1 (MQ=255)
                                                                 gcTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTc                                >  2:1681997/1‑101 (MQ=255)
                                                                 gcTTTTATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTc                                <  1:3541324/101‑1 (MQ=255)
                                                                   ttttATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATTTCGATGACCGCCTTATg                              >  2:703662/1‑101 (MQ=255)
                                                                   ttttATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTg                              >  2:204348/1‑101 (MQ=255)
                                                                    tttATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTTCCTTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTgg                             >  2:1368704/1‑101 (MQ=255)
                                                                     ttATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGt                            >  2:3969423/1‑101 (MQ=255)
                                                                     ttATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGt                            >  2:2901990/1‑101 (MQ=255)
                                                                      tATCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTc                           >  1:60344/1‑101 (MQ=255)
                                                                       aTCTGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCg                          >  2:2505140/1‑101 (MQ=255)
                                                                        tCTGGTGTCGATTCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCga                         >  1:3025411/1‑101 (MQ=255)
                                                                          tGGTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCgatg                       >  1:4036210/1‑101 (MQ=255)
                                                                           ggTGTCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCgatga                      >  1:791690/1‑101 (MQ=255)
                                                                            gtgtCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCgatgat                     <  2:3021967/101‑1 (MQ=255)
                                                                             tgtCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGatgatg                    >  2:3221690/1‑101 (MQ=255)
                                                                             tgtCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGatgatg                    <  1:3698489/101‑1 (MQ=255)
                                                                             tgtCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGatgatg                    <  1:2374559/101‑1 (MQ=255)
                                                                               tCGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGgt                  <  1:3426577/101‑1 (MQ=255)
                                                                                cGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGgtg                 >  1:376561/1‑101 (MQ=255)
                                                                                cGATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGgtg                 >  2:1958649/1‑101 (MQ=255)
                                                                                 gATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGgtgt                >  2:1250142/1‑101 (MQ=255)
                                                                                 gATCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGgtgt                >  1:268896/1‑101 (MQ=255)
                                                                                  aTCCTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGtt               >  1:2787705/1‑101 (MQ=255)
                                                                                    ccTGTTCATTATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTcc             >  1:530480/1‑101 (MQ=255)
                                                                                     cTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCct            >  2:592/1‑101 (MQ=255)
                                                                                     cTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCct            <  1:454301/101‑1 (MQ=255)
                                                                                     cTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCct            <  2:4216137/101‑1 (MQ=255)
                                                                                     cTGTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCct            >  1:4068566/1‑101 (MQ=255)
                                                                                       gTTCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCctct          <  2:824425/101‑1 (MQ=255)
                                                                                        ttCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCCtctc         >  1:1429809/1‑101 (MQ=255)
                                                                                        ttCATCATCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCCtctc         <  1:3348502/101‑1 (MQ=255)
                                                                                         tcatcaTCTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCCTCTCg        >  1:4135455/1‑101 (MQ=255)
                                                                                            tcatcTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCCTCTCGGTg     <  2:3365970/101‑1 (MQ=255)
                                                                                             catcTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCCTCTCGGTGc    >  1:924731/1‑101 (MQ=255)
                                                                                             catcTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCCTCTCGGTGc    <  1:3969683/101‑1 (MQ=255)
                                                                                             catcTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCCTCTCGGTGc    <  1:1778965/101‑1 (MQ=255)
                                                                                             catcTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCCTCTCGGTGc    >  2:2532654/1‑101 (MQ=255)
                                                                                              atcTTCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCCTCTCGGTGCt   <  2:1002162/101‑1 (MQ=255)
                                                                                               tcttCGATTGGGAAGTGGCGCTCCTGTTCCCCTGGGCGGTGGGCTTTGGCGACATGTCGATAACCGCCTTCTGGTAGATGATTGTGTTCCTCTCGGTGCTg  <  2:658567/101‑1 (MQ=255)
                                                                                               tcttCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCCTCTCGGTGCTg  >  1:2211879/1‑101 (MQ=255)
                                                                                               tcttCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCCTCTCGGTGCTg  <  2:2236745/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
AAAGGTGTCCGCCTATGAATGCGGCTTCAACGCCTTTGACGACGCGCGAATGAAGTTCGACGTGCGCTTTTATCTGGTGTCGATCCTGTTCATCATCTCCGATCTGGAAGTGGCGTTCCTGTTCCCCTGGGCGGTGGCCTTTGGCGACATGTCGATGACCGCCTTCTGGTCGATGATGGTGTTCCTCTCGGTGCTG  >  NC_014034/1644663‑1644858

Base quality scores:  ATCG  < 3 ≤  ATCG  < 25 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG