Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,668,401	G→C	100%	intergenic (‑66/+16)	ilvH ← / ← ilvI	acetolactate synthase small subunit/acetolactate synthase large subunit

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,668,401	0	G→C	100.0%	73.7	20	intergenic (‑66/+16)	ilvH/ilvI	acetolactate synthase small subunit/acetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (20/0):  ref base (0/0):  total (20/0)

AGCCTTTCTTGATGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCAAGGGGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCGG  >  NC_014034/1668308‑1668490                                                                                              |                                                                                          aGCCTTTCTTGATGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCgg                                                                                  >  2:4165522/1‑101 (MQ=255)           gATGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTcac                                                                        >  1:355236/1‑101 (MQ=255)             tGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCacac                                                                      >  2:2704412/1‑101 (MQ=255)                       ggCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCgcggcg                                                            >  1:3377168/1‑101 (MQ=255)                          cATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACGCCCGCgcgggcccg                                                         >  1:3527111/1‑96 (MQ=21)                                              tACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATc                                     >  2:2313599/1‑101 (MQ=255)                                                cTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCgg                                   >  2:803251/1‑101 (MQ=255)                                                  gATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGcc                                 >  2:691810/1‑101 (MQ=255)                                                   aTTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCg                                >  1:2967280/1‑101 (MQ=255)                                                   aTTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCg                                >  2:3397801/1‑101 (MQ=255)                                                          ttGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGagcag                         >  2:4218535/1‑101 (MQ=255)                                                           tGCCGGAAATATCCCGGGGGGTCCGGGGGGC..CGCGCCCCCCGGCCCCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCCAGagcaga                        >  2:2720339/1‑100 (MQ=37)                                                            gCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAgcagca                       >  2:4141914/1‑101 (MQ=255)                                                               ggAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCAttt                    >  2:776762/1‑101 (MQ=255)                                                                   atatCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACAAAAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGtt                >  2:3659687/1‑101 (MQ=37)                                                                    tatCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTa               >  2:181318/1‑101 (MQ=255)                                                                     atCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCCTCGGCCGAGAGCAGCAGTTCGTTag              >  1:1013185/1‑100 (MQ=37)                                                                     atCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTAt              >  2:210119/1‑101 (MQ=255)                                                                           gggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCt        >  2:3094920/1‑101 (MQ=255)                                                                           gggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCt        >  1:121371/1‑101 (MQ=255)                                                                            ggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCtt       >  2:2501351/1‑101 (MQ=255)                                                                                 ccGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCgg  >  1:2078623/1‑101 (MQ=255)                                                                                              |                                                                                          AGCCTTTCTTGATGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCAAGGGGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCGG  >  NC_014034/1668308‑1668490 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG