Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,668,733 C→T 100% S477N (AGC→AAC)  ilvI ← acetolactate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,668,7330C→T100.0% 874.3 222S477N (AGC→AAC) ilvIacetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (108/114):  ref base (0/0):  total (108/114)

Only 100 of 231 total aligned reads displayed.

CCAGTTTCACGAAATCGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGCTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGTTGAT  >  NC_014034/1668635‑1668830
                                                                                                  |                                                                                                 
   gTTTCACGAAATCGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTc                                                                                              <  1:1826255/101‑1 (MQ=255)
         cGAAATCGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATg                                                                                        <  1:1308965/101‑1 (MQ=255)
         cGAAATCGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATg                                                                                        >  2:1574583/1‑101 (MQ=255)
          gAAATCGGCCGGGCTTTCCGACCAGCTTTGCGAATAACCCCGGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGa                                                                                       <  2:2090448/101‑1 (MQ=255)
           aaaTCGGGCGGGCTTTCCGACCAGTTTTGCGAATAGCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGaa                                                                                      <  1:2981699/101‑1 (MQ=255)
            aaTCGGGCGGGCTTTCCGACCAGCTTGGCGAATACCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAAc                                                                                     <  1:3031158/101‑1 (MQ=255)
                gggCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCt                                                                                 >  1:2761547/1‑101 (MQ=255)
                 ggaggccTTCCCGAACAGCTTTGCGAAAAACGCCCCCCGTGCAGAAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCtt                                                                                <  2:1146406/95‑1 (MQ=255)
                 ggCAGGCTTTCCGACCGGCTTGCCAAATAACGCCCCCCATGCGGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCtt                                                                                <  1:1133468/101‑1 (MQ=255)
                 ggCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCtt                                                                                >  1:1808693/1‑101 (MQ=255)
                 ggCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCtt                                                                                >  2:2322636/1‑101 (MQ=255)
                  gCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTg                                                                               >  2:728181/1‑101 (MQ=255)
                    aGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGAc                                                                             <  1:1972617/101‑1 (MQ=255)
                       cTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCgg                                                                          <  1:1898466/101‑1 (MQ=255)
                        tttCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCggg                                                                         >  2:2528444/1‑101 (MQ=255)
                        tttCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCggg                                                                         >  2:3264137/1‑101 (MQ=255)
                           ccGCCCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTg                                                                      <  1:2528628/101‑1 (MQ=255)
                             gcccAGCTTTGCGAATACCCCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCg                                                                    <  2:2215121/99‑1 (MQ=255)
                               ccAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGa                                                                  >  1:2200439/1‑101 (MQ=255)
                                cAGCTTTGCGAATACCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGaa                                                                 <  1:460677/101‑1 (MQ=255)
                                cAGCTTTGCGAAAAACGCCGGCCATGCAGGGGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGaa                                                                 <  1:829351/101‑1 (MQ=255)
                                cAGCTTGGAGAACAACGCCCGCTATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGaa                                                                 <  2:2718127/101‑1 (MQ=255)
                                 aGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAAc                                                                >  1:2438166/1‑101 (MQ=255)
                                  gttttGCGAAAACCCCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACt                                                               <  1:1574692/99‑1 (MQ=255)
                                   cTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTg                                                              >  1:1020955/1‑101 (MQ=255)
                                      tGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGt                                                           >  2:2960098/1‑101 (MQ=255)
                                      tGCGAATAACCCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGt                                                           <  2:1357099/101‑1 (MQ=255)
                                          aaTAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGcc                                                       >  1:3912374/1‑101 (MQ=255)
                                          aaTAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGcc                                                       >  2:1200997/1‑101 (MQ=255)
                                           atagcccccgccACGCAGAGGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCg                                                      <  1:4148563/95‑1 (MQ=255)
                                           aTAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCTGGGTGCGGAACTGCGTCGCCg                                                      >  1:2899773/1‑101 (MQ=255)
                                            aaaCCCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGt                                                     <  1:3879990/100‑1 (MQ=255)
                                             agccccccccATGCGCCGGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTg                                                    <  1:2306612/97‑1 (MQ=255)
                                             aaCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTg                                                    >  2:2396337/1‑101 (MQ=255)
                                             aaCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTg                                                    >  1:3174304/1‑101 (MQ=255)
                                             aaCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTg                                                    >  1:1684174/1‑101 (MQ=255)
                                             aaCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTg                                                    >  1:79497/1‑101 (MQ=255)
                                               cgcccgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGcc                                                  >  2:1461632/1‑101 (MQ=255)
                                                gcccgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGccc                                                 <  2:2511212/101‑1 (MQ=255)
                                                gcccgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGccc                                                 >  1:3657851/1‑101 (MQ=255)
                                                gcccgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGccc                                                 >  1:1560911/1‑101 (MQ=255)
                                                gcccgccAGGCAGCAGGCCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGccc                                                 <  1:3523635/101‑1 (MQ=255)
                                                cccccccGTGCAGCGGGCCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGccc                                                 <  1:3277025/100‑1 (MQ=255)
                                                 cccgccGTGCGGCAGTTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCa                                                <  1:2627885/101‑1 (MQ=255)
                                                  ccgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCAt                                               >  2:2107393/1‑101 (MQ=255)
                                                   cgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCAtt                                              >  1:2246573/1‑101 (MQ=255)
                                                   cgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCAtt                                              >  2:1171313/1‑101 (MQ=255)
                                                    gccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCAttt                                             >  2:845312/1‑101 (MQ=255)
                                                    gccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCAttt                                             <  1:4063076/101‑1 (MQ=255)
                                                    gccAGGCAGCAGTCCCGGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCAttt                                             <  2:3697738/101‑1 (MQ=255)
                                                     ccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTc                                            <  2:3154123/101‑1 (MQ=255)
                                                     ccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTc                                            >  2:1245034/1‑101 (MQ=255)
                                                     ccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTc                                            <  2:428436/101‑1 (MQ=255)
                                                      cATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTcc                                           >  2:3589706/1‑101 (MQ=255)
                                                      cATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTcc                                           >  2:2028277/1‑101 (MQ=255)
                                                       aTGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCt                                          <  1:34608/101‑1 (MQ=255)
                                                        tGCAGCGGGCCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTg                                         <  1:616790/101‑1 (MQ=255)
                                                        tGCAGCAGGCCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTg                                         <  2:2437990/101‑1 (MQ=255)
                                                         gcagcaGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGc                                        <  2:1718828/101‑1 (MQ=255)
                                                          cagcagGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCa                                       >  2:2659665/1‑101 (MQ=255)
                                                           agcagGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCAt                                      >  2:3407295/1‑101 (MQ=255)
                                                           agcagGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCAt                                      >  1:25565/1‑101 (MQ=255)
                                                             cagGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGGGCGGAACTGCGTCGCCGTGCCCATTTCCTGctttt                                    >  2:1901426/1‑97 (MQ=255)
                                                              gggTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGtt                                   <  2:615449/100‑1 (MQ=255)
                                                                gcccTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTtca                                 <  1:894384/99‑1 (MQ=255)
                                                                gTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTtca                                 <  1:1295204/101‑1 (MQ=255)
                                                                 tCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTtcat                                <  2:4144543/101‑1 (MQ=255)
                                                                 tCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTtcat                                >  2:692940/1‑101 (MQ=255)
                                                                 cccTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTtcat                                <  1:1241368/100‑1 (MQ=255)
                                                                   cTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTtcatca                              <  2:3574073/101‑1 (MQ=255)
                                                                   cTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTtcatca                              >  2:406794/1‑101 (MQ=255)
                                                                    tGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAg                             <  2:1808574/101‑1 (MQ=255)
                                                                      ccACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGcc                           >  2:3232563/1‑101 (MQ=255)
                                                                       cACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCa                          >  1:1472685/1‑101 (MQ=255)
                                                                        aCTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAg                         >  1:1068120/1‑101 (MQ=255)
                                                                         cTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAgg                        >  2:805396/1‑101 (MQ=255)
                                                                         cTGCCGCACCATGCCAAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAgg                        >  2:3521650/1‑101 (MQ=255)
                                                                          tGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGCACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGa                       >  2:1883194/1‑101 (MQ=255)
                                                                             cGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGc                    >  2:3740464/1‑101 (MQ=255)
                                                                              gCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGcc                   >  2:2151533/1‑101 (MQ=255)
                                                                               cACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCt                  >  2:3622366/1‑101 (MQ=255)
                                                                               cACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCt                  >  2:1509499/1‑101 (MQ=255)
                                                                                 ccATGCCCTGCCGCTCGTTGTTCAGGATGATCTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCa                <  2:2072952/101‑1 (MQ=255)
                                                                                 ccATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCa                <  2:3049656/101‑1 (MQ=255)
                                                                                 ccATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCa                <  1:2838738/101‑1 (MQ=255)
                                                                                 ccATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCa                <  1:401878/101‑1 (MQ=255)
                                                                                 ccATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCa                >  2:1685601/1‑101 (MQ=255)
                                                                                  cATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCAc               <  1:3248572/101‑1 (MQ=255)
                                                                                  cATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCAc               <  1:3418186/101‑1 (MQ=255)
                                                                                  cATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCAc               <  1:2092773/101‑1 (MQ=255)
                                                                                   aTGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCTGCCAGGACGCCTCAcc              <  1:3697653/101‑1 (MQ=255)
                                                                                    tGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAAATGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCg             >  1:3580360/1‑101 (MQ=255)
                                                                                     gCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCgg            >  1:1184183/1‑101 (MQ=255)
                                                                                     gCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCgg            >  2:636173/1‑101 (MQ=255)
                                                                                      cccAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGc           <  1:64819/101‑1 (MQ=255)
                                                                                       ccAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCg          <  1:719474/101‑1 (MQ=255)
                                                                                         aGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGAt        <  1:1500014/101‑1 (MQ=255)
                                                                                            cgctcgttGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGCTCATCAGCCAGGACGCCTCACCGGCGATGtt     <  1:3751835/101‑1 (MQ=255)
                                                                                             gctcgttGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGTTg    >  2:3846621/1‑101 (MQ=255)
                                                                                              ctcgttGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGTTGa   <  1:2107531/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CCAGTTTCACGAAATCGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGCTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGTTGAT  >  NC_014034/1668635‑1668830

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG