Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,668,994 C→T 100% S390N (AGC→AAC)  ilvI ← acetolactate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,668,9940C→T100.0% 654.6 169S390N (AGC→AAC) ilvIacetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (93/76):  ref base (0/0):  total (93/76)

Only 100 of 192 total aligned reads displayed.

CCAGCCCTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGCTGCGCCCCTTGGTCAGGGCCTTCAGCCGCTCCAGCGCATATTGCGGTTTGGTGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTC  >  NC_014034/1668896‑1669089
                                                                                                  |                                                                                               
  aGCCCTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTgcg                                                                                             >  2:1620839/1‑101 (MQ=255)
     ccTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGccc                                                                                          <  1:101455/101‑1 (MQ=255)
     ccTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGccc                                                                                          >  2:3621366/1‑101 (MQ=255)
     ccTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGccc                                                                                          <  1:3245101/101‑1 (MQ=255)
      cTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGcccc                                                                                         >  1:2118579/1‑101 (MQ=255)
       tCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCt                                                                                        <  1:1035633/101‑1 (MQ=255)
        ccGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCtt                                                                                       >  2:2469980/1‑101 (MQ=255)
        ccGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCtt                                                                                       <  1:521135/101‑1 (MQ=255)
         cGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTg                                                                                      <  2:3657598/101‑1 (MQ=255)
         cGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTg                                                                                      <  2:1993911/101‑1 (MQ=255)
         cGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTg                                                                                      <  2:1083230/101‑1 (MQ=255)
           cTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGt                                                                                    >  2:4120461/1‑101 (MQ=255)
            tGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTGGGTGGTGATGTAGCGGTTGCGCCCCTTGGTc                                                                                   <  1:3749197/101‑1 (MQ=255)
            tGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTc                                                                                   <  2:921563/101‑1 (MQ=255)
             ggTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCa                                                                                  <  1:1883314/101‑1 (MQ=255)
             ggTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCa                                                                                  <  1:3521903/101‑1 (MQ=255)
             ggTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGGCa                                                                                  >  2:1766717/1‑101 (MQ=255)
              gTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAg                                                                                 <  1:1186043/101‑1 (MQ=255)
              gTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAg                                                                                 <  2:3199707/101‑1 (MQ=255)
               tCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAgg                                                                                >  1:575056/1‑101 (MQ=255)
               tCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAgg                                                                                >  1:1664857/1‑101 (MQ=255)
               tCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAgg                                                                                >  2:4090907/1‑101 (MQ=255)
                 aTCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGc                                                                              >  2:747340/1‑101 (MQ=255)
                  tCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGcc                                                                             >  2:1421886/1‑101 (MQ=255)
                  tCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGcc                                                                             <  1:3820889/101‑1 (MQ=255)
                    cAGCGGCTCGGCCCCGCGAAGCCCCGATATTGCGCCGCCCGCGTCTGATGCTGGCGGACGTCGGTGGTTATGTAGCGGTTGCGCCCCTTGGTCAGGGCCtc                                                                           <  2:2616334/101‑2 (MQ=255)
                     aGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCtcc                                                                          <  1:3974621/101‑3 (MQ=255)
                     aGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCtcc                                                                          <  2:3073980/101‑3 (MQ=255)
                       cGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAg                                                                        <  1:2184620/101‑1 (MQ=255)
                          ttCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCg                                                                     >  2:4007817/1‑101 (MQ=255)
                          ttCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCg                                                                     <  1:2515836/101‑1 (MQ=255)
                           tCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGc                                                                    <  2:3431413/101‑1 (MQ=255)
                            cGGCGCCTCGAAGCCCAGATATTGCGCCGCCCGCATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCt                                                                   <  1:608537/101‑1 (MQ=255)
                            cGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCt                                                                   >  2:2785258/1‑101 (MQ=255)
                            cGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCt                                                                   <  1:2834095/101‑1 (MQ=255)
                            cGGCGCCTCGAAGCCCAGATAGTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCt                                                                   >  1:2404003/1‑101 (MQ=255)
                               cgcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCa                                                                >  1:2081854/1‑101 (MQ=255)
                               cgcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCa                                                                >  1:2759155/1‑101 (MQ=255)
                               cgcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCa                                                                >  1:2857419/1‑101 (MQ=255)
                                 ccTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgc                                                              <  2:2117038/101‑1 (MQ=255)
                                 ccTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgc                                                              <  2:2660550/101‑1 (MQ=255)
                                 ccTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgc                                                              <  2:1173582/101‑1 (MQ=255)
                                 ccTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgc                                                              <  2:3936834/101‑1 (MQ=255)
                                  cTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTTAGGGCCTCCAGCCGCTCCAgcg                                                             <  2:3108735/101‑1 (MQ=255)
                                    cGAAGCCCAGATATTGCGCCTCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCa                                                           >  1:4160903/1‑101 (MQ=255)
                                     gAAGCCGAAATATGGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCat                                                          <  2:1494286/101‑1 (MQ=255)
                                     gAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCat                                                          <  1:1950308/101‑1 (MQ=255)
                                     gAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCATCCAGCCGCTCCAGCGCat                                                          >  1:1085501/1‑101 (MQ=255)
                                      aaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCata                                                         >  2:2932247/1‑101 (MQ=255)
                                      aaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCata                                                         >  1:811387/1‑101 (MQ=255)
                                      aaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCata                                                         >  1:3211562/1‑101 (MQ=255)
                                       aGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCatat                                                        <  1:2042895/101‑1 (MQ=255)
                                       aGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCatat                                                        >  1:308525/1‑101 (MQ=255)
                                       aGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCatat                                                        <  2:2317953/101‑1 (MQ=255)
                                       aGCCCAGATATTGCGCCGCCCACATCNGATNCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCatat                                                        <  1:3354262/101‑1 (MQ=255)
                                        gCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGGGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATAtt                                                       >  2:730699/1‑101 (MQ=255)
                                         cccAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTg                                                      >  2:1716615/1‑101 (MQ=255)
                                          ccAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGc                                                     >  1:403805/1‑101 (MQ=255)
                                           cAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCg                                                    >  1:1701744/1‑101 (MQ=255)
                                            aGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCgg                                                   >  2:1505245/1‑101 (MQ=255)
                                              atatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTACGGGCCTCCAGCCGCTCCAGCGCATATTGCGGtt                                                 >  1:3630341/1‑101 (MQ=255)
                                                atTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTg                                               >  2:3243538/1‑101 (MQ=255)
                                                atTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTg                                               >  1:4130466/1‑101 (MQ=255)
                                                atTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTg                                               >  1:4135036/1‑101 (MQ=255)
                                                atTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTg                                               <  1:868003/101‑1 (MQ=255)
                                                atTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTg                                               >  1:94605/1‑101 (MQ=255)
                                                      ccgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTTAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATg                                         <  1:3503978/101‑1 (MQ=255)
                                                       cgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGc                                        >  2:3038256/1‑101 (MQ=255)
                                                        gccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCt                                       <  2:3523461/101‑1 (MQ=255)
                                                        gccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCt                                       >  1:3856992/1‑101 (MQ=255)
                                                         cccACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTg                                      <  2:3856733/101‑1 (MQ=255)
                                                         cccACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTg                                      >  2:3243084/1‑101 (MQ=255)
                                                          ccACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGt                                     >  1:455215/1‑101 (MQ=255)
                                                          ccACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGt                                     <  1:747417/101‑1 (MQ=255)
                                                            acaTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTcc                                   <  2:1069321/101‑1 (MQ=255)
                                                             caTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCg                                  >  2:355956/1‑101 (MQ=255)
                                                             caTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCg                                  >  2:3344214/1‑101 (MQ=255)
                                                             caTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCg                                  <  2:4032070/101‑1 (MQ=255)
                                                              aTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGa                                 <  2:852954/101‑1 (MQ=255)
                                                               tCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAg                                >  2:3734229/1‑101 (MQ=255)
                                                                cTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGc                               >  2:603101/1‑101 (MQ=255)
                                                                    tGCGGGCCGACTGCGGTGGTGATGTAGCGGTTGCGCCACTTGGTCAGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGgt                           <  1:3612836/101‑1 (MQ=255)
                                                                       tGGCCGTCTTCGGTGGTTATGTACCGGTTGCCCCCCTTGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTa                        <  2:4160641/101‑1 (MQ=255)
                                                                       tGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTa                        >  2:3066039/1‑101 (MQ=255)
                                                                        ggCGGATTTCGGGGGTGGTGTAGCGGTTGCCCCCCTTGGTCAGGGCCCCCAGCCGCTCCAGCGCATATTGTGGTTTGATGATGCTGTCCGAGCCGGTGTAg                       <  2:1078992/101‑1 (MQ=255)
                                                                          ccGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGc                     <  2:41566/101‑1 (MQ=255)
                                                                           cgccTTGGGTGGTGATGTAGGGGTTGCCCCCCTGGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCg                    <  2:4061906/98‑1 (MQ=255)
                                                                            gACTTCGGTGTTGATTTAGCGTTTGCCCCCCTTGGTCAGGCCCTCCAGCCGCTCCATCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGa                   <  1:1620948/101‑1 (MQ=255)
                                                                            gACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGa                   <  1:2277699/101‑1 (MQ=255)
                                                                              cTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAgg                 >  2:2673454/1‑101 (MQ=255)
                                                                              cTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAgg                 >  1:910758/1‑101 (MQ=255)
                                                                               ttCGGTGGTGATGTAGCGTTTGCGCCCCTTGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGc                <  2:1816448/101‑1 (MQ=255)
                                                                               ttCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGc                >  1:1816568/1‑101 (MQ=255)
                                                                                    gggtgAGGTAGCGGTTGCGCCCCTTGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCgg           <  1:280251/100‑1 (MQ=255)
                                                                                     ggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCggg          >  2:428180/1‑100 (MQ=255)
                                                                                     ggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGa          >  2:3995002/1‑101 (MQ=255)
                                                                                      gtgATGTAGCGGTTGCGCCCCTTGGTCGGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGACCGAGCCGGTGTAGGCGAGGCAGCGGAt         <  1:1376668/101‑1 (MQ=255)
                                                                                      gtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAt         >  1:659767/1‑101 (MQ=255)
                                                                                        gATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtct       >  1:1958734/1‑101 (MQ=255)
                                                                                             aGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCttcttc  >  2:239822/1‑101 (MQ=255)
                                                                                                  |                                                                                               
CCAGCCCTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGCTGCGCCCCTTGGTCAGGGCCTTCAGCCGCTCCAGCGCATATTGCGGTTTGGTGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTC  >  NC_014034/1668896‑1669089

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG