Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,669,016 T→C 100% K383E (AAG→GAG)  ilvI ← acetolactate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,669,0160T→C100.0% 659.2 172K383E (AAG→GAG) ilvIacetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (101/71):  ref base (0/0):  total (101/71)

Only 100 of 186 total aligned reads displayed.

CGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGCTGCGCCCCTTGGTCAGGGCCTTCAGCCGCTCCAGCGCATATTGCGGTTTGGTGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGCCCCACCAT  >  NC_014034/1668919‑1669113
                                                                                                 |                                                                                                 
cGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAg                                                                                                <  1:2184620/101‑1 (MQ=255)
 gggtCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGc                                                                                               <  2:83353/98‑1 (MQ=255)
 ggTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGc                                                                                               >  2:4084913/1‑101 (MQ=255)
  gTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGcc                                                                                              >  2:3705521/1‑101 (MQ=255)
   ttCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCg                                                                                             >  2:4007817/1‑101 (MQ=255)
    tCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGc                                                                                            <  2:3431413/101‑1 (MQ=255)
     cGGCGCCTGGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCt                                                                                           <  2:656970/101‑1 (MQ=255)
     cGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGcc                                                                                           >  2:910129/1‑100 (MQ=255)
     cGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCt                                                                                           >  2:2785258/1‑101 (MQ=255)
     cGGCGCCTCGAAGCCCAGATAGTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCt                                                                                           >  1:2404003/1‑101 (MQ=255)
          ccTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgc                                                                                      <  2:2660550/101‑1 (MQ=255)
          ccTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgc                                                                                      <  2:1173582/101‑1 (MQ=255)
          ccTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgc                                                                                      >  1:2086755/1‑101 (MQ=255)
           cTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTTAGGGCCTCCAGCCGCTCCAgcg                                                                                     <  2:3108735/101‑1 (MQ=255)
             cGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCa                                                                                   >  1:3135857/1‑101 (MQ=255)
              gAAGCCGAAATATGGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCat                                                                                  <  2:1494286/101‑1 (MQ=255)
              gAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCat                                                                                  >  1:3053160/1‑101 (MQ=255)
              gAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCATCCAGCCGCTCCAGCGCat                                                                                  >  1:1085501/1‑101 (MQ=255)
               aaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCata                                                                                 >  1:2187516/1‑101 (MQ=255)
               aaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCata                                                                                 <  2:2202338/101‑1 (MQ=255)
                aGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCatat                                                                                <  1:2042895/101‑1 (MQ=255)
                aGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCatat                                                                                <  1:3514470/101‑1 (MQ=255)
                  cccAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTg                                                                              >  2:1716615/1‑101 (MQ=255)
                  cccAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTg                                                                              <  2:974256/101‑1 (MQ=255)
                   ccAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGGTGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCCGCCGCTCCAGCGCATATTGc                                                                             >  2:3779894/1‑101 (MQ=255)
                   ccAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGc                                                                             >  1:403805/1‑101 (MQ=255)
                     aGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCgg                                                                           >  2:1505245/1‑101 (MQ=255)
                      gATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGt                                                                          >  1:2508022/1‑101 (MQ=255)
                       atatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGtt                                                                         >  1:1497733/1‑101 (MQ=255)
                       atatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTACGGGCCTCCAGCCGCTCCAGCGCATATTGCGGtt                                                                         >  1:3630341/1‑101 (MQ=255)
                        tatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCGTATTGCGGttt                                                                        >  2:1243215/1‑101 (MQ=255)
                        tatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGttt                                                                        <  2:2095726/101‑1 (MQ=255)
                         atTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTg                                                                       >  1:94605/1‑101 (MQ=255)
                         atTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTg                                                                       <  1:868003/101‑1 (MQ=255)
                         atTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTg                                                                       <  1:3945069/101‑1 (MQ=255)
                         atTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCACTTGGTCAGGGCCTCCAGCAGCTCCGGCGCATATTGCGGTTTg                                                                       >  1:413162/1‑101 (MQ=255)
                           tGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTgat                                                                     <  1:604103/101‑3 (MQ=255)
                           tGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTgat                                                                     <  1:1934212/101‑3 (MQ=255)
                             cgccgcCCACATCTGATGCTGGCCGACTGCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGa                                                                   <  2:3636364/101‑1 (MQ=255)
                              gccgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGAt                                                                  >  2:3298832/1‑101 (MQ=255)
                              gccgccCACATCTGATGCTGGCCGACTTCGGTGGGGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTTCGGTTTGATGAt                                                                  >  2:4159413/1‑101 (MQ=255)
                               ccgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTTAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATg                                                                 <  1:3503978/101‑1 (MQ=255)
                                cgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGc                                                                >  2:3038256/1‑101 (MQ=255)
                                 gccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCt                                                               <  2:3523461/101‑1 (MQ=255)
                                  cccACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTg                                                              >  2:3243084/1‑101 (MQ=255)
                                   ccACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGt                                                             <  2:2989226/101‑1 (MQ=255)
                                   ccACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGt                                                             <  1:747417/101‑1 (MQ=255)
                                    cacaTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTc                                                            >  1:3836198/1‑101 (MQ=255)
                                     acaTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTcc                                                           <  2:1069321/101‑1 (MQ=255)
                                      caTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCg                                                          >  2:355956/1‑101 (MQ=255)
                                       aTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGa                                                         >  1:831163/1‑101 (MQ=255)
                                       aTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGa                                                         <  1:1719325/101‑1 (MQ=255)
                                         cTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGc                                                       <  1:2831553/101‑1 (MQ=255)
                                         cTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGc                                                       <  1:4085174/101‑1 (MQ=255)
                                          tGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGcc                                                      >  1:418615/1‑101 (MQ=255)
                                             tGCGGGCCGACTGCGGTGGTGATGTAGCGGTTGCGCCACTTGGTCAGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGgt                                                   <  1:3612836/101‑1 (MQ=255)
                                               cTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGgtgt                                                 >  2:390918/1‑101 (MQ=255)
                                                tGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTa                                                >  1:63517/1‑101 (MQ=255)
                                                tGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTa                                                >  2:3066039/1‑101 (MQ=255)
                                                 ggCGGATTTCGGGGGTGGTGTAGCGGTTGCCCCCCTTGGTCAGGGCCCCCAGCCGCTCCAGCGCATATTGTGGTTTGATGATGCTGTCCGAGCCGGTGTAg                                               <  2:1078992/101‑1 (MQ=255)
                                                 ggCCGACTTCGGTGGTGATGTAGCGTTTGCGCCCCTGGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAg                                               <  2:2403827/101‑1 (MQ=255)
                                                     gACTTCGGTGTTGATTTAGCGTTTGCCCCCCTTGGTCAGGCCCTCCAGCCGCTCCATCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGa                                           <  1:1620948/101‑1 (MQ=255)
                                                     gACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGa                                           >  1:8106/1‑101 (MQ=255)
                                                     gACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGa                                           <  1:2277699/101‑1 (MQ=255)
                                                      aCTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAg                                          >  1:478219/1‑101 (MQ=255)
                                                       cTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAgg                                         <  2:4130204/101‑1 (MQ=255)
                                                         tCGGTGGTGTTGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCa                                       <  2:459655/101‑1 (MQ=255)
                                                         tCGGTGGTGATGTAGCGGTTGCGCCCCTGGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCa                                       <  2:183864/101‑1 (MQ=255)
                                                          cGGTGGTGTTGTAGCGGTTGCCCCCTTTGGTCAGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAg                                      <  2:4196632/101‑1 (MQ=255)
                                                              ggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGa                                  >  2:3995002/1‑101 (MQ=255)
                                                              ggcgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGa                                  >  2:2710313/4‑101 (MQ=255)
                                                               gtgATGTAGCGGTTGCGCCCCTTGGTCGGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGACCGAGCCGGTGTAGGCGAGGCAGCGGAt                                 <  1:1376668/101‑1 (MQ=255)
                                                               gtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAt                                 >  1:659767/1‑101 (MQ=255)
                                                               gtgATGTAGCGGTTGCGCCCCTGGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAt                                 <  1:2773949/101‑1 (MQ=255)
                                                                 gATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtct                               >  1:1958734/1‑101 (MQ=255)
                                                                    gTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttct                            >  1:2852223/1‑101 (MQ=255)
                                                                    gTAGCGGTTGCGCCCCTTGGCCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttct                            <  2:167064/101‑1 (MQ=255)
                                                                    gTAGCGGTGGCCCCCCTGGGTCGGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttct                            <  2:3502369/101‑1 (MQ=255)
                                                                     tcgCGGTTCCGCCCCTTGTCCAGGGCCCCCAGCCGCTCCAGCCCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATcttctt                           <  1:1073052/99‑1 (MQ=255)
                                                                     tAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATcttctt                           >  1:2620399/1‑101 (MQ=255)
                                                                      aGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCttcttc                          >  2:239822/1‑101 (MQ=255)
                                                                          gtctcgcgctttGGCCTGGCCCTCCAGCCGATCCAGCGCATATTGCGGTTTGATGTGGCTGCCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCAct                      <  2:2758936/91‑1 (MQ=255)
                                                                           ttGCCCCCTTTGGTCGGGGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActc                     <  1:183157/100‑1 (MQ=255)
                                                                            tGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcc                    >  1:3758376/1‑101 (MQ=255)
                                                                            tGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcc                    >  2:1788349/1‑101 (MQ=255)
                                                                            tGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcc                    >  2:2813290/1‑101 (MQ=255)
                                                                              cgcCCCTTGGCAAGGCCCCCCAGCCGCTCCAGCGCATGTTGGTGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcctc                  <  2:4205410/101‑1 (MQ=255)
                                                                              ccccccTTGGCCAGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcctc                  <  1:4159675/99‑1 (MQ=255)
                                                                               gcCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCg                 >  1:2828498/1‑101 (MQ=255)
                                                                                ccccTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGa                >  1:1823796/1‑101 (MQ=255)
                                                                                ccccTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGa                >  1:1695098/1‑101 (MQ=255)
                                                                                  ccTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATc              >  1:1950379/1‑101 (MQ=255)
                                                                                     tGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTCGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTg           >  2:1042243/1‑101 (MQ=255)
                                                                                     tGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTg           >  2:526081/1‑101 (MQ=255)
                                                                                      ggTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGc          >  2:4050677/1‑101 (MQ=255)
                                                                                       gTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGcc         >  1:334183/1‑101 (MQ=255)
                                                                                         cAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGcccc       >  1:3230914/1‑101 (MQ=255)
                                                                                           gggCCCCCAGCCGCTCCAGCGCATTTTGGGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGCCccac     <  1:910214/101‑1 (MQ=255)
                                                                                            ggCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGCCccacc    >  2:2276227/1‑101 (MQ=255)
                                                                                              ccTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGCCCCACCAt  >  2:3993015/1‑101 (MQ=255)
                                                                                                 |                                                                                                 
CGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGCTGCGCCCCTTGGTCAGGGCCTTCAGCCGCTCCAGCGCATATTGCGGTTTGGTGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGCCCCACCAT  >  NC_014034/1668919‑1669113

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG