Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,696,166 A→C 100% intergenic (‑94/+131) RCAP_rcc01564 ← / ← mmsA isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,696,1660A→C100.0% 734.7 222intergenic (‑94/+131)RCAP_rcc01564/mmsAisobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (97/125):  ref base (0/0):  total (97/125)

Only 100 of 228 total aligned reads displayed.

AATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTC.G.G.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCG  >  NC_014034/1696066‑1696266
                                                                                                    |                                                                                                       
aaTCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTg                                                                                                       <  1:3031096/101‑1 (MQ=255)
   ccATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGcc                                                                                                    >  2:2564298/1‑101 (MQ=255)
    caagcgcTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCt                                                                                                   >  1:2414866/4‑101 (MQ=255)
    cATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCt                                                                                                   >  1:2481820/1‑101 (MQ=255)
       gcgcTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTc                                                                                                >  1:2532423/1‑101 (MQ=255)
       gcgcTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTc                                                                                                >  1:1194064/1‑101 (MQ=255)
       gcgcTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTc                                                                                                <  2:1150368/101‑1 (MQ=255)
        cgcTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.g                                                                                              >  2:2714409/1‑101 (MQ=255)
         gcTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.g.g                                                                                            >  2:861080/1‑101 (MQ=255)
           tcctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.G.cc                                                                                         <  1:1796298/101‑2 (MQ=255)
           tcctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.G.cc                                                                                         >  2:2000837/1‑100 (MQ=255)
             ctccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.G.ccaa                                                                                       >  1:3413884/1‑98 (MQ=255)
              tccCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.G.ccaat                                                                                      <  2:3761479/101‑5 (MQ=255)
               cccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.G.ccaatt                                                                                     <  2:3258439/101‑6 (MQ=255)
               cccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.G.ccaatt                                                                                     <  2:2340029/101‑6 (MQ=255)
               cccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.G.ccaatt                                                                                     >  1:2616819/1‑96 (MQ=255)
               cccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGCCCTTTC.G.G.ccaatt                                                                                     >  1:570062/1‑96 (MQ=255)
                ccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTc                                                                                     <  2:2210227/101‑1 (MQ=255)
                 cAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTca                                                                                    >  2:677068/1‑100 (MQ=255)
                 cAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTca                                                                                    <  2:2036576/101‑2 (MQ=255)
                  aaaGGGCCT.AAATGAACGTCTGTTTAATTTNGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.G.CCAATTCAcc                                                                                 <  1:1458385/101‑1 (MQ=255)
                   aaGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.G.CCAATTCAcc                                                                                 >  1:434946/1‑101 (MQ=255)
                    aGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.G.CCAATTCAccc                                                                                >  2:926325/1‑101 (MQ=255)
                     gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.G.CCAATTCAcccc                                                                               >  2:1747692/1‑100 (MQ=255)
                      tGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.G.CCAATTCAccccg                                                                              <  1:667089/101‑3 (MQ=255)
                       gCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.G.CCAATTCACCCcgg                                                                             <  1:452344/101‑1 (MQ=255)
                       gCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.G.CCAATTCACCCcgg                                                                             <  2:2642149/101‑1 (MQ=255)
                         cTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGa                                                                            <  1:4098406/101‑1 (MQ=255)
                         cTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGa                                                                            >  2:1376230/1‑101 (MQ=255)
                         cTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGa                                                                            >  2:641394/1‑101 (MQ=255)
                          tGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGat                                                                           >  1:3720990/1‑100 (MQ=37)
                          tGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGat                                                                           <  1:1676892/101‑2 (MQ=255)
                          tGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGat                                                                           >  2:355076/1‑100 (MQ=37)
                           gAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGatg                                                                          <  1:3067310/101‑3 (MQ=25)
                           gAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGatg                                                                          >  1:4011227/1‑99 (MQ=255)
                            aaTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGatgt                                                                         >  1:2305139/1‑98 (MQ=255)
                            aaTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGatgt                                                                         <  2:299402/101‑4 (MQ=255)
                             aTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGatgtt                                                                        <  2:1726741/101‑5 (MQ=255)
                              ttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGatgttt                                                                       <  2:1717555/101‑6 (MQ=25)
                                   cGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGcc                                                                  >  2:413712/1‑101 (MQ=25)
                                    gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCa                                                                 >  1:1133717/1‑101 (MQ=255)
                                       tGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCaagg                                                              >  1:1108984/1‑99 (MQ=255)
                                       tGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCaagg                                                              <  2:3947506/101‑3 (MQ=19)
                                        gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCaaggt                                                             <  2:295682/101‑4 (MQ=255)
                                        gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAAGgg                                                             >  1:3860893/1‑101 (MQ=255)
                                          ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCaaggtga                                                           <  2:401767/101‑6 (MQ=16)
                                          ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.CGCCCCTCCTGGCCTTTC.G.GCCAATTAACCCCGGGATTTTTGGGCCAAGggga                                                           >  1:356887/1‑100 (MQ=255)
                                           tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCaaggtgaa                                                          >  2:2143540/1‑95 (MQ=255)
                                            aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCaaggtgaaa                                                         <  1:3794677/101‑8 (MQ=255)
                                             aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAG.GGGGGGGC.TGCCCCTCCTGGCCTTTC.GCT.CAATTTACCCCGGGATGATTGGGCCaaggtggaac                                                        >  1:3463996/1‑93 (MQ=255)
                                               ttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacac                                                    <  1:4065108/101‑3 (MQ=255)
                                               ttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacac                                                    >  1:3243274/1‑99 (MQ=12)
                                                 cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGTGCCAA..GGTGAAacacct                                                  >  1:1706762/1‑97 (MQ=255)
                                                 cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacct                                                  >  2:2170299/1‑97 (MQ=255)
                                                 cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacct                                                  <  2:3330481/101‑5 (MQ=255)
                                                 cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacct                                                  >  1:3120526/1‑97 (MQ=255)
                                                  gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                 <  2:778379/101‑6 (MQ=255)
                                                  gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                 <  2:3443688/101‑6 (MQ=255)
                                                   aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                >  1:193247/1‑95 (MQ=255)
                                                   aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                <  2:1964853/101‑7 (MQ=12)
                                                    aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                               >  1:2229965/1‑94 (MQ=255)
                                                    aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                               >  2:3054120/1‑94 (MQ=255)
                                                      ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                             <  1:863606/101‑10 (MQ=9)
                                                       cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                            >  1:2091301/1‑91 (MQ=255)
                                                          gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                       <  1:955162/101‑1 (MQ=255)
                                                          gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                       <  1:4189620/101‑1 (MQ=255)
                                                            aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                     <  1:786670/101‑1 (MQ=9)
                                                               aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCACTTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTggg                                  >  1:2325921/1‑100 (MQ=255)
                                                               aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                                  <  1:3182710/101‑1 (MQ=9)
                                                               aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCCCCCGGCCTTTCTG.C.CAATTCACCCCCGGATGTTTGGGCCCA..GGTGGAA.CA.CCTTGCTTTTTCTTGgc                                  >  2:809034/1‑101 (MQ=255)
                                                                   ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTTGCGCaa                              >  1:3840992/1‑101 (MQ=255)
                                                                    tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                             >  2:51160/1‑101 (MQ=9)
                                                                    tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                             <  2:3720736/101‑1 (MQ=255)
                                                                      cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                           >  2:2434377/1‑101 (MQ=255)
                                                                      cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                           <  1:3495228/101‑1 (MQ=255)
                                                                      cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                           <  1:1735552/101‑1 (MQ=255)
                                                                       aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                          <  2:236406/101‑1 (MQ=255)
                                                                        aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCCA..GGCGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATTCg                         >  2:3860695/1‑101 (MQ=255)
                                                                        aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                         <  2:3685589/101‑1 (MQ=9)
                                                                        aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                         >  1:3660745/1‑101 (MQ=255)
                                                                         ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                        >  2:165009/1‑101 (MQ=255)
                                                                         ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                        >  2:1885795/1‑101 (MQ=255)
                                                                          gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                       <  1:413763/101‑1 (MQ=255)
                                                                           aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                      <  1:1315218/101‑1 (MQ=9)
                                                                           aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                      >  2:4073574/1‑101 (MQ=12)
                                                                             gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                    <  2:3104716/101‑1 (MQ=12)
                                                                             gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                    <  2:549700/101‑1 (MQ=255)
                                                                             gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                    <  1:1560730/101‑1 (MQ=255)
                                                                             gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                    <  1:214203/101‑1 (MQ=255)
                                                                                   gaggggcgctGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTTGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTTGGGCAAATACGCCTCTGGcc                >  1:3758137/10‑101 (MQ=255)
                                                                                    aggggcgctGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc               <  1:2714615/93‑1 (MQ=255)
                                                                                        gcgctGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg           <  1:1531263/97‑1 (MQ=255)
                                                                                         cgctGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg          <  1:2858079/98‑1 (MQ=16)
                                                                                          gctGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAggg         >  1:2482379/3‑101 (MQ=19)
                                                                                           ctGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc        >  2:2870644/2‑101 (MQ=16)
                                                                                            tGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa       <  2:3226963/101‑1 (MQ=16)
                                                                                            tGCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa       >  2:1248457/1‑101 (MQ=255)
                                                                                             gCCCCTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa      <  1:641471/101‑1 (MQ=255)
                                                                                              ccccTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg     <  2:1412046/101‑1 (MQ=19)
                                                                                              ccccTCCTGGCCTTTC.G.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg     >  1:1000892/1‑101 (MQ=19)
                                                                                                    |                                                                                                       
AATCCATGCGCTCCTCCCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTC.G.G.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCG  >  NC_014034/1696066‑1696266

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG