Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,696,184 C→A 91.3% intergenic (‑112/+113) RCAP_rcc01564 ← / ← mmsA isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,696,1840C→A91.3% 37.4 0.0 ‑0.1204intergenic (‑112/+113)RCAP_rcc01564/mmsAisobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (78/105):  ref base (8/11):  total (86/118)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.43e-01

Only 100 of 210 total aligned reads displayed.

AAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTC.GG.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCC  >  NC_014034/1696084‑1696285
                                                                                                      |                                                                                                     
 aaGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GG.CCAATTCAcc                                                                                                    >  1:434946/1‑101 (MQ=255)
 aaGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GG.CCAATTCAcc                                                                                                    <  2:3590879/101‑1 (MQ=255)
  aGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GG.CCAATTCAccc                                                                                                   >  2:926325/1‑101 (MQ=255)
   gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GG.CCAATTCAcccc                                                                                                  >  2:2670416/1‑100 (MQ=255)
     gCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GG.CCAATTCACCCcgg                                                                                                <  1:452344/101‑1 (MQ=255)
     gCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GG.CCAATTCACCCcgg                                                                                                >  2:2048056/1‑101 (MQ=37)
       cTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCTCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGa                                                                                               >  2:1236542/1‑101 (MQ=255)
       cTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGa                                                                                               >  2:641394/1‑101 (MQ=255)
       cTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGa                                                                                               >  1:536297/1‑101 (MQ=255)
        tGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGat                                                                                              <  1:3842990/101‑2 (MQ=255)
         gAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGatg                                                                                             <  1:3067310/101‑3 (MQ=25)
            ttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGatgttt                                                                                          <  2:1717555/101‑6 (MQ=25)
            ttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGatgttt                                                                                          >  2:4083874/1‑96 (MQ=255)
            ttGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGatgttt                                                                                          <  2:2924136/101‑6 (MQ=25)
             tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGTGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCGCCCCGGGATGTTTg                                                                                         <  1:838781/101‑1 (MQ=255)
             tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTg                                                                                         >  2:4064847/1‑101 (MQ=255)
             tGAACGTCTGTTTAATTTCGAATCCTAGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTg                                                                                         <  1:1858763/101‑1 (MQ=255)
               aaCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCCATTCACCCCGGGATGTTTggg                                                                                       >  1:154355/1‑101 (MQ=255)
                aCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGc                                                                                      <  1:3211974/101‑1 (MQ=255)
                aCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.AGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGc                                                                                      >  1:2482356/1‑101 (MQ=255)
                 cGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGcc                                                                                     <  1:4059378/101‑1 (MQ=255)
                   tCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCaa                                                                                   >  2:2330463/1‑101 (MQ=255)
                     tGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCaagg                                                                                 >  1:112763/1‑99 (MQ=255)
                      gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACTCCGGGATGTTTGGGCCaaggt                                                                                <  1:2405590/101‑4 (MQ=255)
                      gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCaaggt                                                                                >  1:1686309/1‑98 (MQ=255)
                        ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCaaggtga                                                                              <  1:4081391/101‑6 (MQ=16)
                        ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.CGCCCCTCCTGGCCTTTC.GGCCAATTAACCCCGGGATTTTTGGGCCAAGggga                                                                              >  1:356887/1‑100 (MQ=255)
                         tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCaaggtgaa                                                                             >  2:2143540/1‑95 (MQ=255)
                         tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCaaggtgaa                                                                             >  1:3470800/1‑95 (MQ=255)
                          aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCaaggtgaaa                                                                            <  1:4008949/101‑8 (MQ=255)
                          aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCaaggtgaaa                                                                            <  1:4101421/101‑8 (MQ=255)
                          aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCaaggtgaaa                                                                            <  1:3794677/101‑8 (MQ=255)
                            tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAaca                                                                        >  1:2382112/1‑100 (MQ=255)
                             ttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacac                                                                       >  1:3243274/1‑99 (MQ=12)
                             ttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacac                                                                       >  2:1742628/1‑99 (MQ=12)
                               cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacct                                                                     >  2:2170299/1‑97 (MQ=255)
                                gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                    <  1:926410/101‑6 (MQ=255)
                                gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                    <  2:778379/101‑6 (MQ=255)
                                 cgatcTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                   <  2:2574383/97‑7 (MQ=9)
                                 aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                   <  2:3495203/101‑7 (MQ=12)
                                 aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                   <  1:1311932/101‑7 (MQ=12)
                                 aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                   <  2:1964853/101‑7 (MQ=12)
                                  aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                  >  1:2229965/1‑94 (MQ=255)
                                  aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                  <  2:1296326/101‑8 (MQ=255)
                                  aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                  >  2:3054120/1‑94 (MQ=255)
                                    ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                                <  1:3498769/101‑10 (MQ=9)
                                     cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                               <  1:3728606/101‑11 (MQ=255)
                                     cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                               >  1:2091301/1‑91 (MQ=255)
                                        gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                          >  2:3743203/1‑101 (MQ=255)
                                        gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                          <  1:4189620/101‑1 (MQ=255)
                                         cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                                         >  2:3313167/1‑101 (MQ=9)
                                          aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                        <  2:53398/101‑1 (MQ=9)
                                           cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                       <  1:2317555/101‑1 (MQ=255)
                                             aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                                                     <  1:3182710/101‑1 (MQ=9)
                                             aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                                                     >  2:2386895/1‑101 (MQ=255)
                                             aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCCCCCGGCCTTTCTGC.CAATTCACCCCCGGATGTTTGGGCCCA..GGTGGAA.CA.CCTTGCTTTTTCTTGgc                                                     >  2:809034/1‑101 (MQ=255)
                                               ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc                                                   <  1:1570650/101‑1 (MQ=255)
                                               ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc                                                   >  1:2593942/1‑101 (MQ=9)
                                                 ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                 >  1:2793385/1‑101 (MQ=255)
                                                  tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                                <  2:3720736/101‑1 (MQ=255)
                                                  tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                                <  2:4000554/101‑1 (MQ=255)
                                                   gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                               <  2:2011600/101‑1 (MQ=9)
                                                   gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                               <  1:3811798/101‑1 (MQ=9)
                                                    cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                              <  1:1735552/101‑1 (MQ=255)
                                                    cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                              >  2:2434377/1‑101 (MQ=255)
                                                      aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCCA..GGCGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATTCg                                            >  2:3860695/1‑101 (MQ=255)
                                                      aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                            <  2:3685589/101‑1 (MQ=9)
                                                      aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                            >  1:3660745/1‑101 (MQ=255)
                                                        gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                          <  1:413763/101‑1 (MQ=255)
                                                        gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                          >  1:2264501/1‑101 (MQ=9)
                                                         aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGTCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                         >  2:1387883/1‑101 (MQ=12)
                                                         aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                         <  2:17495/101‑1 (MQ=9)
                                                           gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                       <  2:3104716/101‑1 (MQ=12)
                                                           gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                       >  1:150170/1‑101 (MQ=9)
                                                           gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                       <  2:549700/101‑1 (MQ=255)
                                                           gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                       <  1:214203/101‑1 (MQ=255)
                                                                 gaggggcgctGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTTGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTTGGGCAAATACGCCTCTGGcc                                   >  1:3758137/10‑101 (MQ=255)
                                                                   ggggcgctGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg                                 <  2:4164183/94‑1 (MQ=255)
                                                                     ggcgctGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGaa                               >  1:1084837/6‑101 (MQ=16)
                                                                      gcgctGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg                              <  1:1361192/97‑1 (MQ=255)
                                                                         ctGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc                           >  2:2870644/2‑101 (MQ=16)
                                                                          tGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                          <  1:785993/101‑1 (MQ=16)
                                                                          tGCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                          <  2:3226963/101‑1 (MQ=16)
                                                                           gCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                         <  1:641471/101‑1 (MQ=255)
                                                                           gCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                         >  2:162784/1‑101 (MQ=19)
                                                                           gCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                         >  2:2191513/1‑101 (MQ=19)
                                                                           gCCCCTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                         <  2:3342690/101‑1 (MQ=255)
                                                                            ccccTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGCAGGGCAAg                        >  2:1692870/1‑101 (MQ=16)
                                                                            ccccTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                        >  2:2977780/1‑101 (MQ=19)
                                                                            ccccTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                        >  1:1000892/1‑101 (MQ=19)
                                                                               cTCCTGGCCTTTC.GGCCAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcg                     <  1:2978002/101‑1 (MQ=21)
                                                                               cTCCTGGCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcg                     <  1:1186680/101‑1 (MQ=255)
                                                                                    ggCCTTTC.GGCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                >  2:1057942/1‑101 (MQ=255)
                                                                                            cggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcgc         >  2:2594750/5‑101 (MQ=255)
                                                                                             ggccAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGcgcg        <  1:3704033/98‑1 (MQ=25)
                                                                                               ccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGc      >  1:2693596/2‑101 (MQ=25)
                                                                                                cAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCTGCGCCGAAGGCGCGGcc     >  1:2508434/1‑101 (MQ=25)
                                                                                                cAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcc     >  2:1878215/1‑101 (MQ=255)
                                                                                                 aaTTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccc    >  2:855119/1‑101 (MQ=255)
                                                                                                   ttCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccccc  <  1:2622797/101‑1 (MQ=25)
                                                                                                      |                                                                                                     
AAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTC.GG.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCC  >  NC_014034/1696084‑1696285

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG