Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,696,193 A→T 100% intergenic (‑121/+104) RCAP_rcc01564 ← / ← mmsA isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,696,1930A→T100.0% 577.8 182intergenic (‑121/+104)RCAP_rcc01564/mmsAisobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (73/109):  ref base (0/0):  total (73/109)

Only 100 of 189 total aligned reads displayed.

TGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTC.GG.CAATTCCA.CCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCG  >  NC_014034/1696097‑1696292
                                                                                                   |                                                                                                   
 gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGTCTTTC.GGCCAATTCAC.CCCGGGATGTTTgg                                                                                               <  1:3764675/101‑1 (MQ=255)
 gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTgg                                                                                               <  2:870046/101‑1 (MQ=255)
   aCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.AGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGc                                                                                             >  1:2482356/1‑101 (MQ=255)
    cGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGcc                                                                                            <  1:4059378/101‑1 (MQ=255)
    cGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGcc                                                                                            >  2:2594102/1‑101 (MQ=25)
     gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCa                                                                                           >  1:1133717/1‑101 (MQ=255)
      tCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCaa                                                                                          >  2:2330463/1‑101 (MQ=255)
       cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGGGGGGC.GC.TGCCCCTCCTGGCCTTTCTGC.CGATTCGC.CCCGGGATGTTTTGGcccag                                                                                         >  1:3227202/1‑98 (MQ=255)
       cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCaag                                                                                         <  2:811550/101‑2 (MQ=255)
        tGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCaagg                                                                                        >  1:1108984/1‑99 (MQ=255)
        tGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCaagg                                                                                        <  2:3947506/101‑3 (MQ=19)
         gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.TCCGGGATGTTTGGGCCaaggt                                                                                       <  1:2405590/101‑4 (MQ=255)
         gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCaaggt                                                                                       >  1:1686309/1‑98 (MQ=255)
         gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCaaggt                                                                                       <  2:2878107/101‑4 (MQ=255)
          tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtg                                                                                      <  1:3929459/101‑5 (MQ=255)
           ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtga                                                                                     <  1:4081391/101‑6 (MQ=16)
           ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtga                                                                                     <  2:401767/101‑6 (MQ=16)
           ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.CGCCCCTCCTGGCCTTTC.GGCCAATTAAC.CCCGGGATTTTTGGGCCAAGggga                                                                                     >  1:356887/1‑100 (MQ=255)
            tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtgaa                                                                                    >  1:3470800/1‑95 (MQ=255)
                  cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGTGCCAA..GGTGAAacacct                                                                            >  1:1706762/1‑97 (MQ=255)
                  cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAAGgggaaacacct                                                                              >  1:65759/1‑93 (MQ=255)
                  cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacct                                                                            >  1:3120526/1‑97 (MQ=255)
                  cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacct                                                                            <  2:3330481/101‑5 (MQ=255)
                   gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                           <  2:778379/101‑6 (MQ=255)
                   gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                           <  2:939157/101‑6 (MQ=255)
                    cgatcTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                          <  2:2574383/97‑7 (MQ=9)
                    aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                          <  2:1964853/101‑7 (MQ=12)
                    aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                          >  1:193247/1‑95 (MQ=255)
                    aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                          <  1:1201293/101‑7 (MQ=12)
                    aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                          <  2:3495203/101‑7 (MQ=12)
                     aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                         >  1:2229965/1‑94 (MQ=255)
                      tCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgct                                                                        <  2:2154012/101‑9 (MQ=255)
                       ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                                       <  1:863606/101‑10 (MQ=9)
                       ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                                       <  1:3498769/101‑10 (MQ=9)
                           gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                                 <  1:4189620/101‑1 (MQ=255)
                           gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                                 <  1:955162/101‑1 (MQ=255)
                           gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                                 >  2:3743203/1‑101 (MQ=255)
                           gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                                 >  2:3075019/1‑101 (MQ=255)
                            cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                                                <  2:2532239/101‑1 (MQ=255)
                            cACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCt                                                                >  2:3313167/1‑101 (MQ=9)
                             aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                               <  2:53398/101‑1 (MQ=9)
                             aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                               <  2:1108890/101‑1 (MQ=9)
                             aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                               <  1:786670/101‑1 (MQ=9)
                                aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCACTTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTggg                                                            >  1:2325921/1‑100 (MQ=255)
                                  ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc                                                          >  1:2593942/1‑101 (MQ=9)
                                    ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTTGCGCaa                                                        >  1:3840992/1‑101 (MQ=255)
                                    ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                        >  1:2793385/1‑101 (MQ=255)
                                     tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                                       <  2:3720736/101‑1 (MQ=255)
                                       cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                     <  1:1735552/101‑1 (MQ=255)
                                       cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                     <  1:4119107/101‑1 (MQ=255)
                                        aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                                    <  2:236406/101‑1 (MQ=255)
                                         aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                                   <  2:3685589/101‑1 (MQ=9)
                                         aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                                   >  1:3660745/1‑101 (MQ=255)
                                          ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                                  >  2:3208885/1‑101 (MQ=255)
                                          ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                                  >  1:595900/1‑101 (MQ=255)
                                           gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                                 <  1:413763/101‑1 (MQ=255)
                                           gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                                 >  1:447266/1‑101 (MQ=9)
                                           gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                                 >  1:2264501/1‑101 (MQ=9)
                                            aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGTCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                >  2:1387883/1‑101 (MQ=12)
                                            aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                <  2:17495/101‑1 (MQ=9)
                                            aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                <  1:1315218/101‑1 (MQ=9)
                                            aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                >  2:4073574/1‑101 (MQ=12)
                                              gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                              <  1:1560730/101‑1 (MQ=255)
                                              gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                              <  1:214203/101‑1 (MQ=255)
                                              gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                              <  2:549700/101‑1 (MQ=255)
                                              gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                              >  1:150170/1‑101 (MQ=9)
                                              gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                              <  1:3730797/101‑1 (MQ=255)
                                                      ggggcgctGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg                                        <  2:4164183/94‑1 (MQ=255)
                                                         gcgctGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg                                     <  1:1531263/97‑1 (MQ=255)
                                                          cgctGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                                    <  1:2858079/98‑1 (MQ=16)
                                                          cgctGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                                    <  2:1723791/98‑1 (MQ=16)
                                                            ctGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc                                  <  2:2091163/100‑1 (MQ=255)
                                                             tGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                                 <  2:3226963/101‑1 (MQ=16)
                                                             tGCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                                 >  2:1248457/1‑101 (MQ=255)
                                                              gCCCCTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                                >  2:162784/1‑101 (MQ=19)
                                                               ccccTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGCAGGGCAAg                               >  2:1692870/1‑101 (MQ=16)
                                                               ccccTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                               >  1:1000892/1‑101 (MQ=19)
                                                               ccccTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                               >  2:2977780/1‑101 (MQ=19)
                                                               ccccTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                               >  1:3321665/1‑101 (MQ=16)
                                                               ccccTCCTGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTATTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                               >  2:2824071/1‑101 (MQ=16)
                                                                  cTCCTGGCCTTTC.GGCCAATTCCC.CCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcg                            <  1:2978002/101‑1 (MQ=21)
                                                                      tGGCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc                        <  1:2387071/101‑1 (MQ=19)
                                                                       ggCCTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAGACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                       <  1:3800518/101‑1 (MQ=255)
                                                                       ggCCTTTC.GG.CCAATCCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                       <  2:2414690/101‑1 (MQ=19)
                                                                          cTTTC.GGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg                    <  1:3209124/101‑1 (MQ=19)
                                                                                ggccAATTCCC.CCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGcgcg               <  1:3704033/98‑1 (MQ=25)
                                                                                ggccAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGcgcg               >  1:1035999/4‑101 (MQ=255)
                                                                                 gccAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCgg              <  2:3840733/99‑1 (MQ=25)
                                                                                  ccAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGc             >  1:2693596/2‑101 (MQ=25)
                                                                                   cAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCTGCGCCGAAGGCGCGGcc            >  1:2508434/1‑101 (MQ=25)
                                                                                   cAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcc            >  2:1878215/1‑101 (MQ=255)
                                                                                   cAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcc            <  2:2185880/101‑1 (MQ=25)
                                                                                     aTTCCC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccc          <  2:2025703/101‑1 (MQ=25)
                                                                                      ttCCC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccccc         <  1:2622797/101‑1 (MQ=25)
                                                                                         ccccccGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCGAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCt       <  2:2446638/98‑1 (MQ=255)
                                                                                         caccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCt       <  2:3413639/98‑1 (MQ=25)
                                                                                            cccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGAt    <  2:2229806/101‑1 (MQ=37)
                                                                                            cccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGAt    >  1:1826280/1‑101 (MQ=255)
                                                                                             ccGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTTTTTTTTCTTGGCGCAAATACCCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATc   <  1:2901012/101‑1 (MQ=255)
                                                                                             ccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATc   <  2:4019478/101‑1 (MQ=37)
                                                                                                   |                                                                                                   
TGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTC.GG.CAATTCCA.CCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCG  >  NC_014034/1696097‑1696292

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG