BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,696,195	G→T	100%	intergenic (‑123/+102)	RCAP_rcc01564 ← / ← mmsA	isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,696,195	0	G→T	100.0%	583.6	182	intergenic (‑123/+102)	RCAP_rcc01564/mmsA	isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand): new base (73/109): ref base (0/0): total (73/109)

Only 100 of 190 total aligned reads displayed.


TGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTC.G.G.CAATTCCA.CCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTC  >  NC_014034/1696097‑1696298


tGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGTGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCGC.CCCGGGATGTTTg                                                                                                      <  1:838781/101‑1 (MQ=255)


 gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGTCTTTC.G.GCCAATTCAC.CCCGGGATGTTTgg                                                                                                     <  1:3764675/101‑1 (MQ=255)


 gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTgg                                                                                                     <  2:870046/101‑1 (MQ=255)


   aCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGc                                                                                                   <  1:3211974/101‑1 (MQ=255)


    cGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGcc                                                                                                  >  2:2594102/1‑101 (MQ=25)


     gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCa                                                                                                 >  1:1133717/1‑101 (MQ=255)


      tCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCaa                                                                                                >  2:2330463/1‑101 (MQ=255)


       cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGGGGGGC.GC.TGCCCCTCCTGGCCTTTCTG.C.CGATTCGC.CCCGGGATGTTTTGGcccag                                                                                               >  1:3227202/1‑98 (MQ=255)


       cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCaag                                                                                               <  2:811550/101‑2 (MQ=255)


        tGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCaagg                                                                                              >  1:1108984/1‑99 (MQ=255)


        tGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCaagg                                                                                              <  2:3947506/101‑3 (MQ=19)


         gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCaaggt                                                                                             <  2:2878107/101‑4 (MQ=255)


         gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCaaggt                                                                                             >  1:1686309/1‑98 (MQ=255)


         gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAAGgg                                                                                             >  1:3860893/1‑101 (MQ=255)


          tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GACAATTCAC.CCCGGGAGGTTTGGGCCAAGgga                                                                                            >  2:1762370/1‑100 (MQ=255)


           ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCaaggtga                                                                                           <  2:401767/101‑6 (MQ=16)


           ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC..CCTGGCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGCTGTTTGGGCCaacgtga                                                                                           >  1:1723910/1‑96 (MQ=255)


             aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCaaggtgaaa                                                                                         <  1:4101421/101‑8 (MQ=255)


             aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCaaggtgaaa                                                                                         <  1:4008949/101‑8 (MQ=255)


              aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAG.GGGGGGGC.TGCCCCTCCTGGCCTTTC.GCT.CAATTTAC.CCCGGGATGATTGGGCCaaggtggaac                                                                                        >  1:3463996/1‑93 (MQ=255)


                ttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacac                                                                                    <  1:4065108/101‑3 (MQ=255)


                  cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAAGgggaaacacct                                                                                    >  1:65759/1‑93 (MQ=255)


                  cGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacct                                                                                  <  2:3330481/101‑5 (MQ=255)


                   gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                                 <  2:778379/101‑6 (MQ=255)


                   gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                                 <  1:926410/101‑6 (MQ=255)


                   gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                                 <  2:939157/101‑6 (MQ=255)


                    cgatcTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                <  2:2574383/97‑7 (MQ=9)


                    aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                >  1:193247/1‑95 (MQ=255)


                    aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                <  2:3495203/101‑7 (MQ=12)


                    aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttg                                                                                <  1:1201293/101‑7 (MQ=12)


                     aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                               >  1:2229965/1‑94 (MQ=255)


                      tCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgct                                                                              <  2:2154012/101‑9 (MQ=255)


                           gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                                       >  2:3075019/1‑101 (MQ=255)


                           gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                                       <  1:4189620/101‑1 (MQ=255)


                           gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                                       >  2:3743203/1‑101 (MQ=255)


                             aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                                     <  2:53398/101‑1 (MQ=9)


                                aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCACTTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTggg                                                                  >  1:2325921/1‑100 (MQ=255)


                                aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                                                                  <  1:3182710/101‑1 (MQ=9)


                                  ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc                                                                >  1:2593942/1‑101 (MQ=9)


                                    ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                              >  1:2793385/1‑101 (MQ=255)


                                     tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                                             <  2:3720736/101‑1 (MQ=255)


                                      gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                                            <  1:3811798/101‑1 (MQ=9)


                                      gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                                            <  2:2011600/101‑1 (MQ=9)


                                       cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                           >  2:2434377/1‑101 (MQ=255)


                                       cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                           <  1:1735552/101‑1 (MQ=255)


                                       cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                           <  1:3495228/101‑1 (MQ=255)


                                        aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                                          <  2:236406/101‑1 (MQ=255)


                                         aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                                         <  2:3685589/101‑1 (MQ=9)


                                          ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                                        >  2:1885795/1‑101 (MQ=255)


                                          ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                                        >  2:3208885/1‑101 (MQ=255)


                                          ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                                        >  1:595900/1‑101 (MQ=255)


                                           gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                                       >  1:2264501/1‑101 (MQ=9)


                                           gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                                       >  1:447266/1‑101 (MQ=9)


                                           gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                                       <  1:413763/101‑1 (MQ=255)


                                            aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GTCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                      >  2:1387883/1‑101 (MQ=12)


                                            aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                      >  2:4073574/1‑101 (MQ=12)


                                            aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                      <  1:1315218/101‑1 (MQ=9)


                                            aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                      <  2:17495/101‑1 (MQ=9)


                                              gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                                    <  1:1560730/101‑1 (MQ=255)


                                              gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                                    <  1:214203/101‑1 (MQ=255)


                                              gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                                    <  2:549700/101‑1 (MQ=255)


                                              gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                                    <  1:3730797/101‑1 (MQ=255)


                                              gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                                    >  1:150170/1‑101 (MQ=9)


                                                    gaggggcgctGCCTCTCCTGGCCTTTC.G.GCCAAGTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                                <  1:3640761/92‑1 (MQ=9)


                                                      ggggcgctGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg                                              <  2:3243033/94‑1 (MQ=255)


                                                      ggggcgctGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg                                              <  2:4164183/94‑1 (MQ=255)


                                                         gcgctGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg                                           <  1:1531263/97‑1 (MQ=255)


                                                         gcgctGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTAGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg                                           <  1:3559757/97‑1 (MQ=255)


                                                          cgctGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                                          <  1:2858079/98‑1 (MQ=16)


                                                          cgctGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                                          <  1:3046737/98‑1 (MQ=16)


                                                          cgctGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                                          <  2:1723791/98‑1 (MQ=16)


                                                           gctGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAggg                                         >  1:2482379/3‑101 (MQ=19)


                                                           gctGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAggg                                         <  1:2434553/99‑1 (MQ=255)


                                                            ctGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc                                        <  2:2091163/100‑1 (MQ=255)


                                                             tGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                                       <  1:785993/101‑1 (MQ=16)


                                                             tGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                                       >  2:1248457/1‑101 (MQ=255)


                                                             tGCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                                       <  2:3226963/101‑1 (MQ=16)


                                                              gCCCCTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                                      >  2:162784/1‑101 (MQ=19)


                                                               ccccTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGCAGGGCAAg                                     >  2:1692870/1‑101 (MQ=16)


                                                               ccccTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                     >  1:1000892/1‑101 (MQ=19)


                                                               ccccTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                     >  2:2977780/1‑101 (MQ=19)


                                                               ccccTCCTGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTATTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                     >  2:2824071/1‑101 (MQ=16)


                                                                      tGGCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc                              <  1:2387071/101‑1 (MQ=19)


                                                                       ggCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                             >  2:1057942/1‑101 (MQ=255)


                                                                       ggCCTTTC.G.GCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAGACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                             <  1:3800518/101‑1 (MQ=255)


                                                                       ggCCTTTC.G.G.CCAATCCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                             <  2:2414690/101‑1 (MQ=19)


                                                                                 ggccAATTCCC.CCCGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGcgcg                     <  1:3704033/98‑1 (MQ=25)


                                                                                 ggccAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGcgcg                     >  1:1035999/4‑101 (MQ=255)


                                                                                  gccAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCgg                    <  2:3840733/99‑1 (MQ=25)


                                                                                   ccAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGc                   >  1:2693596/2‑101 (MQ=25)


                                                                                    cAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCTGCGCCGAAGGCGCGGcc                  >  1:2508434/1‑101 (MQ=25)


                                                                                    cAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcc                  <  2:2185880/101‑1 (MQ=25)


                                                                                       ttCCC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccccc               <  1:2622797/101‑1 (MQ=25)


                                                                                       attcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccc                <  2:2481636/95‑1 (MQ=25)


                                                                                          caccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCt             <  2:3413639/98‑1 (MQ=25)


                                                                                             cccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGAt          <  2:2229806/101‑1 (MQ=37)


                                                                                             cccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGAt          >  1:1826280/1‑101 (MQ=255)


                                                                                              ccGGGATGTTTGGGCCAA..GGTGAAAC..CCCTTTTTTTTTCTTGGCGCAAATACCCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATc         <  1:2901012/101‑1 (MQ=255)


                                                                                              ccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATc         <  2:4019478/101‑1 (MQ=37)


                                                                                                      gTTGGGGCCAA.GGTGAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTc  <  1:3313408/101‑1 (MQ=37)


TGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTC.G.G.CAATTCCA.CCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGTGCCTC  >  NC_014034/1696097‑1696298

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG