Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,769,371 C→G 8.1% P148R (CCC→CGG)  RCAP_rcc01636 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,769,3710C→G 8.1% 15.5 ‑2.3 ‑0.6118P148R (CCC→CGC) RCAP_rcc01636hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/8):  ref base (53/55):  total (55/63)
Fisher's exact test strand distribution p-value = 1.02e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.44e-01

Only 100 of 119 total aligned reads displayed.

CGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTC.GGCA.GGG.GCGGTGGT.C.CC.CC.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCCG  >  NC_014034/1769285‑1769462
                                                                                           |                                                                                             
cGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCttt                                                                                  <  2:2202098/101‑1 (MQ=255)
cGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCttt                                                                                  <  1:3037034/101‑1 (MQ=255)
 ggCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTg                                                                                 >  1:3450347/1‑101 (MQ=255)
 ggCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTg                                                                                 <  1:1982205/101‑1 (MQ=255)
   cGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGGCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGcc                                                                               <  2:752383/101‑1 (MQ=255)
      tCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CTGGCTTTGCCTTg                                                                            <  1:2299382/101‑1 (MQ=255)
      gcAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTg                                                                            <  2:1572157/100‑1 (MQ=255)
       cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGTAG.CG.CG.CGGGCTTTGCCTTgg                                                                           >  2:2821239/1‑101 (MQ=255)
       cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTgg                                                                           >  2:1899126/1‑101 (MQ=255)
       cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTgg                                                                           >  2:235642/1‑101 (MQ=255)
       cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTgg                                                                           <  2:2643979/101‑1 (MQ=255)
       cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTgg                                                                           <  1:2233351/101‑1 (MQ=255)
        aGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.C.GCGCG.CGGGCTTTGCCTTggg                                                                          <  1:670479/101‑1 (MQ=255)
         ggACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTgggg                                                                         >  2:431995/1‑101 (MQ=255)
          gACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGt                                                                        <  2:2136656/101‑1 (MQ=255)
          gACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGt                                                                        <  1:2883474/101‑1 (MQ=255)
          gACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGt                                                                        <  2:3975372/101‑1 (MQ=255)
           aCCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGGG.CG.CGGGCTTTGCCTTGGGGTc                                                                       >  2:1937473/1‑101 (MQ=255)
           aCCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTc                                                                       >  2:1450826/1‑101 (MQ=255)
            ccAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCg                                                                      <  2:2937211/101‑1 (MQ=255)
            ccAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCg                                                                      >  2:2378010/1‑101 (MQ=255)
            ccAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.C.GCGCG.CGGGCTTTGCCTTGGGGTCg                                                                      <  1:4079433/101‑1 (MQ=255)
             cAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGa                                                                     <  2:3327859/101‑1 (MQ=255)
              aaCTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAg                                                                    <  2:778175/101‑1 (MQ=255)
              aaCTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAg                                                                    >  1:347767/1‑101 (MQ=255)
               aCTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.C.GCGCG.CGGGCTTTGCCTTGGGGTCGAgg                                                                   <  1:2598145/101‑1 (MQ=255)
                cTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgc                                                                  >  1:3423895/1‑101 (MQ=255)
                 tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcg                                                                 >  2:1208065/1‑101 (MQ=255)
                  ggATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcgc                                                                >  2:46243/1‑101 (MQ=255)
                  ggATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcgc                                                                >  1:3969553/1‑101 (MQ=255)
                  ggATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGGGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcgc                                                                >  2:1632331/1‑101 (MQ=255)
                   gATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCt                                                               >  1:1562735/1‑101 (MQ=255)
                   gATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCt                                                               <  2:2164029/101‑1 (MQ=255)
                   gATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCt                                                               <  2:3177130/101‑1 (MQ=255)
                    aTGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCtt                                                              <  1:468210/101‑1 (MQ=255)
                    aTGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTAGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCtt                                                              <  1:3641139/101‑1 (MQ=255)
                      gCGGCCCGCGCGGCGGAAATCGTCGCGGGTTTGGGCGCGGTCGATGTCGGGCA.GGGTGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTcc                                                            <  1:4216750/101‑1 (MQ=255)
                      gCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.C.GCGCG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTcc                                                            <  1:1068924/101‑1 (MQ=255)
                       cGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.C.GCGCG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCg                                                           <  1:1079022/101‑1 (MQ=255)
                        ggCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCgg                                                          >  1:3278945/1‑101 (MQ=255)
                          cccGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGc                                                        <  2:1134357/101‑1 (MQ=255)
                           ccGGGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.C.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCa                                                       <  2:676384/101‑1 (MQ=255)
                           ccGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.C.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCa                                                       <  2:2962569/101‑1 (MQ=255)
                           ccGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.C.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCa                                                       <  2:2605153/101‑1 (MQ=255)
                             gcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAcc                                                     >  1:765803/1‑101 (MQ=255)
                                cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  <  2:2998556/101‑1 (MQ=255)
                                cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTCCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  <  2:823470/101‑1 (MQ=255)
                                 ggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATg                                                 <  1:3814694/101‑1 (MQ=255)
                                    ggAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcgc                                              <  1:3714905/101‑1 (MQ=255)
                                     gAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCt                                             >  2:295746/1‑101 (MQ=255)
                                     gAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCt                                             >  2:1273430/1‑101 (MQ=255)
                                     gAAATCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCt                                             >  2:3905805/1‑101 (MQ=255)
                                        aTCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCt                                          <  2:3423650/101‑1 (MQ=255)
                                        aTCGTCGCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCt                                          >  2:3529993/1‑101 (MQ=255)
                                          cgtcgCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTg                                        >  1:1476272/1‑101 (MQ=255)
                                          cgtcgCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTg                                        >  1:3480116/1‑101 (MQ=255)
                                          cgtcgCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTg                                        <  1:783573/101‑1 (MQ=255)
                                           gtcgCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGa                                       >  1:3893975/1‑101 (MQ=255)
                                           gtcgCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGa                                       >  2:3743757/1‑101 (MQ=255)
                                           gtcgCGGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGa                                       <  1:2316397/101‑1 (MQ=255)
                                             cgcgGCTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                                     >  1:1395543/1‑101 (MQ=255)
                                                  cTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtc                                >  1:329194/1‑101 (MQ=255)
                                                  cTTTGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgcg                                >  2:4134382/1‑99 (MQ=255)
                                                   tttGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtcg                               >  2:3822110/1‑101 (MQ=255)
                                                   tttGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtcg                               >  1:4208167/1‑101 (MQ=255)
                                                     tGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgcc                             >  2:3082358/1‑101 (MQ=255)
                                                     tGGGCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgcc                             >  1:3419872/1‑101 (MQ=255)
                                                      gggCGCGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgccg                            >  2:247772/1‑101 (MQ=255)
                                                        gcgcGGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCcgcg                          >  1:2568216/1‑101 (MQ=255)
                                                          gcgGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCa                        >  1:2143524/1‑101 (MQ=255)
                                                          gcgGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCa                        >  2:3558179/1‑101 (MQ=255)
                                                           cgGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCAt                       <  2:1097712/101‑1 (MQ=255)
                                                           cgGTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCAt                       <  1:2094907/101‑1 (MQ=255)
                                                            ggTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATc                      <  2:1422703/101‑1 (MQ=255)
                                                             gTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcg                     >  1:1003139/1‑101 (MQ=255)
                                                             gTCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcg                     >  1:3714907/1‑101 (MQ=255)
                                                              tCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgc                    >  1:719352/1‑101 (MQ=255)
                                                              tCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgc                    >  2:1151696/1‑101 (MQ=255)
                                                              tCGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCAATGCGCTCCTTGATTTCGTCGCCGCGCATcgc                    >  2:816486/1‑101 (MQ=255)
                                                               cGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgcg                   <  1:2918290/101‑1 (MQ=255)
                                                               cGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgcg                   <  2:4044011/101‑1 (MQ=255)
                                                               cGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgcg                   >  1:1392039/1‑101 (MQ=255)
                                                               cGATGTCGGGCAGGGG.GCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgcg                   >  1:2453065/1‑101 (MQ=255)
                                                                  tgtcgggcagggg.gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGc                >  1:2909493/11‑101 (MQ=255)
                                                                  tgtcgggcagggg.gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGc                <  1:1937599/91‑1 (MQ=255)
                                                                  tgtcgggcagggg.gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGc                >  2:2367170/11‑101 (MQ=255)
                                                                    tcgggcagggg.gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTg              <  2:3357703/93‑1 (MQ=255)
                                                                      gggcagggg.gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGTCCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt            >  1:3630997/7‑101 (MQ=255)
                                                                      gggcagggg.gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTggg            >  2:2089058/7‑100 (MQ=255)
                                                                         cagggg.gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgcc         <  2:318402/98‑1 (MQ=255)
                                                                         cagggg.gCGGTGGT.C.GCGCG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgcc         >  2:3867327/4‑101 (MQ=255)
                                                                          agggg.gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccg        <  1:3593554/99‑1 (MQ=255)
                                                                           gggg.gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccga       >  1:3256464/2‑101 (MQ=255)
                                                                            ggg.gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgat      <  2:1050907/101‑1 (MQ=255)
                                                                            ggg.gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgat      <  1:2807747/101‑1 (MQ=255)
                                                                            ggg.gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgat      <  2:3235914/101‑1 (MQ=255)
                                                                             gg.gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatg     <  2:3893716/101‑1 (MQ=255)
                                                                                gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgcc   <  2:33990/101‑1 (MQ=255)
                                                                                gCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgcc   <  1:235680/101‑1 (MQ=255)
                                                                                 cGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgccg  <  1:3518630/101‑1 (MQ=255)
                                                                                           |                                                                                             
CGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGTC.GGCA.GGG.GCGGTGGT.C.CC.CC.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCCG  >  NC_014034/1769285‑1769462

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG