Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,770,647 C→G 100% P327A (CCC→GCC)  lpxB → lipid‑A‑disaccharide synthase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,770,6470C→G100.0% 622.4 166P327A (CCC→GCC) lpxBlipid‑A‑disaccharide synthase
Reads supporting (aligned to +/- strand):  new base (81/85):  ref base (0/0):  total (81/85)

Only 100 of 176 total aligned reads displayed.

GACGTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCGGCCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAACGACGA  >  NC_014034/1770550‑1770740
                                                                                                 |                                                                                             
gACGTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCTGAATTCCTCGGCCCGGCCt                                                                                            <  2:3433823/101‑1 (MQ=255)
gACGTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCt                                                                                            >  1:600056/1‑101 (MQ=255)
 aCGTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTg                                                                                           <  2:2381264/101‑1 (MQ=255)
   gTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTc                                                                                         >  2:1748592/1‑101 (MQ=255)
     ggCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCgg                                                                                       <  1:525017/101‑1 (MQ=255)
     ggCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCgg                                                                                       >  1:3172386/1‑101 (MQ=255)
     ggCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCgg                                                                                       >  2:2295155/1‑101 (MQ=255)
       cATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGcc                                                                                     >  1:694905/1‑101 (MQ=255)
       cATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGcc                                                                                     >  2:2794665/1‑101 (MQ=255)
        ttatCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCg                                                                                    <  2:1316136/100‑1 (MQ=255)
        atatCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCg                                                                                    <  2:766610/101‑1 (MQ=255)
          atCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGaa                                                                                  >  1:951699/1‑101 (MQ=255)
           tCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGaaa                                                                                 >  2:3172142/1‑101 (MQ=255)
             aCGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAAcc                                                                               <  1:3753943/101‑1 (MQ=255)
              cGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCg                                                                              >  1:2001848/1‑101 (MQ=255)
              cGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGCCGGCCGAAACCg                                                                              >  1:2205296/1‑101 (MQ=255)
               gAAGCGTGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGa                                                                             <  1:1168371/101‑1 (MQ=255)
               gAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGa                                                                             <  1:15755/101‑1 (MQ=255)
               gAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGa                                                                             <  2:614179/101‑1 (MQ=255)
                aaGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGAt                                                                            <  1:43158/101‑1 (MQ=255)
                aaGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGAt                                                                            >  2:1906405/1‑101 (MQ=255)
                 aGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGCCCGAAACCGATg                                                                           <  1:2299109/101‑1 (MQ=255)
                  gcgcgcGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATgg                                                                          >  2:3557853/1‑101 (MQ=255)
                   cgcgcgATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGgc                                                                         <  2:2117944/101‑1 (MQ=255)
                   cgcgcgATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGgc                                                                         <  2:2446895/101‑1 (MQ=255)
                    gcgcgATGCTGATCGACACGGTGACGCTGGTCAGTCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCGCGGCCCGGCCTGTCGGCCGAAACCGATGgcg                                                                        <  1:3675244/101‑1 (MQ=255)
                     cgcgATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATTgcgg                                                                       >  2:2822956/1‑100 (MQ=255)
                      gcgATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGcgcg                                                                      <  1:2706908/101‑1 (MQ=255)
                      gcgATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGcgcg                                                                      <  2:3210072/101‑1 (MQ=255)
                         aTGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGgc                                                                   >  1:2541188/1‑101 (MQ=255)
                         aTGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGgc                                                                   >  2:3709803/1‑101 (MQ=255)
                          tGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGgcg                                                                  <  2:1382157/101‑1 (MQ=255)
                           gCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGgcgc                                                                 <  1:131688/101‑1 (MQ=255)
                            cTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCt                                                                >  1:3324029/1‑101 (MQ=255)
                            cTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTCTCGGGCGAAACCGATGGCGCCGGCGCt                                                                >  2:3687178/1‑101 (MQ=255)
                             tGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTg                                                               <  1:2418545/101‑1 (MQ=255)
                             tGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTg                                                               >  1:741317/1‑101 (MQ=255)
                              gATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTgg                                                              >  2:2741295/1‑100 (MQ=255)
                               aTCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGGGCTGCa                                                             >  1:3980387/1‑101 (MQ=255)
                                tCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAg                                                            <  2:259102/101‑1 (MQ=255)
                                 cGACACGGCGACGCTGGTCAATCTGGTGTCTGATGCCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGCGCGCTGCAgg                                                           <  1:3655602/101‑1 (MQ=255)
                                   acacGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGAt                                                         <  2:2230348/101‑1 (MQ=255)
                                   acacGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGAt                                                         <  1:1554830/101‑1 (MQ=255)
                                   acacGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGAt                                                         <  1:3003689/101‑1 (MQ=255)
                                     acGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCt                                                       <  1:1748711/101‑1 (MQ=255)
                                     acGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCt                                                       <  1:3030249/101‑1 (MQ=255)
                                      cGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTg                                                      <  1:2221980/101‑1 (MQ=255)
                                       ggTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGt                                                     <  1:2794884/101‑1 (MQ=255)
                                       ggTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGt                                                     >  2:376809/1‑101 (MQ=255)
                                       ggTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGt                                                     <  2:4079668/101‑1 (MQ=255)
                                        gTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGGTCTGtt                                                    >  2:434849/1‑101 (MQ=255)
                                         tGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTg                                                   >  2:2051078/1‑101 (MQ=255)
                                          gACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGc                                                  >  2:1959580/1‑101 (MQ=255)
                                          gACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGc                                                  >  1:765790/1‑101 (MQ=255)
                                           aCGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCg                                                 >  2:3274964/1‑101 (MQ=255)
                                            cGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCgg                                                >  1:525701/1‑101 (MQ=255)
                                              cTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGa                                              >  2:1360425/1‑101 (MQ=255)
                                               tGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGAt                                             <  1:3257002/101‑1 (MQ=255)
                                                ggTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATc                                            >  2:2621726/1‑101 (MQ=255)
                                                ggTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATc                                            <  1:1297582/101‑1 (MQ=255)
                                                ggTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATc                                            >  1:3741111/1‑101 (MQ=255)
                                                 gTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCGGGATCTGTTGCGGGATcc                                           >  1:2852198/1‑101 (MQ=255)
                                                  tCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCg                                          <  2:395600/101‑1 (MQ=255)
                                                    aaTCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCTGCCGAAACCGATGGCGCGGGGGCTGCAGGAGCTGTTGCGGGATCCGCa                                        >  2:4203363/1‑101 (MQ=255)
                                                    aaTCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCa                                        <  2:740475/101‑1 (MQ=255)
                                                    aaTCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCa                                        <  2:299145/101‑1 (MQ=255)
                                                    aaTCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCACa                                        >  1:2276451/1‑101 (MQ=255)
                                                     aTCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCaa                                       <  1:2840060/101‑1 (MQ=255)
                                                     aTCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCaa                                       >  2:2533100/1‑101 (MQ=255)
                                                      tCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAc                                      <  2:4047582/101‑1 (MQ=255)
                                                       cTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGAGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAcg                                     <  1:1906525/101‑1 (MQ=255)
                                                        tGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAcgc                                    >  1:2674021/1‑101 (MQ=255)
                                                        tGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAcgc                                    >  1:1925408/1‑101 (MQ=255)
                                                          gtgtCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAcgccg                                  >  1:2531185/1‑101 (MQ=255)
                                                          gtgtCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAcgccg                                  <  1:1696090/101‑1 (MQ=255)
                                                          gtgtCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAcgccg                                  <  2:1007133/101‑1 (MQ=255)
                                                           tgtCTGAGACCCGGGCGGTGCCGGAATTCCTCGTCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCannnnnnn                                 >  1:1411633/1‑94 (MQ=255)
                                                           tgtCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAAcgccgc                                 <  2:694828/101‑1 (MQ=255)
                                                             tCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCcgcgc                               <  2:860382/101‑1 (MQ=255)
                                                              cTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCcgcgcg                              <  1:1022736/101‑1 (MQ=255)
                                                                gagaCCCGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCgcggc                            >  2:3456114/1‑101 (MQ=255)
                                                                    cccGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAg                        <  2:2673815/101‑1 (MQ=255)
                                                                    cccGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAg                        <  1:4029561/101‑1 (MQ=255)
                                                                    cccGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAg                        <  2:1347847/101‑1 (MQ=255)
                                                                      cGGGCGGTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGt                      >  1:1021601/1‑101 (MQ=255)
                                                                           ggTGCCGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCg                 >  2:2164802/1‑101 (MQ=255)
                                                                               ccGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGTGCGACGCAGGATCTGTTGCGGGGTCGGCAACGCCGCCCGGCGCAGGCTTCCGCCAt             >  2:1824320/1‑101 (MQ=255)
                                                                                cGGAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATg            >  1:3481005/1‑101 (MQ=255)
                                                                                 ggAATTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATgg           <  1:2741501/101‑1 (MQ=255)
                                                                                   aaTTCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGaa         <  2:2113434/101‑1 (MQ=255)
                                                                                     ttCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAac       >  1:2345117/1‑101 (MQ=255)
                                                                                      tCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAacg      >  2:3737190/1‑101 (MQ=255)
                                                                                      tCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAacg      >  1:1685278/1‑101 (MQ=255)
                                                                                      tCCTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAacg      <  2:3384311/101‑1 (MQ=255)
                                                                                        cTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAacgac    <  2:3740857/101‑1 (MQ=255)
                                                                                        cTCGGCCCGGCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAacgac    >  1:500831/1‑101 (MQ=255)
                                                                                          cggcccggCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAAcgacga  <  1:2751461/101‑1 (MQ=255)
                                                                                          cggcccggCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAAcgacga  >  1:2391105/1‑101 (MQ=255)
                                                                                          cggcccggCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAAcgacga  <  1:2072461/101‑1 (MQ=255)
                                                                                          cggcccggCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAAcgacga  >  2:2262230/1‑101 (MQ=255)
                                                                                                 |                                                                                             
GACGTGGCATATCACGAAGCGCGCGATGCTGATCGACACGGTGACGCTGGTCAATCTGGTGTCTGAGACCCGGGCGGTGCCGGAATTCCTCGGCCGGCCCTGTCGGCCGAAACCGATGGCGCGGGCGCTGCAGGATCTGTTGCGGGATCCGCAACGCCGCGCGGCGCAGGTTTCCGCCATGGAAACGACGA  >  NC_014034/1770550‑1770740

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG