Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,782,185 C→T 79.3% S661L (TCA→ATA)  RCAP_rcc01644 → FMN‑binding domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,782,1850C→T79.3% 95.2 ‑1.1 0.0164S661L (TCA→TTA) RCAP_rcc01644FMN‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (60/70):  ref base (19/15):  total (79/85)
Fisher's exact test strand distribution p-value = 3.40e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 167 total aligned reads displayed.

TGTGCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.TCACTTGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCG.AACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTGG  >  NC_014034/1782095‑1782275
                                                                                           |                                                                                           
tgtgCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATgacgac                                                                                   <  2:3965123/101‑1 (MQ=255)
 gtgCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACa                                                                                  >  2:4178325/1‑101 (MQ=255)
 gagCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACa                                                                                  <  1:986833/99‑1 (MQ=255)
  tgCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACaa                                                                                 <  2:128485/101‑1 (MQ=255)
    ccGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACTACaaaa                                                                               <  2:230250/101‑1 (MQ=255)
    ccGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACaaaa                                                                               <  2:1710764/101‑1 (MQ=255)
     cgcgTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAg                                                                              <  1:514863/101‑1 (MQ=255)
      gcgTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGc                                                                             <  1:1261336/101‑1 (MQ=255)
      gcgTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGc                                                                             >  2:1440223/1‑101 (MQ=255)
       cgTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGcc                                                                            <  1:2571750/101‑1 (MQ=255)
       cgTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGcc                                                                            <  1:1656923/101‑1 (MQ=255)
        gTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTGCAGTGCCTTGNCTGTGTGGCG.ATCCATGACGACAAAAGCCg                                                                           >  1:787957/1‑101 (MQ=255)
        gTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCg                                                                           <  1:311076/101‑1 (MQ=255)
        gTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCg                                                                           <  1:2178069/101‑1 (MQ=255)
        gTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCg                                                                           >  1:1782424/1‑101 (MQ=255)
          gcgctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCt                                                                         <  1:4101798/101‑1 (MQ=255)
           cgctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCtg                                                                        <  1:1711077/101‑1 (MQ=255)
            gctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCtgt                                                                       >  2:3024996/1‑101 (MQ=255)
             ctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCtgtg                                                                      <  2:1581118/101‑1 (MQ=255)
             ctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCtgtg                                                                      >  2:3324914/1‑101 (MQ=255)
              tgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCtgtgt                                                                     >  2:1319640/1‑101 (MQ=255)
               gcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.CTCCATGACGACAAAAGCGGCtgtgtg                                                                    >  2:2109430/1‑101 (MQ=255)
                 gcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGcc                                                                  >  2:258682/1‑101 (MQ=255)
                  ccTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCt                                                                 >  1:3074585/1‑101 (MQ=255)
                  ccTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCt                                                                 >  2:2133298/1‑101 (MQ=255)
                   ccaTGTCGCGATAAAGCGCAACGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCtt                                                                <  2:2697579/99‑1 (MQ=255)
                   cTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCtt                                                                >  2:1242021/1‑101 (MQ=255)
                    tATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCttt                                                               <  1:1795337/101‑1 (MQ=255)
                    tATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCttt                                                               >  2:837470/1‑101 (MQ=255)
                       ggCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGt                                                            >  2:1062385/1‑101 (MQ=255)
                        gcgcGATCAAGCGCACCGGCGAGATCCGCTATTTCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTg                                                           <  1:3610910/101‑1 (MQ=255)
                         cgcgATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGc                                                          <  1:709577/101‑1 (MQ=255)
                          gcgATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCt                                                         >  2:1193368/1‑101 (MQ=255)
                            gATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTgg                                                       <  2:3711541/101‑1 (MQ=255)
                              tCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggcg                                                     >  1:653070/1‑101 (MQ=255)
                              tCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggcg                                                     <  1:822805/101‑1 (MQ=255)
                               cAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggcgg                                                    >  1:977533/1‑101 (MQ=255)
                               cAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCCGTGTGCCTTTGGTGCTggcgg                                                    >  1:118626/1‑101 (MQ=255)
                                aaGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggcggc                                                   <  2:1782305/101‑1 (MQ=255)
                                 aGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGgcggcg                                                  >  2:1844964/1‑101 (MQ=255)
                                  gcgcACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.a                                                <  1:3487027/101‑1 (MQ=255)
                                    gcACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaa                                              <  2:3243896/101‑2 (MQ=255)
                                    gcACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaa                                              >  1:2774739/1‑100 (MQ=255)
                                    gcACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaa                                              <  2:2036973/101‑2 (MQ=255)
                                     cACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaac                                             <  2:1021225/101‑3 (MQ=255)
                                     cACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaac                                             >  1:294196/1‑99 (MQ=255)
                                     cACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaac                                             <  2:2671760/101‑3 (MQ=255)
                                      cccGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaacg                                            <  2:2560674/100‑4 (MQ=255)
                                        tctcGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaacgaa                                          <  1:3708291/98‑6 (MQ=255)
                                        cGGAGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaacgaa                                          <  1:1403233/101‑6 (MQ=255)
                                         ggCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGaaa                                          >  1:2555887/1‑101 (MQ=255)
                                           cGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAga                                        >  2:3708037/1‑100 (MQ=255)
                                           cGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAgg                                        >  2:3120112/1‑101 (MQ=255)
                                             agaTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCt                                      <  2:1211809/101‑1 (MQ=255)
                                             agaTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCt                                      <  1:3325155/101‑1 (MQ=255)
                                               aTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCg                                    <  2:2857731/101‑1 (MQ=255)
                                               aTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCg                                    <  2:439963/101‑1 (MQ=255)
                                                tCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCgg                                   >  2:1942955/1‑101 (MQ=255)
                                                 ccGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCggg                                  <  1:76625/101‑1 (MQ=255)
                                                  cGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGc                                 >  2:3015798/1‑101 (MQ=255)
                                                  cGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGc                                 <  1:70310/101‑1 (MQ=255)
                                                  cGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGc                                 <  2:4117397/101‑1 (MQ=255)
                                                   gCTATTCCGAGTGTTTCCAGTGCCTTGACTGTATGGCG.ATCCATGAAGACAAAAGCCGCTGTGTGCCTTTGGTGCGGGCGGGGAAACGACAGGCTCGGACa                                >  1:194899/1‑101 (MQ=255)
                                                    cTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCAt                               >  2:2150364/1‑101 (MQ=255)
                                                    cTATTCCGAGTGTTTCCAGTGCCTTGACGGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCAt                               <  1:2108107/101‑1 (MQ=255)
                                                     tATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATg                              >  1:905870/1‑101 (MQ=255)
                                                      aTTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGa                             >  2:3933256/1‑101 (MQ=255)
                                                      aTTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGa                             >  2:2183904/1‑101 (MQ=255)
                                                       ttCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGaa                            <  2:977442/101‑1 (MQ=255)
                                                       ttCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGaa                            <  1:2133443/101‑1 (MQ=255)
                                                        tCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAg                           >  2:3407022/1‑101 (MQ=255)
                                                        tCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAg                           <  2:2509882/101‑1 (MQ=255)
                                                        tCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAg                           <  1:1104939/101‑1 (MQ=255)
                                                         ccGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGCTGCTGGCGGCGAAAGGAAAGGCTCCGGCATGGAgc                          <  2:397712/101‑1 (MQ=255)
                                                          cGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcg                         >  2:1103824/1‑101 (MQ=255)
                                                          cGAGTGTTTCCAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcg                         >  1:3302716/1‑101 (MQ=255)
                                                           gAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcgg                        <  2:292223/101‑1 (MQ=255)
                                                           gAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcgg                        >  1:3912646/1‑101 (MQ=255)
                                                           gAGTGTTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATAAAgcgg                        >  1:672986/1‑101 (MQ=255)
                                                             gtgtTTCCAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggcg                      >  2:229773/1‑101 (MQ=255)
                                                                   ccAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGcggcggcgggt                >  2:590844/1‑99 (MQ=255)
                                                                   ccAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGtt                <  1:10780/101‑1 (MQ=255)
                                                                   ccAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGtt                >  2:1814613/1‑101 (MQ=255)
                                                                   caaGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGtt                <  2:4216166/99‑1 (MQ=255)
                                                                    cAGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGttt               >  1:1285259/1‑101 (MQ=255)
                                                                    cAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGttt               >  2:1637600/1‑101 (MQ=255)
                                                                    cAGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGgtt               >  1:311223/1‑101 (MQ=255)
                                                                    cAGTGCCTTCACGGTGTGCCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGttt               <  2:2781913/101‑1 (MQ=255)
                                                                     aGTGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTc              >  1:2437034/1‑101 (MQ=255)
                                                                     aGTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTc              <  2:602911/101‑1 (MQ=255)
                                                                      gTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGttttt             >  1:1559582/1‑99 (MQ=255)
                                                                      gTGCCTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCt             <  1:331015/101‑1 (MQ=255)
                                                                       tGCCTTGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTg            >  1:1491275/1‑101 (MQ=255)
                                                                         ccTTGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCa          >  1:261851/1‑101 (MQ=255)
                                                                         ccTGGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCa          <  2:2546022/101‑1 (MQ=255)
                                                                           ttGACTGTGTGGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGa        >  2:3058530/1‑101 (MQ=255)
                                                                           ttGACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGa        <  2:1053940/101‑1 (MQ=255)
                                                                             gACTGTGTGGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATc      >  1:66112/1‑101 (MQ=255)
                                                                             gACTGTGTGGCG.ATCCATGACGACAAAAGCCGCGGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATc      <  1:3642522/101‑1 (MQ=255)
                                                                                 gtgtgGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTgg  <  1:2018727/101‑1 (MQ=255)
                                                                                           |                                                                                           
TGTGCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGTGGCG.TCACTTGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCG.AACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTGG  >  NC_014034/1782095‑1782275

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG