Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,965,239	G→T	100%	intergenic (+7/+85)	bdhA → / ← RCAP_rcc01818	3‑hydroxybutyrate dehydrogenase/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,965,239	0	G→T	100.0%	199.8	52	intergenic (+7/+85)	bdhA/RCAP_rcc01818	3‑hydroxybutyrate dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (26/26):  ref base (0/0):  total (26/26)

TGGGGGGAACGACGGTCTTCCTGTGTGCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTGC.GCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GCGGGCTCCG.CCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGG  >  NC_014034/1965141‑1965331                                                                                                   |                                                                                                  tGGGGGGAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTtc.g                                                                                                >  1:1755448/1‑101 (MQ=255)   gggggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.Gcc                                                                                              <  1:3484083/101‑1 (MQ=255)   gggggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.Gcc                                                                                              <  1:1770046/101‑1 (MQ=255)   gggggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCAACGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.Gcc                                                                                              <  2:1188543/101‑1 (MQ=255)    ggggAACGACGGTCTTGCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCt                                                                                             <  1:3049626/101‑1 (MQ=255)     gggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTACTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTc                                                                                            <  2:1640298/101‑1 (MQ=255)      ggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCg                                                                                           <  1:3259111/101‑1 (MQ=255)       gAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGGGATTTC.GCCTCgg                                                                                          >  1:1811752/1‑101 (MQ=255)       gAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCgg                                                                                          >  2:1403962/1‑101 (MQ=255)        aaCGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCggg                                                                                         >  2:2517356/1‑101 (MQ=255)        aaCGACGGTCTTCCTGTGTTCCGAGGCGGCGGCACAGCTCACCGGCACGACGATTTCCGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCggg                                                                                         <  1:3620897/101‑1 (MQ=255)         acgacgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGACTGGACGGCGCTGTGAGAGATTTC.GCCTCgggg                                                                                        <  2:1345843/101‑1 (MQ=255)          cgacgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGc                                                                                       <  2:339718/101‑1 (MQ=255)          cgacgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGc                                                                                       <  2:646786/101‑1 (MQ=255)          cgacgGTCTTCCTGGGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGc                                                                                       <  1:1143080/101‑1 (MQ=255)            acgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCaa                                                                                     <  1:1618539/101‑1 (MQ=255)             cgGTCTTCCTGTGTTCCGAGTCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAg                                                                                    <  2:165525/101‑1 (MQ=255)             cgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAg                                                                                    <  1:4147023/101‑1 (MQ=255)             cgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAg                                                                                    >  2:3550912/1‑101 (MQ=255)             cgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAg                                                                                    >  1:2851029/1‑101 (MQ=255)               gTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGcc                                                                                  <  1:3119950/101‑1 (MQ=255)               gTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGcc                                                                                  <  2:2726238/101‑1 (MQ=255)                tCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGccc                                                                                 <  1:1817823/101‑1 (MQ=255)                tCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGccc                                                                                 <  1:403537/101‑1 (MQ=255)                  ttCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGccccg                                                                               >  2:1175507/1‑99 (MQ=255)                    ccTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAgg                                                                             >  2:156166/1‑101 (MQ=255)                     cTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGc                                                                            >  1:1462572/1‑101 (MQ=255)                     cTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGc                                                                            >  1:4087429/1‑101 (MQ=255)                     cTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGc                                                                            <  2:3351521/101‑1 (MQ=255)                     cTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGc                                                                            <  1:2596295/101‑1 (MQ=255)                      tgtgttCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCg                                                                           >  1:459898/1‑101 (MQ=255)                       gtgttCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCCGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGa                                                                          <  1:2137129/101‑1 (MQ=255)                        tgttCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGAc                                                                         <  1:1515687/101‑1 (MQ=255)                        tgttCCGAGACGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGAc                                                                         <  2:314056/101‑1 (MQ=255)                         gttccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGAcc                                                                        >  2:2519229/4‑101 (MQ=255)                         gttccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGAcc                                                                        <  1:2323657/98‑1 (MQ=255)                          ttccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGGGAccc                                                                       >  2:2796734/3‑101 (MQ=255)                          ttccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGAccc                                                                       <  2:2446319/99‑1 (MQ=255)                           tccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGACccg                                                                      >  1:2144614/2‑101 (MQ=255)                           tccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGACccg                                                                      <  1:1424945/100‑1 (MQ=255)                            ccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGACccg.c                                                                    >  2:4199541/1‑101 (MQ=255)                             cGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGACccg.cc                                                                   >  1:2182530/1‑101 (MQ=255)                              gAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGACccg.cc..g                                                                >  1:341791/1‑101 (MQ=255)                              gAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGACccg.cc..g                                                                >  2:1339096/1‑101 (MQ=255)                               aGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGACccg.cccc                                                                 >  1:2134807/1‑99 (MQ=255)                                  cggcggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGACccg.cccgggg                                                              >  1:1951744/1‑96 (MQ=255)                                    gcggcgCAGATCAGCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGACCCG.CC...CGGGcgg                                                         >  2:907212/1‑99 (MQ=39)                                     cggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGACccg.cccggggggg                                                           >  1:1186698/1‑93 (MQ=255)                                      ggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGCTcc                                                        >  2:1274787/1‑101 (MQ=255)                                         gcAGATCACCGGCACGACGCTTTCTGTGGATGGCGGCTGGACGGCGGGGTGAGAGATTTA.GCCTCGGGGCAAGCCCGAGGCAGCCCG.GC..GGGGGcctgccc                                                     >  2:1319465/1‑95 (MQ=255)                                         gcAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GCggggggncgc                                                     >  1:3462983/1‑94 (MQ=255)                                           aGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGGGGCG.cccc                                                  >  1:2705529/1‑101 (MQ=38)                                                 ccGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGACCCG.CC..CCGGGGGGCG.ccccccgcgc                                            >  1:1178794/1‑95 (MQ=37)                                                          cgaTTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGACCCGCCC..GGGGGTTCCC.CCCCCGGccccccggggc                                    >  2:1218062/1‑95 (MQ=25)                                                               tCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGCTCCGCCCCCCGGCCCCCCAGGGATTTCa                               >  2:2595796/1‑101 (MQ=21)                                                                       ggggggcGGGGCGGCGCTGGGAG.TATTGCGGGCGCGGGCCAGGCCCGAGGGCCCCCG.CC..GGGGGCTCCGCCCCCGGACCCCCGAGGTATTTCAGGCAAGAt                       <  1:559281/97‑1 (MQ=2)                                                                                gccGGCGCTGGGAGATTTCGC.CCCGGGGGGCACGCCCGAGCCCCCCCG.CC..GGGGGCTCCGCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAggg              <  2:459841/99‑1 (MQ=9)                                                                                      gttgtgAGAGTTTCC.GCCGCGGCGCACGCCCGGGCCCCCCCG.CC..GGGGGCTCCGCCCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTc        <  2:2705323/99‑1 (MQ=16)                                                                                            agagaTTTC.GCCTCGGGGCAAGCCCGAGGCGACCCG.CCGGGGGGGCCCCC.CCCCGGCCCCCCCGGGGGTTTTGGGCAGAAGAAAAGAGGGGGGC.CAggcgg  >  2:2464724/1‑101 (MQ=2)                                                                                                   |                                                                                                  TGGGGGGAACGACGGTCTTCCTGTGTGCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTGC.GCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GCGGGCTCCG.CCCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGG  >  NC_014034/1965141‑1965331 Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG