Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,976,892 G→C 89.4% intergenic (‑71/+9) fba ← / ← gap1 fructose‑bisphosphate aldolase/glyceraldehyde‑3‑phosphate dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,976,8920G→C89.4% 29.5 ‑0.5 ‑0.3155intergenic (‑71/+9)fba/gap1fructose‑bisphosphate aldolase/glyceraldehyde‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  new base (93/44):  ref base (10/7):  total (103/52)
Fisher's exact test strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.51e-01

Only 100 of 166 total aligned reads displayed.

CGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCGGCG..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAT  >  NC_014034/1976802‑1976990
                                                                                              |                                                                                                    
cGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTtctc                                                                                          >  2:1126523/1‑101 (MQ=255)
 gAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCa                                                                                         >  1:1130741/1‑101 (MQ=255)
 gAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCa                                                                                         >  1:1296653/1‑101 (MQ=255)
  aaGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAg                                                                                        >  2:1194313/1‑101 (MQ=255)
   aGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGa                                                                                       >  1:609352/1‑101 (MQ=255)
   aGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGa                                                                                       >  2:1492652/1‑101 (MQ=255)
   aGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGa                                                                                       >  2:1484523/1‑101 (MQ=255)
      gTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCa                                                                                    >  2:2889912/1‑101 (MQ=255)
      gTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCa                                                                                    >  2:1724193/1‑101 (MQ=255)
       tGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAg                                                                                   >  1:1773532/1‑101 (MQ=255)
           cAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTg                                                                               >  2:2443667/1‑101 (MQ=255)
           cAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTg                                                                               >  2:362646/1‑101 (MQ=255)
           cAGTCCCTTTTTCTCATTTCTTGGGGTTCGCGGCTTTTTTCTGGG.CCA.AAATCGGC.GGGGGGCCGGGGGG.CACCCCCCCCGGC..TTTTCTCAGATCAGCTTg                                                                               <  1:2804365/101‑1 (MQ=255)
            aGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCttcc                                                                              >  1:477304/1‑99 (MQ=255)
             tgccttttttCCAAGTTCCTTGGTTTCG.GGCCTTTTTTTTGGCCCA.AAATGCCC.CGGGGCCCGGGGGG.CGGCCCCCCGGGC..TTTTCTCAGATCAGCTTGcc                                                                             <  2:3124346/95‑1 (MQ=255)
              tGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGccc                                                                            >  1:1525926/1‑101 (MQ=255)
               gCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCa                                                                           >  1:2628957/1‑101 (MQ=255)
                cttttttCTCGTTTCTTTGTTTTCGGGCCTTTTTTCTTGC.CAA.AAACCCGC.GGGGGGCCGGGGGG.CGCCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCAt                                                                          <  1:1177583/98‑1 (MQ=255)
                cctttttCTCGTTTCCTGGAGTTGGGGGCCTTTTTCTTGC.CCG.AAATCCGC.CGGGGGCCGGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCAt                                                                          <  2:3454024/98‑1 (MQ=16)
                cctttttCTCAGTTCTTTGGGTTCGCGGCTTTTTTCTGGG.CCA.AAATCCGC.GGGGGGCCGGGGGG.CAGCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCAt                                                                          <  1:1954573/98‑1 (MQ=255)
                  aTTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCg                                                                        >  2:2274646/1‑101 (MQ=255)
                   ttttCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGc                                                                       >  2:718483/1‑101 (MQ=255)
                    tttCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGcc                                                                      >  1:4141890/1‑101 (MQ=255)
                    tttCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGcc                                                                      >  2:3700897/1‑101 (MQ=255)
                     ttCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCa                                                                     >  2:820777/1‑101 (MQ=255)
                      tctcAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCAc                                                                    <  2:3476157/101‑1 (MQ=255)
                      tctcAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCAc                                                                    >  2:2324908/1‑101 (MQ=255)
                      tcccAGTTCTTTGATTCCGCGTCTTTTTTCTGGC.CCA.AAACACGC.GGGGGGCCGGGGGG.CGGCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCAc                                                                    <  1:2400782/98‑1 (MQ=255)
                       ctcAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCAcg                                                                   >  2:3404870/1‑101 (MQ=255)
                           gTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGGCCGGGGG.CGGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCAcggcgg                                                               >  1:3191694/1‑101 (MQ=38)
                            ttCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATGAGCTTGCCCATCGCCACGGCGGt                                                              >  2:2066983/1‑101 (MQ=37)
                             tCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTa                                                             >  2:3235310/1‑101 (MQ=255)
                             tCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACAC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTa                                                             >  2:380924/1‑101 (MQ=37)
                             tCCCTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTa                                                             >  1:717517/1‑101 (MQ=37)
                              tctttggTTCGGCGCCTTTTTTCTGG.CCA.AAAATCCG.CGGGGGGCCGGGGG.CCGCCCCCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTa                                                             <  1:3947084/95‑1 (MQ=9)
                                ttGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCg                                                          >  2:2198652/1‑101 (MQ=255)
                                 tGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCgg                                                         >  1:3248702/1‑101 (MQ=255)
                                 tGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCgg                                                         >  1:3550076/1‑101 (MQ=255)
                                 tGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCgg                                                         >  2:2817640/1‑101 (MQ=255)
                                  gAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGc                                                        >  1:3450388/1‑101 (MQ=255)
                                    gTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCa                                                      >  2:4017286/1‑101 (MQ=255)
                                     ttCGCGGCTTTTTTCTGGG.CCG.AAATCCGC.GGGGGGCCGGGGGG.CAGCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCAt                                                     <  1:2706977/101‑1 (MQ=25)
                                        gcgGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCg                                                  >  1:3457480/1‑101 (MQ=255)
                                        gcgGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.AAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCg                                                  <  1:2252787/101‑1 (MQ=37)
                                        cgggCCTTTTTTCTCG.CCGAAAATCCGC.GGGGGGCCGGGGGG.CACCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGc                                                   <  1:4130989/99‑1 (MQ=12)
                                          ggCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGt                                                >  2:870795/1‑101 (MQ=255)
                                             cTTTTTTCTGG.CCGAAAACCCGC.GGGGGGTCGGGGGG.AACCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTc                                              <  2:3338500/101‑1 (MQ=12)
                                             cTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCg                                             >  2:2244363/1‑101 (MQ=255)
                                              tttttCTTGGCCCA.AAATCCGC.GGGGGGCCGGGGGC.CACCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCg                                             <  1:3978836/101‑1 (MQ=12)
                                              tttttCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCgg                                            >  1:590132/1‑100 (MQ=255)
                                              tttttCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCga                                            >  1:1087632/1‑101 (MQ=255)
                                              cttttTTTGG.GCC.AAAAACGCGGGGGGGGCCGGGGGCCCCCCCCCCGGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCgaaat                                              <  2:2132310/100‑5 (MQ=9)
                                                tttCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCgaga                                          >  2:2660467/1‑101 (MQ=255)
                                                ttgcTTGG.CCG.AAATGCGC.CGGGGGCCGGGGGG.CAGCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCgaga                                          <  2:3457228/98‑1 (MQ=21)
                                                 ttCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGaa                                         >  2:846195/1‑101 (MQ=255)
                                                  tCTTGG.CCG.AAATGCGC.GGGGGGTCCGGGGG.CAGCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAg                                        <  2:3133100/101‑1 (MQ=25)
                                                  tCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAg                                        >  2:2841957/1‑101 (MQ=255)
                                                   cTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTGTCGGCCATGCGGTTCGAGAAGc                                       >  1:3936636/1‑101 (MQ=25)
                                                    ttGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGGGAAGcc                                      >  2:2743630/1‑101 (MQ=25)
                                                    ttGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGcc                                      >  2:3335793/1‑101 (MQ=255)
                                                    ttGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGcc                                      <  2:609288/101‑1 (MQ=255)
                                                     tGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGccc                                     >  1:1510700/1‑101 (MQ=255)
                                                      gg.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGcccc                                    <  2:3325523/101‑1 (MQ=255)
                                                      gg.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGcccc                                    >  2:1302425/1‑101 (MQ=255)
                                                         ccG.AAATCCGC.GGGGGGTCGGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcat                                  <  2:241165/101‑1 (MQ=21)
                                                         ccG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGGCCcat                                  >  2:3007974/1‑101 (MQ=25)
                                                         ccG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcat                                  <  2:2800390/101‑1 (MQ=255)
                                                           g.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattc                                >  1:1883583/1‑101 (MQ=255)
                                                             aaaTCCGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattca                               <  2:590068/101‑1 (MQ=21)
                                                             aaaTACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattca                               >  1:2444810/1‑101 (MQ=255)
                                                             aaaTACGC.CGGGGGTCCGGGGG.CAGCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattca                               <  2:4088520/101‑1 (MQ=21)
                                                               atccGC.GGGGGAACCGGTGG.AGGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcatt                             <  1:1384387/98‑1 (MQ=17)
                                                                atcgcgggggggCCGGGGGG.CGCCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcatt                             <  1:2488191/94‑1 (MQ=17)
                                                                 ccgatctggggTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTg                            <  2:3900662/94‑1 (MQ=21)
                                                                 aCGC.GGGGGGGGCGGGGG.CGCCGCCCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcatt                             <  1:3052168/101‑1 (MQ=17)
                                                                    ggggggggTGCGGGGC.GCGCCCCCCGGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCa                         <  1:1048961/98‑1 (MQ=17)
                                                                    c.cGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCAt                        >  2:3631603/1‑101 (MQ=255)
                                                                      cGGGGGTCCGGGGG.CGCCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATa                       <  1:3393931/101‑1 (MQ=18)
                                                                      cGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATa                       >  2:3190725/1‑101 (MQ=255)
                                                                        ggggTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTgagatcgg                     >  1:3900921/1‑94 (MQ=18)
                                                                           gcccGGGGG.CCCCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACg                  <  2:1088491/99‑1 (MQ=18)
                                                                           gTCCGGGGG.CAGCGCCCCCTGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACg                  <  2:2047496/101‑1 (MQ=18)
                                                                             cgggggg.CCGCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGAc                <  1:1126617/99‑1 (MQ=18)
                                                                             ccGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGAc                >  2:2731670/1‑101 (MQ=255)
                                                                              ggggag.CGGCGCCCGGGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACa               <  1:2842176/100‑1 (MQ=18)
                                                                              cGGGGG.GAGCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACa               <  1:718559/101‑1 (MQ=18)
                                                                              cGGGGG.CAGCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACa               <  2:3248461/101‑1 (MQ=21)
                                                                               ggggg.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAg              >  2:3830921/1‑101 (MQ=255)
                                                                                 ggg.CACCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGa            <  2:2016499/101‑1 (MQ=18)
                                                                                  tg.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGAt           >  2:2980874/2‑101 (MQ=18)
                                                                                   gcgccccccccGGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGAt           <  2:1510507/94‑1 (MQ=18)
                                                                                   g.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATg          >  1:667676/1‑101 (MQ=255)
                                                                                   g.CAGCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATg          <  1:1290345/101‑1 (MQ=18)
                                                                                    ccccccccccGGC...TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGc         <  1:3701151/95‑1 (MQ=255)
                                                                                     gaGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGc         <  2:544884/100‑1 (MQ=255)
                                                                                      aGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATgtg        >  2:1870827/1‑99 (MQ=255)
                                                                                      aGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCg        <  2:213112/101‑1 (MQ=255)
                                                                                      aGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCg        <  1:3831178/101‑1 (MQ=255)
                                                                                        gcccccGGC...TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAc     >  1:2063886/3‑101 (MQ=255)
                                                                                          ccccGGC...TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCa   >  2:283710/1‑101 (MQ=255)
                                                                                              |                                                                                                    
CGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCGGCG..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAT  >  NC_014034/1976802‑1976990

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG