Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_014034 | 1,976,892 | G→C | 89.4% | intergenic (‑71/+9) | fba ← / ← gap1 | fructose‑bisphosphate aldolase/glyceraldehyde‑3‑phosphate dehydrogenase |
Read alignment evidence... | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | position | change | freq | score | reads | annotation | genes | product | ||
* | NC_014034 | 1,976,892 | 0 | G→C | 89.4% | 29.5 ‑0.5 ‑0.3 | 155 | intergenic (‑71/+9) | fba/gap1 | fructose‑bisphosphate aldolase/glyceraldehyde‑3‑phosphate dehydrogenase |
Reads supporting (aligned to +/- strand): new base (93/44): ref base (10/7): total (103/52) | ||||||||||
Fisher's exact test strand distribution p-value = 5.85e-01 | ||||||||||
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.51e-01 |
Only 100 of 166 total aligned reads displayed.
CGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCGGCG..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAT > NC_014034/1976802‑1976990
|
cGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTtctc > 2:1126523/1‑101 (MQ=255)
gAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCa > 1:1130741/1‑101 (MQ=255)
gAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCa > 1:1296653/1‑101 (MQ=255)
aaGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAg > 2:1194313/1‑101 (MQ=255)
aGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGa > 1:609352/1‑101 (MQ=255)
aGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGa > 2:1492652/1‑101 (MQ=255)
aGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGa > 2:1484523/1‑101 (MQ=255)
gTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCa > 2:2889912/1‑101 (MQ=255)
gTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCa > 2:1724193/1‑101 (MQ=255)
tGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAg > 1:1773532/1‑101 (MQ=255)
cAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTg > 2:2443667/1‑101 (MQ=255)
cAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTg > 2:362646/1‑101 (MQ=255)
cAGTCCCTTTTTCTCATTTCTTGGGGTTCGCGGCTTTTTTCTGGG.CCA.AAATCGGC.GGGGGGCCGGGGGG.CACCCCCCCCGGC..TTTTCTCAGATCAGCTTg < 1:2804365/101‑1 (MQ=255)
aGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCttcc > 1:477304/1‑99 (MQ=255)
tgccttttttCCAAGTTCCTTGGTTTCG.GGCCTTTTTTTTGGCCCA.AAATGCCC.CGGGGCCCGGGGGG.CGGCCCCCCGGGC..TTTTCTCAGATCAGCTTGcc < 2:3124346/95‑1 (MQ=255)
tGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGccc > 1:1525926/1‑101 (MQ=255)
gCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCa > 1:2628957/1‑101 (MQ=255)
cttttttCTCGTTTCTTTGTTTTCGGGCCTTTTTTCTTGC.CAA.AAACCCGC.GGGGGGCCGGGGGG.CGCCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCAt < 1:1177583/98‑1 (MQ=255)
cctttttCTCGTTTCCTGGAGTTGGGGGCCTTTTTCTTGC.CCG.AAATCCGC.CGGGGGCCGGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCAt < 2:3454024/98‑1 (MQ=16)
cctttttCTCAGTTCTTTGGGTTCGCGGCTTTTTTCTGGG.CCA.AAATCCGC.GGGGGGCCGGGGGG.CAGCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCAt < 1:1954573/98‑1 (MQ=255)
aTTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCg > 2:2274646/1‑101 (MQ=255)
ttttCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGc > 2:718483/1‑101 (MQ=255)
tttCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGcc > 1:4141890/1‑101 (MQ=255)
tttCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGcc > 2:3700897/1‑101 (MQ=255)
ttCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCa > 2:820777/1‑101 (MQ=255)
tctcAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCAc < 2:3476157/101‑1 (MQ=255)
tctcAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCAc > 2:2324908/1‑101 (MQ=255)
tcccAGTTCTTTGATTCCGCGTCTTTTTTCTGGC.CCA.AAACACGC.GGGGGGCCGGGGGG.CGGCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCAc < 1:2400782/98‑1 (MQ=255)
ctcAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCAcg > 2:3404870/1‑101 (MQ=255)
gTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGGCCGGGGG.CGGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCAcggcgg > 1:3191694/1‑101 (MQ=38)
ttCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATGAGCTTGCCCATCGCCACGGCGGt > 2:2066983/1‑101 (MQ=37)
tCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTa > 2:3235310/1‑101 (MQ=255)
tCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACAC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTa > 2:380924/1‑101 (MQ=37)
tCCCTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTa > 1:717517/1‑101 (MQ=37)
tctttggTTCGGCGCCTTTTTTCTGG.CCA.AAAATCCG.CGGGGGGCCGGGGG.CCGCCCCCCCCGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTa < 1:3947084/95‑1 (MQ=9)
ttGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCg > 2:2198652/1‑101 (MQ=255)
tGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCgg > 1:3248702/1‑101 (MQ=255)
tGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCgg > 1:3550076/1‑101 (MQ=255)
tGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCgg > 2:2817640/1‑101 (MQ=255)
gAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGc > 1:3450388/1‑101 (MQ=255)
gTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCa > 2:4017286/1‑101 (MQ=255)
ttCGCGGCTTTTTTCTGGG.CCG.AAATCCGC.GGGGGGCCGGGGGG.CAGCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCAt < 1:2706977/101‑1 (MQ=25)
gcgGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCg > 1:3457480/1‑101 (MQ=255)
gcgGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.AAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCg < 1:2252787/101‑1 (MQ=37)
cgggCCTTTTTTCTCG.CCGAAAATCCGC.GGGGGGCCGGGGGG.CACCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGc < 1:4130989/99‑1 (MQ=12)
ggCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGt > 2:870795/1‑101 (MQ=255)
cTTTTTTCTGG.CCGAAAACCCGC.GGGGGGTCGGGGGG.AACCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTc < 2:3338500/101‑1 (MQ=12)
cTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCg > 2:2244363/1‑101 (MQ=255)
tttttCTTGGCCCA.AAATCCGC.GGGGGGCCGGGGGC.CACCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCg < 1:3978836/101‑1 (MQ=12)
tttttCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCgg > 1:590132/1‑100 (MQ=255)
tttttCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCga > 1:1087632/1‑101 (MQ=255)
cttttTTTGG.GCC.AAAAACGCGGGGGGGGCCGGGGGCCCCCCCCCCGGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCgaaat < 2:2132310/100‑5 (MQ=9)
tttCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCgaga > 2:2660467/1‑101 (MQ=255)
ttgcTTGG.CCG.AAATGCGC.CGGGGGCCGGGGGG.CAGCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCgaga < 2:3457228/98‑1 (MQ=21)
ttCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGaa > 2:846195/1‑101 (MQ=255)
tCTTGG.CCG.AAATGCGC.GGGGGGTCCGGGGG.CAGCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAg < 2:3133100/101‑1 (MQ=25)
tCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAg > 2:2841957/1‑101 (MQ=255)
cTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTGTCGGCCATGCGGTTCGAGAAGc > 1:3936636/1‑101 (MQ=25)
ttGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGGGAAGcc > 2:2743630/1‑101 (MQ=25)
ttGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGcc > 2:3335793/1‑101 (MQ=255)
ttGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGcc < 2:609288/101‑1 (MQ=255)
tGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGccc > 1:1510700/1‑101 (MQ=255)
gg.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGcccc < 2:3325523/101‑1 (MQ=255)
gg.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGcccc > 2:1302425/1‑101 (MQ=255)
ccG.AAATCCGC.GGGGGGTCGGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcat < 2:241165/101‑1 (MQ=21)
ccG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGGCCcat > 2:3007974/1‑101 (MQ=25)
ccG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcat < 2:2800390/101‑1 (MQ=255)
g.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattc > 1:1883583/1‑101 (MQ=255)
aaaTCCGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattca < 2:590068/101‑1 (MQ=21)
aaaTACGC.CGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattca > 1:2444810/1‑101 (MQ=255)
aaaTACGC.CGGGGGTCCGGGGG.CAGCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattca < 2:4088520/101‑1 (MQ=21)
atccGC.GGGGGAACCGGTGG.AGGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcatt < 1:1384387/98‑1 (MQ=17)
atcgcgggggggCCGGGGGG.CGCCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcatt < 1:2488191/94‑1 (MQ=17)
ccgatctggggTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTg < 2:3900662/94‑1 (MQ=21)
aCGC.GGGGGGGGCGGGGG.CGCCGCCCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcatt < 1:3052168/101‑1 (MQ=17)
ggggggggTGCGGGGC.GCGCCCCCCGGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCa < 1:1048961/98‑1 (MQ=17)
c.cGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCAt > 2:3631603/1‑101 (MQ=255)
cGGGGGTCCGGGGG.CGCCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATa < 1:3393931/101‑1 (MQ=18)
cGGGGGTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATa > 2:3190725/1‑101 (MQ=255)
ggggTCCGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTgagatcgg > 1:3900921/1‑94 (MQ=18)
gcccGGGGG.CCCCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACg < 2:1088491/99‑1 (MQ=18)
gTCCGGGGG.CAGCGCCCCCTGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACg < 2:2047496/101‑1 (MQ=18)
cgggggg.CCGCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGAc < 1:1126617/99‑1 (MQ=18)
ccGGGGG.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGAc > 2:2731670/1‑101 (MQ=255)
ggggag.CGGCGCCCGGGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACa < 1:2842176/100‑1 (MQ=18)
cGGGGG.GAGCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACa < 1:718559/101‑1 (MQ=18)
cGGGGG.CAGCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACa < 2:3248461/101‑1 (MQ=21)
ggggg.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAg > 2:3830921/1‑101 (MQ=255)
ggg.CACCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGa < 2:2016499/101‑1 (MQ=18)
tg.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGAt > 2:2980874/2‑101 (MQ=18)
gcgccccccccGGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGAt < 2:1510507/94‑1 (MQ=18)
g.CAGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATg > 1:667676/1‑101 (MQ=255)
g.CAGCCCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATg < 1:1290345/101‑1 (MQ=18)
ccccccccccGGC...TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGc < 1:3701151/95‑1 (MQ=255)
gaGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGc < 2:544884/100‑1 (MQ=255)
aGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATgtg > 2:1870827/1‑99 (MQ=255)
aGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCg < 2:213112/101‑1 (MQ=255)
aGCGCCCCCGGC..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCg < 1:3831178/101‑1 (MQ=255)
gcccccGGC...TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAc > 1:2063886/3‑101 (MQ=255)
ccccGGC...TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCa > 2:283710/1‑101 (MQ=255)
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CGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGG.CCG.AAATACGC.CGGGGGTCCGGGGG.CAGCGCCCCGGCG..TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAT > NC_014034/1976802‑1976990
Base quality scores: 
ATCG
< 3 ≤
ATCG
< 19 ≤
ATCG
< 27 ≤
ATCG
< 34 ≤
ATCG
< 41 ≤
ATCG
|