Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,036,202 T→C 100% K48K (AAA→AAG iscS ← cysteine desulfurase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,036,2020T→C100.0% 627.8 165K48K (AAA→AAGiscScysteine desulfurase
Reads supporting (aligned to +/- strand):  new base (85/80):  ref base (0/0):  total (85/80)

Only 100 of 174 total aligned reads displayed.

CGACCGGCCAGCGCCAGCCCCGCCGCCTCGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCTTTTTCCATCAAGGGCTTGGGCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATCGCCGCCCTCGCCTCCG  >  NC_014034/2036104‑2036299
                                                                                                  |                                                                                                 
cgcccGGCCAGCCCCAGCCCCGCCGCCTCGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCctt                                                                                                 <  1:2790475/98‑1 (MQ=255)
    cGGCCAGCGCCAGCCCCGCCGCCTCGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTcc                                                                                             >  1:807596/1‑101 (MQ=255)
    cGGCCAGCGCCAGCCCCGCCGCCTCGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTcc                                                                                             <  1:1404770/101‑1 (MQ=255)
     ggCCAGCGCCAGCCCCGCCGCCTCGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCa                                                                                            <  2:2785751/101‑1 (MQ=255)
      gccagcgccagcCCCGCCGCCTCGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCAt                                                                                           >  2:3581295/1‑101 (MQ=255)
       ccagcgccagcCCCGCCGCCTCGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATc                                                                                          <  1:30054/101‑1 (MQ=255)
       ccagcgccagcCCCGCCGCCTCGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATc                                                                                          >  2:1863312/1‑101 (MQ=255)
       ccagcgccagcCCCGCCGCCTCGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATc                                                                                          >  2:945535/1‑101 (MQ=255)
       ccagccccagcCCCGCCGCCTCGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATc                                                                                          <  1:669102/101‑1 (MQ=255)
        cagcgccagcCCCGCCGCCTCGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCa                                                                                         >  1:2491537/1‑101 (MQ=255)
        cagccccagcCCCGCCGCCTCGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCa                                                                                         <  1:3498617/101‑1 (MQ=255)
        cagccccagcCCCGCCCCCTCGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCa                                                                                         <  1:3505043/101‑1 (MQ=255)
           ccccagcCCCGCCGCCTCGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAgg                                                                                      <  2:3235751/99‑1 (MQ=255)
            gccagcCCCGCCGCCTCGGTCGCGCCCGAGGCGTAAACAATAACCGCCCCCTCCCCCCCCAAAGCCCCCGCCCCCCGCTCGCGGGTCTTTTCCATCAAggc                                                                                     >  2:3832236/1‑100 (MQ=255)
             ccagcCCCGCCGCCTCGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAggcc                                                                                    <  1:2322509/101‑3 (MQ=255)
                gcCCCGCCGCCTCGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTg                                                                                 <  1:990026/101‑1 (MQ=255)
                 ccccGCCGCCTCGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTgg                                                                                <  2:2287565/101‑1 (MQ=255)
                    cgccgcCTCGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGccg                                                                             <  2:2773117/101‑1 (MQ=255)
                    cgccgcCTCGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGccg                                                                             <  1:2066912/101‑1 (MQ=255)
                     gccgccTCGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGccgc                                                                            >  2:287219/1‑101 (MQ=255)
                         ccTCGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCgg                                                                        <  2:4112117/101‑1 (MQ=255)
                            cGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGccc                                                                     <  1:3766277/101‑1 (MQ=255)
                            cGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGccc                                                                     >  1:1018181/1‑101 (MQ=255)
                             ggTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCt                                                                    <  1:2377874/101‑1 (MQ=255)
                                cgcgCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCgg                                                                 >  1:41295/1‑101 (MQ=255)
                                 gcgcCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGc                                                                <  1:529278/101‑1 (MQ=255)
                                  cgcCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCa                                                               >  2:1301170/1‑101 (MQ=255)
                                  cgcCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCa                                                               >  2:3884734/1‑101 (MQ=255)
                                    cccGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATg                                                             >  1:2731906/1‑101 (MQ=255)
                                      cGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGa                                                           >  1:2524776/1‑101 (MQ=255)
                                       gAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGAc                                                          <  1:1061326/101‑1 (MQ=255)
                                       gAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGAc                                                          >  2:3342282/1‑101 (MQ=255)
                                       gAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGAc                                                          >  1:3122762/1‑101 (MQ=255)
                                        gggTGAAGACGCTAGCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGAcc                                                         <  1:2750835/100‑1 (MQ=255)
                                         ggTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCg                                                        <  1:3082604/101‑1 (MQ=255)
                                         ggTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCg                                                        >  2:3839140/1‑101 (MQ=255)
                                          gTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGa                                                       <  1:2517063/101‑1 (MQ=255)
                                          gTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGa                                                       <  1:1932638/101‑1 (MQ=255)
                                            gAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGaaa                                                     >  2:3177337/1‑100 (MQ=255)
                                            gAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAg                                                     >  2:2373054/1‑101 (MQ=255)
                                               gACGATATCCTCCCCCTCCGCCCCCAAAGCCGCCGCCCCCTGCGAGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACg                                                  <  2:648355/101‑1 (MQ=255)
                                               gACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACg                                                  <  1:1451694/101‑1 (MQ=255)
                                                  gATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGAt                                               <  2:2974951/101‑1 (MQ=255)
                                                  gATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGAt                                               >  1:957416/1‑101 (MQ=255)
                                                   atatCCGCCCCCTCCGGCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGAtt                                              >  1:3698997/1‑101 (MQ=255)
                                                   atatCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGAtt                                              <  2:722290/101‑1 (MQ=255)
                                                       ccgccccctccgcccccAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGccc                                          >  2:268053/1‑101 (MQ=255)
                                                       ccgccccctccgcccccAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGccc                                          >  2:2333112/1‑101 (MQ=255)
                                                         gccccctccgcccccAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCAc                                        <  2:807518/101‑1 (MQ=255)
                                                          ccccctccgcccccAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACg                                       >  1:4112699/1‑101 (MQ=255)
                                                          ccccctccgcccccAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACg                                       <  2:2885301/101‑1 (MQ=255)
                                                          ccccctccgcccccAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACg                                       <  1:3167412/101‑1 (MQ=255)
                                                           cccctccgcccccAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGa                                      >  2:1948611/1‑101 (MQ=255)
                                                            ccctccgcccccAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGAc                                     <  2:2488758/101‑1 (MQ=255)
                                                             cctccgcccccAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGAcc                                    <  2:1674441/101‑1 (MQ=255)
                                                             cctccgcccccAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGAcc                                    <  1:2597442/101‑1 (MQ=255)
                                                              ctccgcccccAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCt                                   >  1:558150/1‑101 (MQ=255)
                                                              ctccgcccccAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCt                                   <  1:689601/101‑1 (MQ=255)
                                                              cgccgcccccAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCt                                   <  1:643563/99‑1 (MQ=255)
                                                               cccgcccccAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTc                                  <  2:3424738/100‑1 (MQ=255)
                                                                ccgcccccAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTcc                                 <  2:191806/101‑1 (MQ=255)
                                                                 cgccccaAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCa                                <  1:1440909/101‑1 (MQ=255)
                                                                  gcccccAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCAt                               >  2:2009710/1‑101 (MQ=255)
                                                                      ccAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATcgcc                           <  1:2792652/101‑1 (MQ=255)
                                                                      ccAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATcgcc                           >  2:2256410/1‑101 (MQ=255)
                                                                       cAAAGCCGCCGCGACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATcgccg                          >  1:2300759/1‑101 (MQ=255)
                                                                       cAAAGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATcgccg                          <  1:437062/101‑1 (MQ=255)
                                                                        aaaGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATcgccgc                         >  2:3938067/1‑101 (MQ=255)
                                                                         aaGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCa                        <  1:2572320/101‑1 (MQ=255)
                                                                          aGCCGCCGCCACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCaa                       >  1:1751948/1‑101 (MQ=255)
                                                                           gccgccgccACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAat                      >  2:1121329/1‑101 (MQ=255)
                                                                            ccgccgccACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAatc                     >  2:4150056/1‑101 (MQ=255)
                                                                               ccgccACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAatcatc                  <  2:2746785/101‑1 (MQ=255)
                                                                                cgccACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATcg                 <  1:4196789/101‑1 (MQ=255)
                                                                                cgccACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATcg                 <  2:587466/101‑1 (MQ=255)
                                                                                 gccACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATcgc                >  2:1566528/1‑101 (MQ=255)
                                                                                 gccACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATcgc                <  2:1299336/101‑1 (MQ=255)
                                                                                  ccACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATcgcc               <  2:1623215/101‑1 (MQ=255)
                                                                                   cACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATcgccg              <  2:2104949/101‑1 (MQ=255)
                                                                                   cACCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATcgccc              >  2:1032557/1‑100 (MQ=255)
                                                                                    aCCTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGGTTGCCCACGACCTCCATCGCCGCAAACATcgacac             >  2:2663727/1‑97 (MQ=255)
                                                                                     ccTGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATCgccgcc            <  1:1572690/101‑1 (MQ=255)
                                                                                       tGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGCCCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATCGCCGCCCt          <  1:3581548/101‑1 (MQ=255)
                                                                                       tGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATCGCCGCCCt          >  1:3559457/1‑101 (MQ=255)
                                                                                       tGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATCGCCGCCCt          <  2:3060959/101‑1 (MQ=255)
                                                                                       tGCGCGCGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATCGCCGCCCt          <  1:2633199/101‑1 (MQ=255)
                                                                                        gcgcgcGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATCGCCGCCCTc         >  2:966377/1‑101 (MQ=255)
                                                                                        gcgcgcGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATCGCCGCCCTc         <  1:1301266/101‑1 (MQ=255)
                                                                                        gcgcgcGGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATCGCCGCCCTc         <  2:1468047/101‑1 (MQ=255)
                                                                                            gcgGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATCGCCGCCCTCGCCt     >  1:1501481/1‑101 (MQ=255)
                                                                                            gcgGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATCGCCGCCCTCGCCt     <  2:1751829/101‑1 (MQ=255)
                                                                                            gcgGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATCGCCGCCCTCGCCt     >  2:165031/1‑101 (MQ=255)
                                                                                            gcgGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATCGCCGCCCTCGCCt     >  1:1936325/1‑101 (MQ=255)
                                                                                             cgGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATCGCCGCCCTCGCCTc    >  1:1091998/1‑101 (MQ=255)
                                                                                             cgGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATCGCCGCCCTCGCCTc    <  1:1767644/101‑1 (MQ=255)
                                                                                             cgGGCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATCGCCGCCCTCGCCTc    <  1:2335949/101‑1 (MQ=255)
                                                                                              gggCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATCGCCGCCCTCGCCTcc   >  2:2932394/1‑101 (MQ=255)
                                                                                              gggCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATCGCCGCCCTCGCCTcc   >  2:2186697/1‑101 (MQ=255)
                                                                                              gggCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATCGCCGCCCTCGCCTcc   >  1:654298/1‑101 (MQ=255)
                                                                                               ggCCTTTTCCATCAAGGCCTTGGCCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATCGCCGCCCTCGCCTCCg  <  2:2315801/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CGACCGGCCAGCGCCAGCCCCGCCGCCTCGGTCGCGCCCGAGGTGAAGACGATATCCGCCCCCTCCGCCCCCAAAGCCGCCGCCACCTGCGCGCGGGCTTTTTCCATCAAGGGCTTGGGCGCGCGGCCCTCGGCATGGACCGAAGACGGATTGCCCACGACCTCCATCGCCGCAATCATCGCCGCCCTCGCCTCCG  >  NC_014034/2036104‑2036299

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG