Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,040,375 C→G 3.4% intergenic (‑123/‑265) RCAP_rcc01888 ← / → RCAP_rcc01889 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,040,3750C→G 3.4% 7.8 0.0 ‑0.0203intergenic (‑123/‑265)RCAP_rcc01888/RCAP_rcc01889hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/3):  ref base (112/84):  total (116/87)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.42e-01

Only 100 of 221 total aligned reads displayed.

CCGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGTGGGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGG.C.GCGCCATAAGTGAGCCCACCCGGTTCGGAGGGTTGGATGCATAAATC  >  NC_014034/2040276‑2040476
                                                                                                   |                                                                                                       
ccGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCGgg                                                                                                       <  1:1669219/101‑1 (MQ=255)
 cGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCGggg                                                                                                      <  1:3224532/101‑1 (MQ=255)
  ggCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCGgggc                                                                                                     <  2:2143900/101‑2 (MQ=255)
  ggCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCGgggc                                                                                                     >  2:1885367/1‑100 (MQ=255)
     ctctGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGa                                                                                                 >  1:1184930/1‑101 (MQ=255)
      tctGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGAt                                                                                                >  2:3593029/1‑101 (MQ=255)
       ctGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATg                                                                                               >  1:2730955/1‑101 (MQ=255)
        tGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATgg                                                                                              >  1:3438861/1‑101 (MQ=255)
        tGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATgg                                                                                              >  2:2573552/1‑101 (MQ=255)
         gggTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGc                                                                                             >  2:2679762/1‑101 (MQ=255)
           gTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCtt                                                                                           <  2:362720/101‑1 (MQ=255)
            ttGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTa                                                                                          >  1:3336400/1‑101 (MQ=255)
            ttGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTa                                                                                          <  2:2318049/101‑1 (MQ=255)
             tGGGATGGAAAAAGACCGGAACGGCCCGTGGCCAGACGCATGCGT.GGGCGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAc                                                                                         <  2:1967172/101‑1 (MQ=255)
             tGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAc                                                                                         <  1:4065282/101‑1 (MQ=255)
                  tGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGt                                                                                    >  2:2951366/1‑101 (MQ=255)
                  tGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGt                                                                                    <  1:290313/101‑1 (MQ=255)
                   ggAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGGTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTa                                                                                   >  2:725179/1‑101 (MQ=255)
                    gAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTAt                                                                                  <  2:3916029/101‑1 (MQ=255)
                      aaaaGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATGCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATgg                                                                                <  2:1007216/101‑1 (MQ=255)
                      aaaaGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATgg                                                                                >  1:675003/1‑101 (MQ=255)
                           aCCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGcgcgg                                                                           >  2:1008186/1‑100 (MQ=255)
                           aCCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGt                                                                           >  2:595977/1‑101 (MQ=255)
                           aCCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGt                                                                           >  2:3982164/1‑101 (MQ=255)
                            ccGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTg                                                                          >  1:2809726/1‑101 (MQ=255)
                              ggAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGcc                                                                        >  2:2205550/1‑101 (MQ=255)
                              ggAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGcc                                                                        >  2:2481906/1‑101 (MQ=255)
                                aaCGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCaa                                                                      <  1:133563/101‑1 (MQ=255)
                                  cGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAg                                                                    <  2:1939707/101‑1 (MQ=255)
                                  cGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAg                                                                    <  1:554734/101‑1 (MQ=255)
                                   ggCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGa                                                                   >  1:1522672/1‑101 (MQ=255)
                                   ggCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGa                                                                   >  1:716890/1‑101 (MQ=255)
                                    gCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAc                                                                  <  2:3551816/101‑1 (MQ=255)
                                     ccAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAcg                                                                 <  2:2182245/101‑1 (MQ=255)
                                       aGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAcggc                                                               >  1:911024/1‑101 (MQ=255)
                                         gggCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAcggcgg                                                             >  1:942752/1‑101 (MQ=255)
                                            ccAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCg                                                          <  2:2022956/101‑1 (MQ=255)
                                            ccAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCg                                                          <  1:3593282/101‑1 (MQ=255)
                                             cAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGc                                                         <  1:104756/101‑1 (MQ=255)
                                             cAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGc                                                         >  1:2174453/1‑101 (MQ=255)
                                             cAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGc                                                         >  1:354269/1‑101 (MQ=255)
                                              aGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCa                                                        >  1:3455219/1‑101 (MQ=255)
                                               gACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCaa                                                       >  1:1326166/1‑101 (MQ=255)
                                                aCGCAGGCGT.GGGGGATCCGGTCGAAGGTCATACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCaaa                                                      <  1:3093195/101‑1 (MQ=255)
                                                aCGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCaaa                                                      >  1:4233687/1‑101 (MQ=255)
                                                 cGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAg                                                     >  2:1482210/1‑101 (MQ=255)
                                                  gCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAgg                                                    >  1:3650769/1‑101 (MQ=255)
                                                   cAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGg.c                                                  <  2:3455238/101‑1 (MQ=255)
                                                    aGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGg.cc                                                 >  2:2173822/1‑100 (MQ=255)
                                                       gcgtgggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGg.ccgc                                               >  2:3133713/5‑98 (MQ=255)
                                                        cgtgggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGg.ccgcg                                              <  1:1274969/98‑5 (MQ=255)
                                                        cgtgggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGg.ccgcg                                              >  2:3029638/4‑97 (MQ=255)
                                                          tgggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGGGGCCGCAAAGGCC.GCGcc                                            >  1:3850893/2‑101 (MQ=255)
                                                           gggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGCTGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCa                                           >  1:418527/1‑101 (MQ=255)
                                                           gggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCa                                           <  2:1104189/101‑1 (MQ=255)
                                                             gggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATa                                         <  1:2164575/101‑1 (MQ=255)
                                                             gggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATa                                         <  2:1151094/101‑1 (MQ=255)
                                                              ggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATaa                                        >  1:1466858/1‑101 (MQ=255)
                                                              ggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATaa                                        <  2:1076136/101‑1 (MQ=255)
                                                               gATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAg                                       >  1:823984/1‑101 (MQ=255)
                                                                aTCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGt                                      >  2:3288759/1‑101 (MQ=255)
                                                                   cGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAAGGCGGCCGCAAAGG.CGGCGCCATAAGTGAg                                   >  1:3875560/1‑101 (MQ=255)
                                                                   cGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGACGGATGGCTTACGCGGCATGGCGCGTGCCAAAGACGGAGGCCGCAAAGG.CTGCGCCATAAGTGAg                                   >  2:2823547/1‑101 (MQ=255)
                                                                       cGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCa                               >  1:3952228/1‑101 (MQ=255)
                                                                        gAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCAc                              >  2:1741044/1‑101 (MQ=255)
                                                                        gAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCAc                              >  1:2964253/1‑101 (MQ=255)
                                                                        gAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCAc                              >  1:2641295/1‑101 (MQ=255)
                                                                        gAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCAc                              <  1:2205709/101‑1 (MQ=255)
                                                                        gAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCAc                              <  1:35817/101‑1 (MQ=255)
                                                                          aGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCAccg                            <  2:474266/101‑2 (MQ=255)
                                                                          aGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCAccg                            <  1:2525905/101‑2 (MQ=255)
                                                                            gTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCAccggt                          >  1:1115544/1‑98 (MQ=255)
                                                                             tCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCAccggtt                         <  2:2730749/101‑5 (MQ=255)
                                                                             tCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCAccggtt                         <  2:3704789/101‑5 (MQ=255)
                                                                             tCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCAccggtt                         <  1:1000555/101‑5 (MQ=255)
                                                                              cAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTc                       <  1:1916028/101‑1 (MQ=255)
                                                                              cAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTc                       >  1:2045885/1‑101 (MQ=255)
                                                                               tccgatcttgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCg                      <  2:1720856/93‑1 (MQ=255)
                                                                               aGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCg                      <  2:835255/101‑1 (MQ=255)
                                                                                  cGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGAGGCCGCAAAGGCC.GCGCCATAAGTAAGCCCA.CCGGTTCgggg                   >  1:1408821/1‑99 (MQ=255)
                                                                                   gTTGTGCAAAAG.AACCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGGCGCAAAGGCC.GCGGCATAAGTGAGCCCA.CCGGTTCGGAgg                  >  2:1377728/1‑101 (MQ=255)
                                                                                     tgtgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGAggtt                >  1:163514/1‑99 (MQ=255)
                                                                                      gtgCAAAAGCA.CCGGGGCGGATGGCTTGCGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGAggttg               <  2:3438609/101‑4 (MQ=255)
                                                                                      gtgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGAggttg               <  2:1633290/101‑4 (MQ=255)
                                                                                       tgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCgagatcgga              >  1:1720971/1‑93 (MQ=255)
                                                                                       tgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGAggttgg              <  2:4133261/101‑5 (MQ=255)
                                                                                       tgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGAggttgg              >  2:1792704/1‑97 (MQ=255)
                                                                                        gCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGa            <  1:2370833/101‑1 (MQ=255)
                                                                                        gCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGa            >  2:2083078/1‑101 (MQ=255)
                                                                                        gCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGa            >  2:3908466/1‑101 (MQ=255)
                                                                                        gCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGa            >  2:818119/1‑101 (MQ=255)
                                                                                         cAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGAt           >  2:1302553/1‑101 (MQ=255)
                                                                                          aaaaGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGG.CGGCGCCATAAGTGAGGCCA.CCGGTTGCGA.GGTTGGATg          >  2:3777963/1‑101 (MQ=255)
                                                                                           aaaatcaccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATg          <  2:1330938/94‑1 (MQ=255)
                                                                                           aaaGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATgt         <  2:3871574/101‑2 (MQ=255)
                                                                                            aaGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCa        >  1:1791981/1‑101 (MQ=255)
                                                                                               gcaccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATa      >  2:2647745/4‑101 (MQ=255)
                                                                                                caccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATaa     <  2:2964031/99‑1 (MQ=255)
                                                                                                 accGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATaaa    >  2:1539969/2‑101 (MQ=255)
                                                                                                  ccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAAt   >  1:4033326/1‑101 (MQ=255)
                                                                                                   |                                                                                                       
CCGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGTGGGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGG.C.GCGCCATAAGTGAGCCCACCCGGTTCGGAGGGTTGGATGCATAAATC  >  NC_014034/2040276‑2040476

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG