BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,057,834	A→C	20.7%	intergenic (‑600/‑302)	RCAP_rcc01900 ← / → RCAP_rcc01901	hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,057,834	0	A→C	20.7%	87.0 ‑0.9 ‑0.5	150	intergenic (‑600/‑302)	RCAP_rcc01900/RCAP_rcc01901	hemolysin‑type calcium‑binding repeat family protein/polyphosphate kinase 2 domain‑containing protein
Reads supporting (aligned to +/- strand): new base (17/14): ref base (54/65): total (71/79)
Fisher's exact test strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.22e-01

Only 100 of 158 total aligned reads displayed.


CTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.CGCG.C.GC.A.GC.G.TTCCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057735‑2057913


cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.C.GC.C.Gc                                                                                  >  1:3507570/1‑101 (MQ=255)


cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.C.GC.C.Gc                                                                                  >  2:3934407/1‑101 (MQ=255)


cTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.C.GC.C.Gc                                                                                  >  2:3444635/1‑101 (MQ=255)


 tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.C.GC.C.Gca                                                                                 <  1:2563092/101‑2 (MQ=255)


 tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.C.GC.C.Gca                                                                                 >  2:3617028/1‑100 (MQ=255)


 tGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.C.GC.C.Gca                                                                                 <  1:610276/101‑2 (MQ=255)


  gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.C.GC.C.Gcaa                                                                                >  2:2581873/1‑99 (MQ=255)


  gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.C.GC.C.Gcaa                                                                                >  2:2633318/1‑99 (MQ=255)


  gTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.C.GC.C.Gcaa                                                                                <  2:2168895/101‑3 (MQ=255)


   tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.C.GC.C.Gcaag                                                                               >  1:3315584/1‑98 (MQ=255)


   tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.C.GC.C.Gcaag                                                                               >  1:2073121/1‑98 (MQ=255)


   tttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.C.GC.C.Gcaag                                                                               <  1:267659/101‑4 (MQ=255)


    ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.C.GC.C.Gcaagc                                                                              <  2:3118739/101‑5 (MQ=255)


    ttACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.C.GC.C.Gcaagc                                                                              >  1:2668288/1‑97 (MQ=255)


     tACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.C.GC.C.Gcaagcg                                                                             >  1:4138538/1‑96 (MQ=255)


      aCCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.C.GC.C.Gcaagcgt                                                                            >  1:2021460/1‑95 (MQ=255)


      aCCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.C.GC.C.Gcaagcgt                                                                            <  2:217910/101‑7 (MQ=255)


      aCCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.C.GC.C.Gcaagcgt                                                                            >  1:370906/1‑95 (MQ=255)


       ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.C.GC.C.Gcaagcgtt                                                                           <  1:298756/101‑8 (MQ=255)


       ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.C.GC.C.Gcaagcgtt                                                                           <  2:366527/101‑8 (MQ=255)


       ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.C.GC.C.Gcaagcgtt                                                                           <  2:3021485/101‑8 (MQ=255)


       ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.C.GC.C.Gcaagcgtt                                                                           <  1:143419/101‑8 (MQ=255)


       ccGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.C.CG.C.GC.C.Gcaagcgtt                                                                           >  1:505695/1‑94 (MQ=255)


                aaCCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCg                                                                     >  2:289009/1‑101 (MQ=255)


                    cTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCAt                                                                 >  1:336033/1‑101 (MQ=255)


                     tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACACTAC.CCCGCC.GCAA.GCGG.TTCCGAACCGGCATc                                                                >  2:1560836/1‑101 (MQ=255)


                     tGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATc                                                                <  2:3414405/101‑1 (MQ=255)


                      gCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCa                                                               >  2:1679883/1‑101 (MQ=255)


                        ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCatt                                                             >  2:2765985/1‑99 (MQ=255)


                        ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCatt                                                             <  2:3502260/101‑3 (MQ=255)


                        ttCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.CGCGCGCC.GCAA.GC.GTTTCCGTACCGGCATCatt                                                             >  2:380146/1‑99 (MQ=255)


                          cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGCACCGGCATCATTCt                                                           >  2:3090802/1‑101 (MQ=255)


                          cGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCt                                                           >  1:893358/1‑101 (MQ=255)


                           ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGGGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCtt                                                          >  2:3710556/1‑101 (MQ=255)


                           ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCtt                                                          <  1:354524/101‑1 (MQ=255)


                           ggCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCtt                                                          >  1:2662122/1‑101 (MQ=255)


                            gcgcgcGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTg                                                         <  1:4108495/101‑1 (MQ=255)


                              gcgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTCG.c                                                      <  1:3126361/101‑1 (MQ=255)


                               cgcgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGG.cc                                                     <  1:684997/101‑1 (MQ=255)


                                 cgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGG.cccc                                                   >  2:3867871/1‑101 (MQ=255)


                                 cgGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGG.cccc                                                   <  2:858906/101‑1 (MQ=255)


                                  ggACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGG.ccccc                                                  >  2:328115/1‑101 (MQ=255)


                                   gACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGG.cccccc                                                 <  2:2506568/101‑2 (MQ=255)


                                    aCTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGG.ccccccg                                                <  2:2372330/101‑3 (MQ=255)


                                       tCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGG.ccccccgccg                                             <  1:3091032/101‑6 (MQ=255)


                                           ggcggcggcGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGAc                                          <  2:1090831/101‑1 (MQ=255)


                                           ggcggcggcGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGAc                                          >  2:3995263/1‑101 (MQ=255)


                                           ggcggcggcGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGAc                                          <  1:1876978/101‑1 (MQ=255)


                                             cggcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACaa                                        <  1:1743740/101‑1 (MQ=255)


                                              gggggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAAt                                       <  1:1679998/98‑1 (MQ=255)


                                               gcggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATc                                      <  2:4226230/101‑1 (MQ=255)


                                                 ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                    <  2:505638/101‑1 (MQ=255)


                                                 ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                    >  2:2913539/1‑101 (MQ=255)


                                                 ggcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCAc                                    >  1:2333424/1‑101 (MQ=255)


                                                  gcggGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACg                                   >  2:2692038/1‑101 (MQ=255)


                                                    ggggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgc                                 >  1:50404/1‑101 (MQ=255)


                                                     gggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgcc                                <  2:4079203/101‑1 (MQ=255)


                                                      ggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccg                               <  2:1515937/101‑1 (MQ=255)


                                                      ggCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccg                               <  2:930627/101‑1 (MQ=255)


                                                        ctctTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGgccgcc                             >  1:246402/1‑101 (MQ=255)


                                                          ctTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTc                           >  1:67894/1‑101 (MQ=255)


                                                          ctTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTc                           <  1:1565051/101‑1 (MQ=255)


                                                          ctTGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGACCCCCGCCGGGACAATCACGGCCGCCTc                           >  1:3981299/1‑101 (MQ=255)


                                                           ttGCGATCCTGCGTTTCCGGATTGGCGACATTCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCa                          <  1:3843217/101‑1 (MQ=255)


                                                           ttGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCa                          >  1:1175039/1‑101 (MQ=255)


                                                            tGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACgagatcggaag                         >  1:827112/1‑91 (MQ=255)


                                                            tGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         >  2:975240/1‑101 (MQ=255)


                                                            tGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         >  2:81802/1‑101 (MQ=255)


                                                            tGCGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCAAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCAt                         <  2:258077/101‑1 (MQ=255)


                                                              cGATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTg                       >  2:1934730/1‑101 (MQ=255)


                                                               gATCCTTCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GCTT.TTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGt                      <  2:2056094/101‑1 (MQ=255)


                                                               gATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGt                      <  1:3009166/101‑1 (MQ=255)


                                                               gATCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGt                      >  1:1431432/1‑101 (MQ=255)


                                                                 tCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    >  2:3824556/1‑101 (MQ=255)


                                                                 tCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    >  1:3698311/1‑101 (MQ=255)


                                                                 tCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    <  1:20331/101‑1 (MQ=255)


                                                                 tCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTc                    >  1:1254049/1‑101 (MQ=255)


                                                                 tCCTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GC.ACGC.GTTTCCGAACCGGCATCGTTCTTGGCCCCCCGCCGGGTCAATCACGGCCGCCTCATTGTTc                    <  1:3617281/101‑1 (MQ=255)


                                                                   cTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCg                  <  1:1592229/101‑1 (MQ=255)


                                                                   cTGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCg                  >  1:2142521/1‑101 (MQ=255)


                                                                    tGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATTATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGc                 >  1:3958023/1‑101 (MQ=255)


                                                                    tGCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGc                 <  1:2844047/101‑1 (MQ=255)


                                                                     gCGTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGcc                >  1:2787939/1‑101 (MQ=255)


                                                                       gTTTCCGGGTTGGCGACAATCC.CGCG.CGGCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt              <  2:2012988/101‑1 (MQ=255)


                                                                       gTTTCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCAt              >  2:3313142/1‑101 (MQ=255)


                                                                         ttCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTg            <  1:27735/101‑1 (MQ=255)


                                                                          tCCGGATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGc           <  2:733127/101‑1 (MQ=255)


                                                                             ggATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGAt        >  2:1549081/1‑101 (MQ=255)


                                                                              gATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCCTTGTTCCGCCATTGCGATc       >  1:2374404/1‑101 (MQ=255)


                                                                              gATTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATc       <  1:938238/101‑1 (MQ=255)


                                                                               aTTGGCTACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      <  2:1809245/101‑1 (MQ=255)


                                                                               aTTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      >  2:3212836/1‑101 (MQ=255)


                                                                               aTTGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCa      >  1:1739599/1‑101 (MQ=255)


                                                                                ttGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaa     >  2:12378/1‑101 (MQ=255)


                                                                                 tGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa    <  1:2720854/101‑1 (MQ=255)


                                                                                 tGGCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCaaa    <  2:2540734/101‑1 (MQ=255)


                                                                                  ggCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAg   >  2:3204027/1‑101 (MQ=255)


                                                                                  ggCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAg   >  2:1423631/1‑101 (MQ=255)


                                                                                   gCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGACCCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGc  <  1:1867937/101‑1 (MQ=255)


                                                                                   gCGACAATCC.CGCGCC.GCAA.GC.GTTTCCGAACCGGCATCATTCTTGGCCCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGc  <  2:778283/101‑1 (MQ=255)


CTGTTTACCGCCCATTAACCCTGCTTCGGCGCGCGGACTTCGGGGCGGCGGCGGGGCTCTTGCGATCCTGCGTTTCCGGATTGGCGACAAT.C.CGCG.C.GC.A.GC.G.TTCCGAACCGGCATCAATCTTGG.CCCCCGCCGGGACAATCACGGCCGCCTCATTGTTCCGCCATTGCGATCAAAGC  >  NC_014034/2057735‑2057913

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 26 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG