Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,155,440 G→C 10.4% S2T (AGC→ACC)  RCAP_rcc02000 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,155,4400G→C10.4% 41.2 ‑1.5 ‑0.1183S2T (AGC→ACC) RCAP_rcc02000hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/13):  ref base (80/84):  total (86/97)
Fisher's exact test strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.73e-01

Only 100 of 188 total aligned reads displayed.

CGCCGACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACC  >  NC_014034/2155341‑2155540
                                                                                                   |                                                                                                    
cgcccccccGCCCGCGGTCCCCCCCCCCTTCTGATGCCCTCGGGCGCCGTCCCCGTCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGc                                                                                                     <  1:764117/95‑1 (MQ=255)
 cccgCCCCGGCCGCGGTCCCCCTCCCCTTCTGATGCCCTCGGGCGCCGTCACCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCg                                                                                                    <  2:3735640/100‑1 (MQ=255)
  ccgCCCGGGCCGCGGTCCCCCTCCCCTTCTGATGCCCTCGGGCGCCGTCCCCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGa                                                                                                   <  2:3018122/101‑1 (MQ=255)
  cagACCCGGCCGCGGTCCCCCTCCTCTTCTGACCCCCTCGGGCGCCGTCACCGTCACCGCCCGCGGGGCGCAAAGCCGCGAAAGGGCAGGATCATGAGCGa                                                                                                   <  2:1247487/99‑1 (MQ=255)
   cgccccGGCCGCGGCCGCCCTCCCTTTCTGATGCCCTCGGGCCCCGCCACCGCCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGAt                                                                                                  <  2:959786/98‑1 (MQ=255)
    gccccGGCCGCGGTCGCCCTCCCCTTCTGATGCCCTCGGGCCCCGTCCCCGCCCCCGCCCGCCGGGCGGCAAGCCGCGATAGGGCAGGATCATGAGCGATg                                                                                                 <  1:2403025/99‑1 (MQ=255)
    gACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATg                                                                                                 >  2:4024758/1‑101 (MQ=255)
    gACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATg                                                                                                 >  2:1395040/1‑101 (MQ=255)
     ccccGCCCGCGGTCGCCCTCCCCTTCTGATGCCCTCGGGCGCCGCCCCCGCCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGc                                                                                                <  1:3562541/100‑1 (MQ=255)
      cccGGCCGGGGTCCCCCTCCCCTTCTGATCCCCTCGGGCGCCGTCCCGGTCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCa                                                                                               <  2:1593872/101‑1 (MQ=255)
       cggtcctcgGTCGCCCTCACCTTCTGATTCCCTCGGGCCCCGTCACCGTCCCCGCCCCCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCaa                                                                                              <  1:1992282/94‑1 (MQ=255)
       ccgccggcgGTCCCCCCACCCTTCAGGTGCCCACGGGCGCCGCCGCGGACCCCGCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCaa                                                                                              <  2:3112998/95‑1 (MQ=255)
        cgcccGCGGCCCCCCTCCCTTTCTGATCCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCGGGGCGGAAAGCCGCGAAAGTGCAGGATCATGAGCGATGCAAg                                                                                             <  2:3893011/98‑1 (MQ=255)
        cGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAg                                                                                             >  2:2956563/1‑101 (MQ=255)
              gggccGCCCCCCCCTCCTCTTGCCTGCGGCCCCCGCCCCCGGCGCCGCCCCCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCt                                                                                       <  1:1188311/97‑1 (MQ=255)
              cgGTCCCCCCCCCCTTCTGATCCCCTCGGGCGCCGCCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCt                                                                                       <  1:3048440/101‑1 (MQ=255)
               gcccccccTCCCCTTCTGATGCCCCGGGGCGCCGTCCCCGTCCCCCCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTc                                                                                      <  2:3201501/96‑1 (MQ=255)
                gTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCg                                                                                     >  1:4050195/1‑101 (MQ=255)
                   gCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTag                                                                                  >  1:4153923/1‑101 (MQ=255)
                   gCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGACACCGCCCCCCCGGCGGCAACGCGGGAAAGGGCAGCAGCATGAGCGATGCAAGGGGACACGTag                                                                                  >  2:506512/1‑101 (MQ=255)
                   ccccTCCCTTTTTGATGCCCACGGGGGCCGTCCCCTCCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTag                                                                                  <  1:266174/100‑1 (MQ=255)
                    ccccccccTTCTGATCCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTaga                                                                                 <  1:4110374/95‑1 (MQ=255)
                    cccTCCCCTTCTGATGCCCTCGGGCGCCGTCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTaga                                                                                 <  1:2745070/101‑1 (MQ=255)
                      ccccccTTCTGATCCCCTCGGGCCCCGCCCCCGTCCCCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGc                                                                               <  1:1043147/97‑1 (MQ=255)
                      ccccccTTCTGATCCCCCCGGGCGCCGCCCCCGCCCCCCCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGc                                                                               <  1:1595350/97‑1 (MQ=255)
                      cTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGc                                                                               >  1:2146572/1‑101 (MQ=255)
                      cTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAAGGGCCTCGTAGAGc                                                                               >  2:2819695/1‑101 (MQ=255)
                       tCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCt                                                                              >  1:29183/1‑101 (MQ=255)
                         cccTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGACAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcg                                                                            <  2:3342103/100‑1 (MQ=255)
                           cTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCtcgtc                                                                          >  1:526955/1‑101 (MQ=255)
                             tCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGa                                                                        >  2:1283786/1‑101 (MQ=255)
                               tGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATg                                                                      >  2:2348429/1‑101 (MQ=255)
                               tGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATg                                                                      >  2:2647920/1‑101 (MQ=255)
                                 aTGCCCTGGGGCCCCGCCCCCGTCCCCGCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGcc                                                                    <  2:3089802/101‑1 (MQ=255)
                                   ccccTCGGGCGCCGTCCCCGCCCCCGCCCGCGGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCAt                                                                  <  2:370273/100‑1 (MQ=255)
                                    cccCCGGGCGCCGTCCCCGCCCCCCCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATc                                                                 <  1:342610/101‑1 (MQ=255)
                                        cGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggg                                                             >  2:2570574/1‑101 (MQ=255)
                                          gcccccgtccccgcccCCGCCCGCCGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggc                                                           <  2:4148143/97‑1 (MQ=255)
                                           gcgcCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcg                                                          >  1:201703/1‑101 (MQ=255)
                                           gcgcCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcg                                                          >  2:3220082/1‑101 (MQ=255)
                                            cgcCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcgg                                                         >  2:1851147/1‑101 (MQ=255)
                                             gcCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTggggcggg                                                        >  1:1721160/1‑101 (MQ=255)
                                                gtcaccgtcaccGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGcc                                                     >  1:827635/1‑101 (MQ=255)
                                                ctccccgtccccgCCCGCGGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGcc                                                     <  2:639534/97‑1 (MQ=255)
                                                  caccgtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGt                                                   >  1:846853/1‑101 (MQ=255)
                                                   ccctcccccccccgcgGGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTc                                                  <  1:2956785/91‑1 (MQ=255)
                                                    cgttcgccgcccggGGGGCGCCAAGCCGCGAAAGGGGAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCGCGACGATGCCATCTGGGGCGGGGCCTTca                                                 <  2:3475655/95‑1 (MQ=255)
                                                      gtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                               >  2:840324/1‑101 (MQ=255)
                                                      gtcaccgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                               >  2:2997949/1‑101 (MQ=255)
                                                      ccccccccccgcgGGGCGAAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacc                                               <  2:827554/94‑1 (MQ=255)
                                                       cccccccccgccGGGCGAAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTcacca                                              <  2:2707371/95‑1 (MQ=255)
                                                         accgCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCCCCACg                                            >  2:2874651/1‑101 (MQ=255)
                                                          ccgcccgcCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGa                                           >  1:672984/1‑101 (MQ=255)
                                                            gcccgccGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGGCGTCACCACGATg                                         >  2:267955/1‑101 (MQ=255)
                                                             cccgcgGGGGCGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATgg                                        <  2:3971114/101‑1 (MQ=255)
                                                              ccgccgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggc                                       >  2:2874784/1‑101 (MQ=255)
                                                               cgccgGGCGGAAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcg                                      <  1:2505706/101‑1 (MQ=255)
                                                                  cgGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcg                                   >  2:354705/1‑101 (MQ=255)
                                                                   gggCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATggcggcgg                                  >  2:3789401/1‑101 (MQ=255)
                                                                       ggcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCt                              >  1:1625843/1‑101 (MQ=255)
                                                                        gcAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                             <  2:1695898/101‑1 (MQ=255)
                                                                        gaaaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTc                             <  2:3496777/99‑1 (MQ=255)
                                                                          atctcgcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGc                           <  2:3634651/97‑1 (MQ=255)
                                                                          aaGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGc                           >  1:2180979/1‑101 (MQ=255)
                                                                           aGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGcc                          >  1:416624/1‑101 (MQ=255)
                                                                             ccGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGGGCTCGCCgg                        >  2:1089276/1‑101 (MQ=255)
                                                                              cgcgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCggg                       <  2:2540775/101‑1 (MQ=255)
                                                                                cgAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcg                     >  2:543223/1‑101 (MQ=255)
                                                                                 gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                    >  1:813513/1‑101 (MQ=255)
                                                                                 gAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcgg                    >  1:348982/1‑101 (MQ=255)
                                                                                  aaaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGggcggc                   >  1:3274361/1‑101 (MQ=255)
                                                                                   aaGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCt                  <  1:1851267/101‑1 (MQ=255)
                                                                                    aGGGCAGGATCATGAGCGATGCAAGGGTCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTg                 >  1:2885760/1‑101 (MQ=255)
                                                                                     gggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGa                >  2:2158109/1‑101 (MQ=255)
                                                                                      ggCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAt               <  1:423630/101‑1 (MQ=255)
                                                                                      gagaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAt               <  1:4179696/92‑1 (MQ=255)
                                                                                       gCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtg              >  2:3569839/1‑101 (MQ=255)
                                                                                       agaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtg              >  2:681350/9‑101 (MQ=255)
                                                                                        gaaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgt             <  2:3314312/94‑1 (MQ=255)
                                                                                        cAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgt             <  2:78712/101‑1 (MQ=255)
                                                                                        cAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgt             <  1:2105713/101‑1 (MQ=255)
                                                                                         aaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgtg            <  1:398526/95‑1 (MQ=255)
                                                                                         aaaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGAtgtg            <  2:1306654/95‑1 (MQ=255)
                                                                                         aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgtg            <  1:2648126/101‑1 (MQ=255)
                                                                                         aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGAtgtg            >  2:430638/1‑101 (MQ=255)
                                                                                         aGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCCCCGGGCGGCTGAtgtg            >  1:50327/1‑101 (MQ=255)
                                                                                          ggATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTgg           <  2:2392573/101‑1 (MQ=255)
                                                                                          aaaaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTgg           >  1:3722990/6‑101 (MQ=255)
                                                                                           gATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGc          <  1:3140995/101‑1 (MQ=255)
                                                                                            aaaaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCa         >  1:2451222/4‑101 (MQ=255)
                                                                                            aTCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCa         >  1:967259/1‑101 (MQ=255)
                                                                                              cATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGGTGTGGCAtt       >  1:145185/1‑101 (MQ=255)
                                                                                              aaTGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCAtt       <  1:1044687/100‑1 (MQ=255)
                                                                                               aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCAttg      >  2:555857/1‑100 (MQ=255)
                                                                                               aTGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTc      <  2:1721045/101‑1 (MQ=255)
                                                                                                tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg     <  2:201665/101‑1 (MQ=255)
                                                                                                tGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCg     <  2:2307370/101‑1 (MQ=255)
                                                                                                tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg     <  2:1977537/101‑1 (MQ=255)
                                                                                                tGACCGATACCAGAGGCCTCGTCGAGGTCATCGATGCCCTTTGTGGTGGCGCCATCACGACGATGGCGGCGGCGCTGGCCGGGCGCCTGATGTGGCATTCg     <  1:3824183/101‑1 (MQ=255)
                                                                                                 gAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGa    <  2:6103/101‑1 (MQ=255)
                                                                                                   |                                                                                                    
CGCCGACCCGGCCGCGGTCGCCCTCACCTTCTGATGCCCTCGGGCGCCGTCACCGTCACCGCCCGCCGGGCGGCAAGCCGCGAAAGGGCAGGATCATGAGCGATGCAAGGGGCCTCGTAGAGCTCGTCGATGCCATCTGGGGCGGGGCCGTCACCACGATGGCGGCGGCGCTCGCCGGGCGGCTGATGTGGCATTCGACC  >  NC_014034/2155341‑2155540

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG